IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ basu-2005-1 DISTORTED3/SCAR2 is a putative arabidopsis WAVE complex subunit that activates the Arp2/3 complex and is required for epidermal morphogenesis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Basu D., Le J., El-Essal Sel-D., Huang S., Zhang C., Mallery EL., Koliantz G., Staiger CJ., Szymanski DB. dszyman@purdue.edu Plant Cell (1040-4651) 2005 basu-2005-2 DISTORTED3/SCAR2 is a putative arabidopsis WAVE complex subunit that activates the Arp2/3 complex and is required for epidermal morphogenesis. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Basu D., Le J., El-Essal Sel-D., Huang S., Zhang C., Mallery EL., Koliantz G., Staiger CJ., Szymanski DB. dszyman@purdue.edu Plant Cell (1040-4651) 2005 abil1_arath Protein ABIL1 Abl interactor-like protein 1 ABIL1 T3F17 At2g46225 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress METEISGMDNPAMTLDEVSMERNKSFVKALQELKNLRPQLYSAADYCEKSYLHSEQKQMVLDNLKDYTVKALVNAVDHLGTVASKLTDLFDHQNSDISTMEMRASCVSQQLLTCRTYIDKEGLRQQQLLAVIPLHHKHYILPNSVNKRVHFSPLRRTDTRQNHYQAISRLQPSDAPASKSLSWHLGSETKSTLKGTSTVAPSSKDSKAFSKTSGVFHLLGDDENIANKKPLAGSQVSGVPAASTAHKDLEVPKLLTAHRSLDNNPRREIIQAPVRTKSVLSAFFVKQKTPKLKAGYVS nckp1_arath Protein NAP1 Nck-associated protein 1 p125Nap1 NAP of plants ARP2/3 regulatory protein subunit NAPP Protein GNARLED NAP1 GRL NAP NAPP T4C15.22 At2g35110 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANSRQYYPSQDESMSPTSVRSREWEGPSRWTEYLGPEMAASVSSTRSSKQIDGHVGGSTKALNIQWVVQMIEVADGLMAKMYRLNQILEYPDPVGHVFSEAFWKAGVFPNHPRICTLLSKKFPEHFSKLQLERIDKFSLDSLHDGAELHLQSLEPWIQLLLDLMAFREQALRLILDLSSTVITLLPHQNSLILHAFMDLFCAFVRVNLFAEKIPRKMLLQVYNLLHALSRNDRDCDFYHRLVQFIDSYDPPLKGLQEDLNFVSPRIGEVLEAVGPSIFLSADTRKLRNEGFLSPYHPRFPDILTNSAHPMRAQDLANVTSYREWVLLGYLVCPDELLRVTSIDIALVVLKENLVVTLFRDEVSLYQMVCEKEFGIGISFASADSINLTMQYILLHEDYQLYVLPRVLESKKMAKSGRTKQKEADLEYSVAKQVEKMISEVHEQALQLCDTIHRERRILLKQEIGRMVLFFTDQPSLLAPNIQMVFSALALAQSEVLWYFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQHLTTVFRNTMFGPEGRPQHCCAWLSVASSFPECASLIIPEEVTKFGRDAVLYVESLIESIMGGLEGLINILDSEGGFGALESQLLPEQAAAYLNNASRISAPSVKSPRVVGGFTLPGHESYPENNKSIKMLEAAIQRLTNLCSILNDMEPICVINHVFVLREYMRECILGNFKRRFLTALQTDNDLQRPSVLESLIRRHMGIVHLAEQHVSMDLTQGIREILLTEAFSGPVSSLHTFEKPAEQQQTTGSAVEVVCNWYMDNIIKDVSGAGILFAPRHKYFKSTRPVGGYFAESVTDLKELQAFVRIFGGYGVDRLDRMMKVHTAALVNCIETSLRSNRELIEAAAASMHSGDRVERDASVRQIVDLDTVIGFCIEAGQALAFDDLLAEASGAVLEDNASLIHSMISGIVEHIPEEIPEKKEIRRIKGVANGVGVAGDHDSEWVRLILEEVGGANDNSWSLLPYFFASFMTSNAWNTTGFNIETGGFSNNIHCLARCISAVIAGSEYVRLQREYQQQHQSLSNGHHSSENLDSEFPPRVTAEASIKSSMLLFVKFAASIVLDSWSEANRSHLVAKLIFLDQLCEISPYLPRSSLESHVPYTILRSIYTQYYSNTPSTPLSTASPYHSPSVSLIHASPSMKNSTTPQRGSGSGSSSTAAPDSGYFKGSSSSLYGQEHYTESETGNSRNNENNNNNKQRGSSRRSGPLDYSSSHKGGSGSNSTGPSPLPRFAVSRSGPISYKQHN nckp1_human Nck-associated protein 1 NCKAP1 HEM2 KIAA0587 NAP1 p125Nap1 Membrane-associated protein HEM-2 protein protein human Homo sapiens Human MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA scar2_arath Protein SCAR2 WAVE4 Protein WAVE4 Protein DISTORTED 3 Protein IRREGULAR TRICHOME BRANCH 1 SCAR2 DIS3 ITB1 T19C21.7 At2g38440 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPLTRYQSRNEYGLADPDLYQAADKDDPEALLEGVAMAGLVGILRQLGDLAEFAAEMFHDLHEEVMATASRSHGLMARVQQLEAEFPSIEKALLCQTDHSPFFSNKGVEWHPNLQLEQSVVTSGDLPRCVMDSYEECRGPPRLFLLDKFDISGAGACLKRYTDPSFVRLETSSYEESWDDIQREKKSQKAKRRASQWRNGGTPENALSSHAKLHELFLEEHLEAHHSDPARVVKLKTRKLDGCSLISKSGESYMEKFVQTRVDSKISYEIITQNPGLLTWNMDSARDVVTDIPEISMVGAMDKSHGGSRAEVSFPSEQENVANVNMNGGFIEKDIETVPESTYNEVRGTTITQDSQTVLNGKPGFFQQRSYSEDLTSEADNYVDAPATMESETETDDECRPKSRSDTLKDGNHHIYSDAVEERMEDPPQFSFSHSNGNTPVSENGRSSFGKKSTSYSYSDTASISIDDQSDGEKLSGCLPSTSSFKSELVDSMSHVTPEANKVSHDLNVQESVSSSNVDGQTSLSSNGTCSSPRPVSQNDQSCSLTVQSLASEVVETSPELVRLDLMKGGNDGRKVDPFDSSKSCASFDAKNSDLPSETSSISSTSEGSRCDSTIEKNCMVASNLVNSGTSPQAFVDSQTGKQLPIADTDFETNSIVACSEVLANSGSDPEERDGRCLTGKLVPCSAGVGMEVSPDTPSKVCGPSSADGIHLKDTLDDETDCVSVTNVVVDVDSKNSVADVGSQSSVADIDSQSSVAEISDEHSCAFGNTADVSVSESHEDTLENGMSVPSDFNSGVEKLAGDASPTCSKCDDHISHEGFHDLSGLDNATTDIVPNVELDVSDNDNDTSSGGVNHAVSLSSTRGKGSLPWISTNTYQSSSDAGEIFHDTVVESDGTLLEDNNPESEIKMHKSPLEVSSEGLSTEPDNKDVESIESTSPKPSLDQRNRDTETKSPGESILDDNCIDSTQVYNLNLLESEAIDQAVREQTSYASHEVADEELLQSNVFRGLEFEPQSAGLEFAPQSAGIELNRPKQELNLDPTFPSFGFIPETIPPNPEDMPPLPPMQWLIGKVPHSFPTFMGESVETSSSALSAAPPIGSSLNVQIGSPPSELSVSLGSDESERLPGGFVHNASEKPLQSSIQFPTMSTDLNSQYDSSELPTIPYQECIEDFGSEENNLLADHAAQNHELVYSQASSLQLPQVKHEDFKDDADVHESQSSSDDHHCPETKSLTPTQSTKVEDKGHSVPDASNAETAESSNTSVQKINPVSVGDAMWPVSCFSVAPTLDTYKTEVVPTVRLPRPRSPLVDAVAAHDRRKMKKVSEMVHPPIKSKQDDKDSLLAQIRNKSVNLKPAVTTRPSIQTGPRTDLRVAAILEKANTIRMAMAGSDEDEDSDSWSDS arpc3_arath Actin-related protein 2/3 complex subunit 3 ARPC3 Actin-related protein C3 Arp2/3 complex 21 kDa subunit T13D8.30 At1g60430 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVYHSSFVDEEGVTKACGCPLLPLKSHIKGPAPVSEQDKTDIVDEAITFFRANVFFTNFDIKSPADKLLIYLTFYINVALKRLEGCRTLAVGTKAIINLGLEDIPVPGETGFPFPGLFSLPQSQDEAELFRNYLKQVREETSGRLLSVAYRANGTPNKWWLAFAKRKFMNVVVL wasf1_human Wiskott-Aldrich syndrome protein family member 1 WASF1 KIAA0269 SCAR1 WAVE1 Protein WAVE-1 Verprolin homology domain-containing protein 1 protein protein human Homo sapiens Human MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPPPPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVTALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE abil1-nap1-1 Interaction between ABIL1 and NAP1 proteins 673598 N/A 673600 unspecified role unspecified role bait bait N/A 673601 unspecified role unspecified role prey prey physical association physical association false false false 10 A abil1-nckap1 Interaction between ABIL1 and Human NAP1 proteins 673598 N/A 673602 unspecified role unspecified role prey prey N/A 673600 unspecified role unspecified role bait bait physical association physical association false false false 10 A abil1-scar2 Interaction between ABIL1 and SCAR2 proteins 673598 N/A 673603 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 673600 unspecified role unspecified role bait bait physical association physical association false false false 10 B arpc3-scar2 Interaction between ARPC3 and SCAR2 proteins 673598 n/a 673604 unspecified role unspecified role bait bait N/A 673603 unspecified role unspecified role prey prey c-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 11 A arpc3-wasf1 Interaction between ARPC3 and Human WAVE1 proteins 673598 n/a 673604 unspecified role unspecified role bait bait N/A 673605 unspecified role unspecified role prey prey physical association physical association false false false 11 scar2-arpc3-1 Interaction between SCAR2 and ARPC3 proteins 673599 n/a 673604 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 673603 unspecified role unspecified role bait bait c-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 11 C