IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ piccio-2005-1 Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation. human-jurkat Homo sapiens jurkat Human T cell leukemia facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection Piccio L., Vermi W., Boles KS., Fuchs A., Strader CA., Facchetti F., Cella M., Colonna M. mcolonna@pathology.wustl.edu Blood (0006-4971) 2005 cd47_human Leukocyte surface antigen CD47 CD47 MER6 Integrin-associated protein Antigenic surface determinant protein OA3 Protein MER6 protein protein human Homo sapiens Human MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE sirpg_human Signal-regulatory protein gamma SIRPG SIRPB2 Signal-regulatory protein beta-2 CD172 antigen-like family member B protein protein human Homo sapiens Human MPVPASWPHPPGPFLLLTLLLGLTEVAGEEELQMIQPEKLLLVTVGKTATLHCTVTSLLPVGPVLWFRGVGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRISSITPADVGTYYCVKFRKGSPENVEFKSGPGTEMALGAKPSAPVVLGPAARTTPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPTGQSVAYSIRSTARVVLDPWDVRSQVICEVAHVTLQGDPLRGTANLSEAIRVPPTLEVTQQPMRVGNQVNVTCQVRKFYPQSLQLTWSENGNVCQRETASTLTENKDGTYNWTSWFLVNISDQRDDVVLTCQVKHDGQLAVSKRLALEVTVHQKDQSSDATPGPASSLTALLLIAVLLGPIYVPWKQKT sirpg-cd47 Binding of soluble Sirpg with CD47-expressing cells 653132 N/A 653133 unspecified role unspecified role prey prey human-jurkat Homo sapiens jurkat Human T cell leukemia N/A 653134 unspecified role unspecified role bait bait c-terminal d1d2-igg fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false Sirpg-D1D2-IgG was found to bind to T-cells expressing CD47, by flow cytometry. The binding was totally blocked by anti-CD47 mAbs, and partially inhibited by anti-Sirpg mAbs. This demonstrates that Sirpg is a receptor for CD47. Figure 5 sirpg-cd47-1 Conjugation between BW-Sirpg cells and CD47+ Jurkat cells 653132 N/A 653133 unspecified role unspecified role prey prey human-jurkat Homo sapiens jurkat Human T cell leukemia N/A 653134 unspecified role unspecified role bait bait mouse-bw Mus musculus carcinoma cells most probably hepatoma bw mouse carcinoma cells most probably hepatoma physical association physical association false false false Sirpg-expressing BW cells were able to conjugate with CD47-expressing Jurkat cells and this cojugation was partially blocked by anti-Sirp and anti-CD47 antibodies but not by anti-CD18 or anti-CD11a antibodies, suggesting that Sirpg-CD47 interaction significantly contributes to adhesion of T-cells to cells expressing CD47. Figure 6A, 6B.