UniProt UniProt http://www.uniprot.org di_stefano-2004-3 P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation di_stefano-2004-2 P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. human-ecv304 Human umbilical cord, endothelial ECV304 ecv304 Human umbilical cord, endothelial ECV304 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation di_stefano-2004-4 P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation di_stefano-2004-1 P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom fingerprinting peptide massfingerprinting fingerprinting Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation di_stefano-2004-5 P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation di_stefano-2004-6 P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. human-ecv304 Human umbilical cord, endothelial ECV304 ecv304 Human umbilical cord, endothelial ECV304 confocal microscopy confocal microscopy immunostaining immunostaining Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation srcn1_mouse SRC kinase signaling inhibitor 1 p140Cap SNAP-25-interacting protein Srcin1 Kiaa1684 P140 p130Cas-associated protein protein protein mouse Mus musculus Mouse MQPWQCLRRFALAWWERTAEGRARSPREEVGPRDPGGRGEPDPERSSPPMLSADDAEYPREYRTLGGGGGGGSGGRRFSNVGLVHTSERRHTVIAAQSLEALSGLQKADADRKRDAFMDHLKSKYPQHALALRGQQDRMREQVGGWTVDPVCLLSSLCSHLHGDSTPSGAGQPAQQPNYWSFKTRSSRHTQGAQPGLADQAAKLSYASAESLETMSEAELPLGFSRMNRFRQSLPLSRSASQTKLRSPGVLFLQFGEETRRVHITHEVSSLDTLHALIAHMFPQKLTMGMLKSPNTAILIKDEARNVFYELEDVRDIQDRSIIKIYRKEPLYAAFPGSHLTNGDLRREMVYASRESSPTRRLNNLSPASHLASSSPPPGLPSGLPSGLPSGSPSRSRLSYAGGRPPSYAGSPVHHAAERLGGAPTGQGVSPSPSAILERRDVKPDEDLAGKAGGMVLVKGEGLYADPYGLLHEGRLSLAAAAETHSHTRARAACTSGVPCALSAPTPLPRCSPTWRTRCTRRALAALYGDGYGFRLPPSSPQKLADVSAPSGGPPPPHSPYSGPPSRGSPVRQSFRKDSGSSSVFAESPGGKARSTGSASTAGAPPSELFPGPGERSLVGFGPPVPAKDTETRERMEAMEKQIASLTGLVQSALLRGSEPETPSEKVEGSNGAATPSAPVCGSGSKSSGATPVSGPPPPSASSTPAGQPTAVSRLQMQLHLRGLQNSASDLRGQLQQLRNVQLQNQESVRALLKPTEADVSMRVSEAARRQEDPLQRQRTLVEEERLRYLNDEELITQQLNDLEKSVEKIQRDVAHNHRLMPGPELEEKALVLKQLGETLTELKAHFPGLQSKMRVVLRVEVEAVKFLKEEPQRLDGLLKRCRGVTDTLAQIRRQVDEGMWPPPNNLLNQSPKKVAAETDFSKGLDFEIPPPSPPLNLHELSGPAEGTPLTPKSTNPTKCLDASSKRNTDKAVSVEAAERDWEEKRAALTQYSAKDINRLLEETQAELLKAIPDLDCASKTHPGPAPTPDHKPPKAPHGQKAAPRTEPSGRRGSDELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRTGEVVVTSKKDSVFIKKAESEELEVQKPQVKLRRAVSEVVRPASTPPIMASAIKDEDDEERIIAELESGGSSVPPMKVVTPGASRLKAAQGPAGSPDKGKHGKQRTEYMRIQAQQQATKPSKEVSGPNETSSPGSEKPSGSRTSIPVLTSFGARNSSISF bcar1_human Breast cancer anti-estrogen resistance protein 1 CRK-associated substrate p130cas BCAR1 CAS CRKAS CASS1 Cas scaffolding protein family member 1 protein protein human Homo sapiens Human MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGPGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPSPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPAEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVERVKELGHSTQQFRRVLGQLAAA crk_human Adapter molecule crk Proto-oncogene c-Crk CRK p38 protein protein human Homo sapiens Human MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEPGPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWEGECNGKRGHFPFTHVRLLDQQNPDEDFS srcn1_human SRC kinase signaling inhibitor 1 SRCIN1 KIAA1684 P140 p130Cas-associated protein p140Cap SNAP-25-interacting protein SNIP protein protein human Homo sapiens Human MGNAPSQDPERSSPPMLSADDAEYPREYRTLGGGGGGGSGGRRFSNVGLVHTSERRHTVIAAQSLEALSGLQKADADRKRDAFMDHLKSKYPQHALALRGQQDRMREQPNYWSFKTRSSRHTQGAQPGLADQAAKLSYASAESLETMSEAELPLGFSRMNRFRQSLPLSRSASQTKLRSPGVLFLQFGEETRRVHITHEVSSLDTLHALIAHMFPQKLTMGMLKSPNTAILIKDEARNVFYELEDVRDIQDRSIIKIYRKEPLYAAFPGSHLTNGDLRREMVYASRESSPTRRLNNLSPAPHLASGSPPPGLPSGLPSGLQSGSPSRSRLSYAGGRPPSYAGSPVHHAAERLGGAPAAQGVSPSPSAILERRDVKPDEDLASKAGGMVLVKGEGLYADPYGLLHEGRLSLAAAAGDPFAYPGAGGLYKRGSVRSLSTYSAAALQSDLEDSLYKAAGGGGPLYGDGYGFRLPPSSPQKLADVAAPPGGPPPPHSPYSGPPSRGSPVRQSFRKDSGSSSVFAESPGGKTRSAGSASTAGAPPSELFPGPGERSLVGFGPPVPAKDTETRERMEAMEKQIASLTGLVQSALLRGSEPETPSEKIEGSNGAATPSAPCGSGGRSSGATPVSGPPPPSASSTPAGQPTAVSRLQMQLHLRGLQNSASDLRGQLQQLRKLQLQNQESVRALLKRTEAELSMRVSEAARRQEDPLQRQRTLVEEERLRYLNDEELITQQLNDLEKSVEKIQRDVSHNHRLVPGPELEEKALVLKQLGETLTELKAHFPGLQSKMRVVLRVEVEAVKFLKEEPQRLDGLLKRCRGVTDTLAQIRRQVDEGVWPPPNNLLSQSPKKVTAETDFNKSVDFEMPPPSPPLNLHELSGPAEGASLTPKGGNPTKGLDTPGKRSVDKAVSVEAAERDWEEKRAALTQYSAKDINRLLEETQAELLKAIPDLDCASKAHPGPAPTPDHKPPKAPHGQKAAPRTEPSGRRGSDELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRTGEVVVTSKKDSAFIKKAESEELEVQKPQVKLRRAVSEVARPASTPPIMASAIKDEDDEDRIIAELESGGGSVPPMKVVTPGASRLKAAQGQAGSPDKSKHGKQRAEYMRIQAQQQATKPSKEMSGSNETSSPVSEKPSASRTSIPVLTSFGARNSSISF bcar1_mouse Breast cancer anti-estrogen resistance protein 1 Bcar1 Cas Crkas p130cas CRK-associated substrate protein protein mouse Mus musculus Mouse MTVPNVLAKALYDNVAESPDELSFRKGDIMTVLERDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPVGPGPGPPATPPQPQPSLPQGVHAPVPPASQYSPMLPTAYQPQSDNVYLVPTPSKTQQGLYQAPGPNPQFQSPPAKQTSTFSKQTPHHSFPSPATDLYQVPPGPGSPAQDIYQVPPSAGIGHDIYQVPPSLDTRGWEGTKPPAKVVVPTRVGQGYVYEAAQTEQDEYDTPRHLLAPGPQDIYDVPPVRGLLPNQYGQEVYDTPPMAVKGPNGRDPLLDVYDVPPSVEKGLLSSSHHSVYDVPPSVSKDVPDGPLLREETYDVPPAFAKPKPFDPTRHPLILAAPPPDSPAAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGSVVDDGVYAVPPPAEREAPTDGKRLSASSTGSTRSSQSASSLEVVVPGREPLELEVAVESLARLQQGVSTTVAHLLDLVGSASGPGGWRGTSEPQEPPAQDLKAAVAAVHGAVHELLEFARGAVSNATHTSDRTLHAKLSRQLQKMEDVYQTLVVHGQVLDSGRGSPGFTPEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKAPGPGPEGSSSLHPNPTDKASSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLERGNIMRQGKGQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLVPGRTGGLGPSDRQLLLFYLEQCEANLTTLTDAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVDRVKELGHSTQQFRRVLGQLAAA p140-bcar1-2 665744 N/A 665750 unspecified role unspecified role bait bait N/A 665751 unspecified role unspecified role prey prey N/A 665752 unspecified role unspecified role prey prey association association false false false 4B bcar1-p140-3 665744 N/A 665750 unspecified role unspecified role prey prey N/A 665751 unspecified role unspecified role bait bait N/A 665752 unspecified role unspecified role prey prey association association false false false 4B crk-p140-1 665744 N/A 665752 unspecified role unspecified role prey prey N/A 665751 unspecified role unspecified role bait bait physical association physical association false false false 4B bcar1-p140-2 665745 N/A 665753 unspecified role unspecified role prey prey N/A 665751 unspecified role unspecified role bait bait physical association physical association false false false 4A p140-bcar1-1 665745 N/A 665753 unspecified role unspecified role bait bait N/A 665751 unspecified role unspecified role prey prey physical association physical association false false false 4A p140-bcar1-3 665746 N/A 665750 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 665754 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false 5A The C-terminal of SNIP as given in the UnIProt database is longer than stated in the paper, nor can this difference be ascribed to any of the known isoforms. bcar1-p140-6 665746 N/A 665750 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 665754 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 5D bcar1-p140-1 665747 N/A 665753 unspecified role unspecified role prey prey human-ecv304 Human umbilical cord, endothelial ECV304 ecv304 Human umbilical cord, endothelial ECV304 N/A 665754 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1B bcar1-p140-5 665748 N/A 665750 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 665751 unspecified role unspecified role bait bait physical association physical association false false false 5C A P140 deletion construct missing the c-terminus (aa 1006-1250) did not interact. The protein in UniProt is longer at the C-terminus than described in the paper p140-bcar1-7 665749 N/A 665753 unspecified role unspecified role neutral component neutral component N/A 665751 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6 Colocalization at cytoplasmic punctate structures. Upon treatment with PMA colocalization occurs at membrane ruffles. P140 is localized to actin cytoskeleton.