UniProt UniProt http://www.uniprot.org lopreiato-2004-1 Analysis of the interaction between piD261/Bud32, an evolutionarily conserved protein kinase of Saccharomyces cerevisiae, and the Grx4 glutaredoxin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lopreiato R., Facchin S., Sartori G., Arrigoni G., Casonato S., Ruzzene M., Pinna LA., Carignani G. Phosphorylation of GRX4 (0-5 uM) by recombinant His-tagged BUD32 (0.3 uM) was performed at 37 degrees Celsius for 15 min in presence of 0.05 M Tris/HCl pH 7.5, 0.01 M MnCl2 and 25 uM gamma-32P-labelled ATP (specific activity 2000 cpm/pmol). giovanna.carignani@unipd.it Biochem. J. (0264-6021) 2004 Only protein-protein interactions imex curation lopreiato-2004-2 Analysis of the interaction between piD261/Bud32, an evolutionarily conserved protein kinase of Saccharomyces cerevisiae, and the Grx4 glutaredoxin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lopreiato R., Facchin S., Sartori G., Arrigoni G., Casonato S., Ruzzene M., Pinna LA., Carignani G. Phosphorylation of GRX4 (0-5 uM) by recombinant His-tagged BUD32 (0.3 uM) was performed at 37 degrees Celsius for 15 min in presence of 0.05 M Tris/HCl pH 7.5, 0.01 M MnCl2 and 25 uM gamma-32P-labelled ATP (specific activity 2000 cpm/pmol). giovanna.carignani@unipd.it Biochem. J. (0264-6021) 2004 Only protein-protein interactions imex curation lopreiato-2004-3 Analysis of the interaction between piD261/Bud32, an evolutionarily conserved protein kinase of Saccharomyces cerevisiae, and the Grx4 glutaredoxin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lopreiato R., Facchin S., Sartori G., Arrigoni G., Casonato S., Ruzzene M., Pinna LA., Carignani G. Phosphorylation of GRX4 (0-5 uM) by recombinant His-tagged BUD32 (0.3 uM) was performed at 37 degrees Celsius for 15 min in presence of 0.05 M Tris/HCl pH 7.5, 0.01 M MnCl2 and 25 uM gamma-32P-labelled ATP (specific activity 2000 cpm/pmol). giovanna.carignani@unipd.it Biochem. J. (0264-6021) 2004 Only protein-protein interactions imex curation lopreiato-2004-4 Analysis of the interaction between piD261/Bud32, an evolutionarily conserved protein kinase of Saccharomyces cerevisiae, and the Grx4 glutaredoxin. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Lopreiato R., Facchin S., Sartori G., Arrigoni G., Casonato S., Ruzzene M., Pinna LA., Carignani G. Phosphorylation of GRX4 (0-5 uM) by recombinant His-tagged BUD32 (0.3 uM) was performed at 37 degrees Celsius for 15 min in presence of 0.05 M Tris/HCl pH 7.5, 0.01 M MnCl2 and 25 uM gamma-32P-labelled ATP (specific activity 2000 cpm/pmol). giovanna.carignani@unipd.it Biochem. J. (0264-6021) 2004 Only protein-protein interactions imex curation lopreiato-2004-5 Analysis of the interaction between piD261/Bud32, an evolutionarily conserved protein kinase of Saccharomyces cerevisiae, and the Grx4 glutaredoxin. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Lopreiato R., Facchin S., Sartori G., Arrigoni G., Casonato S., Ruzzene M., Pinna LA., Carignani G. Phosphorylation of GRX4 (0-5 uM) by recombinant His-tagged BUD32 (0.3 uM) was performed at 37 degrees Celsius for 15 min in presence of 0.05 M Tris/HCl pH 7.5, 0.01 M MnCl2 and 25 uM gamma-32P-labelled ATP (specific activity 2000 cpm/pmol). giovanna.carignani@unipd.it Biochem. J. (0264-6021) 2004 Only protein-protein interactions imex curation bud32_yeast EKC/KEOPS complex subunit BUD32 YGR262C BUD32 LDB14 piD261 Low-dye-binding protein 14 Atypical serine/threonine protein kinase BUD32 Bud site selection protein 32 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTQEFIDKVSSYLTPDVDIAPISQGAEAIVFTTTTHPYLPRAKDSHQKYIIKYRPPKRYRHPQIDQALTKHRTLNESRLLAKLYLIPGLCVPQLIACDPYNGFIWLEFLGEDLPGGHGFSNLKNFLWMHDQDPYSDLVATTLRKVGRQIGLLHWNDYCHGDLTSSNIVLVRDGARWTPHLIDFGLGSVSNLVEDKGVDLYVLERAILSTHSKHAEKYNAWIMEGFEEVYREQGAKGAKKLKEVTKRFEEVRLRGRKRSMLG D12E97BAE21B3385 glrx4_yeast Monothiol glutaredoxin-4 GRX4 SYGP-ORF64 YER174C protein protein yeast Saccharomyces cerevisiae Baker's yeast MTVVEIKSQDQFTQLTTTNAANKLIVLYFKAQWADPCKTMSQVLEAVSEKVRQEDVRFLSIDADEHPEISDLFEIAAVPYFVFIQNGTIVKEISAADPKEFVKSLEILSNASASLANNAKGPKSTSDEESSGSSDDEEDETEEEINARLVKLVQAAPVMLFMKGSPSEPKCGFSRQLVGILREHQIRFGFFDILRDENVRQSLKKFSDWPTFPQLYINGEFQGGLDIIKESIEEDPEYFQHALQ 86B4C4993545B5B2 glrx3_yeast Monothiol glutaredoxin-3 YD8557.05C GRX3 YDR098C protein protein yeast Saccharomyces cerevisiae Baker's yeast MPVIEINDQEQFTYLTTTAAGDKLIVLYFHTSWAEPCKALKQVFEAISNEPSNSNVSFLSIDADENSEISELFEISAVPYFIIIHKGTILKELSGADPKEYVSLLEDCKNSVNSGSSQTHTMENANVNEGSHNDEDDDDEEEEEETEEQINARLTKLVNAAPVMLFMKGSPSEPKCGFSRQLVGILREHQVRFGFFDILRDESVRQNLKKFSEWPTFPQLYINGEFQGGLDIIKESLEEDPDFLQHALQS 8CC1A04EC1E7DAB3 kae1_yeast tRNA N6-adenosine threonylcarbamoyltransferase KAE1 t(6)A37 threonylcarbamoyladenosine biosynthesis protein KAE1 Kinase-associated endopeptidase 1 tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 YKR038C N6-L-threonylcarbamoyladenine synthase protein protein yeast Saccharomyces cerevisiae Baker's yeast MVNLNTIPPKNGRDYYIALGLEGSANKLGVGIVKHPLLPKHANSDLSYDCEAEMLSNIRDTYVTPPGEGFLPRDTARHHRNWCIRLIKQALAEADIKSPTLDIDVICFTKGPGMGAPLHSVVIAARTCSLLWDVPLVGVNHCIGHIEMGREITKAQNPVVLYVSGGNTQVIAYSEKRYRIFGETLDIAIGNCLDRFARTLKIPNEPSPGYNIEQLAKKAPHKENLVELPYTVKGMDLSMSGILASIDLLAKDLFKGNKKNKILFDKTTGEQKVTVEDLCYSLQENLFAMLVEITERAMAHVNSNQVLIVGGVGCNVRLQEMMAQMCKDRANGQVHATDNRFCIDNGVMIAQAGLLEYRMGGIVKDFSETVVTQKFRTDEVYAAWRD 76A73F2E9E26CF4C imdh1_yeast Putative inosine-5'-monophosphate dehydrogenase 1 IMD1 FUN63 YAR073W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAAIRDYKTALDLTKSLPRPDGLSVQELMDSKIRGGLAYNDFLILPGLVDFASSEVSLQTKLTRNITLNIPLVSSPMDTVTESEMATFMALLDGIGFIHHNCTPEDQADMVRRVKNYENGFINNPIVISPTTTVGEAKSMKEKYGFAGFPVTADGKRNAKLVGAITSRDIQFVEDNSLLVQDVMTKNPVTGAQGITLSEGNEILKKIKKGRLLVVDEKGNLVSMLSRTDLMKNQKYPLASKSANTKQLLWGASIGTMDADKERLRLLVKAGLDVVILDSSQGNSIFQLNMIKWIKETFPDLEIIAGNVVTKEQAANLIAAGADGLRIGMGTGSICITQKVMACGRPQGTAVYNVCEFANQFGVPCMADGGVQKHWSYYYQSFGSWFFYCYDGWYVGRYYRITR FEBC13E46D5D1ECD imdh2_yeast Inosine-5'-monophosphate dehydrogenase 2 IMD2 PUR5 YHR216W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAAIRDYKTALDFTKSLPRPDGLSVQELMDSKIRGGLTYNDFLILPGLVDFASSEVSLQTKLTRNITLNIPLVSSPMDTVTESEMATFMALLGGIGFIHHNCTPEDQADMVRRVKNYENGFINNPIVISPTTTVGEAKSMKEKYGFAGFPVTTDGKRNAKLVGVITSRDIQFVEDNSLLVQDVMTKNPVTGAQGITLSEGNEILKKIKKGRLLVVDEKGNLVSMLSRTDLMKNQNYPLASKSANTKQLLCGASIGTMDADKERLRLLVKAGLDVVILDSSQGNSIFELNMLKWVKESFPGLEVIAGNVVTREQAANLIAAGADGLRIGMGTGSICITQEVMACGRPQGTAVYNVCEFANQFGVPCMADGGVQNIGHITKALALGSSTVMMGGMLAGTTESPGEYFYQDGKRLKAYRGMGSIDAMQKTGTKGNASTSRYFSESDSVLVAQGVSGAVVDKGSIKKFIPYLYNGLQHSCQDIGCRSLTLLKNNVQRGKVRFEFRTASAQLEGGVHNLHSYEKRLHN 7CA3EC11238906B9 imdh4_yeast Inosine-5'-monophosphate dehydrogenase 4 IMD4 YM9958.06C YML056C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAAPLDYKKALEHLKTYSSKDGLSVQELMDSTTRGGLTYNDFLVLPGLVNFPSSAVSLQTKLTKKITLNTPFVSSPMDTVTEADMAIYMALLGGIGFIHHNCTPKEQASMVKKVKMFENGFINSPIVISPTTTVGEVKVMKRKFGFSGFPVTEDGKCPGKLVGLVTSRDIQFLEDDSLVVSEVMTKNPVTGIKGITLKEGNEILKQTKKGKLLIVDDNGNLVSMLSRADLMKNQNYPLASKSATTKQLLCGAAIGTIEADKERLRLLVEAGLDVVILDSSQGNSVFQLNMIKWIKETFPDLEIIAGNVATREQAANLIAAGADGLRIGMGSGSICITQEVMACGRPQGTAVYNVCQFANQFGVPCMADGGVQNIGHITKALALGSSTVMMGGMLAGTTESPGEYFYKDGKRLKAYRGMGSIDAMQKTGNKGNASTSRYFSESDSVLVAQGVSGAVVDKGSIKKFIPYLYNGLQHSCQDIGCESLTSLKENVQNGEVRFEFRTASAQLEGGVHNLHSYEKRLYN A73D1E4EFE8AEAD9 xpo1_yeast Exportin-1 CRM1 KAP124 YGR218W G8514 XPO1 Chromosome region maintenance protein 1 Karyopherin-124 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEGILDFSNDLDIALLDQVVSTFYQGSGVQQKQAQEILTKFQDNPDAWQKADQILQFSTNPQSKFIALSILDKLITRKWKLLPNDHRIGIRNFVVGMIISMCQDDEVFKTQKNLINKSDLTLVQILKQEWPQNWPEFIPELIGSSSSSVNVCENNMIVLKLLSEEVFDFSAEQMTQAKALHLKNSMSKEFEQIFKLCFQVLEQGSSSSLIVATLESLLRYLHWIPYRYIYETNILELLSTKFMTSPDTRAITLKCLTEVSNLKIPQDNDLIKRQTVLFFQNTLQQIATSVMPVTADLKATYANANGNDQSFLQDLAMFLTTYLARNRALLESDESLRELLLNAHQYLIQLSKIEERELFKTTLDYWHNLVADLFYEVQRLPATEMSPLIQLSVGSQAISTGSGALNPEYMKRFPLKKHIYEEICSQLRLVIIENMVRPEEVLVVENDEGEIVREFVKESDTIQLYKSEREVLVYLTHLNVIDTEEIMISKLARQIDGSEWSWHNINTLSWAIGSISGTMSEDTEKRFVVTVIKDLLDLTVKKRGKDNKAVVASDIMYVVGQYPRFLKAHWNFLRTVILKLFEFMHETHEGVQDMACDTFIKIVQKCKYHFVIQQPRESEPFIQTIIRDIQKTTADLQPQQVHTFYKACGIIISEERSVAERNRLLSDLMQLPNMAWDTIVEQSTANPTLLLDSETVKIIANIIKTNVAVCTSMGADFYPQLGHIYYNMLQLYRAVSSMISAQVAAEGLIATKTPKVRGLRTIKKEILKLVETYISKARNLDDVVKVLVEPLLNAVLEDYMNNVPDARDAEVLNCMTTVVEKVGHMIPQGVILILQSVFECTLDMINKDFTEYPEHRVEFYKLLKVINEKSFAAFLELPPAAFKLFVDAICWAFKHNNRDVEVNGLQIALDLVKNIERMGNVPFANEFHKNYFFIFVSETFFVLTDSDHKSGFSKQALLLMKLISLVYDNKISVPLYQEAEVPQGTSNQVYLSQYLANMLSNAFPHLTSEQIASFLSALTKQYKDLVVFKGTLRDFLVQIKEVGGDPTDYLFAEDKENALMEQNRLEREKAAKIGGLLKPSELDD A81833C7F60F5202 vac8_yeast Vacuolar protein 8 VAC8 YEB3 YEL013W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGSCCSCLKDSSDEASVSPIADNEREAVTLLLGYLEDKDQLDFYSGGPLKALTTLVYSDNLNLQRSAALAFAEITEKYVRQVSREVLEPILILLQSQDPQIQVAACAALGNLAVNNENKLLIVEMGGLEPLINQMMGDNVEVQCNAVGCITNLATRDDNKHKIATSGALIPLTKLAKSKHIRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLSSTDPDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPLNEGLIVDAGFLKPLVRLLDYKDSEEIQCHAVSTLRNLAASSEKNRKEFFESGAVEKCKELALDSPVSVQSEISACFAILALADVSKLDLLEANILDALIPMTFSQNQEVSGNAAAALANLCSRVNNYTKIIEAWDRPNEGIRGFLIRFLKSDYATFEHIALWTILQLLESHNDKVEDLVKNDDDIINGVRKMADVTFERLQRSGIDVKNPGSNNNPSSNDNNSNNNDTGSEHQPVEDASLELYNITQQILQFLH B2E75774B47E5933 met10_yeast Sulfite reductase [NADPH] flavoprotein component MET10 YFR030W protein protein yeast Saccharomyces cerevisiae Baker's yeast MPVEFATNPFGEAKNATSLPKYGTPVTAISSVLFNNVDSIFAYKSFSQPDLLHQDLKKWSEKRGNESRGKPFFQELDIRSGAGLAPLGFSHGLKNTTAIVAPGFSLPYFINSLKTVSHDGKFLLNVGALNYDNATGSVTNDYVTALDAASKLKYGVVTPISANEVQSVALLALAIATFSNNSGAINLFDGLNYSKTVLPLVESVPEASILAKLSKVIAPDAAFDDVLDKFNELTGLRLHNFQYFGAQDAETVFITYGSLESELFNSAISGNNSKIGLINVRVPLPFNVAKFVTHVPSTTKQIVVIGQTLDGSSPSFLRSQVSAALFYHGRTSISVSEYIYQPDFIWSPKAVKSIVSSFIPEFTYNADSSFGEGFIYWASDKSINIDVASKLVKALSLEDGKFVSLRTKFDNLANAGTFQAQFVTSKEQIPVSNIDSTKLSVVEDVSLLKHLDVAATVAEQGSIALVSQKAVKDLDLNSVESYVKNLGIPESFLISIAKKNIKLFIIDGETTNDESKLSLFIQAVFWKLAFGLDVAECTNRIWKSIDSGADISAASISEFLTGAFKNFLSEVPLALYTKFSEINIEKKEDEEEPAALPIFVNETSFLPNNSTIEEIPLPETSEISDIAKKLSFKEAYEVENKLRPDLPVKNFVVKVKENRRVTPADYDRYIFHIEFDISGTGMTYDIGEALGIHARNNESLVKEFLTFYGLNESDVVLVPNKDNHHLLETRTVLQAFVENLDIFGKPPKRFYESLIPYASNEEEKKKLEDLVTPAGAVDLKRFQDVEYYTYADIFELFPSVRPSLEELVTIIEPLKRREYSIASSQKVHPNEVHLLIVVVDWVDNKGRKRYGQASKYISDLAVGSELVVSVKPSVMKLPPSPKQPVIMSGLGTGLAPFKAIVEEKLWQKQQGYEIGEVFLYLGSRHKREEYLYGELWEAYKDAGIITHIGAAFSRDQPQKIYIQDRIKENLDELKTAMIDNKGSFYLCGPTWPVPDITQALQDILAKDAEERGIKVDLDAAIEELKEASRYILEVY 924B4047D645E1C3 trk1_yeast High-affinity potassium transport protein J0693 YJL129C TRK1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MHFRRTMSRVPTLASLEIRYKKSFGHKFRDFIALCGHYFAPVKKYIFPSFIAVHYFYTISLTLITSILLYPIKNTRYIDTLFLAAGAVTQGGLNTVDINNLSLYQQIVLYIVCCISTPIAVHSCLAFVRLYWFERYFDGIRDSSRRNFKMRRTKTILERELTARTMTKNRTGTQRTSYPRKQAKTDDFQEKLFSGEMVNRDEQDSVHSDQNSHDISRDSSNNNTNHNGSSGSLDDFVKEDETDDNGEYQENNSYSTVGSSSNTVADESLNQKPKPSSLRFDEPHSKQRPARVPSEKFAKRRGSRDISPADMYRSIMMLQGKHEATAEDEGPPLVIGSPADGTRYKSNVNKLKKATGINGNKIKIRDKGNESNTDQNSVSSEANSTASVSDESSLHTNFGNKVPSLRTNTHRSNSGPIAITDNAETDKKHGPSIQFDITKPPRKISKRVSTFDDLNPKSSVLYRKKASKKYLMKHFPKARRIRQQIKRRLSTGSIEKNSSNNVSDRKPITDMDDDDDDDDNDGDNNEEYFADNESGDEDERVQQSEPHSDSELKSHQQQQEKHQLQQNLHRMYKTKSFDDNRSRAVPMERSRTIDMAEAKDLNELARTPDFQKMVYQNWKAHHRKKPNFRKRGWNNKIFEHGPYASDSDRNYPDNSNTGNSILHYAESILHHDGSHKNGSEEASSDSNENIYSTNGGSDHNGLNNYPTYNDDEEGYYGLHFDTDYDLDPRHDLSKGSGKTYLSWQPTIGRNSNFLGLTRAQKDELGGVEYRAIKLLCTILVVYYVGWHIVAFVMLVPWIILKKHYSEVVRDDGVSPTWWGFWTAMSAFNDLGLTLTPNSMMSFNKAVYPLIVMIWFIIIGNTGFPILLRCIIWIMFKISPDLSQMRESLGFLLDHPRRCFTLLFPKAATWWLLLTLAGLNITDWILFIILDFGSTVVKSLSKGYRVLVGLFQSVSTRTAGFSVVDLSQLHPSIQVSYMLMMYVSVLPLAISIRRTNVYEEQSLGLYGDMGGEPEDTDTEDDGNDEDDDEENESHEGQSSQRSSSNNNNNNNRKKKKKKKTENPNEISTKSFIGAHLRKQLSFDLWFLFLGLFIICICEGDKIKDVQEPNFNIFAILFEIVSAYGTVGLSLGYPDTNQSFSRQFTTLSKLVIIAMLIRGKNRGLPYSLDRAIILPSDRLEHIDHLEGMKLKRQARTNTEDPMTEHFKRSFTDVKHRWGALKRKTTHSRNPKRSSTTL nat10_yeast RNA cytidine acetyltransferase KRE33 18S rRNA cytosine acetyltransferase Killer toxin-resistance protein 33 NAT10 RRA1 Ribosomal RNA cytidine acetyltransferase 1 N1216 N1858 YNL132W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAKKAIDSRIPSLIRNGVQTKQRSIFVIVGDRARNQLPNLHYLMMSADLKMNKSVLWAYKKKLLGFTSHRKKRENKIKKEIKRGTREVNEMDPFESFISNQNIRYVYYKESEKILGNTYGMCILQDFEALTPNLLARTIETVEGGGIVVILLKSMSSLKQLYTMTMDVHARYRTEAHGDVVARFNERFILSLGSNPNCLVVDDELNVLPLSGAKNVKPLPPKEDDELPPKQLELQELKESLEDVQPAGSLVSLSKTVNQAHAILSFIDAISEKTLNFTVALTAGRGRGKSAALGISIAAAVSHGYSNIFVTSPSPENLKTLFEFIFKGFDALGYQEHIDYDIIQSTNPDFNKAIVRVDIKRDHRQTIQYIVPQDHQVLGQAELVVIDEAAAIPLPIVKNLLGPYLVFMASTINGYEGTGRSLSLKLIQQLRNQNNTSGRESTQTAVVSRDNKEKDSHLHSQSRQLREISLDEPIRYAPGDPIEKWLNKLLCLDVTLIKNPRFATRGTPHPSQCNLFVVNRDTLFSYHPVSENFLEKMMALYVSSHYKNSPNDLQLMSDAPAHKLFVLLPPIDPKDGGRIPDPLCVIQIALEGEISKESVRNSLSRGQRAGGDLIPWLISQQFQDEEFASLSGARIVRIATNPEYASMGYGSRAIELLRDYFEGKFTDMSEDVRPKDYSIKRVSDKELAKTNLLKDDVKLRDAKTLPPLLLKLSEQPPHYLHYLGVSYGLTQSLHKFWKNNSFVPVYLRQTANDLTGEHTCVMLNVLEGRESNWLVEFAKDFRKRFLSLLSYDFHKFTAVQALSVIESSKKAQDLSDDEKHDNKELTRTHLDDIFSPFDLKRLDSYSNNLLDYHVIGDMIPMLALLYFGDKMGDSVKLSSVQSAILLAIGLQRKNIDTIAKELNLPSNQTIAMFAKIMRKMSQYFRQLLSQSIEETLPNIKDDAIAEMDGEEIKNYNAAEALDQMEEDLEEAGSEAVQAMREKQKELINSLNLDKYAINDNSEEWAESQKSLEIAAKAKGVVSLKTGKKRTTEKAEDIYRQEMKAMKKPRKSKKAAN 76721ED0867ED618 bud32-grx4 592835 N/A 592840 unspecified role unspecified role bait bait N/A 592841 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 bud32-grx3 592835 N/A 592840 unspecified role unspecified role bait bait N/A 592842 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 bud32-ykr038c 592835 N/A 592840 unspecified role unspecified role bait bait N/A 592843 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 bud32-imd1 592835 N/A 592840 unspecified role unspecified role bait bait N/A 592844 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 bud32-imd2 592835 N/A 592840 unspecified role unspecified role bait bait N/A 592845 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 bud32-imd4 592835 N/A 592840 unspecified role unspecified role bait bait N/A 592846 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 bud32-xpo1 592835 N/A 592847 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 592840 unspecified role unspecified role bait bait physical association physical association false false false Table 1 bud32-vac8 592835 N/A 592840 unspecified role unspecified role bait bait N/A 592848 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 bud32-met10 592835 N/A 592849 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 592840 unspecified role unspecified role bait bait physical association physical association false false false Table 1 bud32-trk1 592835 N/A 592840 unspecified role unspecified role bait bait N/A 592850 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 bud32-kre33 592835 N/A 592840 unspecified role unspecified role bait bait N/A 592851 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 bud32-grx4-1 592836 N/A 592840 unspecified role unspecified role prey prey N/A 592841 unspecified role unspecified role bait bait physical association physical association false false false No figure bud32-grx4-2 592837 N/A 592840 unspecified role unspecified role prey prey N/A 592841 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3 bud32-grx4-3 592838 N/A 592840 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 592841 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 4 bud32-grx4-4 592839 N/A 592840 enzyme enzyme unspecified role unspecified role c-ter his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 592841 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false Fig. 5 & 6 Km=0.3 uM Vmax=63.35 pmol/min/mg Phosphorylation of GRX4 Ser-134