IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sullivan-2003-4 PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma confocal microscopy confocal microscopy predetermined predetermined participant predetermined Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Exp. Cell Res. (0014-4827) 2003 Only protein-protein interactions imex curation sullivan-2003-1 PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Exp. Cell Res. (0014-4827) 2003 Only protein-protein interactions imex curation sullivan-2003-2 PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Exp. Cell Res. (0014-4827) 2003 Only protein-protein interactions imex curation sullivan-2003-3 PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Exp. Cell Res. (0014-4827) 2003 Only protein-protein interactions imex curation sullivan-2003-5 PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells confocal microscopy confocal microscopy predetermined predetermined participant predetermined Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Exp. Cell Res. (0014-4827) 2003 Only protein-protein interactions imex curation cd44_human CD44 antigen Epican CDw44 Phagocytic glycoprotein I Extracellular matrix receptor III HUTCH-I Phagocytic glycoprotein 1 GP90 lymphocyte homing/adhesion receptor Hermes antigen Hyaluronate receptor Heparan sulfate proteoglycan CD44 LHR MDU2 MDU3 MIC4 protein protein human Homo sapiens Human MDKFWWHAAWGLCLVPLSLAQIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATTLMSTSATATETATKRQETWDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRAFDHTKQNQDWTQWNPSHSNPEVLLQTTTRMTDVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTSTIQATPSSTTEETATQKEQWFGNRWHEGYRQTPKEDSHSTTGTAAASAHTSHPMQGRTTPSPEDSSWTDFFNPISHPMGRGHQAGRRMDMDSSHSITLQPTANPNTGLVEDLDRTGPLSMTTQQSNSQSFSTSHEGLEEDKDHPTTSTLTSSNRNDVTGGRRDPNHSEGSTTLLEGYTSHYPHTKESRTFIPVTSAKTGSFGVTAVTVGDSNSNVNRSLSGDQDTFHPSGGSHTTHGSESDGHSHGSQEGGANTTSGPIRTPQIPEWLIILASLLALALILAVCIAVNSRRRCGQKKKLVINSGNGAVEDRKPSGLNGEASKSQEMVHLVNKESSETPDQFMTADETRNLQNVDMKIGV pace1_human Protein-associating with the carboxyl-terminal domain of ezrin SCYL3 PACE1 Ezrin-binding protein PACE-1 SCY1-like protein 3 protein protein human Homo sapiens Human MGSENSALKSYTLREPPFTLPSGLAVYPAVLQDGKFASVFVYKRENEDKVNKAAKHLKTLRHPCLLRFLSCTVEADGIHLVTERVQPLEVALETLSSAEVCAGIYDILLALIFLHDRGHLTHNNVCLSSVFVSEDGHWKLGGMETVCKVSQATPEFLRSIQSIRDPASIPPEEMSPEFTTLPECHGHARDAFSFGTLVESLLTILNEQVSADVLSSFQQTLHSTLLNPIPKCRPALCTLLSHDFFRNDFLEVVNFLKSLTLKSEEEKTEFFKFLLDRVSCLSEELIASRLVPLLLNQLVFAEPVAVKSFLPYLLGPKKDHAQGETPCLLSPALFQSRVIPVLLQLFEVHEEHVRMVLLSHIEAYVEHFTQEQLKKVILPQVLLGLRDTSDSIVAITLHSLAVLVSLLGPEVVVGGERTKIFKRTAPSFTKNTDLSLEDSPMCVVCSHHSQISPILENPFSSIFPKCFFSGSTPINSKKHIQRDYYNTLLQTGDPFSQPIKFPINGLSDVKNTSEDSENFPSSSKKSEEWPDWSEPEEPENQTVNIQIWPREPCDDVKSQCTTLDVEESSWDDCEPSSLDTKVNPGGGITATKPVTSGEQKPIPALLSLTEESMPWKSSLPQKISLVQRGDDADQIEPPKVSSQERPLKVPSELGLGEEFTIQVKKKPVKDPEMDWFADMIPEIKPSAAFLILPELRTEMVPKKDDVSPVMQFSSKFAAAEITEGEAEGWEEEGELNWEDNNW ezri_human Ezrin EZR VIL2 p81 Cytovillin Villin-2 protein protein human Homo sapiens Human MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL e2rsi6_canlf EZR protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLQYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVSEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKETHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDSAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAEKELSDQIQRALQLEEERKRAQEEAERLEADRVAALRAKEELERQAADQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQDNLQEEGTEYTGYSAELSSEGIMDDRNEEKRITEAEKNERVQRQLMTLTSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL cd44-scyl3 PACE-1 colocalizes with CD44 in MDA-MB-231 cells 659565 N/A 659570 unspecified role unspecified role neutral component neutral component N/A 659571 unspecified role unspecified role neutral component neutral component physical association physical association false false false Q8IZE3 gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for the prey. 5 B This colocalization occurred in lamellar ruffles. cd44-scyl3-1 myc-PACE-1 colocalizes with ezrin in MDA-MB-231 cells 659565 N/A 659572 unspecified role unspecified role neutral component neutral component N/A 659571 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Q8IZE3 gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for PACE-1. 5 C This colocalization occurred only in the most distal part of the lamellipodia. Q8IZE3 transfection vil2-scyl3 Ezrin interacts with PACE-1 in a yeast two-hybrid assay 659566 N/A 659572 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 659571 unspecified role unspecified role prey prey physical association physical association false false false The authors show the protein Ezrin as 585 aa in length but the UniProtKb record P23714 is 586 aa in length. PREY gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for the prey. vil2-scyl3-1 Ezrin interacts with PACE-1 in a yeast two-hybrid assay 659567 N/A 659571 unspecified role unspecified role prey prey N/A 659572 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false The authors show the protein Ezrin as 585 aa in length but the UniProtKb record P23714 is 586 aa in length. PREY gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for the prey. 1 B Clone 33 encodes a partial cDNA encompassing the C-terminal and 3' untranslated region of PACE-1. scyl3-ezrin GST-PACE-1 pulls down ezrin from MDCK lysates 659568 N/A 659573 unspecified role unspecified role prey prey canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line N/A 659571 unspecified role unspecified role bait bait ecobb Escherichia coli (strain B / BL21) physical association physical association false false false BAIT gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for the prey. Clone 33 encodes a partial cDNA encompassing the C-terminal and 3' untranslated region of PACE-1. Only GST clone 33 was able to interact with ezrin under stringent conditions of washing with 1% Triton X-100. 1 C cd44-scyl3-2 PACE-1 colocalizes with GFP-ezrin in MCF-7 cells 659569 N/A 659572 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 659571 unspecified role unspecified role neutral component neutral component physical association physical association false false false Q8IZE3 gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for the prey. 5 D P23714; Q8IZE3 transfection