UniProt UniProt http://www.uniprot.org setty-2003-1 Golgi recruitment of GRIP domain proteins by Arf-like GTPase 1 is regulated by Arf-like GTPase 3. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Setty SR., Shin ME., Yoshino A., Marks MS., Burd CG. cburd@mail.med.upenn.edu Curr. Biol. 13 (401-404) 2003 Only protein-protein interactions imex curation arl1_yeast ADP-ribosylation factor-like protein 1 ARL1 Arf-like GTPase 1 ARF3 YBR1216 YBR164C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGNIFSSMFDKLWGSNKELRILILGLDGAGKTTILYRLQIGEVVTTKPTIGFNVETLSYKNLKLNVWDLGGQTSIRPYWRCYYADTAAVIFVVDSTDKDRMSTASKELHLMLQEEELQDAALLVFANKQDQPGALSASEVSKELNLVELKDRSWSIVASSAIKGEGITEGLDWLIDVIKEEQL imh1_yeast Golgin IMH1 Integrins and myosins homology protein 1 YLR309C IMH1 SYS3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFKQLSQIGKNLTDELAKGLADDMSPTPSEQQIEDDKSGLPKEIQAKLRKFEKYEQKYPLLLSAYKNEKLKSEKLEAVEKILAENTPISNIDDAVDTLPAFFQDLNNKNNLLNDEIKRLTKQNSEIPESASSETLKDKEEEFLKKEQNYKNDIDDLKKKMEALNIELDTVQKEKNDTVSGLREKIVALENILKEEREAKKQKEEVSISELKEELAIKNHSLEDSRMKITELEQNLSSKSTIMEEKSSELAELNITLKEKERKLSELEKKMKELPKAISHQNVGNNNRRKKNRNKGKKNKGGITTGDISEEETVDNSINTEEYDKLKENLQELQEKYKDCEDWKQKYEDIEAELKDAKELENSQLEKSAKELETLNTELIDTKKSLKEKNSELEEVRDMLRTVGNELVDAKDEIKESSSKQNEEVKTVKLELDDLRHKNATMIEAYEAKNTELRSKIELLSKKVEHLKNLCTEKEKEQTTSQNKVAKLNEEISQLTYEKSNITKELTSLRTSYKQKEKTVSYLEEQVKQFSEQKDVAEKSTEQLRKDHAKISNRLDLLKKENETLHNDIAKNSNSYEEYLKENGKLSERLNILQEKYNTLQNVKSNSNEHIDSIKRQCEELNVKLKESTKKILSLEDELNEYANIVQDKTREANTLRRLVSDSQTDDSSKQKELENKLAYLTDEKNKLEAELDLQTSRKATELQEWKHTVTELKSEIHALKLREEGLKSEVDALKHVNNDIKRKTQATSDDSDQLEQITSNLKLSLSKADEKNFELQSANEKLLNLNNELNKKFDRLLKNYRSLSSQLNALKERQYSDKSGRVSRSGSIGTLANANIDSSPANNSNPTKLEKIRSSSSLELDSEKNEKIAYIKNVLLGFLEHKEQRNQLLPVISMLLQLDSTDEKRLVMSLK 090BD9AA5702C01D imh1-arl1 677802 N/A 677803 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 677804 unspecified role unspecified role bait bait region GRIP Domain binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3 The interaction needs Arl1 to be in GTP-bound activated form.