UniProt UniProt http://www.uniprot.org saitoh-2002-1 Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap mudpit multidimensional protein identification technology mudpit Saitoh S., Chabes A., McDonald WH., Thelander L., Yates JR., Russell P. prussell@scripps.edu Cell (0092-8674) 2002 saitoh-2002-2 Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Saitoh S., Chabes A., McDonald WH., Thelander L., Yates JR., Russell P. prussell@scripps.edu Cell (0092-8674) 2002 cid13_schpo Poly(A) RNA polymerase cid13 Polynucleotide adenylyltransferase cid13 Caffeine-induced death protein 13 cid13 SPAC821.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDNANCVGGCKFETRSFQYRRRIPYSLGADPLPPVHPLSLKNLVDIDTDLISSQLYELYDSIILNDSGLERRYAFVQKLEQILKKEFPYKNIKTSLFGSTQSLLASNASDIDLCIITDPPQCAPTTCEVSAAFARNGLKKVVCISTAKVPIVKVWDSELQLSCDCNINKTISTLNTRLMRSYVLCDPRVRPLIVMIKYWAKRRCLNDAAEGGTLTSYTISCMVINFLQKRDPPILPSLQMLPHLQDSSTMTDGLDVSFFDDPDLVHGFGDKNEESLGILFVEFFRFFGYLFDYEHFVLSIRHGTFLSKRAKGWQFQLNNFLCVEEPFHTSRNLANTADEITMKGIQLEFRRVFRLLAYNCNVDDACSQFTFPSLTDTSFMDDYVNELQLEIVPGFSHGRDSSDTSCTESPPEPSHFAWAFDPYNATASPYYNQNINSSIDYSSIYSNDVPAIPPNVPYTFVDPYTYACYINNNSYLPPSYMDFYTWYNSPYPKSSHHFDERHGGDRHEKNLSNSRRYSRNKFHKKKQSSGPFQYYPDAFSFTPTDNNSPPSNSSSSEVVSPVSLHSEPVLSTVQAFKS hsp90_schpo Heat shock protein 90 homolog swo1 hsp90 SPAC926.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSNTETFKFEAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRYQSLSDPHALDAEKDLFIRITPDKENKILSIRDTGIGMTKNDLINNLGVIAKSGTKQFMEAAASGADISMIGQFGVGFYSAYLVADKVQVVSKHNDDEQYIWESSAGGSFTVTLDTDGPRLLRGTEIRLFMKEDQLQYLEEKTIKDTVKKHSEFISYPIQLVVTREVEKEVPEEEETEEVKNEEDDKAPKIEEVDDESEKKEKKTKKVKETTTETEELNKTKPIWTRNPSEVTKEEYASFYKSLTNDWEDHLAVKHFSVEGQLEFRAILFVPRRAPMDLFEAKRKKNNIKLYVRRVFITDDCEELIPEWLGFIKGVVDSEDLPLNLSREMLQQNKIMKVIRKNLVRRCLDMFNEIAEDKENFKTFYDAFSKNLKLGIHEDAANRPALAKLLRYNSLNSPDDLISLEDYITKMPEHQKNIYFITGESKQAVENSPFLEIFRAKKFDVLFMVDPIDEYAVTQLKEFEGKKLVNITKDGLELEETDEEKAAREKLEKEYEEFAKQLKTILGDKVEKVVVSNKIVGSPCLLTTGQYGWSANMERIMKAQALRDTSMSAYMSSRKTFEINPKSPIIAELKKKVEENGAEDRSVKDLATILYETALLSSGFTLDDPSAYAQRINRLISLGLSIDEEEEAPIEEISTESVAAENNAESKMEEVD ydmc_schpo Heat shock protein 70 homolog C57A7.12 SPAC57A7.12 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MQLKLTKTLPFSENFIMADSEEYKTVIGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADNSVTNFRDLLGKSHDELTLHHCHYSANPVNVEGQIGFKITVQEDEESDPKEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGLPVLQLIHDPAAVILALMYSEEVLIDKTVVVANFGATRSEVSVVSVKGGLMTILASVHDENLGGEQLTDVLVNFFAKEFEKKNGIDPRKNARSLAKLRAQCEITKRVLSNGTTASAAVDSLADGIDFHSSINRLRYDLAASATLNRMADLVTEAVEKANMEPFDISEVILAGGASNTPKLTSLMESIFPEQTIIRSSSSVTPLQLDPSELTAIGSGVQASLIGHFDAADIAASTDAQVVDVPHLTAPIGINEGENFVTIFDIETALPARKTVEVIAPKEGAAFIPIYEAERSVKVTKVEPEPIDEEEAFSDDEEEEPEEIKERIAIPKTLIATITLPDVSPNAKIELVLQIDAEGKLTASARPKDGKGTNVRGSTA rad25_schpo Checkpoint signal transducer rad25 rad25 SPAC17A2.13c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDAEYNQSAKEEAPAAAAASENEHPEPKESTTDTVKA ye85_schpo Uncharacterized WD repeat-containing protein C9G1.05 SPAC9G1.05 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSQLKSTWAPVPSTKPSQPCKIGTDFKGERIVYPANKAIIIREIEKQENCFQYNEHTAPTTVARFSPSGYYVASGDNQGNVRIWDCAGEDKILKNQVTAISGRITDLDWDGDSQRIIAVGEGKERYGHAFTADSGNSVGEIFGHSSVVNAVSLRKSRPFRAATASDDNSINFFHGTPYRFNRSLRVHSKFVYDVRYSPNDERFASAGADGKVYVFDGKTGDQVYEIDAHKGSIFSISWSPDSSQFVTSSAGYSCKIWDANTGSLIREWLSSDKKQLVGTVWPTKDLIIVVNSKGNLTYLNPSDCKVIDTIYGHQRSITAATLSPDATHFYTASYDGTVLSWDIGKQKAFPLVGESHTNQVMQMIMADDHVITIGMDDTLRVIDIKQGCFAKDNVFPTGYQPIGVCSVEDCLILVTVSDIQVLRSLTGVSTAKTIYQPSAVASHPLKSEFCVGGEDCCVYIHTLEKGELCEVAQCKDSTAPITCLAYSPDGKYLACGDASGKVVLYDANSREVITSRWAFHTGRILGMSWNAKSTHLATASLDTNIHIYSVERPMKYIAMKNAHSLGATQVEWVSENELLSTGSDAAIKVWSVTF rad24_schpo Checkpoint signal transducer rad24 rad24 SPAC8E11.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSTTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDAEYSAAAAGGNTEGAQENAPSNAPEGEAEPKADA pabp_schpo Polyadenylate-binding protein, cytoplasmic and nuclear Polyadenylate tail-binding protein pab1 pabp SPAC57A7.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPSTDLKKQADAAVESDVNTNNEAVESSTKEESSNTPSTETQPEKKAEEPEAAAEPSESTSTPTNASSVATPSGTAPTSASLYVGELDPSVTEAMLFELFNSIGPVASIRVCRDAVTRRSLGYAYVNFHNMEDGEKALDELNYTLIKGRPCRIMWSQRDPSLRKMGTGNVFIKNLDPAIDNKALHDTFSAFGKILSCKVAVDELGNAKGYGFVHFDSVESANAAIEHVNGMLLNDKKVYVGHHVSRRERQSKVEALKANFTNVYIKNLDTEITEQEFSDLFGQFGEITSLSLVKDQNDKPRGFGFVNYANHECAQKAVDELNDKEYKGKKLYVGRAQKKHEREEELRKRYEQMKLEKMNKYQGVNLFIKNLQDEVDDERLKAEFSAFGTITSAKIMTDEQGKSKGFGFVCYTTPEEANKAVTEMNQRMLAGKPLYVALAQRKEVRRSQLEAQIQARNQFRLQQQVAAAAGIPAVQYGATGPLIYGPGGYPIPAAVNGRGMPMVPGHNGPMPMYPGMPTQFPAGGPAPGYPGMNARGPVPAQGRPMMMPGSVPSAGPAEAEAVPAVPGMPERFTAADLAAVPEESRKQVLGELLYPKVFVREEKLSGKITGMLLEMPNSELLELLEDDSALNERVNEAIGVLQEFVDQEPGFTE cid13-rad24-1 681807 N/A 681809 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 681810 unspecified role unspecified role prey prey N/A 681811 unspecified role unspecified role prey prey N/A 681812 unspecified role unspecified role prey prey N/A 681813 unspecified role unspecified role prey prey N/A 681814 unspecified role unspecified role prey prey association association false false false Fig 7H cid13-pab1-1 681808 N/A 681815 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 681809 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 7I