IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ li-2002e-1 Evidence for the direct interaction between calmodulin and the human epidermal growth factor receptor. in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Li H., Villalobo A. Visualisation of interacting molecules achieved by development with streptavidin conjugated to horseradish peroxidase, identity of EGFR further confirmed by western blot. antonio.villalobo@iib.uam.es 2007-06-01: Antonio Villalobo, Instituto de Investigaciones Biomedicas Universidad Autonoma de Madrid, Spain Biochem. J. (0264-6021) 2002 Only protein-protein interactions imex curation li-2002e-2 Evidence for the direct interaction between calmodulin and the human epidermal growth factor receptor. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Li H., Villalobo A. Visualisation of interacting molecules achieved by development with streptavidin conjugated to horseradish peroxidase, identity of EGFR further confirmed by western blot. antonio.villalobo@iib.uam.es 2007-06-01: Antonio Villalobo, Instituto de Investigaciones Biomedicas Universidad Autonoma de Madrid, Spain Biochem. J. (0264-6021) 2002 Only protein-protein interactions imex curation egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA calm_rat Calmodulin Calm1 Calm Cam Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc CaMII protein protein rat Rattus norvegicus (Rat) Rat MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK calm-egfr-4 Interacton between EGFR and calmodulin demonstrated by crosslinking 650825 N/A 650827 unspecified role unspecified role neutral component neutral component human-a_431 Homo sapiens epidermoid carcinoma a_431 Human epidermoid carcinoma N/A 650828 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 2A calm-egfr-5 Interacton between EGFR and calmodulin demonstrated by crosslinking 650825 N/A 650827 unspecified role unspecified role neutral component neutral component mouse-t17 Mus musculus T17 NIH-3T3 fibroblasts expressing human EGFR t17 Mouse NIH3T3 cells expressing human EGFR N/A 650828 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 2B calm-egfr-6 Interacton between EGFR and calmodulin demonstrated by crosslinking 650825 N/A 650827 unspecified role unspecified role neutral component neutral component mouse-t17 Mus musculus T17 NIH-3T3 fibroblasts expressing human EGFR t17 Mouse NIH3T3 cells expressing human EGFR N/A 650828 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3B Complex formation requires calcium calm-egfr-7 Interacton between EGFR and calmodulin demonstrated by crosslinking 650825 N/A 650828 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 650827 unspecified role unspecified role neutral component neutral component human-a_431 Homo sapiens epidermoid carcinoma a_431 Human epidermoid carcinoma physical association physical association false false false Fig. 3A and 4 Interaction requires calcium. calm-egfr-8 Interacton between EGFR and calmodulin demonstrated by Far Western 650826 N/A 650828 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 650827 unspecified role unspecified role prey prey human-a_431 Homo sapiens epidermoid carcinoma a_431 Human epidermoid carcinoma physical association physical association false false false Fig. 5, 6 & 7 Interaction requires calcium A 160kDa and a 190kDa protein were also identified by binding to calmodulin calm-egfr-9 Interacton between EGFR and calmodulin demonstrated by Far Western 650826 N/A 650827 unspecified role unspecified role prey prey human-a_431 Homo sapiens epidermoid carcinoma a_431 Human epidermoid carcinoma N/A 650828 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5, 6 & 7 Interaction requires calcium A 160kDa and a 190kDa protein were also identified by binding to calmodulin