UniProt UniProt http://www.uniprot.org xu-2001a-1 Yeast PalA/AIP1/Alix homolog Rim20p associates with a PEST-like region and is required for its proteolytic cleavage. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Xu W., Mitchell AP. J. Bacteriol. (0021-9193) 2001 xu-2001a-2 Yeast PalA/AIP1/Alix homolog Rim20p associates with a PEST-like region and is required for its proteolytic cleavage. canal Candida albicans (strain SC5314 / ATCC MYA-2876) Yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Xu W., Mitchell AP. J. Bacteriol. (0021-9193) 2001 xu-2001a-3 Yeast PalA/AIP1/Alix homolog Rim20p associates with a PEST-like region and is required for its proteolytic cleavage. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Xu W., Mitchell AP. J. Bacteriol. (0021-9193) 2001 pala_yeast pH-response regulator protein palA/RIM20 Regulator of IME2 protein 20 RIM20 YOR275C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSELLAIPLKRTLEVDFATELSKLIDTTSFQTASFFQSDILKVVDARNNAIAPDISIDGLSALKEYYVILLQLEKKFPNNQIEFTWFQTLSQKSRGTSQYSLQWEKLTIIYNIGCMYSLLALNSNNDAAESLKTSCLYFQNAAGCFKHVLDHQKNLETIPVVDDATLNALTSLMLAQAQECFWFKAVQDKHKDSLIAKLSQQIVDFYCEAINDAQRGKLIRSDWINHLKAKKAYFSAVTYYRIALSFNEKKQFGNVVKALQMGLQFINESTLSSQAKFKTVVESSLKEAQRDNEFIYLQEVPSELPSIKPALMVKPSSSATLLPSIKKDETLFKDLIPIEVMEYCTAYNERQDEYVEQRVTNPLASLNKLLKESLTTFQIPQGLTKVSEAELSHYQASLNNLLINNKNVQVQLDNIEQILNEEAFTDNQLRLKHGTLNWTLPESSTTNTAYYEKLKKLRGYLDEGSAIDKQTNELFQSIDKNLIGSEIRLPESNDPLTNKIKMIIQERNDYIDRTRRKSSEYRILPKIITSYKKNGTVDFEPIFIGHLKYFDEDLRYVNSTKEENIKLIEEVNLSKKNNPGRSGIEPKKMVRIDPRELYIEDLRYSFKLLDEVKENLSAGTAFYENLITSTSNLYNEVQEYDTARRAEKARLDKSLTFEDQ pacc_yeast pH-response transcription factor pacC/RIM101 RIM1 Regulator of IME2 protein 1 YHL027W RIM101 pH-response regulator protein RIM101 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVPLEDLLNKENGTAAPQHSRESIVENGTDVSNVTKKDGLPSPNLSKRSSDCSKRPRIRCTTEAIGLNGQEDERMSPGSTSSSCLPYHSTSHLNTPPYDLLGASAVSPTTSSSSDSSSSSPLAQAHNPAGDDDDADNDGDSEDITLYCKWDNCGMIFNQPELLYNHLCHDHVGRKSHKNLQLNCHWGDCTTKTEKRDHITSHLRVHVPLKPFGCSTCSKKFKRPQDLKKHLKIHLESGGILKRKRGPKWGSKRTSKKNKSCASDAVSSCSASVPSAIAGSFKSHSTSPQILPPLPVGISQHLPSQQQQRAISLNQLCSDELSQYKPVYSPQLSARLQTILPPLYYNNGSTVSQGANSRSMNVYEDGCSNKTIANATQFFTKLSRNMTNNYILQQSGGSTESSSSSGRIPVAQTSYVQPPNAPSYQSVQGGSSISATANTATYVPVRLAKYPTGPSLTEHLPPLHSNTAGGVFNRQSQYAMPHYPSVRAAPSYSSSGCSILPPLQSKIPMLPSRRTMAGGTSLKPNWEFSLNQKSCTNDIIMSKLAIEEVDDESEIEDDFVEMLGIVNIIKDYLLCCVMEDLDDEESEDKDEENAFLQESLEKLSLQNQMGTNSVRILTKYPKILV pala_canal pH-response regulator protein palA/RIM20 RIM20 ENX3 CaO19.4800 CaO19.12263 CAALFM_C109380WA protein protein canal Candida albicans (strain SC5314 / ATCC MYA-2876) Yeast MNTNLLFIPLKQSSVLDLGDELRQVITNNYFQPASSFNSDLIYITQLRNQVAQIKNVNDELGKTSQDDSILLEYLQVLNTLQNKFSDDCVEFAWFDTLAYGPQGPYRYRSLKIEKLNVIYQIGSLYSQIAISESRHTDIGLKRACHYFQLSAGCFMFINNFLIETIKNKNDPLVLSIPLSMQSSTIQCLEYLMLAQAQETIWQKAINNNSMKDSVVARLSIQTSEYYSKALDFGNSSDLIKLEWINHMKVKKLHFLAAAHLRSSTIAADSFQYGEQVAHLRVASQAVDQALKSKKYVNSLVLEDLQGLADVIKVRLRTAEKDNDLVYLKIVPQEKELKTIIGVSMVKSIIPEAFEQKPESNTEIFKELLPYVIIHVAQAMRERQDEYVQTKFHTPISSLNNMLVKFLIDRDLPSSIDSIQQPENIPDSVIHHSQEILKTGGVDFIDKAFKELDKLKLECNHLLQECETRIDLDRSEDDMLRRKQGSTRWTRKSTDDAAQELIKKVDKMKQYLLQANNGDGFVLKKFYDIKPALDVYSSGYKALSQYIPNSQYIELPEQLSIVISDLRSCLARANTLESERKEFLQTLEIRSRDNSILPKLITEYNSNRSKYQTENGEFNFNAFESVYEKHMKLFDSDIKYIARTRDSQMTLEHEIDSINQKFVQEFKSLQNSSNEKRQQVLQYLETAYEKFLEIINNLTEGSKFYTDFIEKGSAVLQECEEYLYRRRVESRDLELAISNSFQQQQQVQEQNEQLSVPRNYSNDKLISPKTSKPGLWNPNSDIQFS pacc_canal pH-response transcription factor pacC/RIM101 RIM101 HRM101 PRR2 CaO19.7247 pH-response regulator protein 2 CAALFM_C114340CA protein protein canal Candida albicans (strain SC5314 / ATCC MYA-2876) Yeast MNYNIHPVTYLNADSNTGASESTASHHGSKKSPSSDIDVDNATSPSSFTSSQSPHINAMGNSPHSSFTSQSAANSPITDAKQHLVKPTTTKPAAFAPSANQSNTTASQSYTQPAQQLPTQLHPSLNQAYNNQPSYYLHQPTYGYQQQQQQQQQHQEFNQPSQQYHDHHGYYSNNNILNQNQPAPQQNPVKPFKKTYKKIRDEDLKGPFKCLWSNCNIIFETPEILYDHLCDDHVGRKSSNNLSLTCLWENCGTTTVKRDHITSHLRVHVPLKPFHCDLCPKSFKRPQDLKKHSKTHAEDHPKKLKKAQRELMKQQQKEAKQQQKLANKRANSMNATTASDLQLNYYSGNPADGLNYDDTSRKRRYENNSQHNMYVVNSILNDFNFQQMAQAPQQPGVVGTAGSAEFTTKRMKAGTEYNIDVFNKLNHLDDHLHHHHPQQQHPQQQYGGNIYEAEKFFNSLSNSIDMQYQNMSTQYQQQHAGSTFAQQKPTQQASGQLYPSLPTIGNGSYTTSGSSHKEGLVNNHNGYLPSYPQINRSLPYSSGVAQQPPSALEFGGVSTYQKSAQSYEEDSSDSSEEDDYSTSSEDELDTLFDKLNIDDNKVEEVTIDGFNLKDVAKHREMIHAVLGYLRNQIEQQEKEKSKEQKEVDVNETKLYPTITAF rim101-rim20-1 655647 N/A 655650 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q12033:p.Gln177_Glu180delinsAlaAlaAlaAla mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q12033:p.Phe623_Tyr624delinsAlaAla mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false N/A 655651 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 3, Table I and 5E rim20-rim101-1 655647 N/A 655650 unspecified role unspecified role bait bait N/A 655651 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3 rim101-rim20-2 655648 N/A 655652 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 655653 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table I rim20-rim101-2 655649 N/A 655650 unspecified role unspecified role bait bait region GAD-Rim20 fusion including a HA-tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 655651 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 4