IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ krogan-2001-1 Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Krogan NJ., Greenblatt JF. jack.greenblatt@utoronto.ca Mol. Cell. Biol. (0270-7306) 2001 Only protein-protein interactions imex curation elp2_yeast Elongator complex protein 2 YGR200C G7725 ELP2 TOT2 Gamma-toxin target 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVECITPEAIFIGANKQTQVSDIHKVKKIVAFGAGKTIALWDPIEPNNKGVYATLKGHEAEVTCVRFVPDSDFMVSASEDHHVKIWKFTDYSHLQCIQTIQHYSKTIVALSALPSLISVGCADGTISIWRQNIQNDEFGLAHEFTIKKGFFYPLCLSLSKVEEKKYLLAIGGTNVNVFIASFILSDSGIEKCRVVAELEGHEDWVKSLAFRHQETPGDYLLCSGSQDRYIRLWRIRINDLIDDSEEDSKKLTLLSNKQYKFQIDDELRVGINFEALIMGHDDWISSLQWHESRLQLLAATADTSLMVWEPDETSGIWVCSLRLGEMSSKGASTATGSSGGFWSCLWFTHERMDFFLTNGKTGSWRMWATKDNIICDQRLGISGATKDVTDIAWSPSGEYLLATSLDQTTRLFAPWIYDASGRKREIATWHEFSRPQIHGYDMICVETVTDTRFVSGGDEKILRSFDLPKGVAGMLQKFVGIQFEEKSEMPDSATVPVLGLSNKAGEDDANEDDEEEEGGNKETPDITDPLSLLECPPMEDQLQRHLLWPEVEKLYGHGFEITCLDISPDQKLIASACRSNNVQNAVIRIFSTENWLEIKPALPFHSLTITRLKFSKDGKFLLSVCRDRKWALWERNMEDNTFELRFKNEKPHTRIIWDADWAPLEFGNVFVTASRDKTVKVWRHQKEPADDYVLEASIKHTKAVTAISIHDSMIREKILISVGLENGEIYLYSYTLGKFELITQLNEDITPADKITRLRWSHLKRNGKLFLGVGSSDLSTRIYSLAYE 5371908FE2E6EC0D elp1_yeast Elongator complex protein 1 IKI3 YLR384C L3502.7 ELP1 TOT1 Protein IKI3 Gamma-toxin target 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVEHDKSGSKRQELRSNMRNLITLNKGKFKPTASTAEGDEDDLSFTLLDSVFDTLSDSITCVLGSTDIGAIEVQQFMKDGSRNVLASFNIQTFDDKLLSFVHFADINQLVFVFEQGDIITATYDPVSLDPAETLIEIMGTIDNGIAAAQWSYDEETLAMVTKDRNVVVLSKLFEPISEYHLEVDDLKISKHVTVGWGKKETQFRGKGARAMEREALASLKASGLVGNQLRDPTMPYMVDTGDVTALDSHEITISWRGDCDYFAVSSVEEVPDEDDETKSIKRRAFRVFSREGQLDSASEPVTGMEHQLSWKPQGSLIASIQRKTDLGEEDSVDVIFFERNGLRHGEFDTRLPLDEKVESVCWNSNSEALAVVLANRIQLWTSKNYHWYLKQELYASDISYVKWHPEKDFTLMFSDAGFINIVDFAYKMAQGPTLEPFDNGTSLVVDGRTVNITPLALANVPPPMYYRDFETPGNVLDVACSFSNEIYAAINKDVLIFAAVPSIEEMKKGKHPSIVCEFPKSEFTSEVDSLRQVAFINDSIVGVLLDTDNLSRIALLDIQDITQPTLITIVEVYDKIVLLRSDFDYNHLVYETRDGTVCQLDAEGQLMEITKFPQLVRDFRVKRVHNTSAEDDDNWSAESSELVAFGITNNGKLFANQVLLASAVTSLEITDSFLLFTTAQHNLQFVHLNSTDFKPLPLVEEGVEDERVRAIERGSILVSVIPSKSSVVLQATRGNLETIYPRIMVLAEVRKNIMAKRYKEAFIVCRTHRINLDILHDYAPELFIENLEVFINQIGRVDYLNLFISCLSEDDVTKTKYKETLYSGISKSFGMEPAPLTEMQIYMKKKMFDPKTSKVNKICDAVLNVLLSNPEYKKKYLQTIITAYASQNPQNLSAALKLISELENSEEKDSCVTYLCFLQDVNVVYKSALSLYDVSLALLVAQKSQMDPREYLPFLQELQDNEPLRRKFLIDDYLGNYEKALEHLSEIDKDGNVSEEVIDYVESHDLYKHGLALYRYDSEKQNVIYNIYAKHLSSNQMYTDAAVAYEMLGKLKEAMGAYQSAKRWREAMSIAVQKFPEEVESVAEELISSLTFEHRYVDAADIQLEYLDNVKEAVALYCKAYRYDIASLVAIKAKKDELLEEVVDPGLGEGFGIIAELLADCKGQINSQLRRLRELRAKKEENPYAFYGQETEQADDVSVAPSETSTQESFFTRYTGKTGGTAKTGASRRTAKNKRREERKRARGKKGTIYEEEYLVQSVGRLIERLNQTKPDAVRVVEGLCRRNMREQAHQIQKNFVEVLDLLKANVKEIYSISEKDRERVNENGEVYYIPEIPVPEIHDFPKSHIVDF 7AEB1C0BA99282F6 elp5_yeast Elongator complex protein 5 YHR187W IKI1 ELP5 HAP2 TOT5 Gamma-toxin target 5 Protein IKI1 HAT-associated protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MASSSHNPVILLKRILSLTESSPFILCLDSIAQTSYKLIQEFVHQSKSKGNEYPIVYISFETVNKPSYCTQFIDATQMDFVHLVKQIISYLPAATATQAKKHMVIIDSLNYISTEYITRFLSEIASPHCTMVATYHKDIKDENRTVIPDWNNNYPDKLTLLQFMATTIVDIDVVLTGTLDTEEVSELLNEFRIPRGLNNDIFQLRLVNKRKSGRSLEYDFIVNSNTHEYELLSTTKQEEESSSNGLETPEMLQGLTTFNLGTSNKQKLAKDQVALPFLEAQSFGQGGAIVYEYEKDDDYDEEDPYEDPF DFD93D7BA583E2F5 elp6_yeast Elongator complex protein 6 YMR312W YM9924.04 ELP6 HAP3 TOT6 Gamma-toxin target 6 HAT-associated protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGSVQRQDLVLFSDQSVLPAHFFQDSNSHNLFFITHQSCTQPLWMINALVETHVLGSPSSLNESSSSMLPSSTRSHAVLASFIHEQNYFTNSLNKLKIPSNNYNVLDFLSDFIVNNIHNKPRDKILSDVLAKFSAAIQNNPTDTIVIIEQPELLLSLVSGLTCSELNNKFITPLLRQCKVLIIVSNSDIFNIDEYDASVHSSNLQNFYKSSFIKSMINLNLNPLKTGFAKDVTGSLHVCRGGAPIATSNTSLHVVENEYLYLNEKESTKLFYR 1A5062465AF67F47 elp4_yeast Elongator complex protein 4 ELP4 Gamma-toxin target 7 HAT-associated protein 1 YPL101W HAP1 TOT7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFRKRGEILNDRGSGLRGPLLRGPPRTSSTPLRTGNRRAPGNVPLSDTTARLKKLNIADESKTKMGLDSSHVGVRPSPATSQPTTSTGSADLDSILGHMGLPLGNSVLVEEQSTTEFHSILGKLFAAQGIVHNRISDSSADKTRNGDTHVIVLSLNQMFAKELPGIYKGSRKQMKKNLISEEESKVTVQNLNETQRSTPSRYKDLKIAWKYKLADEKRLGSPDRDDIQQNSEYKDYNHQFDITTRLMPAPIASELTFIAPTQPVSTILSQIEQTIKRNDKKLIRIVIPSLLHPAMYPPKMFESSEIIGLMHGVRSLVKKYYERVVLFASISIDIITPPLLVLLRNMFDSVINLEPFNQEMTEFLERVYKSQPGKIQHGLVHILKLPVFTDRGEMRVLKSEWAFKNGRKKFEIEQWGIPVDDAEGSAASEQSHSHSHSDEISHNIPAKKTKISLDY 276F0D1538EAA8FA elp3_yeast Elongator complex protein 3 ELP3 Gamma-toxin target 3 HPA1 TOT3 tRNA uridine(34) acetyltransferase YPL086C protein protein yeast Saccharomyces cerevisiae Baker's yeast MARHGKGPKTNKKKLAPEKERFIQCCADITLELTDSLTSGTTREINLNGLITKYSKKYKLKQQPRLTDIINSIPDQYKKYLLPKLKAKPVRTASGIAVVAVMCKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRVEQLKQLGHSIDKVEYVLMGGTFMSLPKEYREDFIVKLHNALSGFNGNDIDEAILYSQQSLTKCVGITIETRPDYCTQTHLDDMLKYGCTRLEIGVQSLYEDVARDTNRGHTVRSVCETFAVSKDAGYKVVSHMMPDLPNVGMERDIEQFKEYFENPDFRTDGLKIYPTLVIRGTGLYELWKTGRYKSYSANALVDLVARILALVPPWTRIYRVQRDIPMPLVTSGVDNGNLRELALARMKDLGTTCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANGGWETFLSYEDPKKDILIGLLRLRKASKKYTYRKEFTSQRTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAERIAKEEHGSEKISVISGVGVRNYYGKLGYELDGPYMSKRI 64EF8B42D3C3102E iki3-elp2-3 Components of the Elongator complex identified by TAP 608794 N/A 608795 unspecified role unspecified role prey prey N/A 608796 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 608797 unspecified role unspecified role prey prey N/A 608798 unspecified role unspecified role prey prey N/A 608799 unspecified role unspecified role prey prey N/A 608800 unspecified role unspecified role prey prey association association false false false Fig. 1 and 2 Rerunning the experiment over a more defined salt gradient, suggested the complex consists of 2 subcomplexes - ELP1, ELP2 and ELP3 and ELP4, ELP5 and ELP6. Isolating the complex is a strain which is ELP2-deficient suggested ELP2 has a role in binding of the smaller subcomplex to Elp1 but is not necessary for effective association of the HAT activity-associated component, Elp3, to the largest subunit of Elongator. iki3-elp2-4 Components of the Elongator complex identified by TAP 608794 N/A 608800 unspecified role unspecified role prey prey N/A 608796 unspecified role unspecified role prey prey N/A 608798 unspecified role unspecified role prey prey N/A 608795 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 608799 unspecified role unspecified role prey prey N/A 608797 unspecified role unspecified role prey prey association association false false false Fig. 1 iki3-elp2-5 Components of the Elongator complex identified by TAP 608794 N/A 608795 unspecified role unspecified role prey prey N/A 608796 unspecified role unspecified role prey prey N/A 608800 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 608797 unspecified role unspecified role prey prey N/A 608799 unspecified role unspecified role prey prey N/A 608798 unspecified role unspecified role prey prey association association false false false Fig. 1 iki3-elp2-6 Components of the Elongator complex identified by TAP 608794 N/A 608799 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 608800 unspecified role unspecified role prey prey N/A 608795 unspecified role unspecified role prey prey N/A 608797 unspecified role unspecified role prey prey N/A 608798 unspecified role unspecified role prey prey N/A 608796 unspecified role unspecified role prey prey association association false false false Fig. 1 iki3-elp2-7 Components of the Elongator complex identified by TAP 608794 N/A 608797 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 608800 unspecified role unspecified role prey prey N/A 608795 unspecified role unspecified role prey prey N/A 608796 unspecified role unspecified role prey prey N/A 608799 unspecified role unspecified role prey prey N/A 608798 unspecified role unspecified role prey prey association association false false false Fig. 1