UniProt UniProt http://www.uniprot.org ochotorena-2001-2 Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ochotorena IL., Hirata D., Kominami K., Potashkin J., Sahin F., Wentz-Hunter K., Gould KL., Sato K., Yoshida Y., Vardy L., Toda T. toda@icrf.icnet.uk An S. pombe genomic cDNA library was used. J. Cell. Sci. (0021-9533) 2001 Only protein-protein interactions imex curation ochotorena-2001-1 Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ochotorena IL., Hirata D., Kominami K., Potashkin J., Sahin F., Wentz-Hunter K., Gould KL., Sato K., Yoshida Y., Vardy L., Toda T. toda@icrf.icnet.uk An S. pombe genomic cDNA library was used. J. Cell. Sci. (0021-9533) 2001 Only protein-protein interactions imex curation ochotorena-2001-3 Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ochotorena IL., Hirata D., Kominami K., Potashkin J., Sahin F., Wentz-Hunter K., Gould KL., Sato K., Yoshida Y., Vardy L., Toda T. toda@icrf.icnet.uk An S. pombe genomic cDNA library was used. J. Cell. Sci. (0021-9533) 2001 Only protein-protein interactions imex curation ochotorena-2001-4 Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Ochotorena IL., Hirata D., Kominami K., Potashkin J., Sahin F., Wentz-Hunter K., Gould KL., Sato K., Yoshida Y., Vardy L., Toda T. toda@icrf.icnet.uk An S. pombe genomic cDNA library was used. J. Cell. Sci. (0021-9533) 2001 Only protein-protein interactions imex curation u2af1_schpo Splicing factor U2AF 23 kDa subunit U2 auxiliary factor 23 kDa subunit U2 snRNP auxiliary factor small subunit SPAP8A3.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MASHLASIYGTEQDKVNCSFYYKIGACRHGERCSRKHVKPNFSQTILCPNMYKNPIHEPNGKKFTQRELAEQFDAFYEDMFCEFSKYGEVEQLVVCDNVGDHLVGNVYVRFKYEESAQNAIDDLNSRWYSQRPVYAELSPVTDFREACCRQHETSECQRGGLCNFMHAKKPSPQLLRDLVLAQRKYLALNAAEEMKKEPNSDSTNRWVSVTAERKN u2af2_schpo Splicing factor U2AF 59 kDa subunit U2 auxiliary factor 59 kDa subunit U2 snRNP auxiliary factor large subunit prp2 mis11 SPBC146.07 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDLSSRLSSGSSRIPKRHRDYRDEEPRRERGSGGIGREDPRGHYGSERPRRRRRDESDFRRHRESRERSYREDERPRRERRYDDYEPRSLRYSSVGRSRSPPPSRERSVRSIEQELEQLRDVTPINQWKRKRSLWDIKPPGYELVTADQAKMSGVFPLPGAPRAAVTDPEKLLEFARSAEGSIIAPPPPLQPGASRQARRLVVTGIPNEFVEDAFVSFIEDLFISTTYHKPETKHFSSVNVCKEENFAILEVATPEDATFLWGLQSESYSNDVFLKFQRIQNYIVPQITPEVSQKRSDDYAKNDVLDSKDKIYISNLPLNLGEDQVVELLKPFGDLLSFQLIKNIADGSSKGFCFCEFKNPSDAEVAISGLDGKDTYGNKLHAQFACVGLNQAMIDKSNGMAILTELAKASSQSIPTRVLQLHNLITGDEIMDVQEYEDIYESVKTQFSNYGPLIDIKIPRSIGTRNSGLGTGKVFVRYSDIRSAEVAMEEMKGCKFNDRTIVIAFYGEDCYKANAW uap2_schpo Splicing factor U2AF-associated protein 2 uap2 SPBC1289.02c Cold sensitive U2 snRNA suppressor 2 homolog protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSQPFWDERIHRWRCLGSEGNELIYIDEEQTWKDYDPNSLKMNKAGSTGAEVSDVTAEATEGKESSNGEDRHTKRLYESTSAEGYPSGSRNKKSKSENSEASPAPVINKAVYIQGLPLDVTVDEIEEVFKKCGVIAKNIDNGTPRIKIYRTEDGTPKGDALIVFFRSESVELAEQLFDDTEFRYGSGQKMRVQKANIDYKKEKTVNKDVGGALKKKALRLRQQQMQQISSWDDVDEEVDDKRKKRFNKIVVLKHIFTLEELDKTPELLIDLKDDITEEAEKCGRVTNVVLYDKEPDGVVTVRFSNNEEAEACVRLMQGRYFDGRVVEASIYDGKVRFQKSGKHTLDDEEDEEKRLEKFADWLENSN wat1_schpo Target of rapamycin complex subunit wat1 pop3 WD repeat-containing protein pop3 wat1 SPBC21B10.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSVQYPPQHSVLLVSSGYDHTIRFWEALSGICSRTIQHADSQVNRLCISPDKKFLAAAGNPHVRLYDINTSSQMPLMTFEGHTNNVTAIAFHCDGKWLATSSEDGTVKVWDMRAPSVQRNYDHKSPVNDLLIHPNQGELLSCDQSGRVRAWDLGENSCTHELIPEEDVPMSSITVGSDGSMLIAGNNKGNCYVWRMLNHQGASLLQPVVKFQAHQRYITRCVLSPDVKHLATCSADATVNIWSTEDMSFMLERRLQGHQRWVWDCAFSADSTYLVTASSDHVARLWELSSGETIRQYSGHHKAAVCVALNDYQI prp2-u2af23-1 682615 N/A 682619 unspecified role unspecified role prey prey N/A 682620 unspecified role unspecified role bait bait physical association physical association false false false Table 2 prp2-uap2-1 682615 N/A 682621 unspecified role unspecified role prey prey N/A 682620 unspecified role unspecified role bait bait physical association physical association false false false Table 2 prp2-pop3-3 682615 N/A 682622 unspecified role unspecified role prey prey N/A 682620 unspecified role unspecified role bait bait physical association physical association false false false Table 2 prp2-pop3-1 682616 N/A 682622 unspecified role unspecified role prey prey N/A 682620 unspecified role unspecified role bait bait physical association physical association false false false pop3-prp2-1 682617 N/A 682622 unspecified role unspecified role bait bait c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 682620 unspecified role unspecified role prey prey physical association physical association false false false Fig 3A prp2-pop3-2 682618 N/A 682622 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 682620 unspecified role unspecified role bait bait physical association physical association false false false Fig 3B