IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ feys-2001-1 Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Feys BJ., Moisan LJ., Newman MA., Parker JE. parker@mpiz-koeln.mpg.de EMBO J. (0261-4189) 2001 feys-2001-2 Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Feys BJ., Moisan LJ., Newman MA., Parker JE. parker@mpiz-koeln.mpg.de Arabidopsis cDNA library made from Peronospora parasitica pathogen challenged plants. EMBO J. (0261-4189) 2001 feys-2001-3 Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Feys BJ., Moisan LJ., Newman MA., Parker JE. A null mutant arabidopsis pad4 line was stably transformed with wild-type PAD4 containing an N-terminal c-Myc epitope tag expressed from the native PAD4 promoter (Myc::PAD4). parker@mpiz-koeln.mpg.de EMBO J. (0261-4189) 2001 eds1c_arath Protein EDS1 EDS1 EDS1-90 EDS1A Enhanced disease susceptibility 1 T17F15.40 At3g48090 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSSFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQASDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQAALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNSGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWITLPKNHKSHSPLRDYMMDEITDT pad4_arath Lipase-like PAD4 PAD4 EDS9 Protein ENHANCED DISEASE SUSCEPTIBILITY 9 Protein PHYTOALEXIN DEFICIENT 4 F22O6.190 At3g52430 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDDCRFETSELQASVMISTPLFTDSWSSCNTANCNGSIKIHDIAGITYVAIPAVSMIQLGNLVGLPVTGDVLFPGLSSDEPLPMVDAAILKLFLQLKIKEGLELELLGKKLVVITGHSTGGALAAFTALWLLSQSSPPSFRVFCITFGSPLLGNQSLSTSISRSRLAHNFCHVVSIHDLVPRSSNEQFWPFGTYLFCSDKGGVCLDNAGSVRLMFNILNTTATQNTEEHQRYGHYVFTLSHMFLKSRSFLGGSIPDNSYQAGVALAVEALGFSNDDTSGVLVKECIETATRIVRAPILRSAELANELASVLPARLEIQWYKDRCDASEEQLGYYDFFKRYSLKRDFKVNMSRIRLAKFWDTVIKMVETNELPFDFHLGKKWIYASQFYQLLAEPLDIANFYKNRDIKTGGHYLEGNRPKRYEVIDKWQKGVKVPEECVRSRYASTTQDTCFWAKLEQAKEWLDEARKESSDPQRRSLLREKIVPFESYANTLVTKKEVSLDVKAKNSSYSVWEANLKEFKCKMGYENEIEMVVDESDAMET pad4-eds1 Yeast 2 hybrid analysis reveals interaction between PAD4 protein and the EDS1 protein 663804 N/A 663807 unspecified role unspecified role prey prey N/A 663808 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2 eds1-eds1 Yeast 2 hybrid analysis reveals homotypic interaction of EDS1 protein 663804 N/A 663807 unspecified role unspecified role prey prey N/A 663807 unspecified role unspecified role bait bait Q9SU72:p.Glu466Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2 eds1-pad4 Yeast 2 hybrid analysis reveals interaction between EDS1 protein and the PAD4 protein 663805 N/A 663808 unspecified role unspecified role prey prey N/A 663807 unspecified role unspecified role bait bait physical association physical association false false false pad4-eds1-1 Yeast 2 hybrid analysis reveals interaction between PAD4 protein and the EDS1 protein 663805 N/A 663808 unspecified role unspecified role bait bait N/A 663807 unspecified role unspecified role prey prey physical association physical association false false false eds1-pad4-1 Coip analysis reveals interaction between EDS1 protein and the PAD4 protein 663806 N/A 663807 unspecified role unspecified role bait bait N/A 663808 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 4 The authors state that after 6 days of exposure to a virulent P.parasitica isolate, Emwa1, enhanced levels of both proteins correlated with increased detection of co-immunoprecipitable PAD4. Also in cases where leaves were sprayed with the bioactive SA analogue, benzothiadiazole (BTH), elevated EDS1 and PAD4 expression corresponded with higher levels of co-immunoprecipitable PAD4 24 h after treatment. Finally in leaves of mature plants were infiltrated with the virulent P.syringae pv. tomato strain, DC3000, or with avirulent DC3000 expressing avrRps4 (DC3000/avrRps4), enhanced levels of EDS1 and PAD4 protein were observed in tissues responding to DC3000/avrRps4 12 h after infiltration and these persisted for up to 48 h 6 days of exposure to a virulent P.parasitica isolate 24 hours after exposure to benzothiadiazole (BTH) 12 hours exposure to virulent P.syringae pv. tomato strain, DC3000 12 hours exposure to avirulent DC3000 expressing avrRps4 (DC3000/avrRps4)