UniProt UniProt http://www.uniprot.org huang-2000-1 Isolation and cloning of four subunits of a fission yeast TFIIIC complex that includes an ortholog of the human regulatory protein TFIIICbeta. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Huang Y., Hamada M., Maraia RJ. maraiar@mail.nih.gov J. Biol. Chem. (0021-9258) 2000 sfc1_schpo Transcription factor tau subunit sfc1 TFIIIC subunit sfc1 Transcription factor C subunit 1 sfc1 tfc1 SPAC6F12.11c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNSLKISDNEYALIEHPGFANNKDAFFQTLGGVQSIQKACQTSFQNPKQALLELNLRPKDKYHHPVQARVQSRNDLLVTIKKMDNSVQNVSRIRQVFLFRDMADFQYSTQNSPFVQKLDSTLRVLDYNAINKFSIDLTPVQRKHVDMPPPPVFSQTSLPMSYNFLQNPLVGRVRLPNGKTTIVNLKGQCRVWIITTNMGVESVPTCRHSKLGEPSKTIQEVIEALKPLFEKRPVWTRRALLNHLDPSYTHYLKFALPYLSYLWTSGPFRDTYTRFGYDPRKDSNAAAYQALFFKLKLNGKHKGTKTHVFDGKTLFPTNRVYQVCDIVDPTIAPLLKDTQLRSECHRDTGWYRSGRYYKVRDLMREKLFALIEGEMPSEVAVNMILNAEEVEESDRYSNFDEQDNTDLNDTVRGLNTSATDDRINDLMRNLMKRSQEHEGFEDLEEIDDDYDDIFGD sfc4_schpo Transcription factor tau subunit sfc4 TFIIIC subunit sfc4 Transcription factor C subunit 4 sfc4 tfc4 SPCC16C4.14c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MIQNGGNSYVDSNMNETQNDTTDNFDAEMQDLNGYISEIVDEARNVSEVDAKFLGDTSALQAEGLWSDEESDYEGSDDESNFSKTASRTEDDIANEEWEENLKAVAGFRKVRKGHKGRGRVSRADMLPSVEVQQMLSLANHLFAQEGNFDEAQKLAEEIVRIDNNVIAAWKMLGECHRQRGNGRVNIEKCLIAWMAAAHLKPKDHELWFTCAKLSESLEFWDQADYCYNRAVSAKPPNKSELKKYIWNRSVLNKEHGSLKKAAEGFKFLLQSSPYNASILKNLAEIYIKIHAPREILKQFEIAWKYFYQYPAPPIGNDIFDLPTLNLYAELLLLDHQWSNLIRLINRGVRWFRGRKSESFWDEFDDDREWDVDERRREFPNASEEHTNKEAYLLPHLFRTKLGIARLKTGELPEAELHFSVIKNLPPDYAWGMLYDIAKAYMDIERLDLALEYFVLICNHEPAQNIGLWYNMGVCYLELKEYEHAQQCMEAILIVDNSNTNALIKLAEINELQDNRDAALEIVTNIFEQRRNINELEREQSQNEDHEKNVGSQLFVGNQKVPQDKWEKRARISRSKEEARQFTIWKTEETQRRFHKLDILRQSLKKEENVSESLNEWLAIASELIDEFVSIKAFFPSEKKARARAGLLTRRTRYASLNDQLTSMINRLNDSLTRTKYGDLDLDTILRTGYFRNVSIDAWYQLFVEFSLRLTKVGSVQQAYDVLTTAMGAILFDQDTIKRQNLRWCMLACSMYARDPQGALTPLRWVFTTFQFRQDTYRLFSAVLSQGYECSRAFVDSANQKFLLRLIKLMDQLMSNSLVSGAATLVKNDDGLATVPTSYDPVLVLLYGHIMARNRSWIPAINYYSRAFAINPDCPITNLSLGLAYLHRAMQRLSDNRHYQILQGFTFLYRYYDLRVNEGLGEKQEALYNLGKAYHFIGLEHYAVKYYEAVLGLSPMSQGDKMTSSESTVSTTYDFGFEAAYNLRLIYICSGNIKLAFQISSKYLIF sfc6_schpo Transcription factor tau subunit sfc6 TFIIIC subunit sfc6 Transcription factor C subunit 6 sfc6 tfc6 SPBC21H7.05 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGPKSKEYENPSSYKNDEDNDDDGDFVLENVMSEEDIEIETPSRNRKRVSTTRRTPSKPIRSQPLTPSSSKGAGNEPKSQNSSTTRGSAKKQSSKGLEEKLINSYGTHVESLNKGRRLIEIWKYYETAPGQSSFEGQTSSDSKLQTLLNLDGQQLLSYSENPFYLFEVKNLSISFHFESPQQVEPCQPVNPFKENPQKSGFVVNTGIPLSSVSWLPTNKETQFLAVGGMLKFSETTESVFMRTSGRNQIQLWKLENKTNFKSEFILYHDWGSVLQLEWCPTISVEDSILGFLAVVCSDGKLRVLRVPRSPVKFHVFVEQADFTFGFNDSLISCCTWVSPEHGDIHQILVGCSNGYLALWDILSSQECPLFYIPYHDSYIHNVVQCLDDFPWLFLTTAFDCYTRIFDIRDPIIDNRPLSHKRDICYTITWNNMLQSIISCSESQSVVIESLRGTSTQLLDERNGSIISLSNSKFHPFVACAASDGIVTIVNPFRLLGFSHKQKANVHRIFQLEYSEKQDSYRMLDGFRPRLPKAKKLDMYIYPWQIQVNKVEWNGNKGYAGWLASGMACGILRVEDLSAVERR sfc3_schpo Transcription factor tau subunit sfc3 sfc3 tfc3 SPBC336.07 TFIIIC subunit sfc3 Transcription factor C subunit 3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDSLIRHCSEEIALDGSSGCSIDRLWEFAANFFFRQGIVQNLDDNYKTFVWPLILKEDGIEVWIEDEDATGLRSKMQEIPWNYQDITIELRARIRLFASEDRQWLTLTYKTKTDSKIQPLAFELLSCISRYRQEGVDRIQLCKETKQEPRSVYGRIQALEDASLISKVAINRSRAQTALLVLKRFESENSTINETVAQVSGKQIYNTEKIRSNICDAVQSSRNGICRHVDARAMVNLNHSRWERRYYARQVTYLHNNGYLRKCLTFVPNSPERCIRCLQFIKPYISSLDATEDDVDFTEVDDIPEDDELEEEEPLLPSKEEFDASFLQPLADVGPTLPQWSRFRPLEFQCFVLIRNAGFHGVITLQILSGLTGIRFNKPLFKLLGSLVEHRSSVPNHLSHMSITRFEDKTKKSRQYRYFTLQSQLNRLIRDGIPAETISKLLPHVHSLAGEFSPIDSSLFLNSLHHKGNMESNAEVSPDGMTLLPRKRGRPRKSANISVTSSPIRPSKNENNLPSLAISPVSEGFIQNATISTPSTSSNLSIAGSLTPSKTSRIYRGLAPLKNPEFNEDHVKKAEHLEPLLNVSSSVPSKNFTVSSPDHLKYGNTSSLQVSDSQSSIDLDSSFHYPVSVDSQLSHSTGSLMANFSSPTKKRRLESVDFIFLQRKGLILSYLVEQNGAFEISRKMFEDLADLKVRRNPETSRTVMDRRTFQQTLEKLMQEKKVRKLVIATNNGLGKLVRKDIVVQYDMKPDSPRFQQLRAQITTPEIEKQSTPEILKDVDVDFLKRNTSLSRRKSMPAEIKRHKESSETKPVDKEEVKKNEKEKDDPMRLAQQLLESLAPDFALHENTQQKSPVEKPKKLRKDRYASVEEFDYFSSTEHASKRSVKRFKNDFTSDEDETLIRAVVITQIYYGGTNRLIKWEAVQKCFPNRDIYALTRRYLSIRQHTKFKGLQQFLSENWQQMYKDAVSRKDLMPYPDSVDDFDPTPYVKASCRPYMISSSNLATTRLPRDLVDVYETYNIEVVKQETNFREMIFDPSLSVASKMNAYCDMPFTMPLSLNDKQNNEGDENCEKGQLFDAKSTIKSIVAIPDATYDARFSQERLMQYPEDILIAAHQELLDKRIITRVNSENSRLQPGRNFQFTEKFASSLKSPLPPFLLSQAKRFNKFLLEGFQNHKNHLFEETSNSGTLACILDLLSQGKLQISIVGSKFNEYGLSEGYRTRLLEHDNINVTLVLSGKESESKKNYGVTEKLATPPSHEPRLWLNGKCELIEMIWMNIEQSIVYQLLRKPGILRSQLTNLLFPGLEPREFNEVLDYFIAAGAAIEKDGLYLNHNYLFKLT sfc3-sfc1-1 682663 N/A 682664 unspecified role unspecified role prey prey N/A 682665 unspecified role unspecified role prey prey N/A 682666 unspecified role unspecified role prey prey N/A 682667 unspecified role unspecified role bait bait n-terminus flag-his tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false Fig 3A, 3B, 3C, 3E