IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chabes-2000-3 Yeast ribonucleotide reductase has a heterodimeric iron-radical-containing subunit. in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation predetermined predetermined participant predetermined Chabes A., Domkin V., Larsson G., Liu A., Graslund A., Wijmenga S., Thelander L. Stoichiometry of interactors taken from relative densities of SDS gel band Andrei.Chabes@medchem.umu.se Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 Only protein-protein interactions imex curation chabes-2000-1 Yeast ribonucleotide reductase has a heterodimeric iron-radical-containing subunit. ecoli Escherichia coli (strain K12) pull down pull down weight by staining molecular weight estimation by staining weight by staining Chabes A., Domkin V., Larsson G., Liu A., Graslund A., Wijmenga S., Thelander L. Stoichiometry of interactors taken from relative densities of SDS gel band Andrei.Chabes@medchem.umu.se Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 Only protein-protein interactions imex curation chabes-2000-2 Yeast ribonucleotide reductase has a heterodimeric iron-radical-containing subunit. ecoli Escherichia coli (strain K12) cd circular dichroism CD CD cd predetermined predetermined participant predetermined Chabes A., Domkin V., Larsson G., Liu A., Graslund A., Wijmenga S., Thelander L. Stoichiometry of interactors taken from relative densities of SDS gel band Andrei.Chabes@medchem.umu.se Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 Only protein-protein interactions imex curation rir2_yeast Ribonucleoside-diphosphate reductase small chain 1 RNR2 YJL026W J1271 CRT6 Ribonucleotide reductase small subunit 1 Ribonucleotide reductase R2 subunit 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPKETPSKAAADALSDLEIKDSKSNLNKELETLREENRVKSDMLKEKLSKDAENHKAYLKSHQVHRHKLKEMEKEEPLLNEDKERTVLFPIKYHEIWQAYKRAEASFWTAEEIDLSKDIHDWNNRMNENERFFISRVLAFFAASDGIVNENLVENFSTEVQIPEAKSFYGFQIMIENIHSETYSLLIDTYIKDPKESEFLFNAIHTIPEIGEKAEWALRWIQDADALFGERLVAFASIEGVFFSGSFASIFWLKKRGMMPGLTFSNELICRDEGLHTDFACLLFAHLKNKPDPAIVEKIVTEAVEIEQRYFLDALPVALLGMNADLMNQYVEFVADRLLVAFGNKKYYKVENPFDFMENISLAGKTNFFEKRVSDYQKAGVMSKSTKQEAGAFTFNEDF DB98028408292042 rir4_yeast Ribonucleoside-diphosphate reductase small chain 2 RNR4 YGR180C CRT3 Ribonucleotide reductase small subunit 2 Ribonucleotide reductase R2 subunit 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEAHNQFLKTFQKERHDMKEAEKDEILLMENSRRFVMFPIKYHEIWAAYKKVEASFWTAEEIELAKDTEDFQKLTDDQKTYIGNLLALSISSDNLVNKYLIENFSAQLQNPEGKSFYGFQIMMENIYSEVYSMMVDAFFKDPKNIPLFKEIANLPEVKHKAAFIERWISNDDSLYAERLVAFAAKEGIFQAGNYASMFWLTDKKIMPGLAMANRNICRDRGAYTDFSCLLFAHLRTKPNPKIIEKIITEAVEIEKEYYSNSLPVEKFGMDLKSIHTYIEFVADGLLQGFGNEKYYNAVNPFEFMEDVATAGKTTFFEKKVSDYQKASDMSKSATPSKEINFDDDF 712853F2D87D3972 rir1_yeast Ribonucleoside-diphosphate reductase large chain 1 RNR1 RIR1 Ribonucleotide reductase R1 subunit 1 YER070W CRT7 SDS12 Ribonucleotide reductase large subunit 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MYVYKRDGRKEPVQFDKITARISRLCYGLDPKHIDAVKVTQRIISGVYEGVTTIELDNLAAETCAYMTTVHPDYATLAARIAISNLHKQTTKQFSKVVEDLYRYVNAATGKPAPMISDDVYNIVMENKDKLNSAIVYDRDFQYSYFGFKTLERSYLLRINGQVAERPQHLIMRVALGIHGRDIEAALETYNLMSLKYFTHASPTLFNAGTPKPQMSSCFLVAMKEDSIEGIYDTLKECALISKTAGGIGLHIHNIRSTGSYIAGTNGTSNGLIPMIRVFNNTARYVDQGGNKRPGAFALYLEPWHADIFDFIDIRKNHGKEEIRARDLFPALWIPDLFMKRVEENGTWTLFSPTSAPGLSDCYGDEFEALYTRYEKEGRGKTIKAQKLWYSILEAQTETGTPFVVYKDACNRKSNQKNLGVIKSSNLCCEIVEYSAPDETAVCNLASVALPAFIETSEDGKTSTYNFKKLHEIAKVVTRNLNRVIDRNYYPVEEARKSNMRHRPIALGVQGLADTFMLLRLPFDSEEARLLNIQIFETIYHASMEASCELAQKDGPYETFQGSPASQGILQFDMWDQKPYGMWDWDTLRKDIMKHGVRNSLTMAPMPTASTSQILGYNECFEPVTSNMYSRRVLSGEFQVVNPYLLRDLVDLGIWDEGMKQYLITQNGSIQGLPNVPQELKDLYKTVWEISQKTIINMAADRSVYIDQSHSLNLFLRAPTMGKLTSMHFYGWKKGLKTGMYYLRTQAASAAIQFTIDQKIADQATENVADISNLKRPSYMPSSASYAASDFVPAAVTANATIPSLDSSSEASREASPAPTGSHSLTKGMAELNVQESKVEVPEVPAPTKNEEKAAPIVDDEETEFDIYNSKVIACAIDNPEACEMCSG 56BE1B077916E419 rnr2-rnr4-4 Interaction between rnr2 and rnr4 identified by density gradient centrifugation 666704 N/A 666707 unspecified role unspecified role neutral component neutral component n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 N/A 666708 unspecified role unspecified role neutral component neutral component ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 physical association physical association false false false Figure 4 Sedimentation coefficient = 5.6S rnr2-1 Homdimerization of rnr2 dentified by density gradient centrifugation 666704 N/A 666707 unspecified role unspecified role neutral component neutral component n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 2.0 direct interaction direct interaction false false false Figure 4 Sedimentation coefficient = 5.6S rnr4-1 Homodimerization of rnr4 identified by density gradient centrifugation 666704 N/A 666708 unspecified role unspecified role neutral component neutral component ecobb Escherichia coli (strain B / BL21) Stoichiometry: 2.0 direct interaction direct interaction false false false Figure 4 Sedimentation coefficient = 5.6S rnr2-rnr4-5 Interaction between rnr2 and rnr4 identified by density gradient centrifugation 666704 N/A 666707 unspecified role unspecified role neutral component neutral component n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 N/A 666708 unspecified role unspecified role neutral component neutral component ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 N/A 666709 unspecified role unspecified role neutral component neutral component ecobb Escherichia coli (strain B / BL21) Stoichiometry: 2.0 physical association physical association false false false Figure 4 Sedimentation coefficient = 9.7S rnr2-rnr4-2 Interaction between rnr2 and rnr4 identified by pulldown 666705 N/A 666708 unspecified role unspecified role prey prey Stoichiometry: 1.0 N/A 666707 unspecified role unspecified role bait bait n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Stoichiometry: 1.0 physical association physical association false false false Figure 1 rnr2-rnr4-3 Interaction between rnr2 and rnr4 identified by CD 666706 N/A 666708 unspecified role unspecified role neutral component neutral component N/A 666707 unspecified role unspecified role neutral component neutral component n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Figure 3 H6Rnr2p gains alpha-helical content on complexing with Rnr4p