UniProt UniProt http://www.uniprot.org kwon-1999-2 Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. caeel Caenorhabditis elegans ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom western blot western blot Immuno blot Immuno blot Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. yjkim@smc.samsung.co.kr Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 Only protein-protein interactions imex curation leej@yonsei.ac.kr kwon-1999-3 Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. Proteins were purified by heparin chromatography prior to immunoprecipitation. yjkim@smc.samsung.co.kr Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 Only protein-protein interactions imex curation leej@yonsei.ac.kr kwon-1999-1 Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. yjkim@smc.samsung.co.kr Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 Only protein-protein interactions imex curation leej@yonsei.ac.kr kwon-1999-4 Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. caeel Caenorhabditis elegans molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. A Superose-6 column was used. yjkim@smc.samsung.co.kr Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 Only protein-protein interactions imex curation leej@yonsei.ac.kr rpb1_caeel DNA-directed RNA polymerase II subunit RPB1 DNA-directed RNA polymerase III largest subunit ama-1 F36A4.7 rpb-1 protein protein caeel Caenorhabditis elegans MALVGVDFQAPLRIVSRVQFGILGPEEIKRMSVAHVEFPEVYENGKPKLGGLMDPRQGVIDRRGRCMTCAGNLTDCPGHFGHLELAKPVFHIGFLTKTLKILRCVCFYCGRLLIDKSAPRVLEILKKTGTNSKKRLTMIYDLCKAKSVCEGAAEKEEGMPDDPDDPMNDGKKVAGGCGRYQPSYRRVGIDINAEWKKNVNEDTQERKIMLTAERVLEVFQQITDEDILVIGMDPQFARPEWMICTVLPVPPLAVRPAVVTFGSAKNQDDLTHKLSDIIKTNQQLQRNEANGAAAHVLTDDVRLLQFHVATLVDNCIPGLPTATQKGGRPLKSIKQRLKGKEGRIRGNLMGKRVDFSARTVITADPNLPIDTVGVPRTIAQNLTFPEIVTPFNVDKLQELVNRGDTQYPGAKYIIRENGARVDLRYHPRAADLHLQPGYRVERHMKDGDIIVFNRQPTLHKMSMMGHRVKILPWSTFRMNLSVTSPYNADFDGDEMNLHLPQSLETRAEIEEIAMVPRQLITPQANKPVMGIVQDTLCAVRMMTKRDVFIDWPFMMDLLMYLPTWDGKVPQPAILKPKPLWTGKQVFSLIIPGNVNVLRTHSTHPDSEDSGPYKWISPGDTKVIIEHGELLSGIVCSKTVGKSAGNLLHVVTLELGYEIAANFYSHIQTVINAWLIREGHTIGIGDTIADQATYLDIQNTIRKAKQDVVDVIEKAHNDDLEPTPGNTLRQTFENKVNQILNDARDRTGSSAQKSLSEFNNFKSMVVSGSKGSKINISQVIACVGQQNVEGKRIPFGFRHRTLPHFIKDDYGPESKGFVENSYLAGLTPSEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSLAQMVQLRYGEDGLDGMWVENQNMPTMKPNNAVFERDFRMDLTDNKFLRKNYSEDVVREIQESEDGISLVESEWSQLEEDRRLLRKIFPRGDAKIVLPCNLQRLIWNAQKIFKVDLRKPVNLSPLHVISGVRELSKKLIIVSGNDEISKQAQYNATLLMNILLRSTLCTKNMCTKSKLNSEAFDWLLGEIESRFQQAIAQPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKEIINVSKTLKTPSLTVFLTGAAAKDPEKAKDVLCKLEHTTLKKVTCNTAIYYDPDPKNTVIAEDEEWVSIFYEMPDHDLSRTSPWLLRIELDRKRMVDKKLTMEMIADRIHGGFGNDVHTIYTDDNAEKLVFRLRIAGEDKGEAQEEQVDKMEDDVFLRCIEANMLSDLTLQGIPAISKVYMNQPNTDDKKRIIITPEGGFKSVADWILETDGTALLRVLSERQIDPVRTTSNDICEIFEVLGIEAVRKAIEREMDNVISFDGSYVNYRHLALLCDVMTAKGHLMAITRHGINRQEVGALMRCSFEETVDILMEAAVHAEEDPVKGVSENIMLGQLARCGTGCFDLVLDVEKCKYGMEIPQNVVMGGGFYGSFAGSPSNREFSPAHSPWNSGVTPTYAGAAWSPTTGGMSPGAGFSPAGNTDGGASPFNEGGWSPASPGDPLGALSPRTPSYGGMSPGVYSPSSPQFSMTSPHYSPTSPSYSPTSPAAGQSPVSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPSSPSYSPSSPSYSPSSPRYSPTSPTYSPTSPTYSPTSPTYSPTSPTYSPTSPSYESGGGYSPSSPKYSPSSPTYSPTSPSYSPTSPQYSPTSPQYSPSSPTYTPSSPTYNPTSPRGFSSPQYSPTSPTYSPTSPSYTPSSPQYSPTSPTYTPSPSEQPGTSAQYSPTSPTYSPSSPTYSPASPSYSPSSPTYDPNS med21_caeel Mediator of RNA polymerase II transcription subunit 21 mdt-21 C24H11.9 Mediator complex subunit 21 CeSRB7 protein protein caeel Caenorhabditis elegans MADRMTQLQDMINEMAGLMTNAIGVLQATAPPCEFGAISQELEDEPNCAIFAAGIAKAAKNIEILIDSFPIEAGNQEAEVEEKMIKNDEKQREKVNELVGLVGDSNRLVGVVQKKLAEISKTQMSSRPSE med10_caeel Mediator of RNA polymerase II transcription subunit 10 T09A5.6 mdt-10 Mediator complex subunit 10 CeMED10 CeNUT2 protein protein caeel Caenorhabditis elegans MVQQQQQSQQRMMELHERNDREKLARKTEKEREEERRKQEDDKILQLEKKLEEFQENARFIGDLASNFQTKYQDALNGRIYTLVRGLQDLDRMKGTFSDKKVPLDLLPYLDDGKNPCLYSKHCMEKTLEKNKAVNGKIEIYKKFRAHLMKEFSEEMPDLVMYYRSIREDLDLS med7_caeel Mediator of RNA polymerase II transcription subunit 7 let-49 mdt-7 med-7 Y54E5B.3 Mediator complex subunit 7 CeMED7 Lethal protein 49 protein protein caeel Caenorhabditis elegans MLPGFGAQTVSPFPNPPEYASAYTSDRINNGSAPPPPHPLTEFKVYGEEYRLEDDVIAPLKNAGVAELYKNKNNWKTEMKKLNRSAIVAFFDLVEILIRAPDHPMREEKMVDLHTIFINMHHLINEFRPVQARDSVRILQERQIEELSDICKDFKKYLRDGREVVDDQFQMIRGKLPAPPQPSELTRVKLQDGVLHMLQETEASDDVEMKEEEGSEYSKKKARLELLSREDGPPSVVHLLARQFHDISLKK med6_caeel Mediator of RNA polymerase II transcription subunit 6 mdt-6 med-6 Y57E12AL.5 Mediator complex subunit 6 CeMED6 c-MED6 protein protein caeel Caenorhabditis elegans MMPRMGPPAAARQDNPLHVSFRNPQWPPNFINKDNVLDYFCNQANAFYEMNSCNQQIRMQNIVNRTVEECLRTMPGIQYVLWYSQPPLFIICKQRRNNVTNVSPIAYYYVINGSVHQAPDMYSLVQSRLLGALEPLRNAFGEVTNYSRYNTAKGYYWEFKNKPNVKKREEEKKEDEEEKLEDRSTNFQKTRTMMLLNQLFSEMPAEDALEREEKEEVEEEEEETLKTEEPTTSTDEPKFAEPTARTTSKQ med21-med7-2 668042 N/A 668046 unspecified role unspecified role neutral component neutral component N/A 668047 unspecified role unspecified role neutral component neutral component N/A 668048 unspecified role unspecified role neutral component neutral component N/A 668049 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig 2B med6-rpb1-1 668042 N/A 668050 unspecified role unspecified role neutral component neutral component N/A 668046 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig 2B med21-med7-3 668043 N/A 668049 unspecified role unspecified role bait bait N/A 668047 unspecified role unspecified role prey prey N/A 668046 unspecified role unspecified role prey prey N/A 668048 unspecified role unspecified role prey prey association association false false false Fig 2C med6-rpb1-2 668043 N/A 668050 unspecified role unspecified role bait bait N/A 668046 unspecified role unspecified role prey prey physical association physical association false false false Fig 2C med21-med7-1 668044 N/A 668047 unspecified role unspecified role bait bait N/A 668049 unspecified role unspecified role prey prey physical association physical association false false false Fig 2A med6-rpb1-3 668045 N/A 668047 unspecified role unspecified role neutral component neutral component N/A 668046 unspecified role unspecified role neutral component neutral component N/A 668050 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig 2D