IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ floss-1997-1 in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot B Floss, G L Igloi, C CassierChauvat, U Muhlenhoff Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Cross-linking assays on of photosystem I preparation and purified ferredoxin from Synechococcus elongatus were performed using the cross-linking agent N-ethyl-3-(3-diaminopropyl)carbodiimide (EDC) in the presence ofN-hydroxysulfosuccinimide ester (Sulfo-NHS). fig 4 Photosynthesis Research fer_theeb Ferredoxin-1 Ferredoxin I petF1 petF tsl1009 protein protein theeb Thermosynechococcus elongatus (strain BP-1) MATYKVTLVRPDGSETTIDVPEDEYILDVAEEQGLDLPFSCRAGACSTCAGKLLEGEVDQSDQSFLDDDQIEKGFVLTCVAYPRSDCKILTNQEEELY psad_theeb Photosystem I reaction center subunit II psaD tll1724 Photosystem I 16 kDa polypeptide protein protein theeb Thermosynechococcus elongatus (strain BP-1) MTTLTGQPPLYGGSTGGLLSAADTEEKYAITWTSPKEQVFEMPTAGAAVMREGENLVYFARKEQCLALAAQQLRPRKINDYKIYRIFPDGETVLIHPKDGVFPEKVNKGREAVNSVPRSIGQNPNPSQLKFTGKKPYDP petf1-psad-1 Cross-linking of Ferredoxin with PsaD subunit of photosytem I (PSI) in Synechococcus elongatus 366386 N/A 366387 unspecified role unspecified role neutral component neutral component P0A3C9:p.[Glu94Gln;Glu95Gln;Glu96Gln] glu94gln & glu95gln & glu96gln mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 366388 unspecified role unspecified role neutral component neutral component synel Synechococcus elongatus direct interaction direct interaction false false false Numbering in original paper refers to mature peptide without the initiator methionine, therefore differs by 1 amino acid The C-terminal acidic patch was found to be important for ferredoxin-mediated electron transfer since the rate of NADP+ photoreduction mediated by the E93-95/Q93-95 mutant ferredoxin accounts for only 25% of the wild-type. Although the E94-96/Q94-96 ferredosin mutant shows a drastic drop of interaction with PsaD, the binding is not completely disrupted indicating another region of the ferredoxin may bind to PsaD.