IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ weeda-1997-5 The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 weeda-1997-7 The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 weeda-1997-1 The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 weeda-1997-2 The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 weeda-1997-3 The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. pull down pull down western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 weeda-1997-4 The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 weeda-1997-6 The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 weeda-1997-8 The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. human-xpcs2ba Homo sapiens xeroderma pigmentosum patient cell line with mutation in ERCC3 gene. xpcs2ba XPCS2BA patient cell line. This cell line was derived from patients with a single mutation in ERCC3 gene which causes complete inactivation of the NER function of the protein. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 prs8_mouse 26S proteasome regulatory subunit 8 Psmc5 Sug1 Proteasome 26S subunit ATPase 5 Proteasome subunit p45 p45/SUG 26S proteasome AAA-ATPase subunit RPT6 protein protein mouse Mus musculus Mouse MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK ercc3_human General transcription and DNA repair factor IIH helicase subunit XPB ERCC3 XPB XPBC Basic transcription factor 2 89 kDa subunit TFIIH basal transcription factor complex 89 kDa subunit DNA repair protein complementing XP-B cells Xeroderma pigmentosum group B-complementing protein DNA excision repair protein ERCC-3 protein protein human Homo sapiens Human MGKRDRADRDKKKSRKRHYEDEEDDEEDAPGNDPQEAVPSAAGKQVDESGTKVDEYGAKDYRLQMPLKDDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEGEATELITETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK prs8_human 26S proteasome regulatory subunit 8 PSMC5 SUG1 Proteasome 26S subunit ATPase 5 Proteasome subunit p45 p45/SUG Thyroid hormone receptor-interacting protein 1 26S proteasome AAA-ATPase subunit RPT6 protein protein human Homo sapiens Human MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK 29C6410C4A85A7F7 tf2h1_human General transcription factor IIH subunit 1 General transcription factor IIH polypeptide 1 TFIIH basal transcription factor complex p62 subunit Basic transcription factor 2 62 kDa subunit GTF2H1 BTF2 protein protein human Homo sapiens Human MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAENVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPLLDLTALEDKPLDEGYGISSVPSASNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQEAQNEQTSEPSNMDGNSGDADCFQPAVKRAKLQESIEYEDLGKNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAINQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHTWQSRRLMKKT xpb-sug1-4 573201 N/A 573209 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 573210 unspecified role unspecified role bait bait physical association physical association false false false 4A The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. xpb-sug1-5 573201 N/A 573210 unspecified role unspecified role prey prey N/A 573209 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4B The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. ercc3-psmc5-1 573202 N/A 573211 unspecified role unspecified role prey prey N/A 573210 unspecified role unspecified role bait bait physical association physical association false false false 5C ercc3-psmc5-2 573202 N/A 573210 unspecified role unspecified role prey prey N/A 573211 unspecified role unspecified role bait bait physical association physical association false false false 5B xpb-sug1 573203 N/A 573209 unspecified role unspecified role prey prey N/A 573210 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. xpb-sug1-1 573204 N/A 573209 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573210 unspecified role unspecified role bait bait c terminal 21 aa sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 573235 573233 physical association physical association false false false 2A XP11BE mutant with a frame shift altering the last 41 amino acids of the encoded XPB protein was also found to interact weakly with mSUG1. It generated a transcriptional signal 5�10 times lower than the wild-type XPB fusion protein The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. xpb-sug1-2 573205 N/A 573209 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 573210 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false 3A The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. xpb-sug1-3 573206 N/A 573210 unspecified role unspecified role bait bait N/A 573209 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3B The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. ercc3-psmc5 573207 N/A 573210 unspecified role unspecified role neutral component neutral component N/A 573211 unspecified role unspecified role neutral component neutral component physical association physical association false false false 5A sug1-ercc3 573208 N/A 573212 unspecified role unspecified role prey prey N/A 573210 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 573211 unspecified role unspecified role prey prey association association false false false 6 The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database.