IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ dohlman-1996-1 Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Dohlman HG., Song J., Ma D., Courchesne WE., Thorner J. Cell lysates were converted to spheroplasts, proteins overlaid on a 70% sucrose gradient 6 Mol. Cell. Biol. (0270-7306) 1996 Only protein-protein interactions imex curation dohlman-1996-2 Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit). in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Dohlman HG., Song J., Ma D., Courchesne WE., Thorner J. Cell lysates were converted to spheroplasts, proteins overlaid on a 70% sucrose gradient 6 Mol. Cell. Biol. (0270-7306) 1996 Only protein-protein interactions imex curation sst2_yeast Protein SST2 SST2 L9324.9 YLR452C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVDKNRTLHELSSKNFSRTPNGLIFTNDLKTVYSIFLICLDLKEKKHSSDTKSFLLTAFTKHFHFTFTYQEAIKAMGQLELKVDMNTTCINVSYNIKPSLARHLLTLFMSSKLLHTPQDRTRGEPKEKVLFQPTPKGVAVLQKYVRDIGLKTMPDILLSSFNSMKLFTFERSSVTDSIIHSDYLIHILFIKMMGAKPNVWSPTNADDPLPCLSSLLEYTNNDDTFTFEKSKPEQGWQAQIGNIDINDLERVSPLAHRFFTNPDSESHTQYYVSNAGIRLFENKTFGTSKKIVIKYTFTTKAIWQWIMDCTDIMHVKEAVSLAALFLKTGLIVPVLLQPSRTDKKKFQISRSSFFTLSKRGWDLVSWTGCKSNNIRAPNGSTIDLDFTLRGHMTVRDEKKTLDDSEGFSQDMLISSSNLNKLDYVLTDPGMRYLFRRHLEKELCVENLDVFIEIKRFLKKMTILKKLIDSKHCDKKSNTSTSKNNIVKTIDSALMKQANECLEMAYHIYSSYIMIGSPYQLNIHHNLRQNISDIMLHPHSPLSEHFPTNLYDPSPASAESAASSISSTEADTLGEPPEVSLKPSKNLSNENCSFKKQGFKHQLKEYKPAPLTLAETHSPNASVENSHTIVRYGMDNTQNDTKSVESFPATLKVLRKLYPLFEIVSNEMYRLMNNDSFQKFTQSDVYKDASALIEIQEKC gpa1_yeast Guanine nucleotide-binding protein alpha-1 subunit YHR005C GPA1 CDC70 DAC1 SCG1 GP1-alpha protein protein yeast Saccharomyces cerevisiae Baker's yeast MGCTVSTQTIGDESDPFLQNKRANDVIEQSLQLEKQRDKNEIKLLLLGAGESGKSTVLKQLKLLHQGGFSHQERLQYAQVIWADAIQSMKILIIQARKLGIQLDCDDPINNKDLFACKRILLKAKALDYINASVAGGSDFLNDYVLKYSERYETRRRVQSTGRAKAAFDEDGNISNVKSDTDRDAETVTQNEDADRNNSSRINLQDICKDLNQEGDDQMFVRKTSREIQGQNRRNLIHEDIAKAIKQLWNNDKGIKQCFARSNEFQLEGSAAYYFDNIEKFASPNYVCTDEDILKGRIKTTGITETEFNIGSSKFKVLDAGGQRSERKKWIHCFEGITAVLFVLAMSEYDQMLFEDERVNRMHESIMLFDTLLNSKWFKDTPFILFLNKIDLFEEKVKSMPIRKYFPDYQGRVGDAEAGLKYFEKIFLSLNKTNKPIYVKRTCATDTQTMKFVLSAVTDLIIQQNLKKIGII 2E87C546E133D6E5 ste2_yeasx Pheromone alpha factor receptor STE2 protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MSDAAPSLSNLFYDPTYNPGQSTINYTSIYGNGSTITFDELQGLVNSTVTQAIMFGVRCGAAALTLIVMWMTSRSRKTPIFIINQVSLFLIILHSALYFKYLLSNYSSVTYALTGFPQFISRGDVHVYGATNIIQVLLVASIETSLVFQIKVIFTGDNFKRIGLMLTSISFTLGIATVTMYFVSAVKGMIVTYNDVSATQDKYFNASTILLASSINFMSFVLVVKLILAIRSRRFLGLKQFDSFHILLIMSCQSLLVPSIIFILAYSLKPNQGTDVLTTVATLLAVLSLPLSSMWATAANNASKTNTITSDFTTSTDRFYPGTLSSFQTDSINNDAKSSLRSRLYDLYPRRKETTSDKHSERTFVSETADDIEKNQFYQLPTPTSSKNTRIGPFADASYKEGEVEPVDMYTPDTAADEEARKFWTEDNNNL gbb_yeast Guanine nucleotide-binding protein subunit beta YOR50-2 YOR212W STE4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAAHQMDSITYSNNVTQQYIQPQSLQDISAVEDEIQNKIEAARQESKQLHAQINKAKHKIQDASLFQMANKVTSLTKNKINLKPNIVLKGHNNKISDFRWSRDSKRILSASQDGFMLIWDSASGLKQNAIPLDSQWVLSCAISPSSTLVASAGLNNNCTIYRVSKENRVAQNVASIFKGHTCYISDIEFTDNAHILTASGDMTCALWDIPKAKRVREYSDHLGDVLALAIPEEPNSENSSNTFASCGSDGYTYIWDSRSPSAVQSFYVNDSDINALRFFKDGMSIVAGSDNGAINMYDLRSDCSIATFSLFRGYEERTPTPTYMAANMEYNTAQSPQTLKSTSSSYLDNQGVVSLDFSASGRLMYSCYTDIGCVVWDVLKGEIVGKLEGHGGRVTGVRSSPDGLAVCTGSWDSTMKIWSPGYQ 4B0E26040AAA83E5 sst2-gpa1-1 Interaction of SST2 and GPA1 demonstrated by sucrose density gradient centrifugation 358568 N/A 358570 unspecified role unspecified role neutral component neutral component N/A 358571 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false ste2-ste4-2 Interaction of Ste2 and Ste4 demonstrated by sucrose density gradient centrifugation 358568 N/A 358572 unspecified role unspecified role neutral component neutral component N/A 358573 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false sst2-gpa1-2 Interaction of SST2 and GPA1 demonstrated by GST pulldown 358569 N/A 358570 unspecified role unspecified role prey prey N/A 358571 unspecified role unspecified role bait bait c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false