IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ blumer-1988-1 The STE2 gene product is the ligand-binding component of the alpha-factor receptor of Saccharomyces cerevisiae. in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Blumer KJ., Reneke JE., Thorner J. Membranes were incubated with 35S-labeled a-factor, then cross-linking was initiated by the addition of the homobifunctional N-hydroxysuccinimide ester, ethyleneglycol-bis(succinimidy1succinate). Proteins labeled by cross-linking were resolved by SDS-PAGE and detected by fluorography. Identity of prey confirmed by absence of binding in Ste2-minus cells. 4 and 5 J. Biol. Chem. (0021-9258) 1988 Only protein-protein interactions imex curation mfal1_yeast Mating factor alpha-1 YPL187W MF(ALPHA)1 MF-ALPHA-1 MFAL1 Alpha-1 mating pheromone protein protein yeast Saccharomyces cerevisiae Baker's yeast MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKREAEAWHWLQLKPGQPMYKREAEAEAWHWLQLKPGQPMYKREADAEAWHWLQLKPGQPMYKREADAEAWHWLQLKPGQPMY ste2_yeasx Pheromone alpha factor receptor STE2 protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MSDAAPSLSNLFYDPTYNPGQSTINYTSIYGNGSTITFDELQGLVNSTVTQAIMFGVRCGAAALTLIVMWMTSRSRKTPIFIINQVSLFLIILHSALYFKYLLSNYSSVTYALTGFPQFISRGDVHVYGATNIIQVLLVASIETSLVFQIKVIFTGDNFKRIGLMLTSISFTLGIATVTMYFVSAVKGMIVTYNDVSATQDKYFNASTILLASSINFMSFVLVVKLILAIRSRRFLGLKQFDSFHILLIMSCQSLLVPSIIFILAYSLKPNQGTDVLTTVATLLAVLSLPLSSMWATAANNASKTNTITSDFTTSTDRFYPGTLSSFQTDSINNDAKSSLRSRLYDLYPRRKETTSDKHSERTFVSETADDIEKNQFYQLPTPTSSKNTRIGPFADASYKEGEVEPVDMYTPDTAADEEARKFWTEDNNNL ste2-mfal1 Interaction between Ste2 and Mat-alpha1 determined by chemical cross-linking 358507 N/A 358508 unspecified role unspecified role neutral component neutral component N/A 358509 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false