IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ joukov-2006-2 The BRCA1/BARD1 heterodimer modulates ran-dependent mitotic spindle assembly. xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Joukov V., Groen AC., Prokhorova T., Gerson R., White E., Rodriguez A., Walter JC., Livingston DM. CSF arrested Xenopus egg extracts were used in this experiment. david_livingston@dfci.harvard.edu Cell (0092-8674) 2006 Only protein-protein interactions 6 imex curation Xenopus interphase egg extracts were used in an immunodepletion experiment to indicate complete association of BRCA1 and BARD1. After depletion the supernatant was analysed for the lack of the two proteins. XBARD1 and XBRCA1-specific Abs were used to deplete egg extracts of BRCA1 and BARD1. joukov-2006-1 The BRCA1/BARD1 heterodimer modulates ran-dependent mitotic spindle assembly. xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Joukov V., Groen AC., Prokhorova T., Gerson R., White E., Rodriguez A., Walter JC., Livingston DM. Xenopus interphase egg extracts were used in an immunodepletion experiment to indicate complete association of BRCA1 and BARD1. After depletion the supernatant was analysed for the lack of the two proteins. david_livingston@dfci.harvard.edu XBARD1 and XBRCA1-specific Abs were used to deplete egg extracts of BRCA1 and BARD1. Cell (0092-8674) 2006 Only protein-protein interactions 6 imex curation q643y9_xenla RHAMM protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSFPKAPLKRFNEHIGCAPAPGSYDVKLYDGLKGPVSFEKSHRFQAKKDSNNESQIMDKEEELSPVRLRKHSFGSAQNLRQKPEKDADFVKEMKKQKSLEKEIRSLIKERTEQDKKLQALEEEFKKTEVKLVTALREKTSLSASIASMERHIADLDKANELLKTKFSDDSSKKKINSLCAELIEVKNKVDAKDKEIICKQANFEEQITVLQANLLSCKQTLETVQKKNTFLEENYQDANSHSEALEKDLDNARALIEELRGESRNLQDYLSGAQEQMQDMRMEMSTKEREFENKLKDISSSMSEQSKALAIQGEMESKLHETEKNLALALEKIAQLEQELNAVKEEKHKLIEEKAETEAKLCASMEQMGKISTAVSQCEQYKLCLDQTSDLLKQKEQEEMSTKENFSLREKELLAQIKELDEKYLAQIQEKETLASESREKERMLQIELEMVKEKMSKEEDSCHVLREKEKQLSMLLQKEMETCSFLREELQKVQEEMMKERHLLEEELEGTLDELDRLQIAEEHSDRFIKQLEEENKQRAGELDYLEETLKGKSAELERIKEVHRKAMLQLKEEQSNNTHKVQEYDSFRRSASTEIESLKSTNLSLLEKIAQAEKDKEMQEQLLEEQVRDKSVLEQQFIDLRAASSQTKEEYEQKLYELQETSNTTKEGLEQQLLDLHKTSAKAVEDLEEKLYKLQEASSKTKEVLEQQVKDLQEVSNKTRDGLEEQLQKVQEASTKTRESLEQELHELKETSANTNKGLEQQLHEIQKEASRTAEKLEQQLHEQQEVSAKTIKALEQQLKDLLETSTTTKQGLEQQLCKLQEDSTKTKNDLEQQLRDLQEASAKTQDGLEKQLHLLQEESAKTSKLLLQQLCDLQEVSNKTRENLEQQLQEMQETSSKTKAELERQLQELQETSSNTKDLEQQLQDACTKRIEFEQQQHELEELRLQLLALQESTALSKEKYISMICDAELNLEMKDAKLKELEEQQQLREDPLRMELEQLQKQSEQLHELKRKQEEELELLRKQSAKDEQDATEANKWRTLYEELQNKVRPFKQQLDAFEAEKNAMLNEHGAAQDELNKLSEAYAKLLGHQNQKQKIKHVMKLKTENSELKQEVSKLRAQLAKEKQVEKQLQAHLNEIEGVKRFDPSKAFKHDVKENVFPKAPLREGNRNKC p70012_xenla numa1.S NuMA numa1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MTSHSGKMEALLCWVNSLKVDEPIERFSQLQDLSILLKVVGVLSGNSEETAPVLQQDLEQRLNCLCSFLQRYCQTGSSAQNLVQWQKILQGENLEVELSKVIVILFYYSTMSSKNPRKSEDIDHKTQTELASILRFVLDNEDALCLDDKLINFLKRKAPFPSSGNDPSSSSDAMTPNVSHRRKSEVRFLQLHSVASSSTVKSLSADAPSSPMIEVLHTPQFQMRRLKKQLSEVRECRDELEVELAENRKHLAEKEAQISLLQQQIENLRILRENQTELQEPKELEELREKNESLMIRLRDTLKQCQDMKADKKLLERKNDQLAEENGELSYKVRDLSNRLAQLQEALYETTEEQELSLSNWQQKQNQLESELSGAVGEKKYLEEHNLILQGKISMLEDQLKEMGEIDMPETGDCMGDILKLDDLKQELAVLNTQCLSLKEQIHQMEEEKSTAEVEMEAQKSRFESEKGQLQEIVTNLQTSLSEITFQKERLDNEARAQQEHLMCQITTLKLEISKLKSSLVHKDEELKGIHHKVEEERNEKNQLLENFKMLGGNLIGITQQLESKTKEVDYLREQQQKILCERDSTLSTLNEYKCKKDENSGVLNKTVKTLEQDHQTSLSVIEKLKSEKEELASKVQDLDAKMLGLIAKCQNLDSENDSQSKSHAATVESLKAQLSEQESQLKIYRKKVSSNELVSEENSKLKDQLLSVEESLRHLREHLEKEKTKFAASLDADLKRISHLEEEMKKLSESRDEALHNLDEERTAGKKIESQLKHLEEEYQKANESLQAKLAGSCAAIKQREEERDELSKVVDIWKAKYGESQQKIAQNSCHMQEQTEELKKTHSDVYQQLEGERSKVLMIEAKASETKSSQLEKINQLEGELSAANACIKEREAEEKKLVSALHSAEEKLKIAYQGESERLSHLETALSNAKQDLDCLAKELSDEKYKKAEFEAMVKVLKEQNSERIASLESELKNSLAVVKERKCESEKLSGEVEHLKRQLDDSSQKHKEALAQKNIEIKQLINAKEKATSDLAIKSEMGAQLQKAVDTHKSEFSALQNELSRSLDLLALKEGEVERLNKEAALRQEEIQQQQQTITKLTEEETALAALKDKVALQEKEIKQQVQATKGAEKEMAKLKSVISEKSKRIECLEQDIQNQKRDLSCIQEQHQSKLGESQGLQALIADLEKKCKEQKELICEAQNKAAEAKTLASEKASVSERQLEGIQALEIEIGKERQKACDLQKQLELSWAVQEEKETELQALKKELFHKVQELEQSQTSFTDSSGEALLYLSEAQERQQALTEAKEQAEQYQKEIEMKNKEVNSLQAEIKILSSKVTTNEEVSVDFEQRLLKETSKSAKLEEKMQKLHMELEASFKELLEKNCAIDCLTTEAQNLKGEADQQRMAVDSLQQKLSSKAETNHTLQQEIQAWQKNCAEKEQQICSLQQNLKSNQSLLEEFASLKHSYQEIIAERDLMQEKHQEELLSHKKLTERFQAELEKAKEDMTEIVLLKEKLHNQELQLHKFQSENSYSLTQISHLQQVNSQLLGANQSLSQISDQGAKKLESEMSTLKEQHKEEMKTLRLQYEKTLREGNKQVQETSLQLETVTSKYDHVKSKVLKDQKTFQEEKQRLLLQVQELNKQLSQQEKTIRSQQQKLKQREGETHEEADKSHKRVLELESQLEQQTQAVEHYKAQMEKAKVHYDAKKKQNQELSEELQSHIKQQEHLSKENADLKAESEQLHKELQHSLLQSKEVEQNCKNLSNRVRSLEASWSTLTGQLRDPGKFQLATAFHEESGHFCAPRQTRSHADVSIDSLDLSFGEDQLLNSTSERCNEEPATSSVHASSPDSLISGQLPKKVESLESLYFTPIPTRAQSKLESSIGSIGDLSLDSSKKTQSARRRTMQIINITMTKKTKEEPESANTSFYSLRSAPSYQSLHLQNPRRAGRPPAAISAPALASLPSQESLAKTEHFSSDDSLNNLPGYQHPTRRSARLSQTGGRSSFYMSTCQDEPDPQDDWTRIAELQARNKTCPPHLKTSYPLESRPSIFSSTITDEEVKLGDPKETLRRATLLPGQIQDSMTSTRRQTLAVPGAEHLKGHNISTRQQMKRVSEESHYGPDTPEAKKTATCFPRPMTPKDKHDTRRVSTMESKGSSSHQQAQPTRRQATAFSIFNTPKKLGNSLLKRGLNKKTTPKNSPRGRGANGSTSSTSNKPSHLSLRKSPSQRSPRVSTAKSPRASNKLFERKQQRNK q90x96_xenla RING-type E3 ubiquitin transferase BRCA1 brca1.L BRCA1 brca1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MTCSRMDIEGICSVISVMQKNLECPICLELMKEPVATKCDHIFCKFCMLQLLSKKKKGTVPCPLCKTEVTRRSLQESHRFKLLVEGQLKIIKAFEFDSGYKFFPSQEHTKGLDSTIEDVLVKEDQSIVHCKGYRNRKKGVFNRKTYEETGMLSVSKAEEQFAKEVTRLIPCRQKKPKKEAALIFSNCVPDSSDGDLLNKENGLRNDCSPLHYEKEDTQIPEMEEMVESDLAECEFAESAGSNLLGFDGPEGIPEISAETSINAAGNCDFYGRKTEQFPNDHHCSFKQNIADAEQNKRNQHCGNVPFAPMGKSNLDEKETVETDFDNQHNDSNPENNDPLGKVTKLMRRSTERVNEWLLKTNQDFSTLSAEEDPILDALALQNKETSDKRSCSSDDSELMPVLHKHAEKGISGGGFDKPAVGVKDKIFCKVYKRERKAMPPNNITCVAEVHHDSALETGKENMTLEYGTGMSHLMSKRKMVYSLNPENTSKKNDLANINGSINVFPDCISDANLELEDKSEADSNSADACQSDIVHSSNTQIKQQCVESLANAGETRKKQELSCERSQEEKDFTQSGALGPKTRSQKSPYGHSELHIESSQISNEPSNVTKQVEVRRSRRLLMLPKGPGNKSNSNAVKEMNEQENIAQIPEFRVKDTNKNNEISDTVPSQLKKKDTYFPPCTSEDTGELENGIPVRKISDQNASLDNPIDPCKDEYSDTLLHVAGDNVQPDYMETEESELETQHIVKMFKTSKRTSFILESKEAENENRVNSAVEISQVETSNELPNVAECRHTSLLSSAKEQSGALLKQGSPSSEPKKTSPIHMLKKTESKHSKMSRNRRGKVKPSSNSAKNTTGQPDNLNNPTQGVTGSLYNKQVMSDFPMRLNVDEEHTNASAKGSQSSVADKSTAHSNVNTDFISDINSATPDGLLHYMDKAEGNSSPWDTTRDKNAALLESCLPSAYGSTGEKTPIPKVQSSEEGSSQGLFNQKPKCSSSGKADQKNSSKNPGKSQCRILSDFSGSSNNGKCSSQEMLSKAAFPSLSQESQCSVSLFSTQSNMSQQSVDEDHKQDVLQDELVSPNKTKRTAISGTEETQISPLRDQYQNPDIDEASECESEASHTGDSSILSSQDELLNTQQRNYMKDSLKKLQQEMAALEAVLGQHGTQKLEAETTCIPSSEHVTEMQEATEEEEEETYQGENLFVKTPPEHVANGSTLIGQSTDNGLQGRNRPVSPSFLCQTRIEKDTPEIVCPAMGISKKSLLTQKELPSQHKQEIRPDLETVNVTPTNPIDAQESLRQSSKNKRGSRMLHNKRSTSNQGSFCLSVEAAESPQIPKQNKAEFGIARKSTSPTFASPARAKVPSVGFKSPVVSSRRNLSFVASGLNQCEMALVQRFSKTTQSILSSRITDSTTHVIMKTDAELVCERTLKYFQGIASRKWVVSYEWVVQSFREGQILDEYDFEVKGDVINGRNHRGPRRSRLGSDGLLLIDFEICFFGSFTDMTLDDLEWMVSECGATVVKKLQFFKKKHNVTSLVIVQPDASTEVRDYTEIRKKHKALVVTREWLMDSVATYRLQKFDAYLA tpx2a_xenla Targeting protein for Xklp2-A tpx2-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MEDTQDTYSYDAPSIFNFSSFHEDHNADSWFDQVTNAENIPPDQRRLSETSVNTEQNSKVEPVQTTPSKDDVSNSATHVCDVKSQSKRSSRRMSKKHRQKLLVKMKDTHLEKETAPPEYPPCKKLKGSSSKGRHAPVIKSQSTSSHHSMTSPKPKAQLTMPSTPTVLKRRNVLVKAKNSEEQELEKMQELQKEMLENLKKNEHSMKVAITGAGQPVKTFIPVTKPVDFHFKTDDRLKRTANQPEGDGYKAVDFASELRKHPPSPVQVTKGGHTVPKPFNLSKGKRKHEEASDYVSTAEQVIAFYKRTPARYHLRSRQREMEGPSPVKMIKTKLTNPKTPLLQTKGRHRPVTCKSAAELEAEELEMINQYKFKAQELDTRILEGGPVLLKKPLVKEPTKAIGFDLEIEKRIQQREKKEEIEEETFTFHSRPCPSKMLTDVVGVPLKKLLPVTVPQSPAFALKNRVRIPAQEEKQEEMVPVIKATRMPHYGVPFKPKLVEQRQVDVCPFSFCDRDKERQLQKEKRLDELRKDEVPKFKAQPLPQFDNIRLPEKKVKMPTQQEPFDLEIEKRGASKLQRWQQQIQEELKQQKEMVVFKARPNTVVHQEPFVPKKENRSLTESLSGSIVQEGFELATAKRAKERQEFDKCLAETEAQKSLLEEEIRKRREEEEKEEISQLRQELVHKAKPIRKYRAVEVKASDVPLTVPRSPNFSDRFKC q90x95_xenla bard1.L BARD1 bard1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MLLRVRSGNRPSETAVREELPMMDEPGWERTRDALREMERKLCCSKCMFILKDPVCLGGCEHVFCWTCVNENLGNECPLCNTPAWVRDVQVNRQLDNMIQLCAKLRNLLSMGKTDENKMDLCQDTLLRLNPAQEKQKKKQIKMWFSPRSRKVRCVVEKKEKSQQISSKTLPQMASYEFVSDSPDGEPAQKKPDPKPRKRMKKKLKDINAEWGVDTENEIDEVSNPRKEHKSGKSVSFCSSAVIQHSPEVLVVESSLKNDTPEEISPNWNKCGLDEPMEMQSTSMKEVNCPSPSEQSTTLKTQDKEKKITTVKRKKHSSPIASSKRPRRRSSDTSKPLNDSDIKANSPEKACKTVSPHKPSENSAKDVRIESTQAAKRDTKMCSTPLPCAKQNNSAKLGQKSTAMPISPNNLTNVKRNYKGETMLHLASIKGDIQGVEDLLNSGANPNVKDNAGWTPLHEACNLGHTMVVQLLLQHHALMNTTGYQNDTPLHDAVKNGHIAIVKLLLSHGASRDAVNIFGLQPVDYAETEEMKSVLLETQTERDRLLLHPCLAAPQLSSHQRKEEAVVLIASGLLATQRADLTKLAKMLKASICAEYDSTVTHVIVSDEPVLRTMKCMMGILAGCWTLQFSWVKACLQSCNREPEESYEIPHGPHRARLNGQQMLPQLLDGCHFYFLGCFTEHRKEDLIELVKAAGGQILIRQPKPDSDVTQTINTVAYHAEADSDQRFCTQYIVFDRASKYRPERVRQGKVWFAPSSWIIECITSFQLLPVH brca1-numa 568360 N/A 568362 unspecified role unspecified role prey prey N/A 568363 unspecified role unspecified role prey prey N/A 568364 unspecified role unspecified role bait bait association association false false false 6A brca1-rhamm 568360 N/A 568364 unspecified role unspecified role prey prey N/A 568365 unspecified role unspecified role prey prey N/A 568362 unspecified role unspecified role bait bait association association false false false 6B brca1-tpx2 568360 N/A 568365 unspecified role unspecified role bait bait N/A 568364 unspecified role unspecified role prey prey physical association physical association false false false 6B brca1-numa-1 568360 N/A 568364 unspecified role unspecified role prey prey N/A 568366 unspecified role unspecified role prey prey N/A 568365 unspecified role unspecified role prey prey N/A 568363 unspecified role unspecified role bait bait association association false false false 6C brca1-bard1 568361 N/A 568366 unspecified role unspecified role prey prey N/A 568364 unspecified role unspecified role bait bait physical association physical association false false false 1B brca1-bard1-1 568361 N/A 568364 unspecified role unspecified role prey prey N/A 568366 unspecified role unspecified role bait bait physical association physical association false false false 1B