MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint tafforeau-2006-1 Repression of ergosterol level during oxidative stress by fission yeast F-box protein Pof14 independently of SCF. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tafforeau L., Le Blastier S., Bamps S., Dewez M., Vandenhaute J., Hermand D. damien.hermand@fundp.ac.be A fission yeast cDNA library was used EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation tafforeau-2006-3 Repression of ergosterol level during oxidative stress by fission yeast F-box protein Pof14 independently of SCF. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Tafforeau L., Le Blastier S., Bamps S., Dewez M., Vandenhaute J., Hermand D. damien.hermand@fundp.ac.be A fission yeast cDNA library was used EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation tafforeau-2006-2 Repression of ergosterol level during oxidative stress by fission yeast F-box protein Pof14 independently of SCF. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tafforeau L., Le Blastier S., Bamps S., Dewez M., Vandenhaute J., Hermand D. damien.hermand@fundp.ac.be A fission yeast cDNA library was used EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation tafforeau-2006-4 Repression of ergosterol level during oxidative stress by fission yeast F-box protein Pof14 independently of SCF. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Tafforeau L., Le Blastier S., Bamps S., Dewez M., Vandenhaute J., Hermand D. damien.hermand@fundp.ac.be A fission yeast cDNA library was used EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation skp1_schpo Suppressor of kinetochore protein 1 skp1 psh1 sph1 SPBC409.05 P19/Skp1 homolog protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSKIKLISSDNEEFVVDQLIAERSMLIKNMLEDVGEINVPIPLPNVSSNVLRKVLEWCEHHKNDLYSGTEEESDIRLKKSTDIDEWDRKFMAVDQEMLFEIVLASNYLDIKPLLDTGCKTVANMIRGKSPEDIRKTFNIPNDFTPEEEEQIRKENEWAEDR pof10_schpo F-box/WD repeat-containing protein pof10 pof10 sbp2 SPBC1703.06 Skp1-binding protein 2 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKSEPTSLDFTSSNLRRMNRDHSSNNTNRTVLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRTAFFHHFAGDQSQIFSPLGNGTWRQEYLLRSTITRAYEKGKGQTVQYDCRVGQLTNLYYDFSSGRLYSGNWLTGTISVSDPTTGKVERSLLHASTDGSFTHGLSTMTLGKQIFGFGFMDGRVGVILMSRQAETPRKFRYCLDSHADSVTCIDALTGDLPPTGEIGMVTGSDDGSVHCWDVKTGVSLQSFQFRSSQILSLCFRPKYKMLLVDTFNYELNSYQLYLIPGYARSRKNEQPILLSSRKCVLTDEEEPPCLMTADCCAGVAFLSRGAPKNCICRVSFKEFLEKNDNVGVQTSSIPLNGKPTSISLDTNDRVLSKSTPGRGARLLAVGDENGLVYVVNTRTEDPNKAILRTITAYSNFPITDIYLNEVAMVVGSASGYCGVYDTVTGNFLKKIASARNAARREPINCILLDSNPLSLKGVITMSKHVKSWSYTIPKPFVNKRSKVLPLRPSVTHDNLSKSSDYSKNEVEREIMLGLDQIAQERREKMEARQKFEQHFGEGLVGLSEEEIIAYVTMLSQEEEAKRMVQLSMDVDKIEEDFKENDEQATSSLNALSSNHEPPQEQANVAELNEQEQIELAMRLSLMEM pof14_schpo F-box protein pof14 pof14 SPAC13D6.01 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLQFSFQNCKTHTCYMNTSVNEHLDQDESFLRILIDTRKKIRSSYFKPQTFDEFVHCLARVRAMKRLVSICSNFDEEDNWNGVWNTLVVDGDSHASAMIDYEGLLDKCSLSRNLLELINDYAEYTTQVLPFRKIPSSDNLDSSLNLTVASPKSNLYYRYSSESPKYAKILDCPDEILQLIFSYCYDASYIEKLPFAFSYRKQRHTLIHDLPNTCLRFKKILSPRNVSFWKRLLKVHKKNPNSAVTCSESINTSAVAKFTDIPTIIPIFLDPSYQSYAAKTIHSDTSSLASSIIQTGDGTQSCDESTYIELACNLVGRCVLCNRIPKLTKFKDCITSFYRPSVATNICDQCLEAIIDFYEPVSRYKFDVMKDRLGVGYISRPGQKFPSWLSEHVQLARDEEKAFKSIYHSQGEFARSLFRLFRAQLAELCEQ g6pd_schpo Glucose-6-phosphate 1-dehydrogenase zwf1 SPAC3A12.18 SPAC9.01 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSANLSIKENGAMVVFGASGDLSKKKTFPALFSLFSEGRLPKDIRIVGYARSKIEHEDFLDRITQNIKIDEEDSQAKEKLEEFKKRCSYYRGSYDKPEDFEGLNSHLCEREGDRSTHNRIFYLALPPDVFVSVATNLKKKCVPEKGIARLVIEKPFGVDLKSAQELQSQLAPLFDEKEIYRIDHYLGKEMVQNLVHLRFCNPVISHLWDKNSISSVQITFKEPIGTEGRGGYFDSSTIVRDIVQNHLVQILTLLTMETPTTFSADDLRDEKVKVLRRTRLGDLKDIVLGQYVKSKDGKKPGYLDDETVPKGSRCPTYSAIPCFIDTERWRGVPFLLKAGKAMDIGKVEIRVQFKAAANGLFKDAYHNELVIRVQPDEAIYFKMNIKQPGLSEAPLLTDLDLTYSRRFKNMKLHEAYEALFLDAFAGDQSRFARIDELECAWSLVDPLLKYMEEEKPVPEPYEYGSDGPECLYSFLKKFGYIYDSPDYYDYPVMSVPSDH ap1_schpo AP-1-like transcription factor pap1 caf3 SPAC1783.07c Caffeine resistance protein 3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSGQTETLSSTSNIPIAKAEPEQSADFSASHKKRGPVSDRSSRRTSSEEVDLMPNVDDEVDGDVKPKKIGRKNSDQEPSSKRKAQNRAAQRAFRKRKEDHLKALETQVVTLKELHSSTTLENDQLRQKVRQLEEELRILKDGSFTFEMSLPHRNPSLSSLPTTGFSSNFAHMKDGISPQSNLHLSPNSIEKPNMHQNVLHNDRSADNLNHRYQVPPTLVDSNSAQGTLSPETPSSSDSPSNLYLNYPKRKSITHLHHDCSALSNGENGEDVADGKQFCQKLSTACGSIACSMLTKTTPHRASVDILSNLHESTVSPPMADESVQRSSEVSKSIPNVELSLNVNQQFVSPFGGTDSFPLPTDTGLDSLFEPDSAIENSHLKNVVMEPELFQAWREPAESLDKEFFNDEGEIDDVFHNYFHNSNENGDLITNSLHGLDFLENANESFPEQMYPFIKHNKDYISNHPDEVPPDGLPQKGKHDTSSQMPSENEIVPAKERAYLSCPKVWSKIINHPRFESFDIDDLCSKLKNKAKCSSSGVLLDERDVEAALNQFN ydri_schpo Uncharacterized protein C16E8.18 SPAC16E8.18 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEVTSFILNATFKEFACFGNNYLIILPGIMLERNVFRHLNYSTNSICSHYQFFGGHYESFELLVVIVYYFSHVGSFSLAEIYRITWDKRIVLYGTTTTLVYCSEGSD pof6_schpo F-box protein pof6 pof6 SPCC18.04 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKPSEAREHAIRKIKEYLERRHKQQFKALFGCLTINIYLKIFTLISTPDLCNCRLVCRKFQQLCDYNSIYVKKLLLMNAWDVTLSRKLYYESQDGMSQANMSSNTSKGFHLQSSDKKYADSDRPKLSSSSLLSLPDQKIPFPVDYSTVQGKQVVLTVLDCVSQRVEFARLDYIKVWRALAPIYRNLAYASNTIDPIAFSAFRTPEEQSKVLKYLIRFGNSYPYGTYRQAMQNAILDMASLFEQACLDEFELGLHSRNLDLLRKFSHVLHDFSGPNAYVSMYLAKQTDFVRSFFHFDPYSLFISNNLEEIHINWNILESVVNDTIKLLESESKFSEATLPEPELVQVPYAKDILGNSLKDYVISICEHIGEEETELFLVFISGFYGLCKKFFSIPNGPALVDTIFQPQIDIFISQELHYFKTVGWSLVDQWDQKLEEKEDATECFFYKNVSQNTAKNNFLETFKNVMLLPVSLFTIPSENNSASNLAEKAIEQKEEEDPELSKLDAARFVPANIYVSKDRLKHLPTTELAAQAAVLDSKLEGISTMFSLELALKIVHLCKVSLARAKVFMGTSVPQDDDIKGLSKDLFVQLLRELGQGHLKHGFDRAIEHLSSFDPRRDFSSNTVEPVVKFLELINVGDMIQQMMDSFFNEEMSPICVKDDMFDPAIAEKKKFEQLLDERAAFGLHKGINVLIEHADFLLETKTPMNLFSDQTIGSITNTIEPTAAAKNVVQFLGFHMRILVGRADHEILDVFYKEVGMRLFDSLTRYIKSHKFSVDGGLKLLSDCNLYYEFIHSLHQSSLLPYFKTLKEIAHLFIIDGKNAEEIGKLATDTSRFSSAFHTEEVYEILHSRIDWLNIKYEVDKVIHGLACCIM pof7_schpo F-box protein pof7 pof7 SPCC1827.08c SPCC70.11c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSTVEDQLSLELETVNKDILLKKALLHYKDAIKSEREGNLGESLNKYRLAHKVHEDVESIYRRLERLQLCKRNEEEEMLNSDASEAMLTVSSVPSPTLTENESVNESVVPNILKLPDEVLLVILENCIRDLHDLRYLSSIALTCKHFAKALRADSLYRSFCYCSCEQKEWQQSIKSIEEELVEKYQQSWKTLFLKKPRSRFDGCYISVCRYFRPGTSDTSWNQPIHLITYYRYLRLYPNSTCIVYQSSNEPNDVVRNFSVQNTSLFSPMSSSPMFSNGNVALTGSWSMTPSGEMLIVYPASQTYTYVQKLQVRGIRLKWISFYSIHNYTSFTNEMPLTHNRDYVFSRVYSYTADSESLKRG mcm3_schpo DNA replication licensing factor mcm3 mcm3 SPCC1682.02c Minichromosome maintenance protein 3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTELLADEVFKDRVRIFQEYLEHDTDDANVTLYQEAILRMLNMGQRRLIVNIDELRDYNRELADGVLLKPLEYVEPFDEALRNVVSTLIDPVVHKDLKDKLFYVGFRGSFGDHHVNPRTLRAMHLNKMISLEGIVTRCSFVRPKVIKSVHYCEATKRHHFKQYADATMNGGLSFQSTVYPTQDENGNPLSIEFGFSTFRDHQSISLQEMPERAPPGQLPRSIDILLDDDLVDTVKPGDRVNIVGQYRSMGSKTSGNTSATFRTVLLANNVVLLGNKPGLGNVGGGALDITDADIRNINKLARKKNVFELLSTSLAPSIYGYEYVKQAILLLLLGGTEKNLTNGTHIRGDINILMVGDPSTAKSQLLRFVLNTAPLAIATTGRGSSGVGLTAAVTTDKETGERRLEAGAMVLADRGVVCIDEFDKMSDIDRVAIHEVMEQQTVTIAKAGIHTSLNARCSVIAAANPIYGQYDIRKDPHQNIALPDSMLSRFDLLFIVTDDIDDKKDRALSEHVLRMHRYLPPGVEPGTPVRDSLNSVLNVGATNAAGVSTENVEQEVETPVWETFSSLLHANARTKKKELLNINFVRKYIQYAKSRIHPILNQATAEYITNIYCGLRNDDLQGNQRRTSPLTARTLETLIRLSTAHAKARLSSVVEVKDAKAAEKILRYALFREVVKPKRKKHKKQRLEAGEEFDSEDDNSDDMDIEESEEEMDTNMVIDSGSRRVTRSQNATSQSQESGSEIGSSIAGTAGSYNVGTSNTQLSWPSTHSTLPATSRELASSDRNINTGTSVASEVSASVSEQSTVSLPREKMSVFMARLASLTKSELFSEECASLEDVLESINNIEDDVGFSREEAIVALKEMDAQNKIMFSDNVVYRI pop2_schpo WD repeat-containing protein pop2 Proteolysis factor sud1 pop2 sud1 SPAC4D7.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLSRCPTDNSSSRINSSVPLINSSSPATPPESFDPQVFPSSLIHGDNLLPQDDQIASDPRSESNSCNGNTSSSLPCTDSYQYPLKHSCTPSFLRKFNESIENVSYKCLDHSPPDSVPGDFSISLVPQRNFLYSHSSLPPKIISIDRNNRIKLDNSISSNSDNFPPSPKVDTSNTVSPGSKPISEDLEDLNLQSIVQTFEDLPEGIQSYAFFQLLRSCNRQSMRLLLNECEPLLKKDILSNLPFSIVQSILLNLDIHSFLSCRLVSPTWNRILDVHTSYWKHMFSLFGFQINENDWKYANPNLNRPPFLHNDQISDDYFPEIFKRHFLNRKRWLFPSIPPSHLSFPIHVPNFMITSLLLHKDRIITTSGSGTIQIHNAITGVLEARLEGHKEGVWAVKIHENTLVSGSIDKTVRVWNIEKAKCTHIFRGHISIIRCLEILVPSRLIRHGVEIVEPDQPYIVSGSRDHTLRVWKLPKNTDPPYLPDNTNSIDRWEKNPYFVHTLIGHTDSVRTISGYGDILVSGSYDSSIRIWRVSTGECLYHLRGHSLRIYSVLYEPERNICISGSMDKSIRVWDLSTGTCKYVLEGHDAFVTLLNVFQNRLISGSADSTIRIWDLNTGKPLMVLPSNSGYISSFVSDEHKIISGNDGSVKLWDVRTGKLLRFLLTDLTKIWHVDFDAMRCVAAVQRDDQAYLEVINFSGSRP rm15_schpo 54S ribosomal protein L15, mitochondrial mrp15 mrpl15 SPBC1539.01c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLTRHCNRLGLQIENKFVFRSSSWNCVRRIGKIACNENKYRYEMTSTEEDIDSFFSRVFGEKNYISDKSLREQIVTHKSYKHGLKRYNEPFVSVGNAVIDVCLLNHYLESGNFSTIPSYKDLVNLRSPARLAEVAKKWNPETATQCILPLKGPNSPSSSSFEKIYSSIVSGLVAVVYFQKGGVEAMNFCKKSIISDLTKDFVRPNTVHNQ ral2_schpo Protein ral2 ral2 SPBC21.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSEVKRNISLRNSTNIFTSTFSLSSNNVPKPLIGESVIKYGDEAFVYGGRDALNAQLVNDMYVVDLNTCSWKQVEYQGNQKPIPRYFHSGDLWNNKLIFFGGMGFNDDTKCLYVLNDIDIYDIETKQWSHIPGMITENQTNDDAKEVNGSDVDEKSKHLYPSARYGHLHCVLDHYLIIFCGQDLSNSYIEEINIFDLDSGKWVFKSLFNHHCGIYRSNCVVINKDSEFLQMCRPINTTQDSNEHSIGSLFFYLNYNFVNVKRQVIYLELFELDTAESEKKSAALAKDNNQSFRFLELDVTEKFLSSAMPPGLRFPAVNILGDNLILSGIYLTSSRQAFVLWVYSLDKELWLQLDMLGVLNHGSWFKCLVLDCTNRFVVFGNKTRKLTQDYNLRQSNYDHIVFIELEGYGVYRKPQMGRVTERSEQLGKLLLNGISDMEILTIERMHIPCLSRMLYKRWPAFQKIMDRAVEKNQEAFQAEVSQLGPQLTDLPFSSIHSTGSRALYMPYSFETCSAFLHYIYCGTLNGSYCTAKNLCNLLILCKGFEGLETFFAYIVHLLHGVLNRNNVKLIYETAALTGAKGLQLRALRRIARIEQGGTAISPTSPLPNLDD alp7_schpo Microtubule protein alp7 Altered polarity protein 7 Transforming acidic coiled-coil protein mia1 alp7 mia1 SPAC890.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSDIVSSSTDYSRRSPSSSSIGTNETDHTGFHEKRQGASSESLIPPAQRSSEESMPAPKLFPKLTSKPNPQLNLKDTLNKRVSDRLQALELNKSFDFSGTPRPMHPISHPLSQHKTPEFKHRKRNVESILTPKNPSLFSSSNAASQRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSNGSFPRQTNTAPLNPPVHLKDNIRNSATPSTSQADIPTQYPINSTQKQQAKYEAEIEGYKAKLAGTYHEISVLQNTIVNVSGQLIAVNDQLQQLRSGKASTSPSTKDTNMRLVEGHNEETLALQRGKYTQEEVDKLIQERMEKVAEDLHAQYSAKHTQKINAFKANYARKYEATIQELQNQIGTAPNAPKISNSNWEEERRALKADNQTLQKQLEKAIQERQDMSDFLNNFKADMAKSDKLLMQQQSQQTGDLETLRLQLQALQEELRVEREERQQLIQMSEDLVIAMDQLNLEQKS 22B4216C196E310C ybx1_schpo Uncharacterized protein C17D1.01 SPBC17D1.01 SPBC17D11.09 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKAVYLDYLLRIRYTYVFNLKVSYQSERRTYGATEHFVGCLVPKAWNCLADDYTDIKTLLDLLFGVTAVIIFACTFKCFTMTEDGLTKLNLEAFLGVSGNTQELLESLGISAIPDLSNLGASNNNNSGLTFDPNDPSAAAFYIAQQVAAIEHPPPRKMTSSEKTRSENRERKKRWREQNEERNKDNDLRCRVNKKAKKLFGSEPSIEKTNWIEAEFTRRHTKRKEKERACLSQNQSSNASRANSQSLLPDLDLLATNSSANAVLQGSAINNALNALAKDPNLIHSLLTQIDFDPSKSKDGLNLGSLTDVVPPQPPQPEHELAALQEHNEAHDAAMRQYELERQQNAMLPGLASIDGLQALPHLDFSDSAVTNQSHSQSQNSLHPLISQSETHSIFSALSETPTPVSGNGNVADAPPDFSLSQVMPLDLIAPSMQPSAVSSPMSESHVQISSEMPRTGMSALPMRPRSQNVDKHRKFPYYNKRNAVTTPSSPYDGAQSGSPQFPPFELPPHNYSQAQSPNLATPSPSFSSLPDVSLPPIVKPNLMSEPTPTNSDEKSHALGFPPPPGQKIEFQRSSSKNGTAKSD pgk_schpo Phosphoglycerate kinase pgk1 SPBC14F5.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLSTKLAITDVDLKGKNVLIRVDFNVPLDGDRITNNARIVGALPTIKYALEQQPKAVILMSHLGRPNGARVAKYSLKPVAAELSKLLGKPVKFLDDCVGPEVEKACKEAKGGEVILLENLRFHIEEEGSAKVDGKKVKADASAVEAFRKSLTSLGDIFVNDAFGTAHRAHSSMVGVDLPRVSGFLMKKELDYFSKALENPARPFLAILGGAKVADKIQLIDNLLDKVNRLIICGGMAFTFLKVLNGMKIGDSLFDEAGSKNVESMMAKAKKNNVEVFLPVDFVTADKFDKDAKVGSATAEEGIPDGWMGLDCGPKSSAKFAEVITTSKTIVWNGPAGVFEFDNFAKGTKSMLDACVKTCEAGNVVIVGGGDTATVAKKYGKEDALSHVSTGGGASLELLEGKALPGVVALSSK lcf2_schpo Long-chain-fatty-acid--CoA ligase 2 lcf2 Fatty acid activator 2 Long-chain acyl-CoA synthetase 2 SPBP4H10.11c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDIKDYYCVETKDSKQPGESYIYRSIHSAGKLLDTPEDGVSTVYDLLCTAAKNHGEKQAMGSRKLIKENREEREIIRKVDNEEVVEKKLWTTYELGPYEYISFNKVYEIALALGSGLVASGITSETTMLFFAATSAKWFTTAQGCSSQAIPIVTAYETLGEDGIYTSLDECKSRAIFTDPNLIPKLLGPLKQSTWVKLIVCSSTPSEDLVELVKSTAPDVEIITYDNLLSLGKEKPQPPHPPKADDICCYMYTSGSTGKPKGVVLLHRNIIAALGGINRILSQHINVKDYVLAYLPLAHIFEFIFEMCCLYWGGVLGYASPRTLTDASVVNCKGDLTEFRPTVLIGVPAVYELIKKGILAKVSSMPAHRQKVFSGSLSLKQYLIERNLPGSAALDAIVFNKVKAATGGRLRYCISGGAALAASTQAFLSSCICPVLPGYGLTETCAGSFVLSPEQWHLYANTVGFPIPSIEFKLVDIPDLGYYTDSSPPRGEVWIRGPAVCNGYLNRPEDNKAAFTEDGWFKTGDVGEIAKGNTLRLIDRKKNIVKSLNGEYIALEKIEAQFFTSPLVSNVCAYADVNHAKPVVIVNPDENGLRTYLTKNSGSSFNGNPNDTLTNLCKDSGVQHLILKELINIGKQQRLASIEIPEGVVLSDFEWTAENNFLTASRKVKRQVIVAHYSDEIQKAYSKKH ecm33_schpo Cell wall protein ecm33 ecm33 SPAC1705.03c SPAC1F2.01 SPAC23H4.19 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLFKSFALTLLFAAARVQAASNCSSGPYNISAQGTLDELNSCTVLNGDLYISDAGNSGITTLTVNGIESVQGDVVVSDGQYLTSLSFPSLKNVSGAFNVNNMIRMNNLATPELTSVGSLNLAVLPNLQELQFNAGLSDSDSVVIDDTQLQAIDGISLDSVTTFQVTNNRYIQEITMEGLESAQNIQISANSKGVSVNFSKLSNVTTATFDGISNVFIGNLKSAAGNLYFSNTTLDNISVPYLTEIGQSFAVLYSPELTSLNFPNLTTVGGGFVINDTGLTSIDGFPVISEIGGGLVLLGNFSSIDMPDLSDVKGALTVETKATNFTCPWSNDDSVIKGDDFTCQGSVATISATSSYDLSSTVSATSGSATSATGSATTTSYSSDSSASSSSSSSHESSAASNGFTAGALVLGSLLVAALAM ei2ba_schpo Translation initiation factor eIF-2B subunit alpha tif221 SPCC11E10.07c eIF-2B GDP-GTP exchange factor subunit alpha protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVESDSSGIVRHSQGEFDIVQVYKKFLQDDPEITMPVAAIEALVQLLSRSQAKTISEFMDILQNGSNTLKEGVQNNISLSAGCDIFQRFVTRSLHDVGDFEQCKRHLVENGKLFIQRARACRQRIAHLGYPLIRDGSVILTHGFSRGVAAVLLAAAKRHVRFKVFVTESRPSGSGCLMTRTLKNACIPTCMVLDSAVSFTMNRVDLVLVGAEGVVENGGLINQIGTFQLAVFAKHAHKPFYAVAESHKFVRMFPLSQYDIPFSRPILEFDDPSPETVHPEPEPIPTPSDAIHNELIMNEEQIRNNPTLDVTPPEFVSGLITDLGIIDSKSGVSEELIKLYL 6FAACEBEBD50995A yfy2_schpo Uncharacterized N-acetyltransferase C9.02c SPAC9.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVNIRNIERSEVEEVSKLEAICFPEAERASFARIKDRVKRAPEIQLGLFAPTQHTSTLIGHVLTSRTGSETVTHESMSKHDSKGENIAIHSLSIHPAHRKNGYAKKLIEELQFRCQHTITPKPSRMILISHKPLVPFYESLGFKNLGPSECKFGDETWYDMTHKI tfb3_schpo RNA polymerase II transcription factor B subunit 3 RNA polymerase II transcription factor B 38 kDa subunit RNA polymerase II transcription factor B p38 subunit CDK-activating kinase assembly factor MAT1 homolog RING finger protein pmh1 pmh1 mcr1 tfb3 SPBC776.18c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDDEGARKVDEKCPLCQADRYLNPNMKLLINPECYHKMCESCVDRIFTTGPAQCPTPGCNKILRKAKFREQTFEDAQIEREVDVRKRISRIFNKGQQEFDSLQAYNDYLEEVEILTFNLIYKIDVEETEEKVKQYEKQNRDSIAANSARAAAEARILAQNEILLKRQKQEAREAAIREHQKEKERREQVEQQIIFDLATSGKDPNKIIQLSDSLKKQQENIASSVSNISRSSSILLSDVQQVAEDTTPFSPLAGEKDGSKYFSYSKNTYQDLYLEKVSHEPGRKCGFRIEDCHLRCLYEAFSGIDYDLESLKKLEVAS fdft_schpo Squalene synthase erg9 SPBC646.05c Farnesyl-diphosphate farnesyltransferase FPP:FPP farnesyltransferase protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLANRIEEIRCLCQYKLWNDLPSYGEDENVPQNIRRCYQLLDMTSRSFAVVIKELPNGIREAVMIFYLVLRGLDTVEDDMTLPLDKKLPILRDFYKTIEVEGWTFNESGPNEKDRQLLVEFDVVIKEYLNLSEGYRNVISNITKEMGDGMAYYASLAEKNDGFSVETIEDFNKYCHYVAGLVGIGLSRLFAQSKLEDPDLAHSQAISNSLGLFLQKVNIIRDYREDFDDNRHFWPREIWSKYTSSFGDLCLPDNSEKALECLSDMTANALTHATDALVYLSQLKTQEIFNFCAIPQVMAIATLAAVFRNPDVFQTNVKIRKGQAVQIILHSVNLKNVCDLFLRYTRDIHYKNTPKDPNFLKISIECGKIEQVSESLFPRRFREMYEKAYVSKLSEQKKGNGTQKAILNDEQKELYRKDLQKLGISILFVFFIILVCLAVIFYVFNIRIHWSDFKELNLF pmgy_schpo Phosphoglycerate mutase gpm1 SPAC26F1.06 Phosphoglyceromutase MPGM BPG-dependent PGAM protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTTEAAPNLLVLTRHGESEWNKLNLFTGWKDPALSETGIKEAKLGGERLKSRGYKFDIAFTSALQRAQKTCQIILEEVGEPNLETIKSEKLNERYYGDLQGLNKDDARKKWGAEQVQIWRRSYDIAPPNGESLKDTAERVLPYYKSTIVPHILKGEKVLIAAHGNSLRALIMDLEGLTGDQIVKRELATGVPIVYHLDKDGKYVSKELIDN mug64_schpo Meiotically up-regulated gene 64 protein mug64 SPAC56F8.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast METFKTHITGQFNATVSSITPFAKKTTHYLRTKIGSRVEELSLPEDYVELEQQVDSLKEAYNLVLPIVETVEVDGYDYPTNFRDSITDFGKTVSGKVRNLGNLTPLEQTPLASVGKNLEEKEAAAKPSRTLYNAISRAASEATTKMGAGNPLSSAFGQISVLEEKVGNLQGERDSAISKNFCEQVRAVLYPRFAEAHRVKADVQDKRLQLEMAKLDVESAKPENLEHCKSAVRSAEDELNGAIEHAKILYEQILNKDYNTDLLRSIIKNQLKFHQDAAAALSDITI eif3a_schpo Eukaryotic translation initiation factor 3 subunit A tif32 SPBC17D11.05 eif3a Translation initiation factor eIF3, p110 subunit Eukaryotic translation initiation factor 3 110 kDa subunit protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAPPQGKPENVLRLADELIALDQHSSALQSLHETIVLKRSRNAQGFSLEPIMMRFIELCVHLRKGKIAKEGLYTYKNAVQNTSVTAIENVVKHFIELANKRVQEAQEKADKISVEYVDDLEATETPESIMMSLVSGDLSKSRTDRALVTPWLKFLWDAYRTVLDILRNNARLEVMYQLIANSAFQFCLKYQRKTEFRRLCELLRSHLGNASKFSNAPHSINLNDAETMQRHLDMRFSQLNVAVELELWQEAFRSIEDIHSLLTFSKRAPAAVMLGNYYRKLIKIFLVCDNYLLHAAAWNRYFTFTNVQKPATANFVILSALSIPIIDANKLSGPSIEAEDAKSKNARLALLLNLSKTPTRETLIKDAISRGVLSFCDQAIRDLYQILEVEFHPLSICKKLQPIIKRLAESNDTAQYIRPLQQVILTRLFQQLSQVYDSISLKYVMDLATFEEPYDFNPGQIEKFIMNGNKKGAFSIRLNHIENSISFSSDLFSNPIKSSDSVSLQSTPSELITSQLTRIAKSLSSVLMRFDTDFCLLRKQQAEAAYERAQAGVEQERKAVIAQRSLLELRRGQADTLATQREAELAAQRALKQKQESEAESLRVQEEINKRNAERIRREKEAIRINEAKKLAEELKAKGGLEVNAEDLEHLDADKLRAMQIEQVEKQNKSMNERLRVIGKRIDHLERAYRREAIPLWEEDAKQQAEHDREIFYEREKQRKEVQERKHEQAIKDKKAFAQFASYIHAYKQNIDDERDKAYQEAYAKAKNVIDAERERQRKEIFEQKLAEAIREAEEEAARAAEEEANRELHEQEEAQKRAIEERTRAAREAKEREQREMAEKLERQRRIQQERDEEISRKLAEKAAARRANIGASSPSPGAWRRGGASAGGVSRDSPRYSRGGYSRGSVPPRETLAPSKGAYVPPSRRNQQQQ cul1_schpo Cullin-1 cul1 pcu1 SPAC17G6.12 Cell division control 53 homolog protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTTLNTNDKDLPIVKKYDSLNGTWDFLKTGVSQIFERLDEGMTITKYMELYTAIHNYCADASKTITVDNFNDQTANVLGEALYNNLVLYLEEYLARLRKECISQTNHEEQLAAYAKYWTRFTTSARFINHLFGYLNRYWVKLKNRFTETLVYDIYTLCLVSWHHHVFSHIRDSLLQNLLYMFTKKRLYEPTDMKYVEVCVDSITSLSFDKTDMTKPNLSSYKTFFETNFIENTKNFYAKESSEYLASHSITDYLKKAEIRLAEEEELVRLYLHESTLKPLLEATEDVLIAQHEEVLHNDFARMLDQNCSEDIIRMYRLMSRTPNGLQPLRQTFEEFVKRSGFAAVAKIVPQVGGEADVDPKEYMEMLLSTYKASKELVNTAFHGDTDFTKSLDTAFRELVNRNVVCQRSSSRSPELLAKYADSILRKSNKNVDIDDVEDCLSSIIIIFRYVEDKDVFQNFYTKLLAKRLVNGTSNSQDAESSMLSKLKEVCGFEYTSKLQRMFQDISLSQEITEAFWQLPQSRAGNIDFSALVLGTSFWPLSPNNVNFHLPEELVPLYEGFQNYYYSCHNGRKLSWLFHLSKGEIKARINPQTNVTYVFQVSTYQMGVLLLYNHRDSYTYEELAKITGLSTDFLTGILNIFLKAKVLLLGDNDKLGDPNSTYKINENFRMKKIRVQLNLPIRSEQKQESLETHKTIEEDRKLLLQSAIVRIMKARRTLKHVVLVKETIDQIKSRFTPKVSDIKQCIDMLIEKEYLERQGRDEYIYLA skp1-pof10-4 Y2H showing interaction between skp1 and pof10 564982 N/A 564986 unspecified role unspecified role bait bait N/A 564987 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Pof10 was found to interact with Skp1. skp1-pof14 Y2H showing interaction between skp1 and pof14 564982 N/A 564988 unspecified role unspecified role prey prey N/A 564986 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table 1 Pof14 was found to interact with Skp1. skp1-zwf1 Y2H showing interaction between skp1 and zwf1 564982 N/A 564989 unspecified role unspecified role prey prey N/A 564986 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table 1 Zwf1 was found to interact with Skp1. skp1-pap1 Y2H showing interaction between skp1 and pap1 564982 N/A 564990 unspecified role unspecified role prey prey N/A 564986 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table 1 Pap1 was found to interact with Skp1. skp1-yrdi Y2H showing interaction between skp1 and yrdi 564982 N/A 564991 unspecified role unspecified role prey prey N/A 564986 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table 1 Yrdi (SPAC16E8.18) was found to interact with Skp1. skp1-pof6-3 Y2H showing interaction between skp1 and pof6 564982 N/A 564986 unspecified role unspecified role bait bait N/A 564992 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Pof6 was found to interact with Skp1. skp1-pof7-1 Y2H showing interaction between skp1 and pof7 564982 N/A 564986 unspecified role unspecified role bait bait N/A 564993 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Pof7 was found to interact with Skp1. skp1-mcm3 Y2H showing interaction between skp1 and mcm3 564982 N/A 564986 unspecified role unspecified role bait bait N/A 564994 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Mcm3 was found to interact with Skp1. skp1-pop2-1 Y2H showing interaction between skp1 and pop2 564982 N/A 564986 unspecified role unspecified role bait bait N/A 564995 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Pop2 was found to interact with Skp1. skp1-mrpl15 Y2H showing interaction between skp1 and mrpl15 564982 N/A 564986 unspecified role unspecified role bait bait N/A 564996 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Mrpl15 (SPBC1539.01c) was found to interact with Skp1. skp1-ral2 Y2H showing interaction between skp1 and ral2 564982 N/A 564997 unspecified role unspecified role prey prey N/A 564986 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table 1 Ral2 was found to interact with Skp1. skp1-alp7 Y2H showing interaction between skp1 and alp7 564982 N/A 564998 unspecified role unspecified role prey prey N/A 564986 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table 1 Alp7 was found to interact with Skp1. skp1-ybx1 Y2H showing interaction between skp1 and ybx1 564982 N/A 564986 unspecified role unspecified role bait bait N/A 564999 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Ybx1 (SPBC17D11.09) was found to interact with Skp1. skp1-pgk1 Y2H showing interaction between skp1 and pgk1 564982 N/A 564986 unspecified role unspecified role bait bait N/A 565000 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Pgk1 was found to interact with Skp1. skp1-lcf2 Y2H showing interaction between skp1 and lcf2 564982 N/A 565001 unspecified role unspecified role prey prey N/A 564986 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table 1 Lcf2 (SPBP4H10.11c) was found to interact with Skp1. skp1-yin3 Y2H showing interaction between skp1 and yin3 564982 N/A 564986 unspecified role unspecified role bait bait N/A 565002 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Yin3 (SPAC1705.03c) was found to interact with Skp1. skp1-ei2ba Y2H showing interaction between skp1 and ei2ba/ tif221 564982 N/A 565003 unspecified role unspecified role prey prey N/A 564986 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table 1 Ei2ba/tif221 was found to interact with Skp1. skp1-yfy2 Y2H showing interaction between skp1 and yfy2 564982 N/A 564986 unspecified role unspecified role bait bait N/A 565004 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Yfy2 (SPAC9.02c) was found to interact with Skp1. skp1-pmh1-2 Y2H showing interaction between skp1 and pmh1 564982 N/A 564986 unspecified role unspecified role bait bait N/A 565005 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table 1 Pmh1 was found to interact with Skp1. pof14-skp1 Y2H showing interaction between pof14 and skp1 564982 N/A 564986 unspecified role unspecified role prey prey N/A 564988 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table II, Figure 2A Skp1 was found to interact with Pof14. pof14-erg9 Y2H showing interaction between pof14 and erg9 564982 N/A 565006 unspecified role unspecified role prey prey N/A 564988 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table II, Figure 2A, 2B. Erg9 was found to interact with Pof14. pof14-gpm1 Y2H showing interaction between pof14 and gpm1 564982 N/A 565007 unspecified role unspecified role prey prey N/A 564988 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Table II Gpm1 was found to interact with Pof14. pof14-mug64 Y2H showing interaction between pof14 and mug64 564982 N/A 564988 unspecified role unspecified role bait bait N/A 565008 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table II Mug64 was found to interact with Pof14. pof14-tif32 Y2H showing interaction between pof14 and tif32 564982 N/A 564988 unspecified role unspecified role bait bait N/A 565009 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Table II Tif32 was found to interact with Pof14. pof14-skp1-2 Co-Ip showing interaction between skp1 and pof14 564983 N/A 564988 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast N/A 564986 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association false false false Figure 1D. Pof14 protein interacts with Skp1. pof14-cul1 Co-Ip showing interaction between cul1/pcu1 and pof14 564983 N/A 565010 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast N/A 564988 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association false false false Figure 1D. Pof14 protein interacts with Cul1/Pcu1. pof14-erg9-2 Co-IP showing interaction between pof14 and erg9 564983 N/A 564988 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast N/A 565006 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association false false false Figure 2C Erg9 was able to interact with Pof14 but not with Skp1. skp1-erg9 Co-IP showing lack of interaction between skp1 and erg9 564983 N/A 564986 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast N/A 565006 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association false false false Figure 2C Erg9 did not interact with Skp1. pof14-skp1-3 Co-Ip showing interaction between skp1 and pof14 564983 N/A 564986 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast N/A 564988 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association false false false Figure 3D. Pof14 protein interacts with Skp1 even under stress conditions (being exposed to 0.2mM H2O2/40mins). pof14-erg9-4 Co-IP showing interaction between pof14 and erg9 564983 N/A 565006 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast N/A 564988 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association false false false Supplementary Figure 1 Erg9 was able to interact with Pof14 under stress conditions (0.2mM H2O2, 40mins). skp1-pof14-1 Y2H showing interaction between skp1 and and intact pof14 564984 N/A 564988 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 564986 unspecified role unspecified role bait bait physical association physical association false false false Figure 1A, 1B Only the full-length Pof14 protein retained ability to interact with Skp1, indicating that an intact putative F-box is required for this binding. pof14-erg9-1 Y2H showing interaction between pof14 and erg9 564984 N/A 564988 unspecified role unspecified role bait bait N/A 565006 unspecified role unspecified role prey prey physical association physical association false false false Figure 2A, 2B. The C-terminal part of Erg9 was sufficient for interaction with Pof14. pof14-erg9-3 Colocalization of pof14 and erg9 564985 N/A 564988 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast N/A 565006 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association false false false Figure 2D Erg9 and Pof14 colocalized at vesicle-like and ER structures.