IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ song-2006b-4 Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). Plant Cell (1040-4651) 2006 song-2006b-1 Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). Plant Cell (1040-4651) 2006 song-2006b-2 Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. arath-leaf Arabidopsis thaliana leaf leaf leaf fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). Plant Cell (1040-4651) 2006 song-2006b-5 Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). Plant Cell (1040-4651) 2006 song-2006b-3 Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. arath-leaf Arabidopsis thaliana leaf leaf leaf molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). Plant Cell (1040-4651) 2006 epn1_arath Clathrin interactor EPSIN 1 At5g11710 EPSINR1 EPSIN-related 1 T22P22.100 EPSIN1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDFMKVFDQTVREIKREVNLKVLKVPEMEQKVLDATDNEPWGPHGTALAEIAQATKKFSECQMVMSVLWTRLSETGKDWRYVYKALAVIDYLISNGSERAVDEIIEHTYQISSLTSFEYVEPNGKDVGINVRKKAENIVALLNNKEKISEIRDKAVANRNKYVGLSSTGITYKSGSSASFGGSFQSGSSNFDSYKDRDSREDKNDYESFQKSRRGVKTEEQSYTSKKSFSRYGSTDHDNLSSGKKSPDSAKHRSYVSAAPSNNDDDFDDFDPRGTSSNKPSTGSANQVDLFGGDLIGDFLDSGPTETSSTNNNENFQEADLFADAAFVSASAQGAEFGSQTQKQVDLFSASEPSVTVSSAPPTVDLFASSESVVSPEAKISIPESMATPNIVDPFAAVPMDNFDGSDPFGAFTSHSASVSTGPQAPSVHGSATNTTSPLSFADSKPQHLQKKDPFQVKSGIWADSLSRGLIDLNITAPKKASLADVGVVGDLSNEDGNKASAASYYSGWSMGAGSGLGKTGLYSTQQQQQQQQQQQAPDISDDFFSSLSNQRYQSGGFKQ vti11_arath Vesicle transport v-SNARE 11 Vesicle transport v-SNARE protein VTI1a Vesicle soluble NSF attachment protein receptor VTI1a VTI11 VTI1A Protein SHOOT GRAVITROPISM 4 SGR4 ZIG ZIG1 MUL8_190 At5g39510 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSDVFDGYERQYCELSASLSKKCSSAISLDGEQKKQKLSEIKSGLENAEVLIRKMDLEARTLPPNLKSSLLVKLREFKSDLNNFKTEVKRITSGQLNAAARDELLEAGMADTKTASADQRARLMMSTERLGRTTDRVKDSRRTMMETEEIGVSILQDLHGQRQSLLRAHETLHGVDDNIGKSKKILTDMTRRMNKNKWTIGAIIIALIAAIFIILYFKLTK ap1g1_arath AP-1 complex subunit gamma-1 At1g23900 Adaptor protein complex AP-1 large subunit gamma-1 Clathrin assembly protein complex 1 gamma-1 large chain Gamma-adaptin 1 T23E23.7 GAMMA-ADR Adaptor-related protein complex 1 subunit gamma-1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNPFSSGTRLRDMIRAIRACKTAAEERAVVRKECADIRALINEDDPHDRHRNLAKLMFIHMLGYPTHFGQMECLKLIASPGFPEKRIGYLGLMLLLDERQEVLMLVTNSLKQDLNHSNQYVVGLALCALGNICSAEMARDLAPEVERLIQFRDPNIRKKAALCSTRIIRKVPDLAENFVNAAASLLKEKHHGVLITGVQLCYELCTINDEALEYFRTKCTEGLIKTLRDITNSAYQPEYDVAGITDPFLHIRLLRLLRVLGQGDADASDLMTDILAQVATKTESNKNAGNAVLYECVETIMAIEDTNSLRVLAINILGRFLSNRDNNIRYVALNMLMKAITFDDQAVQRHRVTILECVKDPDASIRKRALELVTLLVNENNVTQLTKELIDYLEISDEDFKEDLSAKICFIVEKFSPEKLWYIDQMLKVLCEAGKFVKDDVWHALIVVISNASELHGYTVRALYKSVLTYSEQETLVRVAVWCIGEYGDLLVNNVGMLGIEDPITVTESDAVDVIEDAITRHNSDSTTKAMALVALLKLSSRFPSISERIKDIIVKQKGSLLLEMQQRAIEYNSIVDRHKNIRSSLVDRMPVLDEATFNVRRAGSFPASVSTMAKPSVSLQNGVEKLPVAPLVDLLDLDSDDIMAAPSPSGTDFLQDLLGVDLGSSSAQYGATQAPKAGTDLLLDILSIGTPSPAQNSTSSIGLLSIADVNNNPSIALDTLSSPAPPHVATTSSTGMFDLLDGLSPSPSKEATNGPAYAPIVAYESSSLKIEFTFSKTPGNLQTTNVQATFTNLSPNTFTDFIFQAAVPKFLQLHLDPASSNTLLASGSGAITQNLRVTNSQQGKKSLVMRMRIGYKLNGKDVLEEGQVSNFPRGL ap2a1_arath AP-2 complex subunit alpha-1 At5g22770 Adaptor protein complex AP-2 subunit alpha-1 Alpha-adaptin 1 Clathrin assembly protein complex 2 alpha large chain 1 MDJ22.19 ALPHA-ADR Adaptor-related protein complex 2 subunit alpha-1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTGMRGLSVFISDVRNCQNKEAERLRVDKELGNIRTCFKNEKVLTPYKKKKYVWKMLYIHMLGYDVDFGHMEAVSLISAPKYPEKQVGYIVTSCLLNENHDFLKLAINTVRNDIIGRNETFQCLALTLVGNIGGRDFAESLAPDVQKLLISSSCRPLVRKKAALCLLRLFRKNPDAVNVDGWADRMAQLLDERDLGVLTSSTSLLVALVSNNHEAYSSCLPKCVKILERLARNQDVPQEYTYYGIPSPWLQVKAMRALQYFPTIEDPSTRKALFEVLQRILMGTDVVKNVNKNNASHAVLFEALSLVMHLDAEKEMMSQCVALLGKFISVREPNIRYLGLENMTRMLMVTDVQDIIKKHQSQIITSLKDPDISIRRRALDLLYGMCDVSNAKDIVEELLQYLSTAEFSMREELSLKAAILAEKFAPDLSWYVDVILQLIDKAGDFVSDDIWFRVVQFVTNNEDLQPYAASKAREYLDKIAIHETMVKVSAYILGEYGHLLARQPGCSASELFSILHEKLPTISTPTIPILLSTYAKLLMHAQPPDPELQKKVWAVFKKYESCIDVEIQQRAVEYFELSKKGPAFMDVLAEMPKFPERQSSLIKKAENVEDTADQSAIKLRAQQQPSNAMVLADQQPVNGAPPPLKVPILSGSTDPESVARSLSHPNGTLSNIDPQTPSPDLLSDLLGPLAIEAPPGAVSNEQHGPVGAEGVPDEVDGSAIVPVEEQTNTVELIGNIAERFHALCLKDSGVLYEDPHIQIGIKAEWRGHHGRLVLFMGNKNTSPLTSVQALILPPAHLRLDLSPVPDTIPPRAQVQSPLEVMNIRPSRDVAVLDFSYKFGANVVSAKLRIPATLNKFLQPLQLTSEEFFPQWRAISGPPLKLQEVVRGVRPLALPEMANLFNSFHVTICPGLDPNPNNLVASTTFYSESTGAILCLARIETDPADRTQLRMTVGTGDPTLTFELKEFIKEQLITVPMGSRALVPAAGPAPPVAQPPSPAALADDPGAMLAGLL vsr1_arath Vacuolar-sorting receptor 1 Epidermal growth factor receptor-like protein 1 BP80-like protein b Spot 3 protein VSR1 BP80B ELP ELP1 At3g52850 F8J2.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLGLFTLSFLLILNLAMGRFVVEKNNLKVTSPDSIKGIYECAIGNFGVPQYGGTLVGTVVYPKSNQKACKSYSDFDISFKSKPGRLPTFVLIDRGDCYFTLKAWIAQQAGAAAILVADSKAEPLITMDTPEEDKSDADYLQNITIPSALITKTLGDSIKSALSGGDMVNMKLDWTESVPHPDERVEYELWTNSNDECGKKCDTQIEFLKNFKGAAQILEKGGHTQFTPHYITWYCPEAFTLSKQCKSQCINHGRYCAPDPEQDFTKGYDGKDVVVQNLRQACVYRVMNDTGKPWVWWDYVTDFAIRCPMKEKKYTKECADGIIKSLGIDLKKVDKCIGDPEADVENPVLKAEQESQIGKGSRGDVTILPTLVVNNRQYRGKLEKGAVLKAMCSGFQESTEPAICLTEDLETNECLENNGGCWQDKAANITACRDTFRGRLCECPTVQGVKFVGDGYTHCKASGALHCGINNGGCWRESRGGFTYSACVDDHSKDCKCPLGFKGDGVKNCEDVDECKEKTVCQCPECKCKNTWGSYECSCSNGLLYMREHDTCIGSGKVGTTKLSWSFLWILIIGVGVAGLSGYAVYKYRIRSYMDAEIRGIMAQYMPLESQPPNTSGHHMDI clah1_arath Clathrin heavy chain 1 CHC1 F9F8.6 F11B9.30 At3g11130 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAAANAPIIMKEVLTLPSVGIGQQFITFTNVTMESDKYICVRETAPQNSVVIIDMNMPMQPLRRPITADSALMNPNSRILALKAQVPGTTQDHLQIFNIEAKAKLKSHQMPEQVAFWKWITPKMLGLVTQTSVYHWSIEGDSEPVKMFDRTANLANNQIINYKCSPNEKWLVLIGIAPGSPERPQLVKGNMQLFSVDQQRSQALEAHAASFAQFKVPGNENPSILISFASKSFNAGQITSKLHVIELGAQPGKPSFTKKQADLFFPPDFADDFPVAMQVSHKFNLIYVITKLGLLFVYDLETASAIYRNRISPDPIFLTSEASSVGGFYAINRRGQVLLATVNEATIIPFISGQLNNLELAVNLAKRGNLPGAENLVVQRFQELFAQTKYKEAAELAAESPQGILRTPDTVAKFQSVPVQAGQTPPLLQYFGTLLTRGKLNSYESLELSRLVVNQNKKNLLENWLAEDKLECSEELGDLVKTVDNDLALKIYIKARATPKVVAAFAERREFDKILIYSKQVGYTPDYMFLLQTILRTDPQGAVNFALMMSQMEGGCPVDYNTITDLFLQRNLIREATAFLLDVLKPNLPEHAFLQTKVLEINLVTFPNVADAILANGMFSHYDRPRVAQLCEKAGLYIQSLKHYSELPDIKRVIVNTHAIEPQALVEFFGTLSSEWAMECMKDLLLVNLRGNLQIIVQACKEYCEQLGVDACIKLFEQFKSYEGLYFFLGSYLSMSEDPEIHFKYIEAAAKTGQIKEVERVTRESNFYDAEKTKNFLMEAKLPDARPLINVCDRFGFVPDLTHYLYTNNMLRYIEGYVQKVNPGNAPLVVGQLLDDECPEDFIKGLILSVRSLLPVEPLVAECEKRNRLRLLTQFLEHLVSEGSQDVHVHNALGKIIIDSNNNPEHFLTTNPYYDSKVVGKYCEKRDPTLAVVAYRRGQCDEELINVTNKNSLFKLQARYVVERMDGDLWEKVLTEENEYRRQLIDQVVSTALPESKSPEQVSAAVKAFMTADLPHELIELLEKIVLQNSAFSGNFNLQNLLILTAIKADPSRVMDYINRLDNFDGPAVGEVAVDAQLYEEAFAIFKKFNLNVQAVNVLLDNVRSIERAVEFAFRVEEDAVWSQVAKAQLREGLVSDAIESFIRADDTTQFLEVIRASEDTNVYDDLVRYLLMVRQKVKEPKVDSELIYAYAKIERLGEIEEFILMPNVANLQHVGDRLYDEALYEAAKIIYAFISNWAKLAVTLVKLQQFQGAVDAARKANSAKTWKEVCFACVDAEEFRLAQICGLNIIIQVDDLEEVSEYYQNRGCFNELISLMESGLGLERAHMGIFTELGVLYARYRYEKLMEHIKLFSTRLNIPKLIRACDEQQHWQELTYLYIQYDEFDNAATTVMNHSPEAWEHMQFKDIVAKVANVELYYKAVHFYLQEHPDIINDLLNVLALRLDHTRVVDIMRKAGHLRLIKPYMVAVQSNNVSAVNEALNEIYAEEEDYDRLRESIDLHDSFDQIGLAQKIEKHELVEMRRVAAYIYKKAGRWKQSIALSKKDNMYKDCMETASQSGDHDLAEQLLVYFIEQGKKECFATCLFVCYDLIRPDVALELAWINNMIDFAFPYLLQFIREYSGKVDELIKDKLEAQKEVKAKEQEEKDVMSQQNMYAQLLPLALPAPPMPGMGGGGYGPPPQMGGMPGMSGMPPMPPYGMPPMGGY espin1-vsti11 566518 N/A 566523 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 566524 unspecified role unspecified role prey prey c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 5C Q9SEL6 transformation espin1-at1g23900 566518 N/A 566523 unspecified role unspecified role bait bait c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 566525 unspecified role unspecified role prey prey n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 6A Q84K16 transformation at1g23900-espin1 566518 N/A 566525 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 566523 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 6B The authors state that the Putative gamma-adaptin , Q84K16, was expressed as the 'ear domain' of the protein tagged with the maltose binding protein epitope. The region of the ear domain was not given and could not be established. Q8VY07; Q84K16 transformation at5g22770-espin1 566518 N/A 566526 unspecified role unspecified role bait bait c terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 566523 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 6B The authors state that the Putative alpha-adaptin , Q8LPL6, was expressed as the 'ear domain' of the protein tagged with the maltose binding protein epitope. The region of the ear domain was not given and could not be established. The authors describe the interaction between Q8LPL6 and Q8VY07 as 'weak'. Q8VY07; Q8LPL6 transformation espin1-vsr1 566518 N/A 566523 unspecified role unspecified role bait bait c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 566527 unspecified role unspecified role prey prey c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 7 P93026 transformation espin1-clathrin 566519 N/A 566528 unspecified role unspecified role prey prey N/A 566523 unspecified role unspecified role bait bait c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 4A 4B 4C The identity of the protein pulled down by GST:ESPIN1 was assumed to be clathrin since , as the authors state, "GST:ESPIN1, but not GST alone, precipitated from plant extracts a 180kD protein species that was recognised by anti-clathrin antibody(Figure 4C), indicating that ESPIN1 bound clathrin." clathrin-espin1 566519 N/A 566523 unspecified role unspecified role prey prey N/A 566528 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q0WNJ6:p.Leu470Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 4A 4B 4C The identity of the protein pulled down by GST:ESPIN1 was assumed to be clathrin since , as the authors state, "GST:ESPIN1, but not GST alone, precipitated from plant extracts a 180kD protein species that was recognised by anti-clathrin antibody(Figure 4C), indicating that ESPIN1 bound clathrin." epsin1-vt11 566520 N/A 566524 unspecified role unspecified role neutral component neutral component c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 566523 unspecified role unspecified role neutral component neutral component c terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false 3Bm 3Bn 3Bo Q8VY07; Q9SEL6 transfection espin1-clathrin-1 566520 N/A 566528 unspecified role unspecified role neutral component neutral component N/A 566523 unspecified role unspecified role neutral component neutral component n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 4D Q8VY07 transfection vsti11-espin1 566521 N/A 566523 unspecified role unspecified role prey prey c terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 566524 unspecified role unspecified role bait bait c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 5 Q9SEL6; Q8VY07 transformation espin1-vsr1-1 566521 N/A 566523 unspecified role unspecified role prey prey c terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 566527 unspecified role unspecified role bait bait c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 7 P93026; Q8VY07 transformation clathrin-vsr1 566522 N/A 566527 unspecified role unspecified role neutral component neutral component N/A 566528 unspecified role unspecified role neutral component neutral component physical association physical association false false false S1 Clathrin and VSR1 eluted between 443 and 669kD.