IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ laubinger-2006-1 Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight identificat confirmation by molecular weight weight identificat Laubinger S., Marchal V., Le Gourrierec J., Gentilhomme J., Wenkel S., Adrian J., Jang S., Kulajta C., Braun H., Coupland G., Hoecker U. Proteins were identified using references given in the paper. Prey proteins were fully labeled with 35Smethionine, while bait proteins were synthesized in the presence of a mixture of labeled and unlabelled methionine in order to facilitate protein detection after SDS-PAGE visualized using a phosphorimager. hoeckeru@uni-koln.de Development (0950-1991) 2006 Only protein-protein interactions imex curation laubinger-2006-2 Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability. arath-leaf Arabidopsis thaliana leaf leaf leaf confocal microscopy confocal microscopy predetermined predetermined participant predetermined Laubinger S., Marchal V., Le Gourrierec J., Gentilhomme J., Wenkel S., Adrian J., Jang S., Kulajta C., Braun H., Coupland G., Hoecker U. Proteins were identified using references given in the paper. Prey proteins were fully labeled with 35Smethionine, while bait proteins were synthesized in the presence of a mixture of labeled and unlabelled methionine in order to facilitate protein detection after SDS-PAGE visualized using a phosphorimager. hoeckeru@uni-koln.de Development (0950-1991) 2006 Only protein-protein interactions imex curation laubinger-2006-3 Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability. arath-leaf Arabidopsis thaliana leaf leaf leaf fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Laubinger S., Marchal V., Le Gourrierec J., Gentilhomme J., Wenkel S., Adrian J., Jang S., Kulajta C., Braun H., Coupland G., Hoecker U. Proteins were identified using references given in the paper. Prey proteins were fully labeled with 35Smethionine, while bait proteins were synthesized in the presence of a mixture of labeled and unlabelled methionine in order to facilitate protein detection after SDS-PAGE visualized using a phosphorimager. hoeckeru@uni-koln.de Development (0950-1991) 2006 Only protein-protein interactions imex curation col1_arath Zinc finger protein CONSTANS-LIKE 1 COL1 At5g15850 F14F8_230 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLKVESNWAQACDTCRSAACTVYCRADSAYLCSSCDAQVHAANRLASRHERVRVCQSCERAPAAFFCKADAASLCTTCDSEIHSANPLARRHQRVPILPISEYSYSSTATNHSCETTVTDPENRLVLGQEEEDEDEAEAASWLLPNSGKNSGNNNGFSIGDEFLNLVDYSSSDKQFTDQSNQYQLDCNVPQRSYGEDGVVPLQIEVSKGMYQEQQNFQLSINCGSWGALRSSNGSLSHMVNVSSMDLGVVPESTTSDATVSNPRSPKAVTDQPPYPPAQMLSPRDREARVLRYREKKKMRKFEKTIRYASRKAYAEKRPRIKGRFAKKKDVDEEANQAFSTMITFDTGYGIVPSF spa1_arath Protein SUPPRESSOR OF PHYA-105 1 SPA1 F11C10.3/F11C10.4 At2g46340/At2g46350 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPVMERVAEETVATNNIQLKARVDDVPCNKLDARHNDMVIQSETANSDCPGSSAHRNVDLTKPPPPEEAAGAKLSVEELTLGNYRIVQGSNNTNVDSPRAGKFEHLYRLARGSAFRAGDGDLDSQPRDMDQMLSRIRQQLAGAPSERQNLKPFMSRRSDQNLEAFSERLRAAGENSIMNAPALISEGVQMKTPVSSSNFSQLLLKRAMKGKGVVGKNQETPPEFVSDQDLGSKEKKLDISKSPTPHDVLPLKSSPKGNGMVSHGDGNHSKSSIGISLREFLRSSYAKREKRHGLCLFRQLVELVDSAHSKRLFLLDLRPSLFTLVPSKKLRYIGNFGKNDLESDVDEDLNRRRPVVEESSSGGRDSKKRKMDLHLNSPGNQLQATSTGRPFKRKSPVIDLNMVDARNPDSCELQQQDYIKNLSVSSVSRKQSMSTWLEEQWYTCPEEINGEDIGEKSNIYALGVLLFELLCHCESGEMHAAMMADLRHRILPPTFLSKYPKEAGFCLWLLHPEPSSRPSARDILKSELICEDDSVKSTAAAEEISELLLHFLSSLEVQKKKKASKLLQDIQTLEDDIKEAERRYSSNVSLVRSHGAIEKRVQSSPLDEHCTTSSALFVPTANTDRLMSNIRQLEDAYFFMRSQINLSSSAATARSDKTLKDRDRCSENQNENQDMSTKGKSSDQLEVFFEGLCKFARYSKFETCGTIRSGDLLNSASVVCSLSFDPDEEHIAAAGISKKIKIFDFNAFMNESVGVHYPLVEMVNKSKLSCVCWNSYIKNYLASTDYDGVVQIWDAGTGQGFSQYTEHQKRAWSVDFSPSDPTKFVSGSDDCSVKLWSINEKRSLGTIWSPANVCCVQFSSYSNHLLAFGSADYKVYCYDLRYVKTPWCTLAGHEKAVSYVKFMDSETIVSASTDNSLKLWNLNKTNSSGLSPGACSLTYKGHTNQKNFVGLSVLDGYIACGSETNEVYSYYKSLPMPMTSYKFGSVDPISGNEYFDDNGQFVSSVCWRKKSNMLVAANSTGNMKLLKLV spa2_arath Protein SPA1-RELATED 2 SPA2 F2P3.13 F2P3.14 T22B4.90 At4g11110 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMDEGSVGDVSRIDEADVAHLQFKNSEQSFKPENIEVREVKEVQVQREAGSPDCSYGVIADFLDGKNGGDHVELIGNEPCSSRQNTNDEGDVVEELTVKTCEGSSMAIVGRPSSRARLEMNRSQFLHRFPLDGDLPGSSSMSKKVIDRGTVSILRNAGKMSLPETSNGQLAIIAVNGEANEHLTNVERNPVPVEALSHEGIKTKMLSQSGFSQFFVRKTLKGKGVTFRGPPNNRSKARNMDQQTVASSGSALVIANTSAKISSSIPLAAYDGLPCLPSNTSKPSSCANPSDTHRGCGGEGLSLREWLKSERQEVNKAECMYIFRQIVDHVDCSHSQGVVLCDLRPSSFKIFKENAVKYVVSGSQRESFDSNMNKETLSQLENPLVRRRLGDTSSLSIPAKKQKSSGPSSRQWPMFQRAGGVNIQTENNDGAIQEFHFRSSQPHCSTVACPFTSVSEQLEEKWYASPEELRGDMRSASSNIYSLGILLYELLSQFQCERAREAAMSDIRHRILPPKFLSENPKEAGFCLWLLHPESSCRPSTRDILQSEVVNGIPDLYAEGLSLSIEQEDTESELLQHFLFLSQEKRQKHAGNLMEEIASVEADIEEIVKRRCAIGPPSLEEASSSSPASSVPEMRLIRNINQLESAYFAARIDAHLPEARYRLRPDRDLLRNSDNTVAEVENSETWSSDDRVGAFFDGLCKYARYSKFETRGVLRTSELNNTSNVICSLGFDRDEDYFATAGVSKKIKIYEFNSLFNESVDIHYPAIEMPNRSKLSGVCWNNYIRNYLASSDYDGIVKLWDVTTGQAISHFIEHEKRAWSVDFSEACPTKLASGSDDCSVKLWNINERNCLGTIRNIANVCCVQFSPQSSHLLAFGSSDFRTYCYDLRNLRTPWCILSGHNKAVSYAKFLDNETLVTASTDNTLKLWDLKKTTHGGLSTNACSLTFGGHTNEKNFVGLSTSDGYIACGSETNEVYAYHRSLPMPITSYKFGSIDPISGKEIEEDNNLFVSSVCWRKRSNMVVSASSNGSIKVLQLV spa3_arath Protein SPA1-RELATED 3 SPA3 K7L4.16 At3g15354 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEGSSNSNSRGFNTSGVSDRNTEFLPVERLTTRSKPSSHVDEYVRSLFGSTSTHKSGEDDSLGIDPFVRSLEWGDVSLRQWLDKPERSVDVFECLHVFRQIVEIVNAAHSQGIVVHNVRPSCFVMSSFNHVSFIESASCSDSGSDSLEDGPISQKEIGSSRREEAVSKAIAIEEKGVYNKLLERKIEKLEEEKTQPFPMKHILAMETSWYTSPEEDFGSSSTCASDVYRLGVLLFELFCPVPSREEKSRTMSSLRHRVLPPQILLKCPKEASFCLWLLHPEPTCRPSMSDLLQSEFITEPRDNLEEREAAIELRDRIEEQESLLEFLLLIQQRKQESAYRLQDTVSLLSSDIEQVVKRQLILKKRGSSLSDFSKDDHQYTSGQPLMSFQANEEPSAFLASRKRVRQGILALENGVEVDEESQGSTLLESSRLMRNFKKLESVYFLTRRRQMKAAASGKSLTRHSPLSSENGRGSMIVSEKSSVSNPVAPKAFFNNDSRQGGWIDPFLEGLCRYLSFSQLRVKADLKQGDLLNSSNLVCALAFDREGELFATAGVNKKIKIFECNSIVNDNRDIHYPVVELAGRSKLSSLCWNSYIKSQIASSNFDGVVQIWDVARSQLVTEMKEHKKRVWSIDISSADPTLLASGSDDGTVKLWSINQGVSIGTIKTKANVCCVQFPSDSGRSLAFGSADHKVYYYDLRNPKIPLCTMIGHSKTVSYVKFVDSSTLVSSSTDNTLKLWDLSMSASGINESPLHSFTGHTNLKNFVGLSVSDGYIATGSETNEVFVYHKAFPMPVMSYMFNNTDSMSGLEVDDASQFISSICWRGQSSTLVAANSNGNIKILEMMT spa4_arath Protein SPA1-RELATED 4 SPA4 At1g53090 F8L10.5 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKGSSESSSRGLNNTSGVSEFCTDGSKSLSHIDYVRSLLGSHKEANLGGLDDDSIVRALECEDVSLRQWLDNPDRSVDAFECFHVFRQIVEIVNAAHSQGIVVHNVRPSCFVMSSFNNVSFIESASCSDSGSDEDATTKSREIGSSRQEEILSERRSKQQEEVKKQPFPMKQILAMEMSWYTSHEEDNGSLCNCASDIYRLGVLLFELFCPVSSREEKSRTMSSLRHRVLPPQILLNWPKEASFCLWLLHPEPSCRPSMSELLQSEFINEPRENLEEREAAMELRDRIEEQELLLEFLFLIQQRKQEAADKLQDTISLLSSDIDQVVKRQLVLQQKGRDVRSFLASRKRIRQGAETTAAEEENDDNSIDEESKLDDTLESTLLESSRLMRNLKKLESVYFATRYRQIKAATAAEKPLARYYSALSCNGRSSEKSSMSQPSKDPINDSRQGGWIDPFLEGLCKYLSFSKLRVKADLKQGDLLNSSNLVCAIGFDRDGEFFATAGVNKKIKIFECESIIKDGRDIHYPVVELASRSKLSGICWNSYIKSQVASSNFEGVVQVWDVARNQLVTEMKEHEKRVWSIDYSSADPTLLASGSDDGSVKLWSINQGVSIGTIKTKANICCVQFPSETGRSLAFGSADHKVYYYDLRNPKLPLCTMIGHHKTVSYVRFVDSSTLVSSSTDNTLKLWDLSMSISGINETPLHSFMGHTNVKNFVGLSVSDGYIATGSETNEVFVYHKAFPMPVLSYKFKTIDPVSELEVDDASQFISSVCWRGQSSTLVAANSTGNIKILEMV spa1-co 546710 N/A 546713 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 546714 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false gad tag GAL4 activation domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 6B 6C The UniProt entry for CO protein has a length of 355 amino acids. However the paper describes the binding region of CO to SPA1 as being from amino acid residue 272 to residue 373. The UniProt coordinates were used to delimit the binding region of the CO protein to the SPA1 protein. spa2-co 546710 N/A 546713 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 546715 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false gad tag GAL4 activation domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 5A spa3-co 546710 N/A 546713 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 546716 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false gad tag GAL4 activation domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 5A spa4-co 546710 N/A 546717 unspecified role unspecified role bait bait gad tag GAL4 activation domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 546713 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 5A spa1-co-2 546711 N/A 546713 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 546714 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 5B The proteins colocalised to the nucleus. spa1-co-1 546712 N/A 546714 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 546713 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 5C 5D