DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yang-2006-2 Fibrillin expression is regulated by abscisic acid response regulators and is involved in abscisic acid-mediated photoprotection. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yang Y., Sulpice R., Himmelbach A., Meinhard M., Christmann A., Grill E. Directed yeast two hybrid assay used in this study. erwin.grill@wzw.tum.de arabidopsis cDNA libraries used. Source of cDNA not cited. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 IMEx Only protein-protein interactions imex curation yang-2006-1 Fibrillin expression is regulated by abscisic acid response regulators and is involved in abscisic acid-mediated photoprotection. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yang Y., Sulpice R., Himmelbach A., Meinhard M., Christmann A., Grill E. erwin.grill@wzw.tum.de arabidopsis cDNA libraries used. Source of cDNA not cited. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 IMEx Only protein-protein interactions imex curation yang-2006-3 Fibrillin expression is regulated by abscisic acid response regulators and is involved in abscisic acid-mediated photoprotection. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yang Y., Sulpice R., Himmelbach A., Meinhard M., Christmann A., Grill E. erwin.grill@wzw.tum.de arabidopsis cDNA libraries used. Source of cDNA not cited. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 IMEx Only protein-protein interactions imex curation yang-2006-4 Fibrillin expression is regulated by abscisic acid response regulators and is involved in abscisic acid-mediated photoprotection. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Yang Y., Sulpice R., Himmelbach A., Meinhard M., Christmann A., Grill E. Binding of radio-labelled PAP1 detected by autoradiography. erwin.grill@wzw.tum.de arabidopsis cDNA libraries used. Source of cDNA not cited. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 IMEx Only protein-protein interactions imex curation pap1_arath Probable plastid-lipid-associated protein 1, chloroplastic PAP1 FBN1a FIB1a Fibrillin-1a PGL35 Plastoglobulin 35 T24H24.16 At4g04020 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATVPLFTQFPCKTLNPSSSNTKHQSKSPILLPINSINRRSEIGVSVHRPDFKIRATDIDDEWGQDGVERVFASSSTVSVADKAIESVEETERLKRSLADSLYGTDRGLSVSSDTRAEISELITQLESKNPTPAPNEALFLLNGKWILAYTSFVGLFPLLSRRIEPLVKVDEISQTIDSDSFTVQNSVRFAGPFSTTSFSTNAKFEIRSPKRVQIKFEQGVIGTPQLTDSIEIPESVEVLGQKIDLNPIKGLLTSVQDTASSVARTISNQPPLKFSLPSDNTQSWLLTTYLDKDLRISRGDGGSVYVLIKEGSSLLNP p2c77_arath Protein phosphatase 2C 77 Protein ABSCISIC ACID-INSENSITIVE 2 ABI2 At5g57050 MHM17.19 Protein phosphatase 2C ABI2 PP2C77 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDEVSPAVAVPFRPFTDPHAGLRGYCNGESRVTLPESSCSGDGAMKDSSFEINTRQDSLTSSSSAMAGVDISAGDEINGSDEFDPRSMNQSEKKVLSRTESRSLFEFKCVPLYGVTSICGRRPEMEDSVSTIPRFLQVSSSSLLDGRVTNGFNPHLSAHFFGVYDGHGGSQVANYCRERMHLALTEEIVKEKPEFCDGDTWQEKWKKALFNSFMRVDSEIETVAHAPETVGSTSVVAVVFPTHIFVANCGDSRAVLCRGKTPLALSVDHKPDRDDEAARIEAAGGKVIRWNGARVFGVLAMSRSIGDRYLKPSVIPDPEVTSVRRVKEDDCLILASDGLWDVMTNEEVCDLARKRILLWHKKNAMAGEALLPAEKRGEGKDPAAMSAAEYLSKMALQKGSKDNISVVVVDLKGIRKFKSKSLN ab12-pap1-1 Directed yeast 2 hybrid analysis reveals interaction between AB12 protein and PAP1 (Fibrillin) protein 493278 N/A 493282 unspecified role unspecified role prey prey N/A 493283 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O04719:p.Gly168Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 1 A ab12-pap1-3 Directed yeast 2 hybrid analysis reveals interaction between AB12 protein and PAP1 (Fibrillin) protein 493278 N/A 493283 unspecified role unspecified role bait bait N/A 493282 unspecified role unspecified role prey prey n-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Control directed two hybrid study mentioned in text but not shown in a figure. ab12-pap1-4 Yeast 2 hybrid analysis reveals interaction between AB12 protein and PAP1 (Fibrillin) protein 493279 N/A 493283 unspecified role unspecified role bait bait N/A 493282 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false pap1-ab12-1 Pulldown analysis reveals interaction between AB12 protein and PAP1 (Fibrillin) protein 493280 N/A 493283 unspecified role unspecified role bait bait c-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 493282 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 1 B. ab12-pap1-2 Pulldown analysis reveals interaction between AB12 protein and PAP1 (Fibrillin) protein 493281 N/A 493282 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 493283 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O04719:p.Gly168Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 1 C.