IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ betschinger-2006-1 Asymmetric segregation of the tumor suppressor brat regulates self-renewal in Drosophila neural stem cells. drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom ms participant Identification by mass spectrometry ms participant Betschinger J., Mechtler K., Knoblich JA. TAP tagged constructs were expressed in 4-7 hr embryos and embryonic extracts were prepared 45 minutes after recovery. 1A knoblich@imba.oeaw.ac.at Cell (0092-8674) 2006 Only protein-protein interactions 2 imex curation betschinger-2006-2 Asymmetric segregation of the tumor suppressor brat regulates self-renewal in Drosophila neural stem cells. drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Betschinger J., Mechtler K., Knoblich JA. TAP tagged constructs were expressed in 4-7 hr embryos and embryonic extracts were prepared 45 minutes after recovery. 1A knoblich@imba.oeaw.ac.at Cell (0092-8674) 2006 Only protein-protein interactions 2 imex curation pros_drome Homeobox protein prospero pros CG17228 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMSSEEYEADCFGLYSDENNVLLKANEPETTAATKQQHQPQFQQQQQQQQQQQQHHHQHQQPPPSSNGHTSPIPSQVNGDGSAANCGESGKTNTNTGSHSHSNSGNTNTDADKEQEREKAKEKANEEESEDSDDDVVVVLEGCEGNASSSSSSSNSNSNASSNNHKAAATTTTTATTANHCNRSGGSSRSHRSARSSRQISQSTAVGKTTTCAAKKPTAAQATTTTAKNSNSNSNVNVNVNVNSNGSGNGNANSKVNRRSRQRSLSKDINNQPASSNSNSNSSNNSSNSNGGATATAAGFMSSAAAAAAGAAGGGALFQPQSVSTANSSSSNNNNSSTPAALATHSPTSNSPVSGASSASSLLTAAFGNLFGGSSAKMLNELFGRQMKQAQDATSGLPQSLDNAMLAAAMETATSAELLIGSLNSTSKLLQQQHNNNSIAPANSTPMSNGTNASISPGSAHSSSHSHQGVSPKGSRRVSACSDRSLEAAAADVAGGSPPRAASVSSLNGGASSGEQHQSQLQHDLVAHHMLRNILQGKKELMQLDQELRTAMQQQQQQLQEKEQLHSKLNNNNNNNIAATANNNNNTTMESINLIDDSEMADIKIKSEPQTAPQPQQSPHGSSHSSRSGSGSGSHSSMASDGSLRRKSSDSLDSHGAQDDAQDEEDAAPTGQRSESRAPEEPQLPTKKESVDDMLDEVELLGLHSRGSDMDSLASPSHSDMMLLDKDDVLDEDDDDDCVEQKTSGSGCLKKPGMDLKRARVENIVSGMRCSPSSGLAQAGQLQVNGCKKRKLYQPQQHAMERYVAAAAGLNFGLNLQSMMLDQEDSESNELESPQIQQKRVEKNALKSQLRSMQEQLAEMQQKYVQLCSRMEQESECQELDQDQDVEQEQEPDNGSSDHIELSPSPTLTGDGDVSPNHKEETGQERPGSSSPSPSPLKPKTSLGESSDSGANMLSQMMSKMMSGKLHNPLVGVGHPALPQGFPPLLQHMGDMSHAAAMYQQFFFEQEARMAKEAAEQQQQQQQQQQQQQQQQQQEQQRRFEQEQQEQQRRKEEQQQQIQRQQQHLQQLQQQQMEQQHVATAAPRPQMHHPAPARLPTRMGGAAGHTALKSELSEKFQMLRANNNSSMMRMSGTDLEGLADVLKSEITTSLSALVDTIVTRFVHQRRLFSKQADSVTAAAEQLNKDLLLASQILDRKSPRTKVADRPQNGPTPATQSAAAMFQAPKTPQGMNPVAAAALYNSMTGPFCLPPDQQQQQQTAQQQQSAQQQQQSSQQTQQQLEQNEALSLVVTPKKKRHKVTDTRITPRTVSRILAQDGVVPPTGGPPSTPQQQQQQQQQQQQQQQQQQQQASNGGNSNATPAQSPTRSSGGAAYHPQPPPPPPPMMPVSLPTSVAIPNPSLHESKVFSPYSPFFNPHAAAGQATAAQLHQHHQQHHPHHQSMQLSSSPPGSLGALMDSRDSPPLPHPPSMLHPALLAAAHHGGSPDYKTCLRAVMDAQDRQSECNSADMQFDGMAPTISFYKQMQLKTEHQESLMAKHCESLTPLHSSTLTPMHLRKAKLMFFWVRYPSSAVLKMYFPDIKFNKNNTAQLVKWFSNFREFYYIQMEKYARQAVTEGIKTPDDLLIAGDSELYRVLNLHYNRNNHIEVPQNFRFVVESTLREFFRAIQGGKDTEQSWKKSIYKIISRMDDPVPEYFKSPNFLEQLE brat_drome Brain tumor protein brat CG10719 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASSPTPSLDSMRGGANSIESYEHAGYLSDSPLTLSGSSPPASDSAICSDEYTGGSSVKSRSEVTVINGHHPISASVSSSSSASSSSCSSSSSSSSSSSSSSSSTSGLSGCGSTSSSVISANNVASSNGPGVIGSNLQSSNNGGNSGISSLVVGAGKGSNSSSNSSSSNTSANGSPPRCTACKSKCSDAVAKCFECQSYLCANCVTAHEFMHCFNGHNVCLIKGFEASTTGTPLSVGSPSNNPASNEFKYASSLTMMLQQQQQLDSQQQQQQQQLLPAQPMSQLSKIVLAAAAQANSQEQQREDSIYGSLHPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQSPGMTTSTGSTANESALQTLLADMRGKIGEIVGIAGNSDQNLTKVKLQYQKAHNELNETHQFFASMLDERKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVERSPAPPSSLLTEALLLRKSLEQQLQTGIQEMQLPFEIEFMSNYQSIQAGVRNTFGYIRANSSDGGPTGMSLTSNGHGKQPPIARPTQSASNSSASSAGSGHHGHHQQSHHHGHHNHHQTAHHQQLQAQSSLHGLGLGLSGASLLDSSSSAGGAVGAFSNGGLLLGGRDRNALAVEQHFGELMPKRGGGGYTGSNGSATSAVAHYNPYEKWSNGGSDNLFSSVTSGVSGSSAVADAFASLSAVGGSVVSGAGAGGSTVSSESLLDLTNKLLSATIYPPKSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNAQNDIIVADTNNHRIQIFDKEGRFKFQFGECGKRDSQLLYPNRVAVVRNSGDIIVTERSPTHQIQIYNQYGQFVRKFGATILQHPRGVTVDNKGRIIVVECKVMRVIIFDQNGNVLHKFGCSKHLEFPNGVVVNDKQEIFISDNRAHCVKVFNYEGQYLRQIGGEGITNYPIGVGINSNGEILIADNHNNFNLTIFTQDGQLISALESKVKHAQCFDVALMDDGSVVLASKDYRLYIYRYVQLAPVGM stau_drome Maternal effect protein staufen stau CG5753 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQHNVHAARPAPHIRAAHHHSHSHAHMHLHPGMEQHLGPSLQQQQQPPPPPQQPPHRDLHARLNHHHLHAQQQQQQQTSSNQAGAVAAAGAAYHHGNINSNSGSNISSNSNQMQKIRQQHQHLSSSNGLLGNQPPGPPPQAFNPLAGNPAALAYNQLPPHPPHHMAAHLGSYAAPPPHYYMSQAKPAKYNHYGSNANSNSGSNNSNSNYAPKAILQNTYRNQKVVVPPVVQEVTPVPEPPVTTNNATTNSTSNSTVIASEPVTQEDTSQKPETRQEPASADDHVSTGNIDATGALSNEDTSSSGRGGKDKTPMCLVNELARYNKITHQYRLTEERGPAHCKTFTVTLMLGDEEYSADGFKIKKAQHLAASKAIEETMYKHPPPKIRRSEEGGPMRTHITPTVELNALAMKLGQRTFYLLDPTQIPPTDSIVPPEFAGGHLLTAPGPGMPQPPPPPAYALRQRLGNGFVPIPSQPMHPHFFHGPGQRPFPPKFPSRFALPPPLGAHVHHGPNGPFPSVPTPPSKITLFVGKQKFVGIGRTLQQAKHDAAARALQVLKTQAISASEEALEDSMDEGDKKSPISQVHEIGIKRNMTVHFKVLREEGPAHMKNFITACIVGSIVTEGEGNGKKVSKKRAAEKMLVELQKLPPLTPTKQTPLKRIKVKTPGKSGAAAREGSVVSGTDGPTQTGKPERRKRLNPPKDKLIDMDDADNPITKLIQLQQTRKEKEPIFELIAKNGNETARRREFVMEVSASGSTARGTGNSKKLAKRNAAQALFELLEAVQVTPTNETQSSEECSTSATMSAVTAPAVEATAEGKVPMVATPVGPMPGILILRQNKKPAKKRDQIVIVKSNVESKEEEANKEVAVAAEENSNNSANSGDSSNSSSGDSQATEAASESALNTSTGSNTSGVSSNSSNVGANTDGNNHAESKNNTESSSNSTSNTQSAGVHMKEQLLYLSKLLDFEVNFSDYPKGNHNEFLTIVTLSTHPPQICHGVGKSSEESQNDAASNALKILSKLGLNNAMK o44224_drome mira CG12249 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSFSKAKLKRFNDVDVAICGSPAASNSSAGSAGSATPTASSAAAAPPTVQPERKEQIEKFFKDAVRFASSSKEAKEFAIPKEDKKSKGLRLFRTPSLPQRLRFRPTPSHTDTATGSGSGASTAASTPLHSAATTPVKEAKSASRLKGKEALQYEIRHKNELIESQLSQLDVLRRHVDQLKEAEAKLREEHELATSKTDRLIEALTSENLSHKALNEQMGQEHADLLERLAAMEQQLQQQHDEHERQVEALVAESEALRLANELLQTANEDRQKVEEQLQAQLSALQADVAQAREHCSLEQAKTAENIELVENLQKTNASLLADVVQLKQQIEQDALSYGQEAKSCQAELECLKVERNTLKNDLANKCTLIRSLQDELLDKNCEIDAHCDTIRQLCREQARHTEQQQAVAKVQQQVESDLESAVEREKSYWRAELDKRQKLAENELIKIELEKQDVMVLLETTNDMLRMRDEKLQKCEEQLRNGIDYYIQLSDALQQQLVQLKQDMAKTITEKYNYQLTLTNTRATVNILMERLKKSDADVEQYRAELESVQLAKGALEQSYLVLQADAEQLRQQLTESQDALNALRSSSQTLQSEVSLKESLLHELLAGEAETLAKFNQIANSFQERIDGDAQLAHYHELRRKDETREAYMVDMKKALDEFATVLQFAQLELDNKEQMLVKVREECEQLKLENIALKSKQPGSASLLGTPGKANRSNTTDLEKIEDLLCDSELRSDCEKITTWLLNSSDKCVRQDTTSEINELLSAGKSSPRPAPRTPKAAPHTPRSPRTPHTPRTPRSAASTPKKTVLFAGKENVPSPPQKQVLKARNI q9vqr9_drome Coiled-coil domain-containing protein 47 Dmel\CG17593 CG17593-RA CG17593 Dmel_CG17593 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MRPVFAFLLLALICFASTKSESDFEEDDSDDFAEFDDGNFYAEPAAKNADSFSGGAGSSHGESRDDPPAKEPRKQAQNQESDEEDGLVEDDDEFEHFQDEEEFEGYDGGDSKDPPIDQKVEPKLNIPNIPLHFRTHWDSYWMEMLMVAGLLAYFANFFAGKAKNARLAQLWFSTHRALLDENFALVGDDGKAENENPGLIKESESLYTLWCSGRTCCEGMLVELKMIKRQDLVSLVAGLMRPQLDQAHIKIELTRGVMDAFVFAVGTKKTITKLFKEYTDLSKFCSLVGKPEDRYNVPTGFGVLSEIPEATSAILESRIITALNKYQSYIDYLHISDQFSGPIQQEEGGTLKQPETKAVLLAGFNLPKHAEMETIKPLLLLIFYLMERLKTYRMSKEGKAKSDKNRQRVEEEFLKSTHAARAEAAAQRREDKRKQEKERILADEDPEKQRRWEAKEQKRQAKRNAPKMKRLAVKSL mira-brat 361222 N/A 361224 unspecified role unspecified role prey prey N/A 361225 unspecified role unspecified role prey prey N/A 361226 unspecified role unspecified role prey prey N/A 361227 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 361228 unspecified role unspecified role prey prey association association false false false pros-brat 361223 N/A 361224 unspecified role unspecified role neutral component neutral component N/A 361225 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false