MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint wiedeman-2006-1 Essential role of Isd11 in mitochondrial iron-sulfur cluster synthesis on Isu scaffold proteins. yeast Saccharomyces cerevisiae Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom predetermined predetermined participant predetermined Wiedemann N., Urzica E., Guiard B., Müller H., Lohaus C., Meyer HE., Ryan MT., Meisinger C., Mühlenhoff U., Lill R., Pfanner N. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation wiedemann-2006-1 Essential role of Isd11 in mitochondrial iron-sulfur cluster synthesis on Isu scaffold proteins. yeast Saccharomyces cerevisiae Baker's yeast tap tandem affinity purification TAP TAP tap predetermined predetermined participant predetermined Wiedemann N., Urzica E., Guiard B., Müller H., Lohaus C., Meyer HE., Ryan MT., Meisinger C., Mühlenhoff U., Lill R., Pfanner N. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation wiedeman-2006-2 Essential role of Isd11 in mitochondrial iron-sulfur cluster synthesis on Isu scaffold proteins. in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Wiedemann N., Urzica E., Guiard B., Müller H., Lohaus C., Meyer HE., Ryan MT., Meisinger C., Mühlenhoff U., Lill R., Pfanner N. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation wiedeman-2006-3 Essential role of Isd11 in mitochondrial iron-sulfur cluster synthesis on Isu scaffold proteins. in vitro In vitro bn-page blue native page bn-page predetermined predetermined participant predetermined Wiedemann N., Urzica E., Guiard B., Müller H., Lohaus C., Meyer HE., Ryan MT., Meisinger C., Mühlenhoff U., Lill R., Pfanner N. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation isd11_yeast Protein ISD11 Iron-sulfur protein biogenesis, desulfurase-interacting protein 11 YER048W-A ISD11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPGFTAPTRRQVLSLYKEFIKNANQFNNYNFREYFLSKTRTTFRKNMNQQDPKVLMNLFKEAKNDLGVLKRQSVISQMYTFDRLVVEPLQGRKH nfs1_yeast Cysteine desulfurase, mitochondrial NFS1 SPL1 YCL017C tRNA-splicing protein SPL1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLKSTATRSITRLSQVYNVPAATYRACLVSRRFYSPPAAGVKLDDNFSLETHTDIQAAAKAQASARASASGTTPDAVVASGSTAMSHAYQENTGFGTRPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNTAVENARAHVAKMINADPKEIIFTSGATESNNMVLKGVPRFYKKTKKHIITTRTEHKCVLEAARAMMKEGFEVTFLNVDDQGLIDLKELEDAIRPDTCLVSVMAVNNEIGVIQPIKEIGAICRKNKIYFHTDAAQAYGKIHIDVNEMNIDLLSISSHKIYGPKGIGAIYVRRRPRVRLEPLLSGGGQERGLRSGTLAPPLVAGFGEAARLMKKEFDNDQAHIKRLSDKLVKGLLSAEHTTLNGSPDHRYPGCVNVSFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGRFSTEEEVDYVVKAVSDRVKFLRELSPLWEMVQEGIDLNSIKWSGH D709E326B3921BCC isu1_yeast Iron sulfur cluster assembly protein 1, mitochondrial YPL135W Iron sulfur cluster scaffold protein 1 ISU1 NUA1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLPVITRFARPALMAIRPVNAMGVLRASSITKRLYHPKVIEHYTHPRNVGSLDKKLPNVGTGLVGAPACGDVMRLQIKVNDSTGVIEDVKFKTFGCGSAIASSSYMTELVQGMTLDDAAKIKNTEIAKELSLPPVKLHCSMLAEDAIKAAIKDYKSKRNTPTMLS E240845A27D2E9EE nfs1-isd11 492527 isd11_yeast 492531 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 nfs1_yeast 492532 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6G homomint mint nfs1-isu1 492527 nfs1_yeast 492532 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 isu1_yeast 492533 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association false false false F6G homomint mint isd11-nfs1-2 492528 nfs1_yeast 492532 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 isd11_yeast 492531 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level tap tagged tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6A homomint mint nfs1-isd11-1 492529 isd11_yeast 492531 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated Stoichiometry: 1.0 nfs1_yeast 492532 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6B homomint mint isd11-nfs1-3 492530 isd11_yeast 492531 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role neutral component neutral component in vitro translated in vitro translated protein in vitro translated Stoichiometry: 1.0 nfs1_yeast 492532 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6E F6D homomint mint