IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ schimanski-2005-1 Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination. 9tryp Trypanosoma brucei affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Schimanski B., Nguyen TN., Gunzl A. In some cases the tag used was a derivative of the original TAP tag where the calcium binding peptide was replaced by the 12 amino acid epitope protein C (ProtC) for a ProC-TEV-ProtA tag. This tag is referred to as PTP in the text. An anti-ProtC matrix replaced the calmodulin affinity resin for purification of proteins. Figure 2(B). Figure 3 (B) and (D). gunzl@uchc.edu Eukaryotic Cell (1535-9778) 2005 Only protein-protein interactions imex curation q38bb7_tryb2 Tb10.70.2360 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MYRESLLGVALGATLAELESDCLLTEGQKQQLWEIFDIAMDRTLAEAPVRSQVRVRTSAPSLVGHATLGGESGLSSIDAPTLSTRPAEMEFPLPPSEVTHTVATTPCDVLDEEVLYPVYRVKDGMWTILLKDPILELTDATGSTETIRLDYLKVYLKEVVSRSATSVSASSRKKRKRSRP q387v6_tryb2 Tb10.61.0780 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MNGRYARQSGCFRGKRPREITTQKLFNRAIEHFQSIYNDSVERHERETFASRAVFEKQCGVDREHFNRAHGSLAKHGKGKDFALVLEQFENEEAKLQREHEEELQRVYPLAFLELDTAAVLSLTLIPRLKSKLDLLERQLTQRRKFTNQHQGDAAARGSHSRDVDPPVDAKESDDKNGADGVPSELNDAKTGIEEDDVGTLCVGRGAYITGDEPSEVEESDSSALVQVLDTSVEDGKVGTGTGGGFRNHVHRLVSRGLGNILSIPTDVMRAGAADFARIGLLLNTESRRQRRDERRKFLRSHMEVCGPEGKTIGKWELKRLLQEGKEDKLKQEEIFAALEAAKTMPEFTGSDMNANIVESMENDGAPRKTAGASTASRETGDTTASETVVKRHTKIVLRGNGSRRDDGVPFTRKDFEWTSLGLDDESIGEGSRMEKLAESYMWPKRTEDRIPHILCGEVIPTYFRVRQYQLDTSSPTTATVQMSATGEDGCPSTLLDDLAEATQLDPVREGMSNGEKGVVTKKSPLVFRIPFRAKSVWLKVDTLGDFIASQVKFFGAVTSREI q4krh6_9tryp protein protein 9tryp Trypanosoma brucei MQQRLVLIATDFVTLYQEALSRQLLTPAALTPDAFKDLFDRINVEYMHYAGAGATQPYFEDVVENLLQLAAAYITLPPDAAPNSRAFGVYLTFFLYATQPAIETSPVKVQISLGTLQRYVEDIDSTARDNQGVITSLGCRVSDGEKRLLLALHKSGALKVMPFIDDSLYVRTLIEVHEQAGLPLLTCVAPQRSNPSPHIALEGGTCVDDDLSNQLHAYREMRRRINTESLLKRK q57u75_tryb2 Tb927.5.3910 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MRRNNRTRCTIDDVNGMLARNAQLRNALQERYKQLKMRYEQLAALRAALSPSRGVTLRKLGVRQETADGAEEVRFLDDYTTGGVGNPPFRDAGIYSAKNIVCYAPVPPTRDELRWKGVTLAFPQLAYVHSLAMLPETANSFSKALQWSREEDSALREQVHAYKGARCGPSFWKALGAPGQSRFEVASHYIRLQQLGLIEPGKNDKTMMWIPEEQRDIALREAVWRHLGDEGGIMAAYVEIISVAARKCVYLSEVTCNESLVFPPYVWVKRTTFNWLTSLLIQKAKAPLVRCEGEFSDDTPLCMHFKSDISLKLSAEDMMACLLAFKGEVFGEVGGLRFIERAFLPKNRTYALKATDFMAKREGKKHKLDEEV q387r9_tryb2 TATA box-binding protein-like 1 TBP-like factor Tb10.61.0330 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MDNDFTGNFDMDENDFFIDEDEGDMDFLQRAAGASGVYVDAAPKVVMENNEQMKGECSDRVAEVNIQELFPNADPATVPLVVGIIAQAKLGVGVDLRALSCATRNVEFTPRVRCPAATLRLHEPNAVVQVRTSGLLGIIGSTSVGEARQATELAARIIRKALNLNFSSVQFRVRSLMARFDLCHPIRLDELAKHEGVFCSYEPDRFSGCIVRLSGSSRGNQWQVCCTVFVTGKVIVLGARSREELLDAFYTVLPIIAQYAKR q38e05_tryb2 Tb09.211.1510 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPPKQLVDPFSLQVGNKKETTLANVAAHTAVTLTDIRRRNPQLDVYKISDKLPAGTIVHLPQTLSERGNPYGCFFTIGEAEDAMRKLEARLDRLLEEEGPNLPELPTRRVLSKMERIVTQVKEEGERQLARVREEYSECERRPALLQFLVDLEPVKRLREQLDEEYDGRKLRLKVMDEGNSELFRQAEPRYPNKGIAASLRHNVIDTSEDFCLTEHSHRWEFTFFTPGSTTEPQETWAVLSCQSLGTLLDTFVCRNCIPVNISKNAFFFIGGTFYVDNRHAGEGGEDYEDLTAPIRHFDPCGEGASTEGETRQKNIAFGNCPVKYVSQTTFGDLNLRLGEYGVMRHLGWCNHYFYLSSVTSLRGFDRDDHTRAAYPQRVMKTPTRVVRCRLCRSHPATVVCYNDEISPESPCPYCVPCFELLHATDEGEVEEGKFFAIRLPQGKYYTA q4gyy5_tryb2 Tubulin alpha chain TB927.1.2340 TB927.1.2360 TB927.1.2380 TB927.1.2400 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MREAICIHIGQAGCQVGNACWELFCLEHGIQPDGAMPSDKTIGVEDDAFNTFFSETGAGKHVPRAVFLDLEPTVVDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVYHAVGGGTGSGLGALLLERLSVDYGKKSKLGYTVYPSPQVSTAVVEPYNSVLSTHSLLEHTDVAAMLDNEAIYDLTRRNLDIERPTYTNLNRLIGQVVSSLTASLRFDGALNVDLTEFQTNLVPYPRIHFVLTSYAPVISAEKAYHEQLSVSEISNAVFEPASMMTKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWSPTGFKCGINYQPPTVVPGGDLAKVQRAVCMIANSTAIAEVFARIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAESADMDGEEDVEEY q4gyy6_tryb2 Tubulin beta chain TB927.1.2330 TB927.1.2350 TB927.1.2370 TB927.1.2390 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MREIVCVQAGQCGNQIGSKFWEVISDEHGVDPTGTYQGDSDLQLERINVYFDEATGGRYVPRSVLIDLEPGTMDSVRAGPYGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELIDSVLDVCCKEAESCDCLQGFQICHSLGGGTGSGMGTLLISKLREQYPDRIMMTFSIIPSPKVSDTVVEPYNTTLSVHQLVENSDESMCIDNEALYDICFRTLKLTTPTFGDLNHLVSAVVSGVTCCLRFPGQLNSDLRKLAVNLVPFPRLHFFMMGFAPLTSRGSQQYRGLSVPELTQQMFDAKNMMQAADPRHGRYLTASALFRGRMSTKEVDEQMLNVQNKNSSYFIEWIPNNIKSSVCDIPPKGLKMAVTFIGNNTCIQEMFRRVGEQFTLMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATIEEEGEFDEEEQY q383n7_tryb2 Tb11.01.2190 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSAPMARRDYFSMGSRCCIACGQWIPSEHDMPRSGSAAAGGLQCRRCNVVQPERPMPDFEKASEVTTAALLFTKAEIDAAWKSVKHSLEEARREDVGVAQTDNRVEEEAFCEKCGVPRKCKVFARQIRSADEGQTVFYQCTSCDSEWQLNS q387i8_tryb2 DNA-directed RNA polymerase subunit beta Tb11.03.0450 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSTKNSVVGSASGSHASTAGETVTSTRSGTSSRHSRQRRQQYDYVKNPSEPMTAMERLKLVRCSIARLRYLGERHRHTSKLDTTGGDRLLSFFEGLRKRVLKLEYNPRVVAKSKVGAPKHLDYIRGHANALLGEASTIISECEQAMDRSEAVYEGLAYHQLQDTHRELENLVGLLSGAPSDMESAGSLKLLADLVSPHVAEFDAFAEQRVPEFLQELKNEGFLFSSVINDSQQPANAIGLLSVFIVDVAIGVRPGRSARLVIQHALHNLARLKSRVDKADLTLGAEEDSSNNEHHRMQSLEFEVIRGRLREAKEELERIRDKWDHDEGDIFSRAGTGVQGLQSIIDAKRVKESTVIEEAQAIHDRTVYACLPFASPQYCRIRSETYSQPCHVRVGFKFVAKGDGSVSRTKTQLIYNLMPEFLILMRQQGIAPSDQERFATLAPSKYELWLSLGELPEMVLGARCALRGVNQRLDHFRALEEQKEVGGYFVLRGGERILRNLILQRCNVPLNIERERFATVDSFFSPRAVIIRCKRPSGLTVQNYFYYATKGEVIFTFARRVVWNIPALLVLSALNTRHRTSLDIYKLLTIGLTNGRSPHAARVEALLQHHSQMPYSTLDNFLDYLAVVGLMYRRYHESSFIFRFLPQYHTSFATQHDAWYGLFMLRRHMLPHLNTAEATPDLPPTATSREIREWLSTGLQEELAAKFDALIGILRQLLVFNDGATGNQGNDVLAYQEVFTVSQVLIGAFEVSLMKTMGAVTFRMAGQLSPEIFQATMCLAETPIREAANTLEKLNQLLEYSCRNNGPDPLITLNRLLLTGNFALDREEDFYSPQTAGWAVMTEHLNFYRSFEQLMCVHRGKSIAEMRSSDVRRYPCEAYGFICMVHSPDGEDCGVLNHLSISTVLSASPRVGSSQYKILLAFIEEELKNVRNTTSLISCVDQLVGTVPVWLEGRLLGYLSPNEALSASKALTQKKALRSRGVVGVTGIVRRDSISSLNTMEVVYIPPGSKDPMGLYIFYQYGRLMRPVLQIESPSRGGDLPFPVVYLGTWEQSWRDIAAVPSDLFDGFDQLRRKYEFMEQNGFNILSFTSSTIPFFEHNCSPRNLFQCGLSKQTAGTQLQTQAWRRDAKLFRMYYPQRYISRTLPMDYFGLDDVSLGVNAVVAILAYTGYDLDDAMILNSTAKQFGMLNAGVTVAKIVKASGKGDSNDVFVFHNCLPDGQPFTPELDRHGLPKKRAVPNATAYFSFDTDHKYPSLRDSSAVYCCAKRFEYIDPLTNEKTYEYTRHQITKWRHFDKGEDAWVHSVIPLTYDGPDPTSVLVLFRIPRPPVIGDKFSSRHGQKGTLPLDIRSHDLPFSTRDGITPDIIINPHAFPSRMTVGMVLEMMGAKLGCIQGRLCDHSAWSVIDEQPRSAKTIGDALEALGYNRYGREKLIDGITGEEIEAEVLVGISGYQRLRHMVSDKWQARARTDSHTHRAVTKTGQPVKGRKRHGGVRVGEMERDALLSHGVAEVVIDRLLHVSDKTKAFICVECGSMLSIYERHATEYSTWKTCKFCSAGLHETDDTIAFVEIPQVLRLWATELTSVGIRVVLKTSETSL q387x6_tryb2 Plastid-encoded RNA polymerase subunit alpha Tb10.61.1040 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVKVYNLQRRTETLPTVRRTAPNAEELEYQSHLSRPAGAGDGEPTRRVETITFDMDNVSPPVANMFRRLMLTEVPVFAFDRILIEHNDGVVPDELLAHRIGLVPLAGPVSSMQYITDSASVGFDNLDPERVLLFELDVTAKRDVPTTSVYSGHLKWQPIAKQEELAARSEDDRVFLVHPDIVLTRLGPGQRLKLRAIAIKGIGAVHAKWTPVAACFYEMKQAKAGAEDSDGRASGRGRSRSGRGRVDANEEESNATRPRSASGNHSHEPHRETVNEVIAKRDTSSVRFTVESIGQLYAVDVFRQALILFAERIRDLAVRIRSTEPRMTGAAATAFTE q388r6_tryb2 Tb10.389.0120 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MDSVKSFKMIRLYRAFNTVIQMAIDRGFVVSHPSDVANAIRDSRLYDITDGLNYQWFTQRCSSYLQRHRQTTAEEEEEAKQEGRPGKDPSKSKRTKALRDASQDFLYSSLELVCKGSSSRIGFGGGAISCKTEDEVDGRGPTAEPSSRKKNETILCAFFVQSLGVDVLNNLREVAQRRNASSMIVVVGSKVRGGRRSIREAGGGLHPGTTGQVIRIQVFEEDELAYNISRHQSVPKHVPMTDVEVRAFLEQRKLMITQLPRILNDDPMVEYLDLPRGSIIRIERRTDEGSTYEMYRHVI q57ul1_tryb2 Tb927.8.5390 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSTVNRLQCIGKLGEGTYGVVYKARELATGQIVAYKRIIVGSEDDGAPSTAIREIALLKVLRHNNIVRLYDVLFEPPKLTLIFEYCEYDLRRFMHKHDSRVRTQVKEILKQVLLGLRYMHQRSVVHRDLKPDNIFVRVDGISGSEGSEMSVSSRETEVVNTRGHWDDIDEAANCPKNANPTENPNARIASEDHGADGTVPSGSTTPTSQLVIKLGDYGMARIESIPVKKYSHDVASLWYRSPDALLGSAMYGFAVDLWSVGCIFAEMVTGAPLIRGNTDVDQLLKTFKLLGTPTPETWPSMKNCPKAVQLLKAAAELARGEPAENGKKTKNSRGVHKQHERNLPNVQKTATGTSHLSAVMRVGIDGESGCVADPRDELYHFPPELCFPSAFDEYVKASGFQGRVGVEGTDFLRRLIRYEPSQRMSVQEALSHPFVSNTVALMQRPLDVMASMLRQSLREHSVYP snap50 PTP tagging reveals SNAP50 interacting proteins. 357389 N/A 357390 unspecified role unspecified role prey prey N/A 357391 unspecified role unspecified role prey prey N/A 357392 unspecified role unspecified role prey prey N/A 357393 unspecified role unspecified role prey prey N/A 357394 unspecified role unspecified role prey prey N/A 357395 unspecified role unspecified role bait bait n terminal ProtC-TEV-ProtA tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false snap50-1 PTP tagging reveals SNAP50 interacting proteins. 357389 N/A 357394 unspecified role unspecified role prey prey N/A 357390 unspecified role unspecified role prey prey N/A 357391 unspecified role unspecified role prey prey N/A 357392 unspecified role unspecified role prey prey N/A 357393 unspecified role unspecified role prey prey N/A 357395 unspecified role unspecified role bait bait c terminal ProtC-TEV-ProtA tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false snap50-2 PTP tagging reveals SNAP50 interacting proteins. 357389 N/A 357391 unspecified role unspecified role prey prey N/A 357392 unspecified role unspecified role prey prey N/A 357395 unspecified role unspecified role bait bait c terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false rpa1 PTP tagging reveals RPA1 interacting proteins. 357389 N/A 357396 unspecified role unspecified role prey prey N/A 357397 unspecified role unspecified role prey prey N/A 357398 unspecified role unspecified role prey prey N/A 357399 unspecified role unspecified role prey prey N/A 357400 unspecified role unspecified role prey prey N/A 357401 unspecified role unspecified role prey prey N/A 357402 unspecified role unspecified role bait bait c terminal ProtC-TEV-ProtA tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false tfall-1 PTP tagging reveals TFAII-1 interacting proteins. 357389 N/A 357390 unspecified role unspecified role prey prey N/A 357391 unspecified role unspecified role bait bait c terminal ProtC-TEV-ProtA tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 357392 unspecified role unspecified role prey prey N/A 357393 unspecified role unspecified role prey prey N/A 357394 unspecified role unspecified role prey prey N/A 357395 unspecified role unspecified role prey prey association association false false false