UniProt UniProt http://www.uniprot.org afshar-2005-2 Cortical localization of the Galpha protein GPA-16 requires RIC-8 function during C. elegans asymmetric cell division. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Afshar K., Willard FS., Colombo K., Siderovski DP., Gonczy P. The proteins gpr-1 and/or gpr-2 may also interact with goa-1 and gpa-16; due to the high degree of sequence similarity, these two proteins cannot be distinguished in coimmunoprecipitation experiments. pierre.gonczy@isrec.unil.ch Development (0950-1991) 2005 afshar-2005-1 Cortical localization of the Galpha protein GPA-16 requires RIC-8 function during C. elegans asymmetric cell division. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Afshar K., Willard FS., Colombo K., Siderovski DP., Gonczy P. The proteins gpr-1 and/or gpr-2 may also interact with goa-1 and gpa-16; due to the high degree of sequence similarity, these two proteins cannot be distinguished in coimmunoprecipitation experiments. pierre.gonczy@isrec.unil.ch Development (0950-1991) 2005 afshar-2005-3 Cortical localization of the Galpha protein GPA-16 requires RIC-8 function during C. elegans asymmetric cell division. in vitro In vitro biochemical biochemical predetermined predetermined participant predetermined Afshar K., Willard FS., Colombo K., Siderovski DP., Gonczy P. The proteins gpr-1 and/or gpr-2 may also interact with goa-1 and gpa-16; due to the high degree of sequence similarity, these two proteins cannot be distinguished in coimmunoprecipitation experiments. Time-course of [35S]GTP{gamma}S binding to 100 nM GPA-16 in the presence or absence of 10 �M GPR-1/2 peptide studied. pierre.gonczy@isrec.unil.ch Development (0950-1991) 2005 gpa16_caeel Guanine nucleotide-binding protein alpha-16 subunit gpa-16 spn-1 Y95B8A.5 protein protein caeel Caenorhabditis elegans MGCIMSQEDEAAKRRSKKIDRLLKEDGENSMRTIKLLLLGAGESGKSTILKQMRIIHDVGYTTEERKVFRGVVYGNIILSLNAIIHAMEQLKISFTTLDHESDARKLLMFSTTGEEDELPEELVVLMKSVWSDSGIQKALERSREYQLNDSAGYYLSQLDRICAPNYIPTQDDILRTRIKTTGIVETQFVYKDRLFLVFDVGGQRSERKKWIHCFEDVTALIFCVALSEYDMVLVEDCQTNRMRESLKLFDSICNNKWFVETSIILFLNKKDLFEEKIVRSPLTHCFPEYTGANNYEEASAYIQQQFEDMNKRTTGEKNQEIYTQFTCATDTNNIRFVFDAVTDIIIRDNLRTCGLY gpr1_caeel G-protein regulator 1 F22B7.13 gpr-1 protein protein caeel Caenorhabditis elegans MDVSYYDGPKDEVAEAMLKSAVTAMRLGQYEDGKGRLEEIMEFGTSNFQLLGTIYMYYGRVCRHLNHDAKALEFFEHELNMFKLIFNYPEACDSTRRIVEQALKMGKFPKARRFAEDLIDYTSNKKNGEKYIGQARILFASVCLEGCERDVESNQDEKKKLLSICAEQIAAVKLFNENNTEGAVSETKIMLLEAKCLSLDEKYEESRRKYQECIDFAIKTDQFEAVHIAYYDKALYAETDLLFFIIRDLRSALFYATKFGKERDVVKYKSKLSEEMLRNGEFHEAYLYGLEALVSIRKLGLNEYIGDVLLTIAKCLIALGKRRQAAYFIILGSVLTINQNSFKLFYEQIDVAMNQERSETATDQDVCLAIDSSPDPTSSNDMINKFVVELEHATNVETWEMIVNGIIDDQKKPVAIEKKENEEPVDMMDLIFSMSSRMDDQRTELPAARFIPPRPVSSASKKTTKSHRILPGLRANWTKVQSMKFDGHTMNRILKRSKKSKSSLDSTNSMQGDDTRSDDVTMTSK q9gsx9-1 Synembryn ric-8 Resistance to inhibitors of cholinesterase protein 8 Y69A2AR.2 protein protein caeel Caenorhabditis elegans MSEELHSDLIASIFGGKPAKIEEFFSKWNFANAAVSKFDMANSAKNELGDRICEVIENGELTHVLLETIKILSREKDGLEGLLNDPLCDKILAFAELSSNENNSKTVHTLMEAQKCLINTLFHSQRMRDRFYANPKTGENLQFFLGEFEENRRKTSSIDWIRLLNPVQAAEIWYFYHRIAFIATALGREFQKNWANDPKTIDSLLLAVEICTNRSENSTQDINRATEALKTFFNVFCHFHGDVKAIDHKNAAKTCQILRDAICSDVLTDDVVQSAIHCLSVPPLPMVLSVLCGKNSKNNGGENEEEKFFVEELSNMQLTEAILMHLDKQLTKVVALLLNDAPNQQQNPMLSAEASTLTDLVGPYFQVLARLCTDSKYVRRYCRIRVIPPLVSEEVQKRPEENNTLRGRIARIMMLPSSTKDVAAEFLFIICKRSVNRMIKYLGFGHSAGHLANLGLLGQINQPKHASDSEDSETEDYNQIKDSVNPVTGAIYPSDHGSALAGMSEEQKEYEAMKLVDAMNQMMETGIVKPGTIGDDGKIREVSHVLELLKNAPEPAPAENSDSDEE gnao_caeel Guanine nucleotide-binding protein G(o) subunit alpha goa-1 C26C6.2 protein protein caeel Caenorhabditis elegans MGCTMSQEERAALERSRMIEKNLKEDGMQAAKDIKLLLLGAGESGKSTIVKQMKIIHESGFTAEDYKQYKPVVYSNTVQSLVAILRAMSNLGVSFGSADREVDAKLVMDVVARMEDTEPFSEELLSSMKRLWGDAGVQDCFSRSNEYQLNDSAKYFLDDLERLGEAIYQPTEQDILRTRVKTTGIVEVHFTFKNLNFKLFDVGGQRSERKKWIHCFEDVTAIIFCVAMSEYDQVLHEDETTNRMHESLKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICFPEYSGRQDYHEASAYIQAQFEAKNKSANKEIYCHMTCATDTTNIQFVFDAVTDVIIANNLRGCGLY gpr-1_gpa-16_1 509120 N/A 509123 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 509124 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false This interaction is stimulated by GDP binding to gpa-16. Fig. 5A ric-8_gpa-16_1 509120 N/A 509125 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 509123 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false This interaction is stimulated by GDP binding to gpa-16. Fig. 5B ric-8_goa-1_6 509120 N/A 509125 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 509126 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false This interaction is stimulated by GDP binding to goa-1. Fig. 5E gpa-16_ric-8_1 509121 N/A 509123 unspecified role unspecified role bait bait N/A 509125 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2A, 5G goa-1_ric-8_2 509121 N/A 509125 unspecified role unspecified role prey prey N/A 509126 unspecified role unspecified role bait bait physical association physical association false false false This interaction is inhibited by GTP-gamma S. Fig. 5H gpr-1_gpa-16_2 509122 N/A 509123 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 509124 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5C goa-1_ric-8_3 509122 N/A 509125 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 509126 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5F