MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint itoh-2005-1 Reorganization of actin cytoskeleton by FRIED, a Frizzled-8 associated protein tyrosine phosphatase. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Itoh K., Lisovsky M., Hikasa H., Sokol SY. Dev. Dyn. (1058-8388) 2005 itoh-2005-2 Reorganization of actin cytoskeleton by FRIED, a Frizzled-8 associated protein tyrosine phosphatase. xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. pull down pull down predetermined predetermined participant predetermined Itoh K., Lisovsky M., Hikasa H., Sokol SY. Dev. Dyn. (1058-8388) 2005 fzd8_xenla Frizzled-8 fzd8 fz8 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MESLSLSLLLLVSWLQGSQCAAAKELSCQEITVPLCKDIGYNYTYMPNQFNHDTQDEAGMEVHQFWPLVVIHCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCERARAGCAPLMRQYGFAWPDRMRCDRLPEQGNPDTLCMDYYNRTEQTTAAPSHPEPPKPPARSVPKGRTRVEPPRSRSRATGCESGCQCRAPMVQVSNERHPLYNRVRTGQIPNCAMPCHNPFFSPEERTFTEFWIGLWSVLCFASTFATVSTFLIDMERFKYPERPIIFLSACYLLVSTGYLIRLIAGHEKVACSRGELDLEHIIHYETTGPALCTLVFLLIYFFGMASSIWWVILSLTWFLAAGMKWGNEAIAGYSQYFHLAAWLVPSIKSIAVLALSSVDGDPVAGICFVGNQNLDNLRGFVLAPLVIYLFIGSMFLLAGFVSLFRIRSVIKQGGTKTDKLEKLMIRIGIFSVLYTVPATIVVACFFYEQHNRQGWEVAHNCNSCQPEMAQPHRPDYAVFMLKYFMCLVVGITSGVWIWSGKTLESWRAFCTRCCWGSKATGGSMYSDVSTGLTWRSGTGSSVSCPKQMPLSQV mint Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activation by Wnt8, Wnt5A or Wnt3A induces expression of beta-catenin target genes. Displays an axis-inducing activity q6ehh9_xenla FRIED protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MHVSLAEALEVRGGPLEEEELWAVLNQSAESLQELLRRADPAALGFIISPWSLLLLPSGSVSFTDENISNQDLRVFTAPEVLQTKSLSSLSAIEKMHIYSLGMTLYWGADYEVPETQPIQLGERLNSILLDMCDDQFYTRLSSRNSLGWLQCPYQKQQLCPSYYYIKQLVKLVLGSLSRVDQLGLKTEEPDRSQAIRDRLRGKGLPTGRTSTVDSFDAQTAISQQAFFNRGLSKSMGFLAFKDQSEEEDSCHFTSSDYNSGYENEVNSHHSHHQLMPSELDRKNYDSGQRKKNWVSSFDLLSLKDQSTEKDFSTNHHRPPRYPXTETIQISNLPKIKDRQLDGSSSTDVLGPFRLDQARHGLEVPTAVAISSAWTGSRKETKIQVLRAAMTLEEPARLHRTSHSDACSSSSESQSIVSSDPEFRQGLKRKDSTSVVAGLEDTDRLQLPGLRSNQHYENPPDGANIRQEMRKRQEEDMIEMQARMAIRQSRLNLYSGDVPRTSMLDLNRDPLREIAMENSLAQRNLRSFLGPEFVKMTTEPCVTLDLPPSILAKKGKTDEIRRKLNIILLTGQRIELICDTKSTCKDVFDIVVAHVGLVEHHLFGFAILKDQEFYFIDLELKLSKVSPDGWKEDTKKKGKSAINFNLYFRVKFFVDDISFLQHSLTCHQYYLQQRKDILDGKLYCDDEAAMLLASLALQAEYGDYQPEVHSMSYFRMEHFLPARVTEKLELTYLKEELPRLHSTYAGASVKEAELEFLRVCQKLPEYGVHFHRVQPEKKSQTGILLGVCPKGVLIFEVHNGTRTPVLRFPWRETKKVSFSKKKISLQNTSDSIKHVFQTDSNKTCQYLLQLCSSQHKFQLQMRARQSSQETKEIEITSMRSLNLQAESVRGFNMGRAISTSSLASSTLNKTAVRPMSIQPDLLKRLSCSDLSALQTHLSNGTRDRKTKNPWEERPRIISKSYHDLSQVAYSPQQMMLNTRMDNPPYSIEGLLSQASLNQMAMSDAESVTGNGRLNKSFSSVHRSLDLQKPESDSSSMDDSGHAYVVGVSMHSVANTSSPVVFKGNKNPNNCVTPTSTSDREITLVKLKKDPKYDFGFQIVGGDTCGKVELGIFISSITPGRPADLDGRLKPGDRLISINSVSLEGVSHQSALDILQGCPEDVSILVSQPKEKFLKDNQSTHSSSHSQRVFPLQDIEADSSSEEQSKLRGHQRLISGSSFGMSVGKCEGSLSSQDSRTESASLSNSQMKSPNNSAKEHPAKQSARTAKYPSLDSTKAGDRKRTQGDGGKGKRSGLTESVDSDRGDSDMDEETIQVARSKGQPRRNPLLRGQHRNELHKASPTPLKPGDAFFVVLEKNDDSLGISVTGGVNTSVKHGGIYVKAVIPKGAAEADGRIQKGDRVLSVNGTSLEGATHKQAVEMLRNTGQVVTLQLEKGQLPVTKVHAPVTPQGTLPIQHDQVVLQGMDRKKTLDYSFVTDENLFEVKLIKNSSGLGFSFSREENNFPGQAGSSIVRVKKLFPGQPASESGKIDIGDVIFKVNGVALKGLSQQEVVSVLRGTSSEVTLLMCRPPAGVLPEISPSMLTPLKSPLPSKSNDGQCNTKGTGNSSDEDEEANEKSMRRLKNPSRRDSYSDSSGTGEEDIISVTDEIATWNATIYQTPSSAIAPNHSQFNTPGIREDTIHTNFYTPQESPCKPELEDSSNPPSPMPYDLTPNQSAVIPQIVLPDAFLSESFSEEQLSIKSLGLKDLEEFEPEVELHVTLTKSEKGSLGFTVTKASDCIGCYIHDIIQDPAKSDGRLRPGDRLIKVNDVDVANMSHTEAVNLLRAAPKTVRLVLGRVLEFPRPQFPSHLLPDIVLTCHKGNIGLQLSSGPDSVSQNIYIQNIKAGSVADCDGSLKPQDILHYINGESTVGMTIEEAEQALESSLPTVALKATRDGTPVVPTSRKTVSQSPKTTKMNGFSSDEGIICIQAAPDVFTPEAGIVQILLEKPASGELGFSLIGGEYGIFVKSISPGGVADTEGSLQVGDRLLQVNGENMIGATHGKAVASIRKTKGTLQISVAREAMPSDTTILNCNGNIDSMTHIEDNRYNEDEDNDVIQSLLDVVHEQTQNFVELNLQNALLSEHFSVEQIEDGLEEKAEDTDCDGSSLPDDSPEQTKVNGVGYYRNKERTQRSAVDDDITWPSTGGQYKLFYKNFSVTKEDLSSLSLVKVQPSSRYTGFSLKAVIQSIHGKLEENTPSREFELLQDLKPTDGCLIGQTKENKKKNRYKNILPYDATRVSLGAENDYINASFIKMPVGNEEFVYIACQGPLPSTVSDFWQMVWEQNSSVISMMTQEIEGGKIKCQRYWPEEPGRPLMISNQLQLTLVMTQHLESFVLRVLELHDIQSEEVRQIAHLNYTAWPDHDTPSDPYQLLTFISFMRHIYKSGPIITHCSAGIGRSGTLICIDVMLALISKDLEFDISNMVHTMRLQRHGMIQTEEQYIFCYQVILYILKRLLAEEKTVK fragmentation type: fragment mint fzd8-fried 367006 fzd8_xenla 367008 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q6ehh9_xenla 367009 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false NFA mint fzd8-fried-1 367007 q6ehh9_xenla 367009 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tagged myc tagged myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 fzd8_xenla 367008 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst tagged gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F2B mint