IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhao-2005a-1 Amino acid residues associated with enzymatic activities of the isomerizing phycoviolobilin-lyase PecE/F. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Zhao KH., Wu D., Zhou M., Zhang L., Bohm S., Bubenzer C., Scheer H. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Detection of Lyase activity. Chromophore reconstitution with the Mastigocladus laminosus apoprotein pecA was assayed. Table 1, Table 2 khzhao@163.com,hugo.scheer@lmu.de Biochemistry (0006-2960) 2005 Only protein-protein interactions imex curation khzhao@163.com zhao-2005a-2 Amino acid residues associated with enzymatic activities of the isomerizing phycoviolobilin-lyase PecE/F. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Zhao KH., Wu D., Zhou M., Zhang L., Bohm S., Bubenzer C., Scheer H. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Detection of Lyase activity. Chromophore reconstitution with the Mastigocladus laminosus apoprotein pecA was assayed. Table 1, Table 2 khzhao@163.com,hugo.scheer@lmu.de Biochemistry (0006-2960) 2005 Only protein-protein interactions imex curation khzhao@163.com pecf_masla Bilin biosynthesis protein PecF pecF protein protein masla Mastigocladus laminosus Fischerella sp. MIAARFTNSKQLISQLNCALSPADALCAIAAISDSETTEVAVISALLQLLSRHHHHSSVATAAVEVLVKLAPASVEPLLAAFRSCSDQGFQAWIIQALAMIGDAKAFDLLAEVVGTEVANHCQGNVRRIAARGLGKIGSTVKDREVTDRAIEKLHWALVTPQDWGLRYAAVVSLQEIATPQAHAVLSAAVAGESDWVVRSRMKKALEQIPAC pece_masla Bilin biosynthesis protein PecE pecE protein protein masla Mastigocladus laminosus Fischerella sp. MTAACCAQPILSPEAAIAALAGEDNQIRYYAAWWLGKHQVQAGCAALCEALFDERYRIPSGGYPLRRQAARALGQLKNPQAVPALIAALACEEDLGLREAVIQALAMIGDRRAVHPLVQLLQSQQPQPYEALIEALATLQVWSARPQIEPFLYHSSERVQCAAARYLYLLTKQPQYLERIVQNLNHDNMYLRWAAIFDLAALGHRQAVDAILAAKVPNSLKLLNLKRILETLLDGDRSQLNNGEFCQNQSDRETIELLFQAIDDLLIQL pece-pecf-1 Formation of a PecE/F complex with a chromophore lyase activity. 366646 N/A 366648 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 366649 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false Proteins alone does not have chromophore lyase activity on pecA. P29729, P29730. purified. When the following region of PecE, 29-34 (YAAWWL), 263-265 (DLL) or 111-122 are deleted, the complex lost its activity. Histidine 121 and Cysteine 122 of PecF are essential for the enzymatic activity. The Km is 10E-6, vmax is 0.15E-9 M s-1. The kcat is 2.1E-5 s-1.The catalytic efficiency kcat/Km is then 2.1E-6 M s-1. The catalytic efficiency decreased 16-fold when the unique four histidine residues in PecF (53-56). pece-pecf-2 Complex formation with His-PecF and PecE shown by pull-down 366647 N/A 366648 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 366649 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false P29729, P29730. purified.