IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sawa-2005-1 DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Sawa S., Koizumi K., Naramoto S., Demura T., Ueda T., Nakano A., Fukuda H. sawa@biol.s.u-tokyo.ac.jp Arabidopsis cDNA library Plant Physiol. (0032-0889) 2005 Only protein-protein interactions imex curation sawa-2005-2 DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sawa S., Koizumi K., Naramoto S., Demura T., Ueda T., Nakano A., Fukuda H. sawa@biol.s.u-tokyo.ac.jp Arabidopsis cDNA library Plant Physiol. (0032-0889) 2005 Only protein-protein interactions imex curation sawa-2005-3 DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Sawa S., Koizumi K., Naramoto S., Demura T., Ueda T., Nakano A., Fukuda H. sawa@biol.s.u-tokyo.ac.jp Arabidopsis cDNA library Plant Physiol. (0032-0889) 2005 Only protein-protein interactions imex curation sawa-2005-4 DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis. arath-leaf Arabidopsis thaliana leaf leaf leaf confocal microscopy confocal microscopy predetermined predetermined participant predetermined Sawa S., Koizumi K., Naramoto S., Demura T., Ueda T., Nakano A., Fukuda H. sawa@biol.s.u-tokyo.ac.jp Arabidopsis cDNA library Plant Physiol. (0032-0889) 2005 Only protein-protein interactions imex curation agd3_arath ADP-ribosylation factor GTPase-activating protein AGD3 SFC Protein SCARFACE AGD3 T31B5.120 At5g13300 VAN3 Protein ARF-GAP DOMAIN 3 Protein VASCULAR NETWORK 3 FKD2 Protein FORKED 2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MHFTKLDDSPMFRKQLQSMEESAEILRERSLKFYKGCRKYTEGLGEAYDGDIAFASALETFGGGHNDPISVAFGGPVMTKFTIALREIGTYKEVLRSQVEHILNDRLLQFANMDLHEVKEARKRFDKASLTYDQAREKFLSLRKGTKSDVAAALEQELHTSRSMFEQARFNLVTALSNVEAKKRFEFLEAVSGTMDAHLRYFKQGYELLHQMEPYINQVLTYAQQSRERSNYEQAALNEKMQEYKRQVDRESRWGSNGSNGSPNGDGIQAIGRSSHKMIDAVMQSAARGKVQTIRQGYLSKRSSNLRGDWKRRFFVLDSRGMLYYYRKQCSKPSGSGSQLSGQRNSSELGSGLLSRWLSSNNHGHGGVHDEKSVARHTVNLLTSTIKVDADQSDLRFCFRIISPTKNYTLQAESALDQMDWIEKITGVIASLLSSQVPEQRLPGSPMGSGHHRSASESSSYESSEYDHPTTEEFVCERSFLGYNERPSRSFQPQRSIRKGEKPIDALRKVCGNDKCADCGAPEPDWASLNLGVLVCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISLFQALGNTFANTVWEELLHSRSAIHFDPGLTVSDKSRVMVTGKPSYADMISIKEKYIQAKYAEKLFVRRSRDSDFPQSAAQQMWDAVSGNDKKAVYRLIVNGDADVNYVYDQTSSSSLTLSRVILVPERPKREDVLLRLRNELLDRTGSSSNISPEGSGGSSLLHCACEKADLGMVELLLQYGANVNASDSSGQTPLHCCLLRGKVTIARLLLTRGADPEAMNREGKTALDIAAESNFTDPEVLALLSDTNGYNHRQC drp1a_arath Phragmoplastin DRP1A DRP1A ADL1 ADL1A AG68 Dynamin-like protein 1 Protein RADIAL SWELLING 9 RSW9 MJC20.19 At5g42080 Dynamin-like protein 1A Dynamin-related protein 1A protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MENLISLVNKIQRACTALGDHGDSSALPTLWDSLPAIAVVGGQSSGKSSVLESIVGKDFLPRGSGIVTRRPLVLQLQKIDDGTREYAEFLHLPRKKFTDFAAVRKEIQDETDRETGRSKAISSVPIHLSIYSPNVVNLTLIDLPGLTKVAVDGQSDSIVKDIENMVRSYIEKPNCIILAISPANQDLATSDAIKISREVDPSGDRTFGVLTKIDLMDKGTDAVEILEGRSFKLKYPWVGVVNRSQADINKNVDMIAARKREREYFSNTTEYRHLANKMGSEHLAKMLSKHLERVIKSRIPGIQSLINKTVLELETELSRLGKPIAADAGGKLYSIMEICRLFDQIFKEHLDGVRAGGEKVYNVFDNQLPAALKRLQFDKQLAMDNIRKLVTEADGYQPHLIAPEQGYRRLIESSIVSIRGPAEASVDTVHAILKDLVHKSVNETVELKQYPALRVEVTNAAIESLDKMREGSKKATLQLVDMECSYLTVDFFRKLPQDVEKGGNPTHSIFDRYNDSYLRRIGSNVLSYVNMVCAGLRNSIPKSIVYCQVREAKRSLLDHFFAELGTMDMKRLSSLLNEDPAIMERRSAISKRLELYRAAQSEIDAVAWSK van3-drp1a Yeast two hybrid assay reveals interaction between VAN3 protein and DRP1A protein 506435 N/A 506439 unspecified role unspecified role bait bait N/A 506440 unspecified role unspecified role prey prey physical association physical association false false false drp1a-van3 Directed yeast two hybrid assay reveals interaction between VAN3 protein and DRP1A protein 506436 N/A 506439 unspecified role unspecified role prey prey N/A 506440 unspecified role unspecified role bait bait physical association physical association false false false Figure 1A van3-drp1a-1 Pulldown assay reveals interaction between VAN3 protein and DRP1A protein 506437 N/A 506439 unspecified role unspecified role prey prey region GAL4 DNA binding domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 506440 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Figure 1B van3-drp1a-2 Colocalisation of VAN3 protein and DRP1A protein 506438 N/A 506440 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 506439 unspecified role unspecified role neutral component neutral component region modified yellow fluorescent protein (VENUS) yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Figure 4