UniProt UniProt http://www.uniprot.org wei-2005-1 Molecular organization of the Ndc80 complex, an essential kinetochore component. trini Trichoplusia ni (Cabbage looper) Cabbage looper chromatography chromatography technology column chromatography chromatography predetermined predetermined participant predetermined Wei RR., Sorger PK., Harrison SC. schadmin@crystal.harvard.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2005 Only protein-protein interactions imex curation wei-2005-2 Molecular organization of the Ndc80 complex, an essential kinetochore component. in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Wei RR., Sorger PK., Harrison SC. schadmin@crystal.harvard.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2005 Only protein-protein interactions imex curation wei-2005-3 Molecular organization of the Ndc80 complex, an essential kinetochore component. in vitro In vitro chromatography chromatography technology column chromatography chromatography protein sequence protein sequence identification protein sequence Wei RR., Sorger PK., Harrison SC. schadmin@crystal.harvard.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2005 Only protein-protein interactions imex curation spc24_yeast Kinetochore protein SPC24 SPC24 YM9718.16C YMR117C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQKDNLLDNPVEFLKEVRESFDIQQDVDAMKRIRHDLDVIKEESEARISKEHSKVSESNKKLNAERINVAKLEGDLEYTNEESNEFGSKDELVKLLKDLDGLERNIVSLRSELDEKMKLYLKDSEIISTPNGSKIKAKVIEPELEEQSAVTPEANENILKLKLYRSLGVILDLENDQVLINRKNDGNIDILPLDNNLSDFYKTKYIWERLGK 8BE4B8619DD4C8FB spc25_yeast Kinetochore protein SPC25 YER018C SPC25 protein protein yeast Saccharomyces cerevisiae Baker's yeast MASIDAFSDLERRMDGFQKDVAQVLARQQNHARQQLQQFQAEMRQLHNQHQHLIDELQRLATQRTALQQQIHAAQQATNTTREQWRSYHERESELSRRQSTLAAQSRELDSLLQQRGKECVQLRARWAAQSGNDAAEVALYERLLQLRVLPGASDVHDVRFVFGDDSRCWIEVAMHGDHVIGNSHPALDPKSRATLEHVLTVQGDLAAFLVVARDMLLASL 1BED6CCFF330A5D8 ndc80_yeast Kinetochore protein NDC80 NDC80 80 kDa spindle component protein HEC1 Nuclear division cycle protein 80 TID3 Two-hybrid interaction with DMC1 protein 3 YIL144W protein protein yeast Saccharomyces cerevisiae Baker's yeast MQSSTSTDQHVLHHMDPHRFTSQIPTATSSQLRRRNSTNQGLTDMINKSIARNTISGTGIPTGGINKNKRTRSTVAGGTNGTALALNDKSNSRNSVSRLSINQLGSLQQHLSNRDPRPLRDKNFQSAIQEEIYDYLKKNKFDIETNHPISIKFLKQPTQKGFIIIFKWLYLRLDPGYGFTKSIENEIYQILKNLRYPFLESINKSQISAVGGSNWHKFLGMLHWMVRTNIKLDMCLNKVDRSLINQNTQEITILSQPLKTLDEQDQRQERYELMVEKLLIDYFTESYKSFLKLEDNYEPSMQELKLGFEKFVHIINTDIANLQTQNDNLYEKYQEVMKISQKIKTTREKWKALKSDSNKYENYVNAMKQKSQEWPGKLEKMKSECELKEEEIKALQSNISELHKILRKKGISTEQFELQNQEREKLTRELDKINIQSDKLTSSIKSRKLEAEGIFKSLLDTLRQYDSSIQNLTRSRSQLGHNVNDSSLKINISENLLDRDFHEGISYEQLFPKGSGINESIKKSILKLNDEIQERIKTIEKDNITLEKDIKNLKHDINEKTQINEKLELELSEANSKFELSKQENERLLVAQRIEIEKMEKKINDSNLLMKTKISDAEELVTSTELKLEELKVDLNRKRYKLHQQVIHVIDITSKFKINIQSSLENSENELGNVIEELRNLEFETEHNVTN 7DBC492227A80093 nuf2_yeast Kinetochore protein NUF2 YOL069W NUF2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRNQDVFPILDLQELVICLQSCDFALATQENISRPTSDYMVTLYKQIIENFMGISVESLLNSSNQETGDGHLQEENENIYLDTLNVLVLNKICFKFFENIGVQDFNMTDLYKPEAQRTQRLLSAVVNYARFREERMFDCNSFILQMESLLGQLRSKFDDYNLIQQQLKQYEDVDGDNIPDEQELQKLEEQNKELEIQLKKLTKIQETLSIDYNDYKISKQSIFKDLEALSFQIVELESNRDKLIKISNTDMEELSEGIKELNDLLIQRKKTLDDLTAQQKNLQDTVTTFETIISELYDVLRIISSEVQESNRTETELVGLKQNLINNKLKLMNVLETGIMYKLEILQEQLDLQLKNLEKLSQDTKEESRLNDTKLMDLQIKYENEIKPKIDKTDIFIQEELISGKINKLNDEIKQLQKDFEVEVKEIEIEYSLLSGHINKYMNEMLEYMQ ndc80_nuf2_1 pull down and size exclusion chromatography 507954 N/A 507957 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507958 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507959 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507960 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false Tid3 complex Fig. 1A ndc80_nuf2_2 pull down and size exclusion chromatography 507954 N/A 507959 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507957 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507958 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507960 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false Tid3 complex. Fig. 1B, 1C ndc80_nuf2_3 pull down and size exclusion chromatography 507954 N/A 507959 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507960 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false Fig. 1B, 1C spc24_spc25_1 pull down and size exclusion chromatography 507954 N/A 507957 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507958 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false Fig. 1B, 1C ndc80_nuf2_4 rotary shadowing electron microscopy of the ndc80 complex 507955 N/A 507958 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507957 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507959 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 507960 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false Rod like complex around 570 angstroms in length with globular domains at each end. Tid3 complex. Fig. 2 ndc80_nuf2_5 507956 N/A 507960 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 507957 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 507958 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 507959 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Tid3 complex. Fig. 3B