UniProt UniProt http://www.uniprot.org kim-2005a-1 Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim Y., Chattopadhyay S., Locke S., Pearce DA. david_pearce@urmc.rochester.edu Eukaryotic Cell (1535-9778) 2005 Only protein-protein interactions imex curation kim-2005a-2 Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kim Y., Chattopadhyay S., Locke S., Pearce DA. david_pearce@urmc.rochester.edu Eukaryotic Cell (1535-9778) 2005 Only protein-protein interactions imex curation btn2_yeast Protein BTN2 YGR142W BTN2 Batten disease protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSIFNSPCVFEQLPSFSQPLHSRYFDCSSPVSYYPECKRRKAIKANLRAPKKSDANCSEPLRYALAETPNGYTLSLSKRIPYELFSKYVNEKLGELKENHYRPTYHVVQDFFGNQYYVEDEADEDALLRSALKDLDFRAIGKKIAKDLFQDYEIELNHRGDELSILSKKDKIFKEFSLDQVFEDVFVIGCGVENIDDGSREKYALLKIGLVKHEEEISEGGINEPKMPIIESKIDESHDDVNMSESLKEEEAEKAKEPLTKEDQIKKWIEEERLMQEESRKSEQEKAAKEDEERQKKEKEARLKARKESLINKQKTKRSQQKKLQNSKSLPISEIEASNKNNNSNSGSAESDNESINSDSDTTLDFSVSGNTLKKHASPLLEDVEDEEVDRYNESLSRSPKGNSIIEEI E7AE1EB1E3612F3E ist2_yeast Increased sodium tolerance protein 2 YBR0809 YBR086C IST2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQTITSLDPNCVIVFNKTSSANEKSLNVEFKRLNIHSIIEPGHDLQTSYAFIRIHQDNAKPLFSFLQNLDFIESIIPYHDTELSDDLHKLISISKSKILEAPKQYELYNLSNLTNNPKQSLYFAFLQNYIKWLIPFSFFGLSIRFLSNFTYEFNSTYSLFAILWTLSFTAFWLYKYEPFWSDRLSKYSSFSTIEFLQDKQKAQKKASSVIMLKKCCFIPVALLFGAILLSFQLYCFALEIFIKQIYNGPMISILSFLPTILICTFTPVLTVIYNKYFVEPMTKWENHSSVVNAKKSKEAKNFVIIFLSSYVPLLITLFLYLPMGHLLTAEIRTKVFNAFSILARLPTHDSDFIIDTKRYEDQFFYFIVINQLIQFSMENFVPSLVSIAQQKINGPNPNFVKAESEIGKAQLSSSDMKIWSKVKSYQTDPWGATFDLDANFKKLLLQFGYLVMFSTIWPLAPFICLIVNLIVYQVDLRKAVLYSKPEYFPFPIYDKPSSVSNTQKLTVGLWNSVLVMFSILGCVITATLTYMYQSCNIPGVGAHTSIHTNKAWYLANPINHSWINIVLYAVFIEHVSVAIFFLFSSILKSSHDDVANGIVPKHVVNVQNPPKQEVFEKIPSPEFNSNNEKELVQRKGSANEKLHQELGEKQPASSANGYEAHAATHANNDPSSLSSASSPSLSSSSSSSKTGVVKAVDNDTAGSAGKKPLATESTEKRNSLVKVPTVGSYGVAGATLPETIPTSKNYYLRFDEDGKSIRDAKSSAESSNATNNNTLGTESKLLPDGDAVDALSRKIDQIPKIAVTGGENNENTQAKDDAATKTPLIKDANIKPVVNAAVNDNQSKVSVATEQTKKTEVSTKNGPSRSISTKETKDSARPSNNNTTTTTTTDATQPHHHHHHHRHRDAGVKNVTNNSKTTESSSSSSAAKEKPKHKKGLLHKLKKKL btn2-ist2 Interaction detected by CoIP 499477 N/A 499479 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 499480 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 1 btn2-ist2-1 Interaction detected by Y2H 499478 N/A 499480 unspecified role unspecified role prey prey N/A 499479 unspecified role unspecified role bait bait physical association physical association false false false None