IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ luo-2005-1 Mutations in the spacer region of Drosophila mitochondrial DNA polymerase affect DNA binding, processivity, and the balance between Pol and Exo function. spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Luo N., Kaguni LS. Reconstituted holoenzymes purified by phosphocellulose chromatography. Fig 2A lskaguni@msu.edu J. Biol. Chem. (0021-9258) 2005 dpog2_drome DNA polymerase subunit gamma-2, mitochondrial DNApol-gamma35 MtPolB DNA polymerase beta subunit DNA polymerase gamma 35kD subunit DNApol-gamma DNApolG2 l(2)34De l(2)br16 pol gamma-beta CG33650 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSRIQRCFKSLASAGFFRTVEDNKLELLSHGREYAKLLQQHWTRLRPLAAHLGATKEPINPVNIQRFSFPQSQQFRNNFQKLVKDHPRKAKCPTLLKHQSTCSGPTSNSLFGIKGPTLHLTTDFLVEPHRALEHFYNMQRESKIWWMRLSSNPSRYRIVPCDLAEDLNPNDYQAIDIRTSYGDAGEVTVEQLSLVRIVDDKDFRLPDARTGEIVQPTVIRSVIELETTTCALLLDGCDHGRDSQSLLLHRVLAPYQCGIACVESDSELSADLSDLCQHLKHVLNHAGLRLSEGDGIRTTKNASHLAEHLLETDMLGIPYTLVINEQTLRNGLMQLRSRDTRLAETIHISDVPDYLLNIFKN dpog1_drome DNA polymerase subunit gamma-1, mitochondrial tam MtPolA Polg Mitochondrial DNA polymerase catalytic subunit 3'-5' exodeoxyribonuclease DNApol-gamma DNApol-gamma125 DNApolG1 l(2)34Dc l(2)br5 pol gamma-alpha CG8987 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQFHLIRKYASKVSREHYASSSVKIFRRVKPPQKVNKPKKPENVENGPTEYAENLVKVQMISRNLHAQLFPQAPRSISEQQVASAKVYKDELRRHGVDIESSAPVSDVQLKLPALRGANIEEHFHNIAKEQVQPYEELLLPLVQCEQLPKRPKRWAFHTGWTAYDPEDGTATPVDHPLEKGLVFDVEVCVSEGQAPVLATAVSTKRWYSWVSSKLTKHRLSVEKLEPLDVDTDSERPHYTTDELIPLGTTGPGLVVGHNVSYDRARLKEQYLTEDTGTRFVDTMSLHMCVSGVTSYQRAMLKSKKEPAAEDLGWLEQSSLNSLVEVHRLYCGGDTLSKEPRNIFVEGTLEQVRQSFQSLTNYCASDVEATHRILRVLYPLYAERFPHPASLAGMLEMGSAYLPVNSNWERYIREAQLTYEDLSIEAKYHLGRRAEEACSLLLDDQYRQNLWLWDEDWSVQELKLKQPPKRKPLPTVELKDSGNTPEERRLQAKFQHLYDQQALLPARRPLLPGYPLWYRKLCRKPPAKRADEILEDDEEPWSPGASEISTGMQIAPKLLSLCWEGYPLHYEREQGWGFLVPFRSDSEGVDRLPMEQLLAHCPVPEFARLSASKAESDMAFDMLPGQVEQHLGKREHYKKLSQKQQRLETQYQGSGVWCNKVLDDCCFFLKLPHKNGPSFRVGNPLSKDFLNKFAENVLSSGDPSCQAAARVIDIARMMSYWRNNRDRIMGQMVVWLDSQQLPNEFTGEKCQPIAYGAICPQVVACGTLTRRAMEPTWMTASNSRPDRLGSELRSMVQAPPGYRLVGADVDSQELWIASVLGDAYACGEHGATPLGWMTLSGSKSNGSDMHSITAKAVGISRDHAKVINYARIYGAGQLFAETLLRQFNPTFSASEAKAKAMKMFSITKGKRVYRLREEFHDELEDRAYSSYEASRLAIQRNRTLAEVFHRPNWQGGTESAMFNRLEEIATGSQPRTPFLGGRLSRALEADTGPEQEQRFLPTRINWVVQSGAVDFLHLMLVSMRWLMGSHVRFCLSFHDELRYLVKEELSPKAALAMHITNLMTRSFCVSRIGLQDLPMSVAFFSSVEVDTVLRKECTMDCKTPSNPHGLRIGYGIQPGQSLSVAEAIEKAGGNDVSQWDWIKKS tam-dnapol-gam-35-1 Interaction between drosophila DNA polmerase gamma subunit 1 and 2 identified by affinity chromatography 332232 N/A 332233 unspecified role unspecified role neutral component neutral component N/A 332234 unspecified role unspecified role neutral component neutral component physical association physical association false false false Resides 483�533 of tam required for catalytic activity