IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gates-2004-1 rigor mortis encodes a novel nuclear receptor interacting protein required for ecdysone signaling during Drosophila larval development. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Gates J., Lam G., Ortiz JA., Losson R., Thummel CS. Bound in vitro synthesized 35S-labelled protein was resolved by SDS-PAGE and visualized by autoradiography. carl.thummel@genetics.utah.edu Development (0950-1991) 2004 7up1_drome Steroid receptor seven-up, isoforms B/C svp NR2F3 CG11502 Nuclear receptor subfamily 2 group F member 3, isoforms B/C protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MCASPSTAPGFFNPRPQSGAELSAFDIGLSRSMGLGVPPHSAWHEPPASLGGHLHAASAGPGTTTGSVATGGGGTTPSSVASQQSAVIKQDLSCPSLNQAGSGHHPGIKEDLSSSLPSANGGSAGGHHSGSGSGSGSGVNPGHGSDMLPLIKGHGQDMLTSIKGQPTGCGSTTPSSQANSSHSQSSNSGSQIDSKQNIECVVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLTYSCRGSRNCPIDQHHRNQCQYCRLKKCLKMGMRREAVQRGRVPPTQPGLAGMHGQYQIANGDPMGIAGFNGHSYLSSYISLLLRAEPYPTSRYGQCMQPNNIMGIDNICELAARLLFSAVEWAKNIPFFPELQVTDQVALLRLVWSELFVLNASQCSMPLHVAPLLAAAGLHASPMAADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCLKAIVLFTTDACGLSDVTHIESLQEKSQCALEEYCRTQYPNQPTRFGKLLLRLPSLRTVSSQVIEQLFFVRLVGKTPIETLIRDMLLSGNSFSWPYLPSM rig_drome Protein rigor mortis rig CG30149 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNSQVMYHNVPTPPGNVSLAAAAPDGGLLYAGIRCINYISAPPANGEQPQVVTMSTRINILALDVSPMWGLGNGGPTKPFAIVGDDLSVQVWDCALGEAVIGHKAHQHQHEARDVRVVHHTTNSVLMSYLANGNILSMDASDLVIYCVASNTYCRRSTFISPRNHQLTMVRCSPYNDNLFAVGTAMGNVLVCDLRKMNIVYKFHGHKAPICGLAWREVPAAEDEKTNNLALSAEEWRSRNGGQEEKPKTKPPPLTKSKAAESDDPFDIYNFDHLEYEFGAPIAERRRKSSEDCGGEFVGLEKPAGAAVLDFVEACESVKAELLASRQEDKTQHVEVTLHDCEPTKPTGPLSDASTISNKNDASDSTEGSLEVIQYSSSSDDAVIVDGEAAKPKREVLHHIYHQAEVHASGTPQTKSEPQSNLQVVPAISAETISLTSVNSTHLETLLVSIDGDEVMMIWNTNTGAHAGKNYSKSKTAGKLNNVYWLNNHVIVSLSRHQLFFWSVEFERKMLRYKISKDKSHSCHLQDIVSFACDSSKEMIWLCRNNRQIGMMNPKTGRMADFYGTVAFGVRAMAECPDDMNKIALGCSDRRVAFFDISKLTTSCLPIDSVYVSSNVYCLAWSPNCLELAFGTFDGTVGILDVERMKVKTHLRTPHKKEVYSLVWQDHFIYFIVNRVLGFFDLRKSKIEPTIVNCISRPSYLSIRDSFLFVGTDDGLLQIHERDSGMEKSWSPFIRQSALFARYVTDIAWCPLDSNKFAVSGNDRSVYVMEFQPTERNWKTLHTFTANTEKASITSMRWSHTQKHLLLTFHIEGKVCLWNCNAPEKPPLTITYHCPMWCGMFLPTNENIIMCSGKALSVELIDIKDALEGDEKSICPKVDALLNVKWASKSLTQPYAPVLTAAEKKRQRRDQRKAAAKLEVDVANKDQKKIQESVTAVIDNPTNDKCTQETPVEEMLEALSLDKEQNNRSAKECPKCKEQSPDSFTHSRTCLYLTQKELNKSALEKLAIVLTEDSAKIDKSVLISKLFSSKVMAKELIATELTNLKHSNTKDIAPLCLAMSTFKLREELEQHIANKTLTERHVSLAPSVSFTLWQDCCRAYAKQMEEKGYIMHAATYLFSQGMQSEAIKLFLANEYYKEALVHARICLPATDPLIKTVINNWLEHLEGTGNFAAAALICVLDNEMLRGYSYLRKYRNCTPEIADLMDQIKRIGQLGGVLDGCAPNEPIHNGSTAEH usp_drome Protein ultraspiracle usp Cf1 NR2B4 CG4380 XR2C Chorion factor 1 Nuclear receptor subfamily 2 group B member 4 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDNCDQDASFRLSHIKEEVKPDISQLNDSNNSSFSPKAESPVPFMQAMSMVHVLPGSNSASSNNNSAGDAQMAQAPNSAGGSAAAAVQQQYPPNHPLSGSKHLCSICGDRASGKHYGVYSCEGCKGFFKRTVRKDLTYACRENRNCIIDKRQRNRCQYCRYQKCLTCGMKREAVQEERQRGARNAAGRLSASGGGSSGPGSVGGSSSQGGGGGGGVSGGMGSGNGSDDFMTNSVSRDFSIERIIEAEQRAETQCGDRALTFLRVGPYSTVQPDYKGAVSALCQVVNKQLFQMVEYARMMPHFAQVPLDDQVILLKAAWIELLIANVAWCSIVSLDDGGAGGGGGGLGHDGSFERRSPGLQPQQLFLNQSFSYHRNSAIKAGVSAIFDRILSELSVKMKRLNLDRRELSCLKAIILYNPDIRGIKSRAEIEMCREKVYACLDEHCRLEHPGDDGRFAQLLLRLPALRSISLKCQDHLFLFRITSDRPLEELFLEQLEAPPPPGLAMKLE hr3_drome Probable nuclear hormone receptor HR3 Hr3 Hr46 Nuclear receptor subfamily 1 group F member 4 CG11823 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MYTQRMFDMWSSVTSKLEAHANNLGQSNVQSPAGQNNSSGSIKAQIEIIPCKVCGDKSSGVHYGVITCEGCKGFFRRSQSSVVNYQCPRNKQCVVDRVNRNRCQYCRLQKCLKLGMSRDAVKFGRMSKKQREKVEDEVRFHRAQMRAQSDAAPDSSVYDTQTPSSSDQLHHNNYNSYSGGYSNNEVGYGSPYGYSASVTPQQTMQYDISADYVDSTTYEPRSTIIDPEFISHADGDINDVLIKTLAEAHANTNTKLEAVHDMFRKQPDVSRILYYKNLGQEELWLDCAEKLTQMIQNIIEFAKLIPGFMRLSQDDQILLLKTGSFELAIVRMSRLLDLSQNAVLYGDVMLPQEAFYTSDSEEMRLVSRIFQTAKSIAELKLTETELALYQSLVLLWPERNGVRGNTEIQRLFNLSMNAIRQELETNHAPLKGDVTVLDTLLNNIPNFRDISILHMESLSKFKLQHPNVVFPALYKELFSIDSQQDLT ecr_drome Ecdysone receptor EcR NR1H1 CG1765 Ecdysteroid receptor 20-hydroxy-ecdysone receptor EcRH Nuclear receptor subfamily 1 group H member 1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKRRWSNNGGFMRLPEESSSEVTSSSNGLVLPSGVNMSPSSLDSHDYCDQDLWLCGNESGSFGGSNGHGLSQQQQSVITLAMHGCSSTLPAQTTIIPINGNANGNGGSTNGQYVPGATNLGALANGMLNGGFNGMQQQIQNGHGLINSTTPSTPTTPLHLQQNLGGAGGGGIGGMGILHHANGTPNGLIGVVGGGGGVGLGVGGGGVGGLGMQHTPRSDSVNSISSGRDDLSPSSSLNGYSANESCDAKKSKKGPAPRVQEELCLVCGDRASGYHYNALTCEGCKGFFRRSVTKSAVYCCKFGRACEMDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGGNGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIYKLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAFTKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIEDLLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHCGDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQSHLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSLTQNDSQHQTQPQLQPQLPPQLQGQLQPQLQPQLQTQLQPQIQPQPQLLPVSAPVPASVTAPGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGGNVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA ftf1b_drome Nuclear hormone receptor FTZ-F1 beta Hr39 FTZF1-beta FTZ-F1-beta NR5-beta-1 CG8676 NR5B1 Nuclear hormone receptor HR39 Nuclear receptor subfamily 5 group B member 1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPNMSSIKAEQQSGPLGGSSGYQVPVNMCTTTVANTTTTLGSSAGGATGSRHNVSVTNIKCELDELPSPNGNMVPVIANYVHGSLRIPLSGHSNHRESDSEEELASIENLKVRRRTAADKNGPRPMSWEGELSDTEVNGGEELMEMEPTIKSEVVPAVAPPQPVCALQPIKTELENIAGEMQIQEKCYPQSNTQHHAATKLKVAPTQSDPINLKFEPPLGDNSPLLAARSKSSSGGHLPLPTNPSPDSAIHSVYTHSSPSQSPLTSRHAPYTPSLSRNNSDASHSSCYSYSSEFSPTHSPIQARHAPPAGTLYGNHHGIYRQMKVEASSTVPSSGQEAQNLSMDSASSNLDTVGLGSSHPASPAGISRQQLINSPCPICGDKISGFHYGIFSCESCKGFFKRTVQNRKNYVCVRGGPCQVSISTRKKCPACRFEKCLQKGMKLEAIREDRTRGGRSTYQCSYTLPNSMLSPLLSPDQAAAAAAAAAVASQQQPHQRLHQLNGFGGVPIPCSTSLPASPSLAGTSVKSEEMAETGKQSLRTGSVPPLLQEIMDVEHLWQYTDAELARINQPLSAFASGSSSSSSSSGTSSGAHAQLTNPLLASAGLSSNGENANPDLIAHLCNVADHRLYKIVKWCKSLPLFKNISIDDQICLLINSWCELLLFSCCFRSIDTPGEIKMSQGRKITLSQAKSNGLQTCIERMLNLTDHLRRLRVDRYEYVAMKVIVLLQSDTTELQEAVKVRECQEKALQSLQAYTLAHYPDTPSKFGELLLRIPDLQRTCQLGKEMLTIKTRDGADFNLLMELLRGEH rig-svp 566314 N/A 566315 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 566316 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false 5A rig-usp 566314 N/A 566316 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 566317 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 5A rig-hr3 566314 N/A 566318 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 566316 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false 5A rig-ecr 566314 N/A 566319 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 566316 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false 5A This interaction was also observed in the presence of ligand muristerone A. rig-hr39 566314 N/A 566320 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 566316 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false 5A rig-hr39-2 566314 N/A 566320 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 566316 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false 5B