UniProt UniProt http://www.uniprot.org desai-2003-3 KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. fluorescence imaging fluorescence microscopy fluorescence imaging polyclonal immunost polyclonal antibody immunostaining polyclonal immunost Desai A., Rybina S., Muller-Reichert T., Shevchenko A., Shevchenko A., Hyman A., Oegema K. koegema@ucsd.edu Genes Dev. (0890-9369) 2003 hyman@mpi-cbg.de desai-2003-2 KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Desai A., Rybina S., Muller-Reichert T., Shevchenko A., Shevchenko A., Hyman A., Oegema K. koegema@ucsd.edu Genes Dev. (0890-9369) 2003 hyman@mpi-cbg.de desai-2003-1 KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Desai A., Rybina S., Muller-Reichert T., Shevchenko A., Shevchenko A., Hyman A., Oegema K. koegema@ucsd.edu Genes Dev. (0890-9369) 2003 hyman@mpi-cbg.de nuf2_caeel Kinetochore protein Nuf2 homolog R12B2.4 Kinetochore protein him-10 Protein high incidence of males 10 him-10 protein protein caeel Caenorhabditis elegans MSNVVLIVYDPRMISKYLGQKLHMGLVADDIIKPTAEIAQQIFANFVRLVLNVSESSLTTLPLSANCDYDPELHKKSIPIIILFQCMKAFIKDNSGNKLDLTMCDLVTPAKHEHRFRKLTSFLVDFLKLHELATPAFNEISEEFSDRKFEMEKIREELLEAEKKKNDLLAKQSIRKRHEHELINEQSNAKAELKNVVNEYTETRQINEELDKQKEEAILHIQALEKEMLTGKKTIEHLNEEVLTSPEQLKQEMEERKRHIEELRDCLESSKKGLQAKLEAREICINSEKNVPVIIEKIHQWTEVREVIIDLIDVESENLRKLKEMEEQLDFMMKEMETAQKRLVEQSETHEQLRIEHTQKSEERQRRIEEITEQIANLKTSQPDVSQEIAKKKQELLALKNAHSETISQITNSCQDAVAKFAKLNAMFKETQKVAFEKNTAAAREMERLKSSLTGRLLSDYTFGSSTIDAGENTENCDPQPNDSSFSVFK ndc80_caeel Kinetochore protein ndc-80 ndc-80 W01B6.9 Hec1 homolog protein protein caeel Caenorhabditis elegans MFGDRRKTGGLNLNGRASIAITPTKRFTDYTGSTSVRKTDARPSLSQPRVSLFNTKNSSVAPRDVKSLVSLNGSKIYNFLVEYESSDAPSEQLIMKPRGKNDFIACFELIYQHLSKDYEFPRHERIEEEVSQIFKGLGYPYPLKNSYYQPMGSSHGYPHLLDALSWLIDIIRINSAVSEDTQNILFGDFMEQGKAQEKTLNYAWMTSTFRDYTNDRKAAENPSSSYWDDTKHRLRKYFEQSNEFEDMTKTAASALEMLNYECDEIEADKGNEASLKEEISRIRDDIRKAKDYLEQNLHVKQHMEKELAMVKSEQEEKISENEKVQKMVDDLKNKIELQKQIHGLTGKEVRQMNLDNNKDKEVVLEIQSELDRLSKETWKLKDEDFFKEQKSKFIHLAEQIMKILSGLNIQMNLEPLRAPTNERDLKDYWETLNKIWVPEISRQLHQRKLELETEQSRFSNKAVTAEERIQIQSETLCEAKKNEAREERIRRNERDSWKDARKHIEQRYEQLLNEKEVLLKQMKLDGSLEKEIEDETARMSATGEEHIQKRSQLEAGIRQILDLMVVEIAEIENKKIGFHVQCAGIEKAVL g5eda3_caeel hcp-4 CELE_T03F1.9 T03F1.9 protein protein caeel Caenorhabditis elegans MNRKPRTRSTIVPGRKMITEIVALREAGLDDTRPSYLEEPTVIVDESMMNDSANLEEREWRKQGLSEKQMFAILEKRKQTQLNNARKRLEKEMYGAKTFKELIACREYSECESDSENTVNQNVRSASVFAIPALPKHISEKSMMGSPIANSRGSGKAGLSCSTPKSSSDTSMRSLRSLDISHVVNTDRLDAERVTVHTKSVVIPTILEERESTLQKTLTIEKNHSSQLQKTFTVAEDAPEELQKTVTIEKNQSSEMQRTFTVAKDASKEQSQLQRTVTIEKNQSSQLQGNFTVAKDAPEELQKTVTIEKNQSSEMQGTVTVAKDAEHSSLQKTFTKPTEKDESSLQRTFNVANRDENNDSTLQKTFIIEERDAYEQGTTSVGVKPPVLAQNTMEGGEKRRTSKVTSDERERRNASISNPLHNSMLEKEEIQSPGANFFKHPRKKIRPVVQTPPRIKATSRLSTESNKERTIEMESVAEERTMEADSIGSSYISPTFNASRVSPVPEVPEKVEPMHVSKAATPKSIRHTDNSIRNIGPTNNVECARAALLSTPTRMDIVDSVNRVTPARRYEQPTFASLVKRMNMKDANRLLEETSRKNTPAKTTATTSSAAVRMVLEDDEEDQATEVIEKRSENGGVIVDGEDEAADSSNRSLNIELNALTVNEEPAHDISAIDFPEDDTNEMRSSSDEEEMEARPDPRKKSSRRLGLLSDSIALGLASSSRRRPNDTFVDETWYPEPNSKGNRRPRTHRGMKLKEHQLMKPEDAPDGVRRSTRVRVKPVRSWLGEQPVYVNSPISGCKRLTGVTAVVIKDPRLCYYRTADVRTATERELKDKANKRALAQEKKQQRQNARSGRRHDSDDDEEDDM bub1_caeel Mitotic checkpoint serine/threonine-protein kinase bub-1 bub-1 Budding uninhibited by benzimidazole 1 R06C7.8 protein protein caeel Caenorhabditis elegans MSHIRVAFAPLDTNPSTCGLETFATQIETLSQMMTAEEMKAELYDCVRRILREQKFDQIRENEAMLRLYKVMGRSSTNLKGRGIYEQLFKKDHFTGSLKFYLQWAEECGKDQMLEEFKDVLKLARDRLSERIEMTAIESGFRDLVDEYFNGESGDMFTRPDETMDLFRFNAGSKKKRRSSVCFLQHNVPINNSGKAAFGPKTKTDLRQACIDRPNYHGISIEEFRFAKWKDTFGEDVDDDYRKRKDSGVVFVKHQVIDTDRQAREEVENRFNANLNPRRRHLSPVSEKTVDDEEEKRSRIYSPLVATKDAHRPALRSKIENPPATVTLSSDTKSASEKDVSDSDDADDDERLKIMTAGRKDGNPPDRSTSISSNYSTASARTSKSGAGLDLMAENKCLEAHAMFSDTVHLASEKTMVLGDDSVFVPERSLATTQIVTDFSVLCDPDPTMTITQERPKKVSNGLNVVYDEAAEPEESQKVEESEVQPEIVLVSPVTQTSPATMFNDIYDDEIEFGFFKPSRGNFVTSTPAQGVHLVNIDEYFGNKEEESTHEQEAPVFVAPTSSTFSKLTRRKSLAANQAVQPSVTESSKPERSDPKDSSIDCLTANLGRRLSIGADEIPNLTENNESEITGCKIRRRSEIIKQGDINPWDETLRKKLMCLVRPPQNMHEFQERAPKIQALRDCEVSGEKLHIQTLIGQGGYAKVYRAVTDDQRTVAVKYEVPSCSWEVYICDQMRNRLKDRGLELMAKCCIMEVMDAYVYSTASLLVNQYHEYGTLLEYANNMKDPNWHITCFLITQMARVVKEVHASKIIHGDIKPDNFMITRKIDDKWGKDALMSNDSFVIKIIDWGRAIDMMPLKNQRFKGRAGTEAFDCPEMVDGRSWTYQADYFGFAATMAVVVAGKYAQLTGASVGDYSLNVDIKRRNILRDACYDVINRFLNIPSCDSLPDWNILIKSFSEIWNEKFEASGWRQAVSKFNEACDLAANQK knl1_caeel Kinetochore null protein 1 C02F5.1 knl-1 protein protein caeel Caenorhabditis elegans MSMEPRKKRNSILKVRQAVETIEETVMNSGPSSTTTNRRVSFHNVKHVKQYDRDHGKILDATPVKEKITDTIGSDGILTPRGGNMDISESPACTSSFQVFGGGNLDKTMDMSLETTINENNETARLFETTRDPTLLYEKIVETTTKVTERIVSMPLDDTLAMFNTTNQEDKDMSVDRSVLFTIPKVPKHNATMNRTIPMDLDESKAAGGQCDETMNVFNFTNLEAAEMDTSKLDENNTMNAIRIPINSNVMPVDMDITEHHTLIEEKKNDTFGPSQLMDISAPQVQVNDTLAIFNSPRDICNKGLGVPQNLINIASNVVPVDMDITDQAVLNAEKKNDQFETSQLMDISIPKVLVNDTMAMFNSPKHVSKSSMDLEKTIEAADKSTKYPSIADEVEDLDMDMDITEQQPCEAGNQQNDGLQLQKEDLMDISVIRDSPAVNDTMAVFQSPARVKIGANNSIIDSQKSIVFGDEMSIDETQNDGTLTLPKSNVEVTTTNDVYTSLERQEENASENVSMINESSVHSEIDKKSFMLIEEERAFMHSSMIDVAQKLEDDGSSKTPVILASQSASLATKEPSALHNSSATLNNSMELDNNTLLKTMQITTCEDISMVHESIAVELNSNKEQEQFGDETLQKNDTSNTGANFTFQGHNETSQIMNNVDSEAVNTSKISTYSAFNLSINQSISKRRRSLLNSARESPRRVALENSIMSMNGQTMEALTEYRQNKTMQTSQDSMPSMSLNDSGRDILAMNTSVRSPHLNSSKTAAPGTPSLMSQNVQLPPPSPQFEMPDFDPAVVNVVYLTSEDPSTEQHPEALKFQRIVENEKMKVQHEIDSLNSTNQLSAEKIDMLKTKELLKFSHDEREAIMIARKDAEIKFLELRLKFALEKKIESDQEIAELEQGNSKMAEQLRGLDKMAVVQKELEKLRSLPPSREESGKIRKEWMEMKQWEFDQKMKALRNVRSNMIALRSEKNALEMKVAEEHEKFAQRNDLKKSRMLVFSKAVKKIVNF ndc-80_him-10_1 colocalization of ndc-80 and him-10 by immunofluorescence microscopy 507756 N/A 507759 unspecified role unspecified role neutral component neutral component N/A 507760 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 5A hcp-4_knl-1_3 colocalization of bub-1, hcp-4 and knl-1 by immunofluorescence microscopy 507756 N/A 507761 unspecified role unspecified role neutral component neutral component N/A 507762 unspecified role unspecified role neutral component neutral component N/A 507763 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 7B hcp-4_knl-1_2 colocalization of hcp-4 and knl-1 by immunofluorescence microscopy 507756 N/A 507761 unspecified role unspecified role neutral component neutral component N/A 507763 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 2C knl-1_ndc-80_2 coIP: bait=knl-1, preys=hcp-4, him-10, ndc-80 507757 N/A 507761 unspecified role unspecified role prey prey N/A 507759 unspecified role unspecified role prey prey N/A 507760 unspecified role unspecified role prey prey N/A 507763 unspecified role unspecified role bait bait association association false false false Fig. 4C hcp-4_knl-1_1 coIP: bait=hcp-4, preys=knl-1, ndc-80 507757 N/A 507761 unspecified role unspecified role bait bait N/A 507760 unspecified role unspecified role prey prey N/A 507763 unspecified role unspecified role prey prey association association false false false Fig. 4D knl-1_ndc-80_1 coIP: bait=knl-1, preys=ndc-80, him-10 507758 N/A 507763 unspecified role unspecified role bait bait N/A 507760 unspecified role unspecified role prey prey N/A 507759 unspecified role unspecified role prey prey association association false false false Fig. 4A