IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hebert-1992-1 Glucose transporter oligomeric structure determines transporter function. Reversible redox-dependent interconversions of tetrameric and dimeric GLUT1. human-erythrocyte Homo sapiens erythrocyte Erythrocyte, Red blood cell; Corpuscle; RBC molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column elisa enzyme linked immunosorbent assay ELISA ELISA elisa Hebert DN., Carruthers A. TC@UMASSMED.UMMED.EDU J. Biol. Chem. (0021-9258) 1992 Only protein-protein interactions imex curation hebert-1992-2 Glucose transporter oligomeric structure determines transporter function. Reversible redox-dependent interconversions of tetrameric and dimeric GLUT1. human-erythrocyte Homo sapiens erythrocyte Erythrocyte, Red blood cell; Corpuscle; RBC cosedimentation cosedimentation elisa enzyme linked immunosorbent assay ELISA ELISA elisa Hebert DN., Carruthers A. TC@UMASSMED.UMMED.EDU J. Biol. Chem. (0021-9258) 1992 Only protein-protein interactions imex curation hebert-1992-3 Glucose transporter oligomeric structure determines transporter function. Reversible redox-dependent interconversions of tetrameric and dimeric GLUT1. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column elisa enzyme linked immunosorbent assay ELISA ELISA elisa Hebert DN., Carruthers A. Density of GLUT1 on cell surface is 10-15 times lower than on human erythrocytes suggesting that GLUTl oligomeric size is independent of cell GLUTl content TC@UMASSMED.UMMED.EDU J. Biol. Chem. (0021-9258) 1992 Only protein-protein interactions imex curation gtr1_human Solute carrier family 2, facilitated glucose transporter member 1 Glucose transporter type 1, erythrocyte/brain HepG2 glucose transporter SLC2A1 GLUT1 protein protein human Homo sapiens Human MEPSSKKLTGRLMLAVGGAVLGSLQFGYNTGVINAPQKVIEEFYNQTWVHRYGESILPTTLTTLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLMMNLLAFVSAVLMGFSKLGKSFEMLILGRFIIGVYCGLTTGFVPMYVGEVSPTALRGALGTLHQLGIVVGILIAQVFGLDSIMGNKDLWPLLLSIIFIPALLQCIVLPFCPESPRFLLINRNEENRAKSVLKKLRGTADVTHDLQEMKEESRQMMREKKVTILELFRSPAYRQPILIAVVLQLSQQLSGINAVFYYSTSIFEKAGVQQPVYATIGSGIVNTAFTVVSLFVVERAGRRTLHLIGLAGMAGCAILMTIALALLEQLPWMSYLSIVAIFGFVAFFEVGPGPIPWFIVAELFSQGPRPAAIAVAGFSNWTSNFIVGMCFQYVEQLCGPYVFIIFTVLLVLFFIFTYFKVPETKGRTFDEIASGFRQGGASQSDKTPEELFHPLGADSQV gtr1-1 Homotetramization of GLUT1 demonstrated by size exclusion chromatography 491920 N/A 491923 unspecified role unspecified role neutral component neutral component Stoichiometry: 4.0 physical association physical association false false false Average Stokes radius = 7.7 & 0.1 nm. 3 GLUT1 is tetrameric in non-reducing conditions, dimeric in the presence of 5mM DTT gtr1-2 Homotetramization of GLUT1 demonstrated by size exclusion chromatography 491921 N/A 491923 unspecified role unspecified role neutral component neutral component Stoichiometry: 4.0 physical association physical association false false false Molecular mass of tetramer = 210kDa 5 GLUT1 is tetrameric in non-reducing conditions, dimeric in the presence of 5mM DTT gtr1-3 Homotetramization of GLUT1 demonstrated by size exclusion chromatography 491922 N/A 491923 unspecified role unspecified role neutral component neutral component Stoichiometry: 4.0 physical association physical association false false false Average Stokes radius = 7.8 nm. 8 GLUT1 is tetrameric in non-reducing conditions, dimeric in the presence of 5mM DTT