IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nelson-2003-1 RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nelson B., Kurischko C., Horecka J., Mody M., Nair P., Pratt L., Zougman A., McBroom LD., Hughes TR., Boone C., Luca FC. Plasmids encoding positive interactors were sequenced. fluca@vet.upenn.edu,charlie.boone@utoronto.ca fluca@vet.upenn.edu or charlie.boone@utoronto.ca Mol. Biol. Cell (1059-1524) 2003 Only protein-protein interactions imex curation nelson-2003-2 RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nelson B., Kurischko C., Horecka J., Mody M., Nair P., Pratt L., Zougman A., McBroom LD., Hughes TR., Boone C., Luca FC. Plasmids encoding positive interactors were sequenced. fluca@vet.upenn.edu,charlie.boone@utoronto.ca fluca@vet.upenn.edu or charlie.boone@utoronto.ca Mol. Biol. Cell (1059-1524) 2003 Only protein-protein interactions imex curation nelson-2003-3 RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nelson B., Kurischko C., Horecka J., Mody M., Nair P., Pratt L., Zougman A., McBroom LD., Hughes TR., Boone C., Luca FC. Plasmids encoding positive interactors were sequenced. fluca@vet.upenn.edu,charlie.boone@utoronto.ca fluca@vet.upenn.edu or charlie.boone@utoronto.ca Yeast cDNA library derived from pACT was used. Mol. Biol. Cell (1059-1524) 2003 Only protein-protein interactions imex curation kic1_yeast Serine/threonine-protein kinase KIC1 Kinase that interacts with CDC31 N-rich kinase 1 YHR102W KIC1 NRK1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTTKPQNSKQGLAEGEMDVSSLFKRTEVIGRGKFGVVYKGYNVKTGRVYAIKVLNLDSDSDEVEDVQREIQFLASLKQISNITRYYGSYLKDTSLWIIMEHCAGGSLRSLLRPGKIDEKYIGVIMRELLVALKCIHKDNVIHRDIKAANVLITNEGNVKLCDFGVAAQVNQTSLRRQTMAGTPYWMAPEVIMEGVYYDTKVDIWSLGITTYEIATGNPPYCDVEALRAMQLIIKSKPPRLEDRSYSTSLKEFIALCLDEDPKERLSADDLLKSKFIRAHKATPTSILKELISRYLLFRDKNKNKYKIEGSIPENEPSKPSEAPKPSQNGGGDEAQKSIASNDNEIKRVNEGDVEMKWDFDSLSSSDYIIENNINLDALAEDNNEWATAQHDLFNYAYPDEDSYYFDPTSHNTRPFVYQGTTIGKGYPGTIAQNSTLNAPVTNNYTNSKYPSKMVAGTTNTSGTHTAGPMTSSKRLESKAPKQLLELFEDNEIITAENDVNTEAPKISKSISSLNAGNSSRDDFIPSISNEVNGNINNNKMRPHLPPLSSGNNYYSQSTPALPLLQTKFNKTSKGPPTSGLTTAPTSIEIEIPEELPNSALPTPASADPVLIPSTKARSSTVTAGTPSSSSSIQYKSPSNVPRRLTVSNNRPEHCPSTITNQKLGSAVASNSGISSTPNNSNNYNNNTDSENSRGSSGSNTANSTQMGITNPGNVTKLSTHKASSPSRPLFGVGTSPNRKPAGSPTQNIGHNSTHTNLAPPPTMKPMANSKDNKDILLQPLNSIPSSSTLNTISGNSSNNLTSSNYFSNEKEGSRVNGDFKRNNPNLKLQMPLPTPVVRNKLLDPNTATSQNNNGMPGSAGISTNENINQFGFNTSSASNIPVSMTPISEKHIDFGGKIKRSQSISNRKNSSASEHPLNILGSSVSGNVSGIGNNNVGSNNNSGPNNSVPLSANTGNTTIKANSTTIATSSSAAASTTAPISQQTIPSGTQFNHILSSAATAANSVNSLGFGMCPPPQSLQMEMFLDLESFLPGKQRRVDRKPQVLKELENLLQMFEEGLPCIEHALKEQLISTPIKDNEH 9989EAF315EE0B94 hym1_yeast Protein HYM1 YKL189W HYM1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFKKYKNQDLDMAFWWKKNPKTPSDYARLIIEQLNKFSSPSLTQDNKRKVQEECTKYLIGTKHFIVGDTDPHPTPEAIDELYTAMHRADVFYELLLHFVDLEFEARRECMLIFSICLGYSKDNKFVTVDYLVSQPKTISLMLRTAEVALQQKGCQDIFLTVGNMIIECIKYEQLCRIILKDPQLWKFFEFAKLGNFEISTESLQILSAAFTAHPKLVSKEFFSNEINIIRFIKCINKLMAHGSYVTKRQSTKLLASLIVIRSNNALMNIYINSPENLKLIMTLMTDKSKNLQLEAFNVFKVMVANPRKSKPVFDILVKNRDKLLTYFKTFGLDSQDSTFLDEREFIVQEIDSLPRIISSTTEVSNNNASSSNVASITSPSSVMNNQSSILTHSTSPDSR F48860754C892BA9 cbk1_yeast Serine/threonine-protein kinase CBK1 CBK1 YNL161W N1727 Cell wall biosynthesis kinase protein protein yeast Saccharomyces cerevisiae Baker's yeast MYNSSTNHHEGAPTSGHGYYMSQQQDQQHQQQQQYANEMNPYQQIPRPPAAGFSSNYMKEQGSHQSLQEHLQRETGNLGSGFTDVPALNYPATPPPHNNYAASNQMINTPPPSMGGLYRHNNNSQSMVQNGNGSGNAQLPQLSPGQYSIESEYNQNLNGSSSSSPFHQPQTLRSNGSYSSGLRSVKSFQRLQQEQENVQVQQQLSQAQQQNSRQQQQQLQYQQQQQQQQQQQHMQIQQQQQQQQQQQQSQSPVQSGFNNGTISNYMYFERRPDLLTKGTQDKAAAVKLKIENFYQSSVKYAIERNERRVELETELTSHNWSEERKSRQLSSLGKKESQFLRLRRTRLSLEDFHTVKVIGKGAFGEVRLVQKKDTGKIYAMKTLLKSEMYKKDQLAHVKAERDVLAGSDSPWVVSLYYSFQDAQYLYLIMEFLPGGDLMTMLIRWQLFTEDVTRFYMAECILAIETIHKLGFIHRDIKPDNILIDIRGHIKLSDFGLSTGFHKTHDSNYYKKLLQQDEATNGISKPGTYNANTTDTANKRQTMVVDSISLTMSNRQQIQTWRKSRRLMAYSTVGTPDYIAPEIFLYQGYGQECDWWSLGAIMYECLIGWPPFCSETPQETYRKIMNFEQTLQFPDDIHISYEAEDLIRRLLTHADQRLGRHGGADEIKSHPFFRGVDWNTIRQVEAPYIPKLSSITDTRFFPTDELENVPDSPAMAQAAKQREQMTKQGGSAPVKEDLPFIGYTYSRFDYLTRKNAL 87EBCD2C3C96EE11 tao3_yeast Cell morphogenesis protein PAG1 YIL129C TAO3 PAG1 Protein TAO3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MASRFTFPPQRDQGIGFTFPPTNKAEGSSNNNQISIDIDPSGQDVLEEINEAPLNTFPLHQSVTDAPIIDIPSPTDMSEGTSLNNQLLLRQQQQQGTGEGQALPPTFVEEQSDQNKISMLLPEQKQQRMQESAPPDITAKSVAEDYVTTLRQQMATDWKSPSEYALHILFTKFIRYAENKLNMCLQQLDMAEPPIVEILGEGVDPSFDEIIKSLGHIAKKKPKPVIDAMMFWRKTKSEAANSASEEMEKLLKEYEFEKAHPSQAHFLMNRRLSRSSSNTTSKYKHNNNTNNLPGMKRHVSSSFNNKVPLIKASSSNNSATSSPSIANSQLKSLENTIEVAKEEAFLADRKSLISIYILCRVLNEIVKQASSNEEEDLSDKLEEIVFTQLKTTDPLSISTSLIKSSNWNSFAELLGSMSEKKFLSVSDRFIADLEKIPAYIPPELEPSTHLLILGMRYLKLRNYPLEKFEESADFMKSLSKFFAKTENFPVCLAYAEVTNQLLLPLAGSLTAEVNHPTWVEAMSTLLNTAKRLQADSKYWVSGFKLTVSILCASPPDLFSKQWLSLLEANASKVKSKSLNERIIFAVGLSRLVWVYLYRCPETLNNTTRTLTKLLQLYLNTRKKENWITGDFGLLNPLTDALISIGFLHPNFLMEQALIPLIRQSFNGSNLENINYEKLILTINTYKGLLVTKERPRFPEDDNRLYELNLNNITVNQVQEASSINHTEISDYFYKLFLLLDSSIGSEVWSPENQHQKQSSNAFSPFGFSFSNDNDSSKNKSLYVILFGTIIEAIPCCLSISRTIPYKSTIEILSRNAVHSEVIISSSSQNALRALASKKNPYTLITWFAKYSFDFDEKTQSSYNMSYLSSKEYNRLLILYVELLECWLEEFQSSNKEENKKETGLDGIRLLPIDAEQEESNETEKLEWKNTVTVIEEVEGNGLFFLCSHDAKIRRLGIQILRIIFKFDEAMMEKTEKLSNGHSRSSSHFAADRGTRLIDLLNECNTTTLINPHKATLSAVEKTRFSRLNSKYKRGLLIKLAESEYGVDAALWQRAFPKLLALVFKTCPMAMALCRSIVCIRLVQVHEIILRVANDVDFKLKNVLPETIVNQWKLYLIAACTSLTSTFDQKLHIPSNIPQHGRKKSQQIFTVQHQKIKSAKSIFKMVLPLLNAKYIMIRDAIITGLSSMNINIFKAYVEAIDVFLVAWKEGSSNNQIRVEMFHILTILSPYLKSDMIFNDEWILRKLSEFLQKTKQFLEKDSVQISYEYQSLRSYFAGLILSYYMAVREHPLIDELFPFQARASCFNFLKEWCGYGEYEPISEERYAIMIKNTESGRDRTAITTGIEFQKNRLQMIVLETMVVLCSDPITQTLDDDLELPIVISFDTEDLLAWIEALFDSDNTTVKNLGVRALENLLDKNRENFKLFRDVAFQCVSHHSHPSVAVLYYTTLCKSVLKLDNLVLDEDELVSLGLYGLVADKEDTRTFAVDLLSAVETKLHNSSYTKVFKERLANSSKTVYKSTAKEISSIFAELLSQDLCLRIFSSLVRILDLFPFEIKRDLLVLMVPWVNKFTLKSLEELDTFMVLNNLFYITIDLNDSLPNEVEQLWISLGKGNSFQNIHVSLEYIINSSMNHCNPLFVQYARDIVLYLANIPGGIGLLDTLLNNLEPKYMVPLAKHTFNEPMNNNKYSFLGNIWERLNYNGKRIIFSKAQLSIIFLVNLLTNLSESVKAKIPLLLHMSICLLDHYVPLIHESACKIASTLIFGLAPSHEKSEETVKLLRNKHALWSYDNLMKKGARSPKTMDLLIRNIISIFSDLDEFQVTWQRIALKWATTCSVRHIACRSFQIFRSLLTFLDQEMLRDMLHRLSNTISDGNVDIQGFAMQILMTLNAIMAELDPTNLISFPQLFWSITACLSSIHEQEFIEVLSCLSKFISKIDLDSPDTVQCLVAIFPSNWEGRFDGLQQIVMTGLRSANSLEITWKFLDKLNLLKDSRIIANTESRLLFALIANLPRFLNAMDRKDFTGIQVAADSLIELANAYKQPSLSRLIDSLAKNKFRSKKDFMSQVVSFISRNYFPSYSAQTLVFLLGLLFNKIGWIRVQTLEILKYVFPLIDLRRPEFIGVGADLISPLLRLLFTEYEAKALEVLDCVPNVSGSKMDKDVLRITMGNKDVKDGDNATTTLFGLPEDSGWSVPMPTMTAATTRHNVHAVFMTCGTGKSDEVSAHGSDDMDAVIEFHADGDYELGRMDTIVEFHADGDYDLGRMDTNDSISVAEEKDASLSHMWAELDNLDSFFTKDTNVPNISSKMGMGIPHGRSDSIETTRTDQTFSFESAPQLYDKKVSVILNRSLSRTPSNVSFKTHLADSFAVKINRNGKPRI 08958191BE969C1B sog2_yeast Leucine-rich repeat-containing protein SOG2 SOG2 YOR353C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVATSSKRTLDPKEEHLPADKTSTNSSNTIISELATQEKSSSSGTTLKLIALNIKSISDEDVGYIQNVERLSLRKNHLTSLPASFKRLSRLQYLDLHNNNFKEIPYILTQCPQLEILDLSSNEIEALPDEISSFWQDNIRVLSLKDNNVTSIRNLKSITKLNKLSILDLEDNKIPKEELDQVQSYTPFHTGIPKEEYWAIAISRYLKDHPNLPTPEPKISRAAKRMGFINTNLSNGAMNENNIISLAPSANTTISASTAMVSSNQTSATSFSGTVNAESEQSGAVNGTELYNHTKYNDYFKRLSILPEESMSNGHQKISHAELVVSCRKLLFSFTECQQAIRKIASFCKEKAVAVNVVSLLYSVRSHTDNLVEVLQQTENEDESHDQALIKLCLTIITNFKQIITLLRKNFEIFFKEDDLCFIRMFYMTLMCAYMEMYNAWSFIKEDDQVSGSASKAPKKHSFSRHETSSSSITSGGGPAASTTSTHCSGNIKLLPKTRSTRTPSASALLSNSNILTGDTTAVPLLSPNLNGAHTHGPILGHQNAISNGSSQTNMNEVKTTSDTIPRQQLLQHNKSISDSKKESQAHEPKQHPVMTSSIINASNSNNVSNVNITPPPMNGGGAANSSANVVETNIDIQLYQTLSTVVKMVSVVYNQLTSEISKIAIASTMGKQILTDSLAPKIRDLTETCRQAMDLSKQLNERLNVLIPNDSNSEKYLTSLEKLKTWEIMNSFLKVIISILANTKIVMSDVPNLNELRPNLANLAKITKDVTVILDLSSYKAVSVSANSPE B0EA559AA4F66199 mob2_yeast CBK1 kinase activator protein MOB2 YFL035C YFL035C-A YFL034C-B MOB2 MPS1 binder 2 Maintenance of ploidy protein MOB2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFFNFKAFGRNSKKNKNQPLNVAQPPAMNTIYSSPHSSNSRLSLRNKHHSPKRHSQTSFPAQKSTPQSQQLTSTTPQSQQQEASERSESQQIMFLSEPFVRTALVKGSFKTIVQLPKYVDLGEWIALNVFEFFTNLNQFYGVVAEYVTPDAYPTMNAGPHTDYLWLDANNRQVSLPASQYIDLALTWINNKVNDKNLFPTKNGLPFPQQFSRDVQRIMVQMFRIFAHIYHHHFDKIVHLSLEAHWNSFFSHFISFAKEFKIIDRKEMAPLLPLIESFEKQGKIIYN CFBA15FF83BFAA33 hym1-kic1-1 HYM1 binding proteins by Y2H. 573326 N/A 573329 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573330 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6A. cbk1-cbk1 CBK1 binding proteins by Y2H. 573326 N/A 573331 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573331 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6A. tao3-kic1-1 TAO3 binding proteins by Y2H. 573326 N/A 573329 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573332 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6A. tao3-cbk1-1 TAO3 binding proteins by Y2H. 573326 N/A 573331 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 573332 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6A. tao3-cbk1-2 TAO3 binding proteins by Y2H. 573326 N/A 573332 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 573331 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6A. tao3-kic1 TAO3 binding proteins by Y2H. 573326 N/A 573329 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573332 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6A. hym1-sog2 HYM1 binding proteins by Y2H. 573326 N/A 573330 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573333 unspecified role unspecified role prey prey physical association physical association false false false Figure 6A. cbk1-cbk1-1 CBK1 binding proteins by Y2H. 573326 N/A 573331 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 573331 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6A. cbk1-kic1 CBK1 binding proteins by Y2H. 573326 N/A 573329 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573331 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6A. cbk1-cbk1-2 CBK1 binding proteins by Y2H. 573326 N/A 573331 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573331 unspecified role unspecified role prey prey physical association physical association false false false Figure 6A. cbk1-mob2 CBK1 binding proteins by Y2H. 573326 N/A 573331 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573334 unspecified role unspecified role prey prey physical association physical association false false false Figure 6A. cbk1-sog2 CBK1 binding proteins by Y2H. 573326 N/A 573331 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573333 unspecified role unspecified role prey prey physical association physical association false false false Figure 6A. hym1-kic1 HYM1 interaction with KIC1 by coimmunoprecipitation. 573327 N/A 573330 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 573329 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 6B. hym1-sog2-1 HYM1 interaction with SOG2 by Y2H. 573328 N/A 573330 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 573333 unspecified role unspecified role prey prey physical association physical association false false false