UniProt UniProt http://www.uniprot.org sakai-2003-3 Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein-protein assembly. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Sakai A., Hizume K., Sutani T., Takeyasu K., Yanagida M. Molecular weight estimation by CBB staining yanagida@kozo.biophys.kyoto-u.ac.jp EMBO J. (0261-4189) 2003 sakai-2003-1 Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein-protein assembly. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Sakai A., Hizume K., Sutani T., Takeyasu K., Yanagida M. Molecular weight estimation by CBB staining yanagida@kozo.biophys.kyoto-u.ac.jp EMBO J. (0261-4189) 2003 sakai-2003-2 Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein-protein assembly. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast pull down pull down anti tag western anti tag western blot anti tag western Sakai A., Hizume K., Sutani T., Takeyasu K., Yanagida M. Molecular weight estimation by CBB staining yanagida@kozo.biophys.kyoto-u.ac.jp EMBO J. (0261-4189) 2003 sakai-2003-4 Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein-protein assembly. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast pull down pull down western blot western blot Immuno blot Immuno blot Sakai A., Hizume K., Sutani T., Takeyasu K., Yanagida M. Molecular weight estimation by CBB staining yanagida@kozo.biophys.kyoto-u.ac.jp EMBO J. (0261-4189) 2003 smc3_schpo Structural maintenance of chromosomes protein 3 psm3 smc3 SPAC10F6.09c Cohesin complex Psm3 subunit protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MYITKIVIQGFKSYKDYTVIEPLSPHHNVIVGRNGSGKSNFFAAIRFVLSDAYTHLSREERQALLHEGPGATVMSAYVEVTFANADNRFPTGKSEVVLRRTIGLKKDEYSLDKKTVSKTEVINLLESAGFSRSNPYYIVPQGRVTSLTNAKDSERLELLKEVAGTQIYENRRAESNKIMDETIQKSEKIDELLQYIEERLRELEEEKNDLAVYHKKDNERRCLEYAIYSREHDEINSVLDALEQDRIAALERNDDDSGAFIQREERIERIKAEITELNHSLELLRVEKQQNDEDYTNIMKSKVALELQSSQLSRQIEFSKKDESSKLNILSELESKISEKENELSEILPKYNAIVSEADDLNKRIMLLKNQKQSLLDKQSRTSQFTTKKERDEWIRNQLLQINRNINSTKENSDYLKTEYDEMENELKAKLSRKKEIEISLESQGDRMSQLLANITSINERKENLTDKRKSLWREEAKLKSSIENVKDDLSRSEKALGTTMDRNTSNGIRAVKDIAERLKLEGYYGPLCELFKVDNRFKVAVEATAGNSLFHIVVDNDETATQILDVIYKENAGRVTFMPLNKLRPKAVTYPDASDALPLIQYLEFDPKFDAAIKQVFSKTIVCPSIETASQYARSHQLNGITLSGDRSDKKGALTAGYRDYRNSRLDAIKNVKTYQIKFSDLQESLEKCRSEIESFDQKITACLDDLQKAQLSLKQFERDHIPLKDELVTITGETTDLQESMHHKSRMLELVVLELHTLEQQANDLKSELSSEMDELDPKDVEALKSLSGQIENLSHEFDAIIKERAHIEARKTALEYELNTNLYLRRNPLKAEIGSDNRIDESELNSVKRSLLKYENKLQIIKSSSSGLEEQMQRINSEISDKRNELESLEELQHEVATRIEQDAKINERNAAKRSLLLARKKECNEKIKSLGVLPEEAFIKYVSTSSNAIVKKLHKINEALKDYGSVNKKAYEQFNNFTKQRDSLLARREELRRSQESISELTTVLDQRKDEAIERTFKQVAKSFSEIFVKLVPAGRGELVMNRRSELSQSIEQDISMDIDTPSQKSSIDNYTGISIRVSFNSKDDEQLNINQLSGGQKSLCALTLIFAIQRCDPAPFNILDECDANLDAQYRSAIAAMVKEMSKTSQFICTTFRPEMVKVADNFYGVMFNHKVSTVESISKEEAMAFVEG FD4D1E81A9E2E423 smc1_schpo Structural maintenance of chromosomes protein 1 psm1 smc1 SPBC29A10.04 Chromosome segregation protein smc1 Cohesin complex subunit psm1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGRLLRLEVENFKSYRGHQIIGPFEDFTSIIGPNGAGKSNLMDAISFVLGVKSSHLRSTNVKELIYRGKILQRDNTDFTDSSNPTTAYVKLMYELDNGEQREYKRAITPSGATEYKIDEEIVTFSEYCGSLQKENILVRARNFLVFQGDVETIASQSPLELSKLVEQISGSLEYKSEYDKSKDEQDKAVNLSAHSFNKKRGINAELRQYQEQKTEAERYQSQKEKRDSAQLVYLLWKLFHLEKSISSNMAEVTRLKADSIQLIERRDENTKEIEKLKEKEGSIRRNLLAFDRKVRKQEKLIASKRPELISIAEKALESKSNLRKIQRKAAEIEKDYSDQASTLQVLENQLTSLSAAEKEFLKDMQEKEQLKGLRLLPEDKEEYEGLRSEADKLNSNLLFKLQTLNRNIKVTSQSKDSLTSIVGDLESKIKSLHESVSSLDTERADLLAKINEKIESLELEKHDQQKKRLTYSELFHKTQELNEELQSCLQKILEASADRNESKQDAKKREALYALKRIYPEVKGRIIDLCTPTQKKYESAIAAALGKNFDAIVVETQAVAKECIDYIKEQRIGIMTFFPMDTIAASPVNQKFRGTHKGARLAIDVLNFESEYERVMISAVGNTLICDSMTVARDLSYNKRLNAKTVTLEGTVIHKTGLITGGSSNNRSAKHWDDHDFDLLTQTKDRLMHQIGEIEYQKSSCVITESDTVKLHSLESEISLLKDKYTVVSRSVEDKKKEIGHYESLIKEKQPHLSELEMELRNFVKSRDELQIQVEKVEEKIFSGFCKRIGISDIHTYDEIHRTFTQSFTQKQLEFTKQKSLLENRISFEKQRVSDTRLRLERMHKFIEKDQESIDNYEQNREALESEVATAEAELELLKEDFASENSKTEKILLAASEKKLVGKRLVSELTKLSGNITLLESEIDRYVSEWHAILRKCKLEDIDVPLREGSLTSIPIDDVSNSGDITMGEEPSEPVINFEKFGVEVDYDELDEELRNDGSESMASVLQEKLREYSEELDQMSPNLRAIERLETVETRLAKLDEEFAAARKAAKNAKERFNAVKQKRLQKFQAAFSHISEQIDPIYKELTKSPAFPLGGTAYLTLDDLDEPYLGGIKFHAMPPMKRFRDMDQLSGGEKTMAALALLFAIHSYQPSPFFVLDEIDAALDQTNVTKIANYIRQHASSGFQFVVISLKNQLFSKSEALVGIYRDQQENSSRTLSINLEGYVE rad21_schpo Cohesin subunit rad21 rad21 SPCC338.17c Double-strand-break repair protein rad21 SCC1 homolog protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFYSEAILSKKGPLAKVWLAAHWEKKLSKVQTLHTSIEQSVHAIVTEETAPMALRLSGQLMLGVVRIYSRKARYLLEDCTEALMRLKMSFQPGQVDMIEPATALQSLKGKDAVTQSANLTLPETITEFDLLVPDSTFDFQWSQLLRTPSRSSNTLELHSLPISSSPSFPSSQLSIEAGRNAQVESGFSLGESFAHVGNDMQFHLPISNSGAATPRSVHSDNQSQISIEVGRDAPAAAATDLSGIIGPQMTKSPASSVTHFSTPSMLPIGGTSLDDELLAPVDDLNLDLGLDDLLGDEQGANAPAIEADEQAETSSIHLPSDIMEDDSSRPAAAGVEEGQVVESATAPQQEKINPQKTVRRQRAIIDPVTELSSKQMKKQLADTSSITSPLCLNTSSIVFNATVNFTRNGKFNTSIFSSNLNPKVNELLQADFKQAILRKRKNESPEEVEPAKHQRTDTSTENQETAEVLDPEEIAAAELANITEAAIATLPQETVVQPEGEAPELGSPMGFPVTALESADDSLFDAPPVMLDEADLLGSERLDSSVSEALPSSQTAKDSLRNKWDPYTEGEKVSFQTLSAGCNREEAVQLFFDVLVLATKDVISVKQDVAIQNEITLTAKRGMLLSSL smc4_schpo Structural maintenance of chromosomes protein 4 cut3 smc4 SPBC146.03c Cell untimely torn protein 3 Chromosome segregation protein cut3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSDKGIFRTSSTPSIVDVTPDRGERPRKLVRSVLESPSQKDVASIVKIEQTPSRPFFNDFLKKRITDSLNERPNLLNKFMSAQDGTPSKSTGFNERSSQLVSEFTTTEDIENCEETTQVLPPRLVVYELRLTNFKSYAGTQIVGPFHPSFSSIVGPNGSGKSNVIDALLFVFGFRASKLRQSKASALIHKSATHPSLDSCDVEITFKEVNSDFTYVDGSELTVRRTAYKNNTSKYFVNGVESSFSAVSNLLKEKGIDLNHKRFLILQGEVESIAQMKPRAISEGDDGLLEYLEDIIGTSKYKPIIEENMQELSNSDDICAEKESRLKLVLSEKAKLEDSKNSVLSFLKDENELFMKQNQLYRTILYETRNKKTLVQNLLNSLEGKLQAHLEKFEQTERDISEKNEEVKSLREKAAKVKNDCTSEKKTRQSYEQQTVKIEEQLKFLLNKEKKLKKSIEALSFEKSEAENSLSSHDIDSQKLNSEIADLSLRLQQEELSLDDIRKSLQGKTEGISNAIEEKQKAMAPALEKINQLTSEKQILQVELDMLLNKENDLINDVESSQSSLDKLRNDAEENRNILSSKLKVLSDLKGEKKDVSKNIERKKETVHNTYRNLMSNRTKLEEMKASLSSSRSRGNVLESLQRLHESDNLNGFFGRLGDLATIDEAYDVAISTACPALNHIVVDNIETGQKCVAFLRSNNLGRASFIILKELAQKNLARIQTPENVPRLFDLLRFNDQKFAPAFYNVLQNTLVAKNLEQANRIAYGKTRWRVVTLSGQLIDKSGTMTGGGTRVKKGGMSSAITSDVSPASVETCDKQVQLEDTRYRQHLSELESLNQRFTEISERIPSAELEISKLQLDVSACDRLVAGEERRILQLKSDLKSIRNNNERKRNLQNKISNMDKEVEAININNEGLVTEIKTLQDKIMEIGGIRYRIQKSKVDDLHEQLKFVKDKLNKMSFKKKKNEQRSQSFQVELSNLTSEYDTTTESIATLKTELQSLNKYVDEHKSRLREFENALWDINSSIDELVKFIEFESKQMNSVKAERIELENQIQEQRTALSEVGNNENKYLKLMSNLKLHNLTEFCDQTTMDSTFPEYSEDELSSVDKSELVSNISVLKKKTEDREVDINVLSEYRRCNKEAEKRDSDYQSELQKRTDLKKVVTDLQSQRLDEFMYGFGIISMKLKEMYQIITMGGNAELELVDSLDPFSEGVLFSVMPPKKSWKNISNLSGGEKTLSSLALVFALHNYKPTPLYVMDEIDAALDFKNVSIVANYIKERTKNAQFIVISLRSNMFELSSRLVGIYKTANMTKSVTINNKEILTD F28CEDC322E5C622 smc2_schpo Structural maintenance of chromosomes protein 2 cut14 smc2 SPBP4H10.06c Cell untimely torn protein 14 Chromosome segregation protein cut14 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKIEELIIDGFKSYAVRTVISNWDDQFNAITGLNGSGKSNILDAICFVLGITNMSTVRAQNLQDLIYKRGQAGITRASVTIVFNNRDPASSPIGFENHPQVSVTRQIIMGGTSKYLINGHRALQQNVQNLFQSVQLNINNPNFLIMQGRITKVLNMKATEILSMIEEASGTRMFEERKEKAFRTMQRKEAKVEEINTLLREEIEPRLTKLRTEKKTFLEYQHIYNDLERLSHLCTAYDYYKLSLKVEELTVQASQKHSHIAEMESSLQTSKQEVLILKEKIKKIEDERMRQMSVSSDRTLDSQLQTVNENITRISTSIELKNTALEEEHGDLQQIRGKAKELETLLRGKRKRLDEVLSVYEKRKDEHQSISKDFKSQEELISSLTTGLSTTEGHETGYSRKLHEARDTLNDFKAEKETNRLKLEGLNKQISLTKPKKAEATKRCDQLNREIDILQNHVEKLKMSLKNTNSDITGEDVLQQKLKQLAKDRGNLLNELDALKSKLAYMEFTYTDPTPNFDRSKVKGLVAQLLTLNEENYDKQTALEITAGGRLYNLIVETEKIGAQLLQKGNLKRRVTIIPLNKITSFVASAERVGAAKKISNNKAQLALELIGYDDELLPAMQYVFGSTLVCDTPESAKKVTFHPSVKLKSVTLDGDVYDPSGTLTGGSVNKSAGPLLQIQKLNSLQLKLQVVTSEYEKLETQLKDLKTQNANFHRLEQEIQLKQHELTLLIEQRETDSSFRLLSDYQQYKDDVKDLKQRLPELDRLILQSDQAIKKIERDMQEWKHNKGSKMAELEKEFNQYKHKLDEFTPILEKSENDYNGVKLECEQLEGELQNHQQSLVQGESTTSLIKTEIAELELSLVNEEHNRKKLTELIEIESAKFSGLNKEIDSLSTSMKTFESEINNGELTIQKLNHEFDRLEREKSVAITAINHLEKENDWIDGQKQHFGKQGTIFDFHSQNMRQCREQLHNLKPRFASMRKAINPKVMDMIDGVEKKEAKLRSMIKTIHRDKKKIQDTVKSIDRFKRSALEKTWREVNSSFGEIFDELLPGNSAELQPPENKEFTDGLEIHVKIGSIWKDSLAELSGGQRSLVALALIMSLLKYKPAPMYILDEIDAALDLSHTQNIGRLIKTKFKGSQFIIVSLKEGMFTNANRLFHVRFMDGSSVVQAR psm3-psm1-3 566476 N/A 566480 unspecified role unspecified role neutral component neutral component hahis tag c-terminus tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 566481 unspecified role unspecified role neutral component neutral component c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 1B psm3-psm1-4 566476 N/A 566482 unspecified role unspecified role neutral component neutral component myc-his c-terminus tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 566480 unspecified role unspecified role neutral component neutral component c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 566481 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 2C cut14-cut3-5 566476 N/A 566483 unspecified role unspecified role neutral component neutral component c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 566484 unspecified role unspecified role neutral component neutral component ha-his c-terminus tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 5B psm3-psm1-1 566477 N/A 566481 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 566480 unspecified role unspecified role bait bait hahis tag c-terminus tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 1A psm3-psm1-2 566478 N/A 566481 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 566480 unspecified role unspecified role bait bait hahis tag c-terminus tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 1A psm3-psm1-5 566479 N/A 566480 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 566482 unspecified role unspecified role bait bait myc-his c-terminus tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 566481 unspecified role unspecified role prey prey association association false false false Fig. 2C