UniProt UniProt http://www.uniprot.org goshima-2003-1 The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Goshima G., Iwasaki O., Obuse C., Yanagida M. yanagida@kozo.biophys.kyoto-u.ac.jp EMBO J. (0261-4189) 2003 goshima-2003-2 The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Goshima G., Iwasaki O., Obuse C., Yanagida M. Chromatin pellets were obtained by centrifugation at 8000 rpm. Chromatin bound proteins were subsequently released by mung-bean nuclease treatment or washing with NaCl. yanagida@kozo.biophys.kyoto-u.ac.jp EMBO J. (0261-4189) 2003 ppe1_schpo Serine/threonine-protein phosphatase ppe1 ppe1 esp1 ppx1 SPCC1739.12 Phosphatase esp1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFDLDEWIATVRKCKYLPEHQLKRLCEMVKVILMEESNIQPVRTPVTVCGDIHGQFYDLLELFRVGGELPSTNYIFMGDFVDRGYFSLETFTLFMLLKARYPDKITLLRGNHESRQITQVYGFYDECQTKYGNANVWKYCCQVFDFLTLAAVIDNKILCVHGGLSPEVRTLDQIRILARAQEIPHEGSFCDLMWSDPEDIESWTVSPRGAGWLFGSKVTTEFSQINDLTLIARAHQLVQEGYKYHFADKNLVTVWSAPNYCYRCGNVASVMKVDESLEPEFRIFSAVADEDRTVPPSRKRSEYFI ekc1_schpo Extragenic suppressor of kinetochore protein 1 ekc1 SPCC663.01c SPCC777.16c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFWRLGQGFGFQSSSAIEAILDKPEDEINLKELLEENGVLDECKSHNPKLLEYLCKPEVLSQLIDYILEVDETEIPSADGGYEEPEHTRLSYIASEILSSDVWSICEACVENKTLMVKLWSFLDSEGPLNPLQASYFAKVNEHFLDKKTEETVAFIQSIDNFVEKILRHAETSAIMDLLLKFISMDRCNTAIGIADWLYSQGLIQSLLRLLSPYVDPDVQFTVADVIKAIIAISANSNEPGVIGPNSLSRELVSRQTITTLTDYMTDSKAPHSATSLINGVSIVIELIRKNNSDYDVTPVLQMPLDTHPPTTRDPIYLGTMLRLFAEKIPVFQKILLKPSTESDLMPTSFGKIKPLGFERFRICELYAELLHCSNMSLLSDPNGEAMVMQRDHLRDYLFRHNSCARDLVMSDEDDDDSTFSDKNSKDFKETEDMNGAEDMHGRAPQITKDNLNLTTTDSPMSEAEPVSEEEYKDVMETAKALHHGDDDAASDTSYEPLPESVIEDAKKLPVIGDFLKIEFIQNNVIPTILDHFFDYPWNNFLHNVVYDVVQQVLNAPMDKDQNYALAVDMFKQGKITEKIVYGQELNDKKVAKPSGFRAGYMGHLTIIADEVVKFVEHYSSTFDQELLNLINDEKWQNFVNKTLVETRNRDNQLLGGLEPSMVGYLEDMDEGEMLDANNLPEMQFALEQELESNSSDDDVVEVHRELSHNSSSNDEDDGNDEDPLSREMSRRLSFESANDSDQDNRDHFAQYMSQQISDNNANQFSSSDEDDDDDDEVVEWVSRGNENKYPRSNFFINGSDREDFSDSEEEDGNDSSDDDRGFAEEEYSDGLVLNHGK cenpa_schpo Histone H3-like centromeric protein cnp1 cnp1 sim2 SPBC1105.17 CENP-A homolog Silencing in the middle of the centromere protein 2 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAKKSLMAEPGDPIPRPRKKRYRPGTTALREIRKYQRSTDLLIQRLPFSRIVREISSEFVANFSTDVGLRWQSTALQCLQEAAEAFLVHLFEDTNLCAIHAKRVTIMQRDMQLARRIRGA mis12_schpo Centromere protein mis12 NMS complex subunit mis12 mis12 SPBC409.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLVELLEFTPLSFIDDVINITNQLLYKGVNGVDKAFSQTRFAKKAPQEIEEGLHKFEVLFESVVDRYYDGFEVYTLRNIFSYPPELKGYMRTFGKDVDYSITTEQDAAMDQAIQEAAEKLVVKMQLRRDLRMRLSRKREKKTEIEKHLERISFLNKVPENWQVTLPETTDFLLDQLGNLQHAVKRVVEASPTVHSREVDERITYLEKGYERLSNPIDQQKDFWSHHLSKLESTANTETANNIHKLLLSSEKDVGHTDEP ekc1-ppe1-1 567100 N/A 567102 unspecified role unspecified role prey prey N/A 567103 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 1F ppe1-ekc1-1 567100 N/A 567103 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 567102 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1F ekc1-ppe1-2 567101 N/A 567104 unspecified role unspecified role neutral component neutral component N/A 567105 unspecified role unspecified role neutral component neutral component N/A 567102 unspecified role unspecified role neutral component neutral component N/A 567103 unspecified role unspecified role neutral component neutral component c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 5B, 5C mis12-cnp1-1 567101 N/A 567105 unspecified role unspecified role neutral component neutral component N/A 567104 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 5D, 5E, 5F