IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ moraes-2003-1 Identification and characterization of proteins that selectively interact with isoforms of the mRNA binding protein AUF1 (hnRNP D). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Moraes KC., Quaresma AJ., Maehnss K., Kobarg J. Synapse - Interactions of proteins with an established role in the presynapse. Biol. Chem. (1431-6730) 2003 moraes-2003-4 Identification and characterization of proteins that selectively interact with isoforms of the mRNA binding protein AUF1 (hnRNP D). in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift predetermined predetermined participant predetermined Moraes KC., Quaresma AJ., Maehnss K., Kobarg J. Synapse - Interactions of proteins with an established role in the presynapse. Biol. Chem. (1431-6730) 2003 moraes-2003-2 Identification and characterization of proteins that selectively interact with isoforms of the mRNA binding protein AUF1 (hnRNP D). in vitro In vitro pull down pull down predetermined predetermined participant predetermined Moraes KC., Quaresma AJ., Maehnss K., Kobarg J. Synapse - Interactions of proteins with an established role in the presynapse. Biol. Chem. (1431-6730) 2003 moraes-2003-6 Identification and characterization of proteins that selectively interact with isoforms of the mRNA binding protein AUF1 (hnRNP D). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Moraes KC., Quaresma AJ., Maehnss K., Kobarg J. Synapse - Interactions of proteins with an established role in the presynapse. a human fetal brain cDNA library was used. Biol. Chem. (1431-6730) 2003 moraes-2003-3 Identification and characterization of proteins that selectively interact with isoforms of the mRNA binding protein AUF1 (hnRNP D). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Moraes KC., Quaresma AJ., Maehnss K., Kobarg J. Synapse - Interactions of proteins with an established role in the presynapse. Biol. Chem. (1431-6730) 2003 q14103-4 Heterogeneous nuclear ribonucleoprotein D0 p37 HNRNPD AUF1 HNRPD AU-rich element RNA-binding protein 1 protein protein human Homo sapiens Human MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGSAESEGAKIDASKNEEDEGKMFIGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGDQQSGYGKVSRRGGHQNSYKPY Contains a omega-N-methylarginine at position 263. Contains a N6-acetyllysine at position 273. hnrpq_human Heterogeneous nuclear ribonucleoprotein Q Synaptotagmin-binding, cytoplasmic RNA-interacting protein Glycine- and tyrosine-rich RNA-binding protein NS1-associated protein 1 SYNCRIP HNRPQ NSAP1 protein protein human Homo sapiens Human MATEHVNGNGTEEPMDTTSAVIHSENFQTLLDAGLPQKVAEKLDEIYVAGLVAHSDLDERAIEALKEFNEDGALAVLQQFKDSDLSHVQNKSAFLCGVMKTYRQREKQGTKVADSSKGPDEAKIKALLERTGYTLDVTTGQRKYGGPPPDSVYSGQQPSVGTEIFVGKIPRDLFEDELVPLFEKAGPIWDLRLMMDPLTGLNRGYAFVTFCTKEAAQEAVKLYNNHEIRSGKHIGVCISVANNRLFVGSIPKSKTKEQILEEFSKVTEGLTDVILYHQPDDKKKNRGFCFLEYEDHKTAAQARRRLMSGKVKVWGNVGTVEWADPIEDPDPEVMAKVKVLFVRNLANTVTEEILEKAFSQFGKLERVKKLKDYAFIHFDERDGAVKAMEEMNGKDLEGENIEIVFAKPPDQKRKERKAQRQAAKNQMYDDYYYYGPPHMPPPTRGRGRGGRGGYGYPPDYYGYEDYYDYYGYDYHNYRGGYEDPYYGYEDFQVGARGRGGRGARGAAPSRGRGAAPPRGRAGYSQRGGPGSARGVRGARGGAQQQRGRGVRGARGGRGGNVGGKRKADGYNQPDSKRRQTNNQNWGSQPIAQQPLQGGDHSGNYGYKSENQEFYQDTFGQQWK ybox1_human Y-box-binding protein 1 YBX1 YB1 NSEP1 Y-box transcription factor CCAAT-binding transcription factor I subunit A Enhancer factor I subunit A DNA-binding protein B Nuclease-sensitive element-binding protein 1 protein protein human Homo sapiens Human MSSEAETQQPPAAPPAAPALSAADTKPGTTGSGAGSGGPGGLTSAAPAGGDKKVIATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPGGVPVQGSKYAADRNHYRRYPRRRGPPRNYQQNYQNSESGEKNEGSESAPEGQAQQRRPYRRRRFPPYYMRRPYGRRPQYSNPPVQGEVMEGADNQGAGEQGRPVRQNMYRGYRPRFRRGPPRQRQPREDGNEEDKENQGDETQGQQPPQRRYRRNFNYRRRRPENPKPQDGKETKAADPPAENSSAPEAEQGGAE ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS hnrpd_human Heterogeneous nuclear ribonucleoprotein D0 HNRNPD AUF1 HNRPD AU-rich element RNA-binding protein 1 protein protein human Homo sapiens Human MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGSAESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGGPSQNWNQGYSNYWNQGYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY if2b2_human Insulin-like growth factor 2 mRNA-binding protein 2 IGF-II mRNA-binding protein 2 Hepatocellular carcinoma autoantigen p62 VICKZ family member 2 IGF2BP2 IMP2 VICKZ2 protein protein human Homo sapiens Human MMNKLYIGNLSPAVTADDLRQLFGDRKLPLAGQVLLKSGYAFVDYPDQNWAIRAIETLSGKVELHGKIMEVDYSVSKKLRSRKIQIRNIPPHLQWEVLDGLLAQYGTVENVEQVNTDTETAVVNVTYATREEAKIAMEKLSGHQFENYSFKISYIPDEEVSSPSPPQRAQRGDHSSREQGHAPGGTSQARQIDFPLRILVPTQFVGAIIGKEGLTIKNITKQTQSRVDIHRKENSGAAEKPVTIHATPEGTSEACRMILEIMQKEADETKLAEEIPLKILAHNGLVGRLIGKEGRNLKKIEHETGTKITISSLQDLSIYNPERTITVKGTVEACASAEIEIMKKLREAFENDMLAVNQQANLIPGLNLSALGIFSTGLSVLSPPAGPRGAPPAAPYHPFTTHSGYFSSLYPHHQFGPFPHHHSYPEQEIVNLFIPTQAVGAIIGKKGAHIKQLARFAGASIKIAPAEGPDVSERMVIITGPPEAQFKAQGRIFGKLKEENFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVNELQNLTSAEVIVPRDQTPDENEEVIVRIIGHFFASQTAQRKIREIVQQVKQQEQKYPQGVASQRSK q14103-3 Heterogeneous nuclear ribonucleoprotein D0 p40 Dx7 HNRNPD AUF1 HNRPD AU-rich element RNA-binding protein 1 protein protein human Homo sapiens Human MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGSAESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGDQQSGYGKVSRRGGHQNSYKPY Contains a omega-N-methylarginine at position 282. Contains a N6-acetyllysine at position 292. 38mer rna ribonucleic acid RNA rna human Homo sapiens Human GUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUAG o60506-1 Heterogeneous nuclear ribonucleoprotein Q hnRNP Q3 SYNCRIP HNRPQ NSAP1 Glycine- and tyrosine-rich RNA-binding protein NS1-associated protein 1 Synaptotagmin-binding, cytoplasmic RNA-interacting protein protein protein human Homo sapiens Human MATEHVNGNGTEEPMDTTSAVIHSENFQTLLDAGLPQKVAEKLDEIYVAGLVAHSDLDERAIEALKEFNEDGALAVLQQFKDSDLSHVQNKSAFLCGVMKTYRQREKQGTKVADSSKGPDEAKIKALLERTGYTLDVTTGQRKYGGPPPDSVYSGQQPSVGTEIFVGKIPRDLFEDELVPLFEKAGPIWDLRLMMDPLTGLNRGYAFVTFCTKEAAQEAVKLYNNHEIRSGKHIGVCISVANNRLFVGSIPKSKTKEQILEEFSKVTEGLTDVILYHQPDDKKKNRGFCFLEYEDHKTAAQARRRLMSGKVKVWGNVGTVEWADPIEDPDPEVMAKVKVLFVRNLANTVTEEILEKAFSQFGKLERVKKLKDYAFIHFDERDGAVKAMEEMNGKDLEGENIEIVFAKPPDQKRKERKAQRQAAKNQMYDDYYYYGPPHMPPPTRGRGRGGRGGYGYPPDYYGYEDYYDYYGYDYHNYRGGYEDPYYGYEDFQVGARGRGGRGARGAAPSRGRGAAPPRGRAGYSQRGGPGSARGVRGARGGAQQQRGRGVRGARGGRGGNVGGKRKADGYNQPDSKRRQTNNQNWGSQPIAQQPLQGGDHSGNYGYKSENQEFYQDTFGQQWK hnrpd-syncrip 510656 N/A 510661 unspecified role unspecified role bait bait N/A 510662 unspecified role unspecified role prey prey physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 1C NSAP-1 interacted only with AUF1p37 and p40 but not with p42 or p45. hnrpd-ybx1 510656 N/A 510661 unspecified role unspecified role bait bait N/A 510663 unspecified role unspecified role prey prey physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 1C NSEP-1 interacted only with AUF1p37 and p40 but not with p42 or p45. hnrpd-ube2i 510656 N/A 510664 unspecified role unspecified role prey prey N/A 510665 unspecified role unspecified role bait bait physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 1C protein UBCE2I interacted with all four isoforms. hnrpd-igf2bp2-2 510656 N/A 510666 unspecified role unspecified role prey prey N/A 510661 unspecified role unspecified role bait bait physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 1c IMP-2 interacted only with AUF1p37 and p40 but not with p42 or p45. hnrpd-syncrip-3 510656 N/A 510662 unspecified role unspecified role prey prey N/A 510667 unspecified role unspecified role bait bait physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 1C NSAP-1 interacted only with AUF1p37 and p40 but not with p42 or p45. hnrpd-ybx1-4 510656 N/A 510663 unspecified role unspecified role prey prey N/A 510667 unspecified role unspecified role bait bait physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 1C NSEP-1 interacted only with AUF1p37 and p40 but not with p42 or p45. hnrpd-igf2bp2-3 510656 N/A 510666 unspecified role unspecified role prey prey N/A 510667 unspecified role unspecified role bait bait physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1. p42 IsoformId:Q14103-2. p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 1c IMP-2 interacted only with AUF1p37 and p40 but not with p42 or p45. hnrpd-hnrpd 510656 N/A 510667 unspecified role unspecified role prey prey N/A 510661 unspecified role unspecified role bait bait physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 1B hnrpd-hnrpd-1 510656 N/A 510661 unspecified role unspecified role prey prey N/A 510661 unspecified role unspecified role bait bait physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 1B igf2bp2-38mer 510657 N/A 510666 unspecified role unspecified role neutral component neutral component n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) N/A 510668 unspecified role unspecified role neutral component neutral component 5 primer radiolabelled radiolabel radiolabeled radiolabelled certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false 4D (B-E) Binding of the interacting proteins both in the absence (nil) and presence of 52 nM 6xHis-AUF1p37. syncrip-38mer-1 510657 N/A 510662 unspecified role unspecified role neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 510668 unspecified role unspecified role neutral component neutral component 5 primer radiolabelled radiolabel radiolabeled radiolabelled certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4B (B-E) Binding of the interacting proteins both in the absence (nil) and presence of 52 nM 6xHis-AUF1p37. ybx1-38mer-1 510657 N/A 510663 unspecified role unspecified role neutral component neutral component n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) N/A 510668 unspecified role unspecified role neutral component neutral component 5 primer radiolabelled radiolabel radiolabeled radiolabelled certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false 4C (B-E) Binding of the interacting proteins both in the absence (nil) and presence of 52 nM 6xHis-AUF1p37. ube2i-38mer 510657 N/A 510664 unspecified role unspecified role neutral component neutral component n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) N/A 510668 unspecified role unspecified role neutral component neutral component 5 primer radiolabelled radiolabel radiolabeled radiolabelled certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false 4E (B-E) Binding of the interacting proteins both in the absence (nil) and presence of 52 nM 6xHis-AUF1p37. hnrpd-38mer 510657 N/A 510668 unspecified role unspecified role neutral component neutral component 5 primer radiolabelled radiolabel radiolabeled radiolabelled certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 510661 unspecified role unspecified role neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1. p42 IsoformId:Q14103-2. p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.AUF1 p37 isoform was used in the experiment. 4A hnrpd-ybx1-1 510658 N/A 510661 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) N/A 510663 unspecified role unspecified role bait bait n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 2 NSEP-1 interact with AUF1p37 invitro. hnrpd-igf2bp2-1 510658 N/A 510661 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) N/A 510666 unspecified role unspecified role bait bait n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 2 IMP-2 interact with AUF1p37 invitro. hnrpd-ube2i-1 510658 N/A 510664 unspecified role unspecified role bait bait n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) N/A 510661 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 2 UBCE2I interact with AUF1p37 invitro. hnrpd-syncrip-1 510658 N/A 510669 unspecified role unspecified role bait bait n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) N/A 510661 unspecified role unspecified role prey prey ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 2 NSAP-1 interact with AUF1p37 invitro. hnrpd-syncrip-6 510659 N/A 510661 unspecified role unspecified role bait bait N/A 510662 unspecified role unspecified role prey prey n terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 hnrpd-ybx1-7 510659 N/A 510667 unspecified role unspecified role bait bait N/A 510663 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false table 1 hnrpd-igf2bp2-6 510659 N/A 510666 unspecified role unspecified role prey prey N/A 510661 unspecified role unspecified role bait bait physical association physical association false false false table 1 hnrpd-ube2i-2 510659 N/A 510665 unspecified role unspecified role bait bait N/A 510664 unspecified role unspecified role prey prey physical association physical association false false false table 1 hnrpd-igf2bp2 510660 N/A 510661 unspecified role unspecified role bait bait ecobb Escherichia coli (strain B / BL21) N/A 510666 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 3 hnrpd-syncrip-2 510660 N/A 510662 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 510661 unspecified role unspecified role bait bait physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 3 These results show that at least two domains of NSAP1 are required for the interaction with AUF1p37 and that domain 3 alone is not sufficient for the interaction. hnrpd-ybx1-3 510660 N/A 510663 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 510661 unspecified role unspecified role bait bait physical association physical association false false false AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. p45 IsoformId:Q14103-1.p42 IsoformId:Q14103-2.p40 IsoformId:Q14103-3. p37 IsoformId:Q14103-4.four isoforms were used in the experiment. 3