UniProt UniProt http://www.uniprot.org wicky-2003-4 Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wicky S., Frischmuth S., Singer-Kruger B. singer-krueger@po.uni-stuttgart.de FEBS Lett. (0014-5793) 2003 Only protein-protein interactions imex curation wicky-2003-1 Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wicky S., Frischmuth S., Singer-Kruger B. singer-krueger@po.uni-stuttgart.de FEBS Lett. (0014-5793) 2003 Only protein-protein interactions imex curation wicky-2003-2 Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wicky S., Frischmuth S., Singer-Kruger B. singer-krueger@po.uni-stuttgart.de FEBS Lett. (0014-5793) 2003 Only protein-protein interactions imex curation wicky-2003-3 Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Wicky S., Frischmuth S., Singer-Kruger B. singer-krueger@po.uni-stuttgart.de FEBS Lett. (0014-5793) 2003 Only protein-protein interactions imex curation inp52_yeast Polyphosphatidylinositol phosphatase INP52 YNL106C N2160 INP52 SJL2 Synaptojanin-like protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKILLSKQQTRKIAIVSETHGLVFRPINSKNSRRSTCAVELVPKAELNGNGFRRLSNHEIYGFIGLIEIEGLMFIATITGKSKVAQPIPNKTVNKIYAVDFFCLNNSKWDFMDIDSSGYPIVTNDGDFAISSPPSISTHSSRSSLRSSSSRSLNAQEQAPKHPCHELRKLLSNGSFYYSTDFDLTCTLQKRGFTEHSLSFDDFDREFMWNSFLMDEIITYRDRLDVTAKELLDQRGFLTTVIRGFAETIFSYINRLKVGLTIISRQSWKRAGTRFNARGIDDDGHVANFVETEMIMYSSQYCYAFTQIRGSLPIFWEQDTSLISPKIQITRSVEATQPTFDEHFIRLFKKYGPVHIINLLSTKSSEIQLSRRYKEQLKNSEKMKIGRDVFLTSFDFHRETSQDGFAAASRIIPKIRNTILDAGYFSYDVKEGRLISEQDGVFRTNCLDCLDRTNLIQQTISLAVFKLFLEDFRLVKPSSFIDDNEFVQKVNALWADNGDQISQIYTGTNALKSSYSRKGKMSFSGALSDATKSVSRMYINNFVDKGKQQNIDTLLGKLPHQQVVELYDPICEYVNERLLESEEKFTTHSNINLFVGTFNVNGNSRRADLSKWLFPIGDKFKPDVVVLGLQEVIELTAGSILNADYTKSSFWETMVTDCLNQYEEKYLLLRVEQMSSLLILFFARSDRAYNIKEVGGSTKKTGFGGITGNKGAVAIRFDYGATSFCFVNTHLSAGASNIDERRNDYNNIYRNITFPRSKTIPHHDSLFWLGDLNYRITLTNDEVRRELRAQKDGYIDRLLQYDQLTQEINEGVVFQGFKEPTLQFRPTYKYDYGTDNYDTSEKARTPSWTDRIIYKGENLHPLAYSDAPLKISDHKPVYAAYRANVKFVDEKEKLNLVEKLYAEYKNTHPEALTTGPDELSHARMEKQKESIPLDATVQSAGIKLIDLDDTSSCVSPLLSGPSPQPSVVGPGGLSNVSPDKSKLNVLPPPPPTSRHNKEPSSKLLSPTKEISIVSVSPRKGESNLPALERHSTPKPLPPVPALSLSKPVSLQKSSSELQHAKETIDNGKIVPRPCPPIRRKSSTAPDEISTSTKNSGVSTTEDPEPAKASTKPEKPPVVKKPHYLSVAANKLNTSQEHSIKVSPSNSKSEEELPCKKKSKPKVPAKNPELEKLSVHPLKPCDPN 97B279870DFE6F65 bsp1_yeast Protein BSP1 BSP1 YPR171W Binding of synaptojanin polyphosphoinositide phosphatase domain protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTKYERDPELVNFLSKVEDLNSKRYSNIPSSKPAGEALSPVRSHNSGEYRRADMMTGKNVEGCDNLAYRSAYNYEMTFSPKKTHYSLSELNLERITPRPDLEGSASQKEKKFLISEEDYLLLQKLKASQTYNDSNADKNLPSFEKGPRMPSRGRPRPREKEIITIQYDFELPGRADIPSSSSSSSPPPLPTRRDHIKITDGNEEKPLLPTRPNKAEVTESPSSRSIKPDAVVPERVKPAPPVSRSTKPASFLSSLEDNKLTKAKSYNSEMETPKTTVKSSHIDYLDSIQLKPTTLSPTMKNKPKPTPPSPPAKRIPRSESFIKSMLNSNLTTTSKPSLPEKPQKLRNANLAAHKTKPSIPPKKVELNIVLPELRPVETSPTKQNFENSIDLPKLRSSNRNIKKEEEDSIPEAIKGIQNLKKTKQQKPAIPQKKSFLTNNSKNTTLKNGDDINKLNDEIEALSLRNNLKKRPPTAPQRKISLPEALRKVELMKKSKTEPVLESSNELSINAKLDAIIASRNLRASNTLPELSGVNTNIATSDKYTTSRDETVKETKPLVHPNKNRTRGPRRKLPTRV inp53_yeast Polyphosphatidylinositol phosphatase INP53 Synaptojanin-like protein 3 Suppressor of PMA1 protein 2 INP53 SJL3 SOP2 YOR3231w YOR109W protein protein yeast Saccharomyces cerevisiae Baker's yeast MIIFVSEEPERRLAIVSNLYALVLKPVGKKPSDKPLCAIELLQKNDLKKYGFKRLTSHEIFGVIGLIEVNGLLFVGAITGKSKVAQPCPGETVNKIFAVDFFCLNDNSWDFIEIDSSGYPVLPETASTEYQDALPKHPCYELKKLLSNGSFYYSSDFDLTSTLQHRGYGQHSLSTDTYEEEYMWNSFLMQEMITYRDHLDTNLKQILDDEGFLTTVIRGFAETFVSYVKKLKVALTIISKQSWKRAGTRFNARGVDDEANVANFVETEFIMYSSQYCYAFTQIRGSIPVFWEQGTSLINPRVQITRSFEATQPVFDKHIMKSVEKYGPVHVVNLLSTKSSEIELSKRYKEHLTHSKKLNFNKDIFLTEFDFHKETSQEGFSGVRKLIPLILDSLLSSGYYSYDVREKKNISEQHGIFRTNCLDCLDRTNLAQQIISLAAFRTFLEDFRLISSNSFIDDDDFVSKHNTLWADHGDQISQIYTGTNALKSSFSRKGKMSLAGALSDATKSVSRIYINNFMDKEKQQNIDTLLGRLPYQKAVQLYDPVNEYVSTKLQSMSDKFTSTSNINLLIGSFNVNGATKKVDLSKWLFPIGEKFKPDIVVLGLQEVIELSAGSILNADYSKSSFWENLVGDCLNQYDDKYLLLRVEQMTSLLILFFVKADKAKYVKQVEGATKKTGFRGMAGNKGAVSIRFEYGATSFCFVNSHLAAGATNVEERRSDYESIVRGITFTRTKMIPHHDSIFWLGDMNYRINLPNEDVRRELLNQEEGYIDKLLHFDQLTLGINSGSVFEGFKEPTLKFRPTYKYDPGTGTYDSSEKERTPSWTDRIIYKGENLLPLSYSDAPIMISDHRPVYAAYRAKITFVDDKERLSLKKRLFTEYKQEHPEEPGSLISDLLSLDLDNKSTDGFKSSSESSLLDIDPIMAQPTASSVASSSPVSSASASLQPVRTQNSSQSRTPIKKPVLRPPPPPAHKSVSAPAPSTSKEKSPTPQTSTASLSSVTKNIQENKPLAQNRRIPPPGFSQNILTPKSTSNLASPMSSKVDLYNSASESTRSAQDARQQTPTAFAASRDVNGQPEALLGDENPIEPEEKAKLNHMTLDSWQPLTPK sjl2-bsp1-3 572006 N/A 572010 unspecified role unspecified role bait bait region SAC1 domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 572011 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1C The full length SAC1 domain is required for the interaction since deleting the highly conserved CX5(T/S) motif impairs the interaction. sjl3-bsp 572006 N/A 572012 unspecified role unspecified role bait bait region SAC1 domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 572011 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1C sjl2-bsp1 572007 N/A 572011 unspecified role unspecified role prey prey N/A 572010 unspecified role unspecified role bait bait region SAC1 domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false No figure sjl2-bsp1-1 572008 N/A 572011 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 572010 unspecified role unspecified role bait bait region SAC1 domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 1A sjl2-bsp1-2 572009 N/A 572011 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false in vitro In vitro N/A 572010 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 1B