IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ seol-2001-2 Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Seol JH., Shevchenko A., Shevchenko A., Deshaies RJ. Not all the protein bands observed on SDS PAGE gel were isolated for MS analysis. Yeast strains expressing epitope tagged proteins were used in this study. Antibody affinity column chromatography was used in this study. Figure 1 (a). Table 1. deshaies@its.caltech.edu Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation seol-2001-1 Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Seol JH., Shevchenko A., Shevchenko A., Deshaies RJ. Not all the protein bands observed on SDS PAGE gel were isolated for MS analysis. Yeast strains expressing epitope tagged proteins were used in this study. Antibody affinity column chromatography was used in this study. Figure 1 (a). Table 1. deshaies@its.caltech.edu Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation seol-2001-3 Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Seol JH., Shevchenko A., Shevchenko A., Deshaies RJ. Not all the protein bands observed on SDS PAGE gel were isolated for MS analysis. Yeast strains expressing epitope tagged proteins were used in this study. Antibody affinity column chromatography was used in this study. Figure 1 (a). Table 1. deshaies@its.caltech.edu Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation skp1_yeast Suppressor of kinetochore protein 1 SKP1 CBF3D YDR328C D9798.14 Centromere DNA-binding protein complex CBF3 subunit D E3 ubiquitin ligase complex SCF subunit SKP1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTSNVVLVSGEGERFTVDKKIAERSLLLKNYLNDMHDSNLQNNSDSESDSDSETNHKSKDNNNGDDDDEDDDEIVMPVPNVRSSVLQKVIEWAEHHRDSNFPDEDDDDSRKSAPVDSWDREFLKVDQEMLYEIILAANYLNIKPLLDAGCKVVAEMIRGRSPEEIRRTFNIVNDFTPEEEAAIRRENEWAEDR 746DDE6470A69432 rav1_yeast Regulator of V-ATPase in vacuolar membrane protein 1 YJR033C J1590 Suppression of the onset of impotence protein 3 RAV1 SOI3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLNFLPGRPNATPQTACQATWQNHTIFAYCSGNNLIILTNKFTRLQTIYTQSDCTAVDINSQNGFIALSFHNRVLIYKPIHQIMQNPKWTQCCQLFHDDTPVNCLRWSSDNELAIGSDFLSFWKIKDNFGVYQPILQWNQKQPKPVYNVIISQDSQLIVSIGKYDCNAKLWKRVSIVGEQAIFNLTMLPHPKPITAMRWKKEPDQVSKNNTASHALYTLCEDKVLRIWSCFEMEKNHTVQIWGEVPLSPTQKFCVIIDNWIIRQTLSVKDSEIFDISDSDIVILGSMTGEMEVLALNNLSQDPPKPMTKKTISHKKVKKATMLNDTRYLYLPEIQPYDNVKGKLSFLVHDLQGVIRHLLIDILQLINNKTEDLSAALEHKFTGHNKSVQKLVRSSDGEALLTTSRFSENGVWYPQKLNHGVSLRLQNTIQTESPIKFAVVHELGKQVICLLENGALQAWECPTNRKEDSEQKQSYLRVETRLKEEKKIHPIVMLNTPEPKHSHERHFTALIFSDGSIKAFEVSLTRGIFEVKSDSLDIDGDDIYKISIIDPVHQTFVSNRPLISLITKKGLTRTYKAIVNYNDRHVQWIKACEINTGIMNCTCIRGSSTGKLCIVNSTGKVMSLWDLNRGVLEYEETFHNPIEDIDWTSTEYGQSIVSIGFTGYALLYTQLRYDYTNNTPSYLPIEKIDITAHTAHNIGDSVWMKNGTFVVASGNQFYIKDKSLDLTDPFTYQSIGSRKILSNDILHLSSVLNGPLPVYHPQFLIQAIYANKLQLVKELLLRLFLALRKLDFESQDVSNLDSNLGMDPLKYFIAKDRDYPVESFPDPYPCFNKTVSLALTEQLTKTTLPYLTRHQQITLITVIEAVDEVTKNENIVDYNGVRFLLGVKLFLSHKNIQKSILMRDVSWALHSDNKEILLSSIDRHITSWNRAREYRIAYWIKEQDLVKKFEDIAKYEFSKDDKRDPSRCAIFYLALKKKQILLSLWKMAIGHPEQQKMVRFISNDFTVPRWRTAALKNAFVLLSKHRYMDAAVFFLLTDSLKDCVNVLCKQVHDMDLAIGVCRVYEGDNGPVLGELLTAQMLPETIKENDRWKASFIYWKLRKQEVAIKALLTAPIDLENNSSIVDKEVCVNRSFLVEDPALLYLYNHLRNRNLKYFIGSLNVEAKIECTLILRVTDILCRMGCNYLAVSLVKNWKFIERNSIPVQKLLKSPTKDRAYSAIGAMASEPISTARMRPSLFDKFGSPSASDIESPNPKLPNSLLDDFLQPPPNSTSSNSLAQSSSSAPRSILDEFVSPSYSQHKENLTPKAPNDSVGETDNSENRKDKLSKDILDDLSSQKPQKPKKSAITKNLLDDFV ef2_yeast Elongation factor 2 EFT1 YOR133W O3317 YOR3317W EFT2 YDR385W Translation elongation factor 2 Eukaryotic elongation factor 2 Ribosomal translocase protein protein yeast Saccharomyces cerevisiae Baker's yeast MVAFTVDQMRSLMDKVTNVRNMSVIAHVDHGKSTLTDSLVQRAGIISAAKAGEARFTDTRKDEQERGITIKSTAISLYSEMSDEDVKEIKQKTDGNSFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIKPVVVINKVDRALLELQVSKEDLYQTFARTVESVNVIVSTYADEVLGDVQVYPARGTVAFGSGLHGWAFTIRQFATRYAKKFGVDKAKMMDRLWGDSFFNPKTKKWTNKDTDAEGKPLERAFNMFILDPIFRLFTAIMNFKKDEIPVLLEKLEIVLKGDEKDLEGKALLKVVMRKFLPAADALLEMIVLHLPSPVTAQAYRAEQLYEGPADDANCIAIKNCDPKADLMLYVSKMVPTSDKGRFYAFGRVFAGTVKSGQKVRIQGPNYVPGKKDDLFIKAIQRVVLMMGRFVEPIDDCPAGNIIGLVGIDQFLLKTGTLTTSETAHNMKVMKFSVSPVVQVAVEVKNANDLPKLVEGLKRLSKSDPCVLTYMSESGEHIVAGTGELHLEICLQDLEHDHAGVPLKISPPVVAYRETVESESSQTALSKSPNKHNRIYLKAEPIDEEVSLAIENGIINPRDDFKARARIMADDYGWDVTDARKIWCFGPDGNGPNLVIDQTKAVQYLHEIKDSVVAAFQWATKEGPIFGEEMRSVRVNILDVTLHADAIHRGGGQIIPTMRRATYAGFLLADPKIQEPVFLVEIQCPEQAVGGIYSVLNKKRGQVVSEEQRPGTPLFTVKAYLPVNESFGFTGELRQATGGQAFPQMVFDHWSTLGSDPLDPTSKAGEIVLAARKRHGMKEEVPGWQEYYDKL FD2F8073CB9B66AA saf1_yeast SCF-associated factor 1 YBR2017 YBR280C SAF1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEVESREKEPDAGLSPDIVQATLPFLSSDDIKNLSQTNKYYNTLLDFDHSKILWHELFHKAFGTLKTNDEPFQGRNSAEFKTCTETILREAFPTLSWQEVYQLRAYDAKFYSWGYLKHGRLGYTASSNNELVATSLNGPSPRFKYGVNTPTEVPWFNSRTTSRTSNFTPSEDPLSAIKKDGDEIIAQVSSGGFSFQILTESGNLYSSGSTFSGGLKGPGPSGSQHDYNPFREMIHNMERSYPRITSRSNGSTVNTTGTFSGRRMSGSHPSTAYEPGNATTAQHITIDSGGAPAASPGGSHSGVPRTTMPSMGPHENIYSQIEMLERSANKAVPGNNHIRRMFARNSFPLYSGRDENLGSFNDIQFVAVSSGRSHFLAMDTDNNIYSWDSTESDQGVKIEFANLPSRATNPILKIASGWNFNCCYIYKVGLVAWKERDAIQKGESFAFAKYEIVPNTNDVNGDSRIVDFACLQDNCVFFINNNGDKLWKYHNGLNQIVDLNIVGKLCKINACFASLVLFTDTHCYTLKVTNGDVDKDSLTELDINENVISVASGDYHTVALTERGHLYSWGIESQDCGCLGLGPSEKIVNELHIGNWEGQRNIRVVKPTKIELPEDYICVSVTAGGWQTGALIIKKH 358D751C612744BE cdc53_yeast Cell division control protein 53 CDC53 YDL132W D2190 Cullin-A E3 ubiquitin ligase complex SCF subunit CDC53 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSETLPRSDDLEATWNFIEPGINQILGNEKNQASTSKRVYKILSPTMYMEVYTAIYNYCVNKSRSSGHFSTDSRTGQSTILVGSEIYEKLKNYLKNYILNFKQSNSETFLQFYVKRWKRFTIGAIFLNHAFDYMNRYWVQKERSDGKRHIFDVNTLCLMTWKEVMFDPSKDVLINELLDQVTLGREGQIIQRSNISTAIKSLVALGIDPQDLKKLNLNVYIQVFEKPFLKKTQEYYTQYTNDYLEKHSVTEYIFEAHEIIKREEKAMTIYWDDHTKKPLSMALNKVLITDHIEKLENEFVVLLDARDIEKITSLYALIRRDFTLIPRMASVFENYVKKTGENEISSLLAMHKHNIMKNENANPKKLALMTAHSLSPKDYIKKLLEVHDIFSKIFNESFPDDIPLAKALDNACGAFININEFALPAGSPKSATSKTSEMLAKYSDILLKKATKPEVASDMSDEDIITIFKYLTDKDAFETHYRRLFAKRLIHGTSTSAEDEENIIQRLQAANSMEYTGKITKMFQDIRLSKILEDDFAVALKNEPDYSKAKYPDLQPFVLAENMWPFSYQEVEFKLPKELVPSHEKLKESYSQKHNGRILKWLWPLCRGELKADIGKPGRMPFNFTVTLFQMAILLLYNDADVLTLENIQEGTSLTIQHIAAAMVPFIKFKLIQQVPPGLDALVKPETQFKLSRPYKALKTNINFASGVKNDILQSLSGGGHDNHGNKLGNKRLTEDERIEKELNTERQIFLEACIVRIMKAKRNLPHTTLVNECIAQSHQRFNAKVSMVKRAIDSLIQKGYLQRGDDGESYAYLA 22BC8B35034EDDEF vata_yeast V-type proton ATPase catalytic subunit A VMA1 CLS8 TFP1 YDL185W D1286 Vacuolar proton pump subunit A protein protein yeast Saccharomyces cerevisiae Baker's yeast MAGAIENARKEIKRISLEDHAESEYGAIYSVSGPVVIAENMIGCAMYELVKVGHDNLVGEVIRIDGDKATIQVYEETAGLTVGDPVLRTGKPLSVELGPGLMETIYDGIQRPLKAIKEESQSIYIPRGIDTPALDRTIKWQFTPGKFQVGDHISGGDIYGSVFENSLISSHKILLPPRSRGTITWIAPAGEYTLDEKILEVEFDGKKSDFTLYHTWPVRVPRPVTEKLSADYPLLTGQRVLDALFPCVQGGTTCIPGAFGCGKTVISQSLSKYSNSDAIIYVGCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIRNNLNTENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNGTKHKISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGITLSDDSDHQFLLANQVVVHNCGERGNEMAEVLMEFPELYTEMSGTKEPIMKRTTLVANTSNMPVAAREASIYTGITLAEYFRDQGKNVSMIADSSSRWAEALREISGRLGEMPADQGFPAYLGAKLASFYERAGKAVALGSPDRTGSVSIVAAVSPAGGDFSDPVTTATLGITQVFWGLDKKLAQRKHFPSINTSVSYSKYTNVLNKFYDSNYPEFPVLRDRMKEILSNAEELEQVVQLVGKSALSDSDKITLDVATLIKEDFLQQNGYSTYDAFCPIWKTFDMMRAFISYHDEAQKAVANGANWSKLADSTGDVKHAVSSSKFFEPSRGEKEVHGEFEKLLSTMQERFAESTD 2A4C65D2F59426FD vatb_yeast V-type proton ATPase subunit B VMA2 Vacuolar proton pump subunit B V-ATPase 57 kDa subunit VAT2 YBR1002 YBR127C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVLSDKELFAINKKAVEQGFNVKPRLNYNTVSGVNGPLVILEKVKFPRYNEIVNLTLPDGTVRQGQVLEIRGDRAIVQVFEGTSGIDVKKTTVEFTGESLRIPVSEDMLGRIFDGSGRPIDNGPKVFAEDYLDINGSPINPYARIYPEEMISTGVSAIDTMNSIARGQKIPIFSASGLPHNEIAAQICRQAGLVRPTKDVHDGHEENFSIVFAAMGVNLETARFFKQDFEENGSLERTSLFLNLANDPTIERIITPRLALTTAEYLAYQTERHVLTILTDMSSYADALREVSAAREEVPGRRGYPGYMYTDLSTIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIFVDRQLHNKGIYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYAKYAIGKDAAAMKAVVGEEALSIEDKLSLEFLEKFEKTFITQGAYEDRTVFESLDQAWSLLRIYPKEMLNRISPKILDEFYDRARDDADEDEEDPDTRSSGKKKDASQEESLI 02A7DEC571EFF7C2 vate_yeast V-type proton ATPase subunit E VMA4 VAT5 O6241 YOR332W Vacuolar proton pump subunit E V-ATPase 27 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSAITALTPNQVNDELNKMQAFIRKEAEEKAKEIQLKADQEYEIEKTNIVRNETNNIDGNFKSKLKKAMLSQQITKSTIANKMRLKVLSAREQSLDGIFEETKEKLSGIANNRDEYKPILQSLIVEALLKLLEPKAIVKALERDVDLIESMKDDIMREYGEKAQRAPLEEIVISNDYLNKDLVSGGVVVSNASDKIEINNTLEERLKLLSEEALPAIRLELYGPSKTRKFFD 7AC5169FF7E5A39C vatd_yeast V-type proton ATPase subunit D VMA8 YEL051W SYGP-ORF11 Vacuolar proton pump subunit D protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGNREQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDDAKQKMGRVMQTAAFSLAEVSYATGENIGYQVQESVSTARFKVRARQENVSGVYLSQFESYIDPEINDFRLTGLGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPRTENTIAYINSELDELDREEFYRLKKVQEKKQNETAKLDAEMKLKRDRAEQDASEVAADEEPQGETLVADQEDDVIF FDE93E1FAF0AEC5B rav2_yeast Regulator of V-ATPase in vacuolar membrane protein 2 RAV2 YD9346.12C YDR202C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSVDLFPNDRFGAEDKYDNFKDAVKECSWLIEEIVKPQLPNIIDNFSKCLEMLESDQIFKMPVSNGIPNESNKQNDSPTVKGVITRQGQYIVDFHIVVRFPQFQRGKQVMFRMNTGLNFLLIQFSKIMTHLKNILEILNQLQVATDVSEFVSKFGVAMELLNHSLILLQNPPRDLVFPEDNNFAMKEMFQDCYSVCESTAHILGLELTLCRNELCIELRNLIKVTKKPWCEIDSKTGRSFCDQIRNQVTNERNKTLSKILSENGVQVQDSTLLNHIISSFQSEAITLPEAQELLRRGVTFDNRVVMECEKLIVSTSDPTLISISAKLNSLKASMANHQANLVASKQLSTYK yl352_yeast F-box protein YLR352W YLR352W protein protein yeast Saccharomyces cerevisiae Baker's yeast MPDLKSKTLSSQSLGAAIPPEIVYQILTYQFRDLLRNDHPGTAEKFNENLTTFVKSNLTVNKTFSHICQVLIYRYCNLTTAKRFHGLLQTLKGNRCLCNKIEVADFQELTSIGLGRSSEMNKMIKNLTNETLLEFLMLTKANLREFLACENIQDDLDDNIIKYILSPGKVLSVVDFCGCSGTTFTESFIKALDKYYPNKSIDQYRLEPIQQNYQITCLGLNDCIDLPSHVLWKILKMLPELQKLDLSHTSIDDSTLYHGIPHWKNLTHLSLATCLQVTPRAVLEFFSHHPTITDPDNTSTLEWLNVSVIAHSSSWNEVHTMFLLKKLCQHGHNKTLQYLNIGGLPLHVAPSFGEDPISESTYYYQCRDSLQFIKWNFPKLKSLSIKGNSIPISTLVEFLTPIDQDHPNCAQKLKFLNISGNSYVNKWTIQDSLLYTCSPSLVALEVSFDSWQQIEKLNDRHEIIAYRYKNPNSVIKDISTAEQVKWKCYIDSSYGRRYWLYKTDPFLNRDDLDSKSNLTRYDFEGHKIIEIINQPDFLKFAQSKIMLGCGLVPQSGIRRKLCYRDLKPPVSQFLNRKGAISLGDTPLPIITPTLPRGGWRIIHNEDDNNSHVEDSQNHVNAIPRRNSLLSRPTLRSNNGSSSANPFAINVSPASQIRDGLYWDRSVHDLRELSLQEQRIQELADEQQELRTIANFEETDDEYLHDPDLQRRRSQLHLFESSRSRSGNKTRPSLTGEHSSSASFLSFSHFNHLHKRKNYYFTHPDEFVYDPKDPLTTQRYRLHFEIVNEYQVFGCIERGMYRYYSLKA A44090288065A7BD das1_yeast F-box protein DAS1 YJL149W J0634 DAS1 DST1-delta 6-azauracil sensitivity protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPFQDYFQKKKAAFINRNNKSNADASALRDINDININFAAKSKNYVFPLTKLPDELMQEVFSHLPQPDRLQLCLVNKRLNKIATKLLYRRIYLNDSNVVKSDFMHLAINWTLLNLPSSLKEEESRDIANCKLKKLIETLQNNIHITEVIQWIRINWDLDSTLQRSILSILCNQGKSLQRLENVTDPACNDIISNGHFSRSNVSSFDMAPPNSLPEMVVPENYIPNLTKYLSQRISSRLSHMTLFIDPLKLFNYLYPLDIKLQIIDLKLHWRREFYNNDYFVKKIRPGNPLTKLSEVFDKRTLKILTIISWNDTLLKRETEMLKDFKEFENLEDLSLISIKQDVHILVDLFSSLTNLKRLKMDFLEEYVPEPTNPHIFLSILLACSKLQFIDLRYDGLIPQIINIQENKFQLNQQCNCTNCQIVFSDILKGKIFMFPEDYYIHDLQDIAAKDIFKMMKYLSLLPYSKACDAYPSVRTQPMNLTNFVTKMNRNLLEYRNSKSQLVPKIVNNPHQHSTVTSTSTAHMSEPEMIIIDDDDDDDEINAAIPPSSDDTAATISTDLELPHESLTKRDIIMCYHALIHHFKSIYVTFLKSFPHLRFLMLNDIPTIVMEENNERIFEPVFYHYDYKSNLYGWSKESNKNLENDSNNNNNNSDTIARIATVM C48B6CAB40920027 ufo1_yeast Ubiquitin ligase complex F-box protein UFO1 UFO1 YML088W protein protein yeast Saccharomyces cerevisiae Baker's yeast MERPGLVLQDLPPEILINIFSHLDEKDLFTLQELSTHFRNLIHDEELWKNLFKSRVHTTHFPTFSQSSKFSVEYIERTRGLHHWQHNKAIRTKYTIIPTRNWDQPSIERIVFDYPRVAAYNDGTITILQLQNHKRQKKFKKLIYIPCTTPQGCSTMDFNINAAVFGRFDGRVFGKLLSNKSYLTPVMEFTGRHSAGVTAICNSESWDTSREDWSVSGSENGEIIWWCENKLVKMWKVSNRVIWKLAFFKDWTLIMDDEKLYIIHQMQELHSIDIPKDLDEQPMRVRFFKMDFGSMTLVLADLNNVYTISVNPNGNFGNLRKLEMPEQICAVEIDEKTSQREQNWQFAGDDGCYISLLTTQNTLYIINIRDLSSSGLKVQCKISFDEQVYVSQVTNLIVVVALPNVLQILNAMTGELIKTVLKTEKFPEFLKVSQDKIIMGSGNVLNYLKFVSSDSKKHHHSTKGKNTVSNKWNETLNTELQYYDEDEDLRRKRQSEISRLIDAYGGDLELSGDTDEENDIQLRIALLESQEAQARNQAEAGEPVGDDEDEQLRRALEESQLIYETQTNSSANHGNNTNDEIDEDDEEFLRAIRQSRVEDERRRHLRNHTTGRRNGPLSDDNFATYGAAESSERTSTENTIGSSVGVDASNNVDEDLQLAIALSLSEIN 10CA467CF9417FBC rbx1_yeast RING-box protein HRT1 E3 ubiquitin-protein ligase complex SCF subunit HRT1 High level expression reduces Ty3 transposition protein 1 HRT1 RBX1 ROC1 Regulator of cullins protein 1 YOL133W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNEVDRMDVDEDESQNIAQSSNQSAPVETKKKRFEIKKWTAVAFWSWDIAVDNCAICRNHIMEPCIECQPKAMTDTDNECVAAWGVCNHAFHLHCINKWIKTRDACPLDNQPWQLARCGR A9C3193E48CAF881 roy1_yeast Non-SCF-type F-box protein ROY1 YMR258C YM9920.12C ROY1 Repressor of YPT52 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAFQDQDIFIVFSHASLFLNQNDLLSLSLTSKKMHDMIAIPRLYSNIHITKNPVLRTNKWFLDGGKTYVSGYRSVLKTGDKNDIFLYDRIERLLETSHLKCIKQLTIDEDLFHNREEGLQLLQRLVNEITDLDVIESLDIKDPTLFELCSAKYYRLSSLKKRVVYGETGFDGIKLWQNFKSLKWQLPESLDLQNVIIPEVGVMLMKQLNGGELEIKDEAYSSLRVFEYFDSLNLRFKNLRRLKLNHVHKQGDGSATSMRLSSRAFKDVVNLSNLKALELEFSCEVDDCECDDDFLQDITGNLVSLTSLGFIEKTFTKKGYHYMDEKWDLVVNKFILNLPNVSKDLRLLSIRHDPPLNGKGIDTVDGNLLRRKKLYEKVLPKLTSLETIIAPTVLQSITSYEMYACDLLWNGCKCAFCSKYLPLFDKYIMNHQYFSTPDARYLDIIPIVFAAYTGKSLAKRFDPQKNWDLDLLQYAPEDTTWNFHGFERIHHFASYECYFDESSFEPLATIISHFFYPYMNYLIKILPNLRQTMLSGIYFSVSPELHTYETIYD rcy1_yeast Recyclin-1 J0318 J0320 YJL204C RCY1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDDLLKVPEIVTNIASYLSTVDYLSFQQVNKRVYAIINGKNDSKYWSLKLTRMGLQQVHSNEEEEITLLDENDNQNSLRIFEIYKSFTAQNSKKIFVKFYRCYNSYARKLYNNNLANFFPTSYSNDPLKQTRILNFIKKYNFSNKNDIETFTRIETNFNILREIFINSVLKESELNYQSNNLAAVARFMKILLISNEESNAIEFFKSKADLPPSLTVLPSNDELFWAEQPREEDSGGSTVIFNSKNLDTFLNQLRDFLNEKIKLADILFKDEFPVILQFIESFIQDILLDILNNILLSYSEFLKENGKDSKANYECVPELYFTFIKKFDTELNDSVNAGANFRKVVRDLLNLYLEPFVVNYMNQTTRVFESLINSQLANYDTQVQDKQREQNAKIYNTLKDQTDASSASNNELPNDLSIITETSKTVPEADSKPSTIHQSVHSTDISNDKLDFLSSFTKIFKFSNNENQRLKQQLQLAYNLNLISNNLQNIKSLISLDLCYKILQETSEKTDQIYKFHTIESLLPLIKLRCQEIFKILITQLNKNHVKPAFEKAILLLQKYNPNEIEQIEIKFNSLSPANTQVEPLVQFTELINIGDIILQMISIFYKNELIPKKIIDKNKDFLNDVIQLKKNFETSIDDFVAEGLNIGINKLMDEISFVFKTLQLPDDYNPPPPSRNSPIRDIKPTKCAIRVVELLSNHCFLLTGATDKGTIDVYQQEIGERFFNEIVKHLKKCFISTEGAIWLICDLNYFYDFIANKLKQKNVVPYFVGLKSIGQLYIISGKDSKELGKLISDLGKFNGIFTQEEIYEFVQRRSDWVRVRKDVEKVMYGLGIRDCCIM sul2_yeast Sulfate permease 2 L9449.1 SUL2 SEL2 YLR092W High-affinity sulfate transporter 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSREGYPNFEEVEIPDFQETNNTVPDLDDLELEYDQYKNNENNDTFNDKDLESNSVAKHNAVNSSKGVKGSKIDYFNPSDVSLYDNSVSQFEETTVSLKEYYDHSIRSHLTVKGACSYLKSVFPIINWLPHYNFSWFTADLIAGITIGCVLVPQSMSYAQVATLPAQYGLYSSFIGAYSYSFFATSKDVCIGPVAVMSLQTAKVIADVTAKYPDGDSAITGPVIATTLALLCGIISAAVGFLRLGFLVELISLNAVAGFMTGSAFNILWGQVPALMGYNSLVNTRAATYKVVIETLKHLPDTKLDAVFGLIPLFLLYVWKWWCGTYGPRLNDRYNSKNPRLHKIIKWTYFYAQASRNGIIIIVFTCIGWAITRGKSKSERPISILGSVPSGLKEVGVFHVPPGLMSKLGPNLPASIIVLLLEHIAISKSFGRINDYKVVPDQELIAIGVSNLLGTFFNAYPATGSFSRSALKAKCNVRTPLSGLFSGSCVLLALYCLTGAFFYIPKATLSAVIIHAVSDLLASYQTTWNFWKMNPLDFICFIVTVLITVFASIEDGIYFAMCWSCAMLILKVAFPAGKFLGRVEVAEVTDAYVRPDSDVVSYVSENNNGISTLEDGGEDDKESSTKYVTNSSKKIETNVQTKGFDSPSSSISQPRIKYHTKWIPFDHKYTRELNPDVQILPPPDGVLVYRLSESYTYLNCSRHYNIITEEVKKVTRRGQLIRHRKKSDRPWNDPGPWEAPAFLKNLKFWKKRENDPESMENAPSTSVDVERDDRPLLKILCLDFSQVAQTDATALQSLVDLRKAINQYADRQVEFHFVGIISPWVKRGLISRGFGTLNEEYSDESIVAGHTSYHVARVPQGEENPEKYSVYTASGTNLPFFHIDIPDFAKWDI 67826955AC7C0BF5 cbf3b_yeast Centromere DNA-binding protein complex CBF3 subunit B CEP3 CBF3 YMR168C YM8520.17C CBF3B CSL1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFNRTTQLKSKHPCSVCTRRKVKCDRMIPCGNCRKRGQDSECMKSTKLITASSSKEYLPDLLLFWQNYEYWITNIGLYKTKQRDLTRTPANLDTDTEECMFWMNYLQKDQSFQLMNFAMENLGALYFGSIGDISELYLRVEQYWDRRADKNHSVDGKYWDALIWSVFTMCIYYMPVEKLAEIFSVYPLHEYLGSNKRLNWEDGMQLVMCQNFARCSLFQLKQCDFMAHPDIRLVQAYLILATTTFPYDEPLLANSLLTQCIHTFKNFHVDDFRPLLNDDPVESIAKVTLGRIFYRLCGCDYLQSGPRKPIALHTEVSSLLQHAAYLQDLPNVDVYREENSTEVLYWKIISLDRDLDQYLNKSSKPPLKTLDAIRRELDIFQYKVDSLEEDFRSNNSRFQKFIALFQISTVSWKLFKMYLIYYDTADSLLKVIHYSKVIISLIVNNFHAKSEFFNRHPMVMQTITRVVSFISFYQIFVESAAVKQLLVDLTELTANLPTIFGSKLDKLVYLTERLSKLKLLWDKVQLLDSGDSFYHPVFKILQNDIKIIELKNDEMFSLIKGLGSLVPLNKLRQESLLEEEDENNTEPSDFRTIVEEFQSEYNISDILS 2E24A0508080A09B rav1-skp1 Immunoprecipitation assay reveals Rav1 and SKP1 interact 303146 N/A 303149 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303150 unspecified role unspecified role bait bait c terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rcy1-skp1 Immunoprecipitation assay reveals Rcy1 and SKP1 interact 303146 N/A 303149 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303151 unspecified role unspecified role bait bait c terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false ybr280-skp1 Immunoprecipitation assay reveals YBR280 and SKP1 interact 303146 N/A 303149 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303152 unspecified role unspecified role bait bait c terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false ybr280-cdc53 Immunoprecipitation assay reveals YBR280 and Cdc53 interact 303146 N/A 303153 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303152 unspecified role unspecified role bait bait c terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rav1-vma1 Immunoprecipitation assay reveals Rav1 and Vma1 interact 303146 N/A 303150 unspecified role unspecified role bait bait c terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303154 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rav1-vma2 Immunoprecipitation assay reveals Rav1 and Vma2 interact 303146 N/A 303155 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303150 unspecified role unspecified role bait bait c terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rav1-vma4 Immunoprecipitation assay reveals Rav1 and Vma4 interact 303146 N/A 303150 unspecified role unspecified role bait bait c terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303156 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rav1-vma8 Immunoprecipitation assay reveals Rav1 and Vma8 interact 303146 N/A 303157 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303150 unspecified role unspecified role bait bait c terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rav2-vma2 Immunoprecipitation assay reveals Rav2 and Vma2 interact 303146 N/A 303158 unspecified role unspecified role bait bait c terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303155 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rav2-vma8 Immunoprecipitation assay reveals Rav2 and Vma8 interact 303146 N/A 303157 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303158 unspecified role unspecified role bait bait c terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false cdc53-hrt1 Affinity chromatograhy assay reveals Cdc53 interacting proteins. 303147 N/A 303159 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303160 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303161 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303162 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303153 unspecified role unspecified role bait bait 9_myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association false false false The authors note that interactions between CDC53 and Q06479 was observed in a study by Uetz et al. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature. 2000 Feb 10;403(6770):623-7. Pubmed ID 10688190. skp1-rav1 Affinity chromatograhy assay reveals SKP1 interacting proteins. 303147 N/A 303162 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303163 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303164 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303158 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303152 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303153 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303165 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303166 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303160 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303151 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303150 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303149 unspecified role unspecified role bait bait 9 myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association false false false The authors note that interactions between SKP1 and Q06479, P24814 and Q12325 were observed in a study by Uetz et al. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature. 2000 Feb 10;403(6770):623-7. Pubmed ID 10688190. rav1-rav2 Immunoprecipitation analysis reveals interaction between the RAV1, RAV2 and SKP1 proteins. 303148 N/A 303149 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303158 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303150 unspecified role unspecified role bait bait 9 myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association false false false Immunoprecipitation was carried out in the presence of 0.5% Triton-X 100 which was considered by the authors to raise the stringency of binding conditions. rav1-rav2-1 Immunoprecipitation analysis reveals interaction between the RAV1, RAV2 and SKP1 proteins. 303148 N/A 303154 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303156 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303155 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303149 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303158 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 303150 unspecified role unspecified role bait bait 9 myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association false false false Immunoprecipitation was carried out in the presence of 0.2% Triton-X 100 which was considered by the authors to reduce the stringency of binding conditions.