UniProt UniProt http://www.uniprot.org yao-2000-1 BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yao S., Neiman A., Prelich G. prelich@aecom.yu.edu Mol. Cell. Biol. (0270-7306) 2000 Only protein-protein interactions imex curation yao-2000-2 BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Yao S., Neiman A., Prelich G. Since BUR1 and BUR2 are expressed at low levels, coimmunoprecipitations were performed using extracts prepared from strains in which BUR2 and FLAG-tagged BUR1 were expressed from their promoters on a high-copy-number plasmid. prelich@aecom.yu.edu Mol. Cell. Biol. (0270-7306) 2000 Only protein-protein interactions imex curation yao-2000-3 BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down predetermined predetermined participant predetermined Yao S., Neiman A., Prelich G. prelich@aecom.yu.edu Mol. Cell. Biol. (0270-7306) 2000 Only protein-protein interactions imex curation bur1_yeast Serine/threonine-protein kinase BUR1 SGV1 BUR1 YPR161C P9584.8 Bypass UAS requirement protein 1 Suppressor of GPA1-Vall50 mutation protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDNGSPAVLPKTEFNKYKIGKVKSTPAIQRDAKTNLTYIKLRKRSSEKVYGCTVFQNHYREDEKLGQGTFGEVYKGIHLETQRQVAMKKIIVSVEKDLFPITAQREITILKRLNHKNIIKLIEMVYDHSPDITNAASSNLHKSFYMILPYMVADLSGVLHNPRINLEMCDIKNMMLQILEGLNYIHCAKFMHRDIKTANILIDHNGVLKLADFGLARLYYGCPPNLKYPGGAGSGAKYTSVVVTRWYRAPELVLGDKQYTTAVDIWGVGCVFAEFFEKKPILQGKTDIDQGHVIFKLLGTPTEEDWAVARYLPGAELTTTNYKPTLRERFGKYLSETGLDFLGQLLALDPYKRLTAMSAKHHPWFKEDPLPSEKITLPTEESHEADIKRYKEEMHQSLSQRVPTAPRGHIVEKGESPVVKNLGAIPRGPKKDDASFLPPSKNVLAKPPPSKIRELHQNPRPYHVNSGYAKTAIPPPAAPAGVNRYGPNNSSRNNRFSGNSTAPNNSRNPVNRFHPETNVSSKYNKVPLPLGPQSRYQGNSNESRYKNSPNDSRYHNPRYVNKPETNFNRQPQKYSRQESNAPINKNYNPSNGSRNMAGDHHQGSRPSHPQFPISPSQGQHQLTSKPIEKKNGSFKDERAKPDESKEFQNSDIADLY CC9034A4B10A510E bur2_yeast Protein BUR2 BUR2 Bypass UAS requirement protein 2 Chromosome stability protein 4 CST4 YLR226W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSATSSSGDVKKFQAVPKPTSNASPPPASSGFNARTLWPDLIETPENQWVFECKDIIEKIGTNGPIAVEIKKNMEKCLMYFYTLKKKLNLFDHTYTASCILFYRYWFIYGIPTAITECIHISQGILVTACKTMENNRPIEAYIKATCEFLMQNIPSLKSRTNIDKLKWEFRDKLVTNEKKILCLFGFDLNISNPKELIEEVFSGYYRFNRDHNLPENFKKAFPKILQESRNFMVQAVTQPVSLLCDGYTFIVLSLIYCGLEYKKLVDKDFRYPKNFFKDRFPIEVTPENFANIFTDYKLLEENFFNLKSNKGAKLQIDSSMIDSVIDESGDVENEVSEISDPFNYELIKSGEVKEEFLNHIETRVKDLLDKAKQESMKRKAKDPIRTPDAKKPKI B317A7B429030FDF bur1-bur2 Interaction detected by Y2H 495451 N/A 495454 unspecified role unspecified role bait bait N/A 495455 unspecified role unspecified role prey prey physical association physical association false false false 4 bur1-bur2-1 Interaction detected by CoIP 495452 N/A 495455 unspecified role unspecified role prey prey N/A 495454 unspecified role unspecified role bait bait n-terminus N-terminus FLAG tagged protein BUR1 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 5A bur1-bur2-2 Interaction detected by affinity chromatography (Ni-NTA agarose) 495453 N/A 495454 unspecified role unspecified role prey prey n-terminus N-terminus FLAG tagged protein BUR1 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 495455 unspecified role unspecified role bait bait n-terminus N-terminus HIS tagged protein BUR2 his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 5B