UniProt UniProt http://www.uniprot.org sekito-2000-1 Mitochondria-to-nuclear signaling is regulated by the subcellular localization of the transcription factors Rtg1p and Rtg3p. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sekito T., Thornton J., Butow RA. butow@swmed.edu Mol. Biol. Cell (1059-1524) 2000 sekito-2000-2 Mitochondria-to-nuclear signaling is regulated by the subcellular localization of the transcription factors Rtg1p and Rtg3p. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sekito T., Thornton J., Butow RA. butow@swmed.edu Mol. Biol. Cell (1059-1524) 2000 rtg1_yeast Retrograde regulation protein 1 RTG1 YOL067C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSIPAGTDPGSCGANFKNDRKRRDKINDRIQELLSIIPKDFFRDYYGNSGSNDTLSESTPGALGLSSKAKGTGTKDGKPNKGQILTQAVEYISHLQNQVDTQNREEVELMVKATQLAKQTGTIVNDINLENTSAEVALSRIGVGPLAATNDDSVRPPAKRLSSFEYGGYGEYGNGS rtg3_yeast Retrograde regulation protein 3 RTG3 YBL0810 YBL103C protein protein yeast Saccharomyces cerevisiae Baker's yeast MMNNNESEAENQRLLDELMNQTKVLQETLDFSLVTPTPHHNDDYKIHGSAYPGGETPAQQHEKLSYINTHNSNDNNNLMGSQARSNSQTPTASTIYEEAESQSSYLDDMFRTSQGGRPVTQNSISSIGQGPLRSSYSMAYDSPVDRAMNTPLQQQEGLKAELPHDFLFQHGTDDTMYNLTDDLSSSLSSSINSDMMTPNTYSSSFSYNPQSLGPASVSSTYSPKVRSPSSSFRAGSFLSSSFRHGSINTPRTRHTSISSNMTENIGPGSVPKILGGLTSDEKLRRKREFHNAVERRRRELIKQKIKELGQLVPPSLLNYDDLGKQIKPNKGIILDRTVEYLQYLAEILEIQARKKKALLAKIKELEEKKSSVAALSPFTNNHHASSGQNNSENSEERIIDIRSVPNALMNEQNSKAELHNWEPPLYDSVGNHNHAGTMESHPHTNIHEELKEFLSGDLIEAEDNAKLMFGDDNSNPADYLLEFGSG rtg3-rtg-1-1 565521 N/A 565523 unspecified role unspecified role prey prey N/A 565524 unspecified role unspecified role bait bait physical association physical association false false false 1 rtg3-rtg-1-2 565522 N/A 565523 unspecified role unspecified role prey prey N/A 565524 unspecified role unspecified role bait bait c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 6A Several RTG3 deletion constructs have been found to interact with RTG1: delta376-486 (most strongly), delta280-298 and delta 2-279 (weakest).