UniProt UniProt http://www.uniprot.org lutz-1998-1 The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip edman degradation edman degradation Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. RNA (1355-8382) 1998 lutz-1998-2 The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. solution sedimentati cosedimentation in solution solution sedimentati western blot western blot Immuno blot Immuno blot Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. RNA (1355-8382) 1998 lutz-1998-3 The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. RNA (1355-8382) 1998 lutz-1998-4 The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. RNA (1355-8382) 1998 snrpa_human U1 small nuclear ribonucleoprotein A SNRPA protein protein human Homo sapiens Human MAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKISFAKK sfpq_human Splicing factor, proline- and glutamine-rich SFPQ PSF Polypyrimidine tract-binding protein-associated-splicing factor DNA-binding p52/p100 complex, 100 kDa subunit 100 kDa DNA-pairing protein protein protein human Homo sapiens Human MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF snrpa-sfpq-1 194447 N/A 194451 unspecified role unspecified role bait bait N/A 194452 unspecified role unspecified role prey prey physical association physical association false false false snrpa-sfpq-2 194448 N/A 194451 unspecified role unspecified role neutral component neutral component N/A 194452 unspecified role unspecified role neutral component neutral component physical association physical association false false false snrpa-sfpq-3 194449 N/A 194451 unspecified role unspecified role bait bait N/A 194452 unspecified role unspecified role prey prey physical association physical association false false false snrpa-sfpq-4 194450 N/A 194451 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 194452 unspecified role unspecified role prey prey 35s radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false