IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ owen-1998-1 A structural explanation for the recognition of tyrosine-based endocytotic signals. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Owen DJ., Evans PR. Science (0036-8075) 1998 peptide-fyralm internalization signal peptide with the sequence FYRALM protein protein in vitro In vitro FYRALM This sequence can be found in EGFR proteins The internalization sequence motif for endocytosis is thought to be: tyrosine-X-X-(hydrophobic residue) ap2m1_rat AP-2 complex subunit mu Adaptor protein complex AP-2 subunit mu AP-2 mu chain Ap2m1 Mu2-adaptin Plasma membrane adaptor AP-2 50 kDa protein Clathrin coat assembly protein AP50 Clathrin coat-associated protein AP50 Clathrin assembly protein complex 2 mu medium chain Adaptor-related protein complex 2 subunit mu protein protein rat Rattus norvegicus (Rat) Rat MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC peptide-dyqrln internalization signal peptide with the sequence DYQRLN protein protein in vitro In vitro DYQRLN This sequence is found in TGN38 proteins The internalization sequence motif for endocytosis is thought to be: tyrosine-X-X-(hydrophobic residue) ap2m1-fyralm-1 crystal structure of AP2 mu2 adaptin with the internalization peptide FYRALM of EFGR 181344 N/A 181345 unspecified role unspecified role neutral component neutral component tyrosine binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyrosine binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyrosine binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyrosine binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyrosine binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 181346 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trp 421 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false phe 174 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false asp 176 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false lys 203 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg 423 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 181357 181350 181358 181351 181360 181352 181361 181353 181362 181354 direct interaction direct interaction false false false 2.65 working 25.0%, free 29.6% ap2m1-dyqrln-1 Crystal structure of AP2 mu2 adaptin with the internalization peptide DYQRLN of TGN38 181344 N/A 181347 unspecified role unspecified role neutral component neutral component tyrosine binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyrosine binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyrosine binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyrosine binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyrosine binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 181346 unspecified role unspecified role neutral component neutral component trp 421 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false phe 174 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false asp 176 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false lys 203 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg 423 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) 181371 181366 181372 181365 181374 181367 181375 181368 181376 181369 direct interaction direct interaction false false false 2.65 working 25.0%, free 29.6%