IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ caliebe-1997-1 The chloroplastic protein import machinery contains a Rieske-type iron-sulfur cluster and a mononuclear iron-binding protein. in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel western blot western blot Immuno blot Immuno blot Caliebe A., Grimm R., Kaiser G., Lubeck J., Soll J., Heins L. Chloroplast membranes from 12- to 14-day-old pea plants (Pisum sativum L.var. Golf)were isolated and subjected to BN PAGE (non denaturing)and tricine SDS�PAGE (denaturing)prior to Western blotting. Figure 1 (C). agsoll@bot.uni-kiel.de EMBO J. (0261-4189) 1997 caliebe-1997-2 The chloroplastic protein import machinery contains a Rieske-type iron-sulfur cluster and a mononuclear iron-binding protein. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Caliebe A., Grimm R., Kaiser G., Lubeck J., Soll J., Heins L. Chloroplast membranes from 12- to 14-day-old pea plants (Pisum sativum L.var. Golf)were isolated and subjected to BN PAGE (non denaturing)and tricine SDS�PAGE (denaturing)prior to Western blotting. Figure 1 (C). agsoll@bot.uni-kiel.de EMBO J. (0261-4189) 1997 clpc_pea Chaperone protein ClpC, chloroplastic ATP-dependent Clp protease ATP-binding subunit ClpC homolog Casein lytic proteinase C protein protein pea Pisum sativum (Garden pea) Garden pea MARVLAQSLSVPGLVAGHKDSQHKGSGKSKRSVKTMCALRTSGLRMSGFSGLRTFNHLNTMMRPGLDFHSKVSKAVSSRRARAKRFIPRAMFERFTEKAIKVIMLAQEEARRLGHNFVGTEQILLGLIGEGTGIAAKVLKSMGINLKDARVEVEKIIGRGSGFVAVEIPFTPRAKRVLELSQEEARQLGHNYIGSEHLLLGLLREGEGVAARVLENLGADPTNIRTQVIRMVGESADSVTATVGSGSSNNKTPTLEEYGTNLTKLAEEGKLDPVVGRQPQIERVTQILGRRTKNNPCLIGEPGVGKTAIAEGLAQRIANGDVPETIEGKKVITLDMGLLVAGTKYRGEFEERLKKLMEEIKQSDDIILFIDEVHTLIGAGAAEGAIDAANILKPALARGELQCIGATTLDEYRKHIEKDPDLERRFQPVKVPEPTVDETIQILKGLRERYEIHHKLRYTDEALIAAAQLSYQYISDRFLPDKAIDLVDEAGSRVRLQHAQLPEEAKELDKEVRKIVKEKEEYVRNQDFEKAGELRDKEMDLKAQISALIEKGKEMSKAETETADEGPIVTEVDIQHIVSSWTGIPVDKVSADESDRLLKMEDTLHKRIIGQDEAVQAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGSPPGYVGYTEGGQLTEAVRRRPYTVVLFDEIEKAHPDVFNMMLQILEDGRLTDSKGRTVDFKNTLLIMTSNVGSSVIEKGGRRIGFDLDYDEKDSSYNRIKSLVTEELKQYFRPEFLNRLDEMIVFRQLTKLEVKEIADIMLKEVFQRLKTKEIELQVTERFRDRVVDEGYNPSYGARPLRRAIMRLLEDSMAEKMLAREIKEGDSVIVDVDSDGKVIVLNGSSGTPESLPEALSI tic55_pea Protein TIC 55, chloroplastic TIC55 Rieske iron-sulfur protein TIC55 Translocon at the inner envelope membrane of chloroplasts 55 protein protein pea Pisum sativum (Garden pea) Garden pea MALALASANSFLLPTKTHFALHVSPPPSKKTLLCTNPSSNFSFNKALSSRRRKQAWCVAAAADVKDATLLDGEEDQKVLVGPSSEQERKGEREVADYDWTEEWYPLYLTKNVPHDAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREDAQALGFEVTERTDRGFAGWWGREKDGSKPNFLRFEAPCVLQNNREIVDKNGEINHFSGLFLCRPTGQGKSMLIVRFGATKRSPLIKLFPEWYFHQNASKVFEQDMGFLSSQNEILLKEKVPTKELYLNLKSSDTWVAEYRKWMDKVGHGMPYHFGHSTISLPEEPAVVEHAPAGLVAGLSASSPAKGGIGTMHAPNLANRYFRHVIHCKGCSSAIKAFQIWKNVLSGVVVALAALAILVSGRQWKVLLLASASLCSVGVYACSTAIAMNTTNFIRVHRRL ti110_pea Protein TIC110, chloroplastic TIC110 IEP110 Chloroplast inner envelope protein, 110 kDa IAP100 Translocon at the inner envelope membrane of chloroplasts 110 protein protein pea Pisum sativum (Garden pea) Garden pea MNPSTLKPSHTHPSLLLPAPSPLRTQRRRFRVSLPRCSSDTNNPASSSSPPQRPPKELNGIEILVDKLSSPARLATSAVIVAGAVAAGYGLGSRFGGSRNAALGGAVALGAAGGAAAYALNAAAPQVAAVNLHNYVAGFDDPSILTREDIEVIANKYGVSKQDEAFKAEICDIYSEFVSSVIPPGGEELKGDEVDKIVNFKSSLGLDDPDAAAVHMEIGRKLFRQRLEVGDREGGVEQRRAFQKLIYVSNIVFGDASSFLLPWKRVFKVTESQVEVAIRDNAQRLYASKLKSVGRDFDLGKLVTLKETQSLCCLSDELAENLFREHARKLVEENISVALGILKSRTRAVPGVSQVVEEIEKVLAFNDLLISFKNHSDIDRLARGVGPVSLVGGEYDADRKIEDLKLLYRAYVSDALSSGRMEDNKFAALNQLKNIFGLGKREAEAILLDITRKVYRKRLGQTVSSGELEMADSKAAFLQNLCDELHFDPQKASELHEEIYRQKLQQCVADGELTDENVAALLKLRVMLCVPQQTVEAAHAEICGNLFEKIVKDAIASGVDGYDDETKKSVRKAAHGLRLTKETALSIASKAVRKMFITYVKRSRSAKGNGESAKELKKLIAFNTLVVTKLVEDIKGESPDVKIEEPKIEEPEEIRESEEYEMRITSDTQENKTGQRACRKDGKAWSDRITLKDDLPEKDRADLYKTFLTYCLTGDVVRIPFGVEIKKKKDDTEYIYLNQLGGILGLTGKVIMDVHRGLAEQAFRKQAEVLLADGQLTKARVEQLGKMQKEIGLSQEYAQKIIKNITTTKMAAAIETAVTQGKLNMKQIRELKESNVDLDSMVSVSLRETIFKKTVGDIFSSGTGEFDEEEVYEKIPLDLNINKEKARGVVCELAQNRLSNSLIQAVALLRQRNHKGVVFSLNNLLACDKAVPSQTLSWEVSEELSDLYTIYLKSDPSPEKLSRLQYLLGINDSTAAALRDSEDSLLETAEEEKFVF This sequence has been withdrawn from Uniprot. E36649FF7A0C2680 tc159_arath Translocase of chloroplast 159, chloroplastic T14P8.24 At4g02510 TOC159 PPI2 TOC160 TOC86 Translocase of chloroplast 160, chloroplastic Translocase of chloroplast 86, chloroplastic 159 kDa chloroplast outer envelope protein Plastid protein import 2 T10P11.19 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDSKSVTPEPTNPFYASSGQSGKTYASVVAAAAAAAADKEDGGAVSSAKELDSSSEAVSGNSDKVGADDLSDSEKEKPNLVGDGKVSDEVDGSLKEDSTTPEATPKPEVVSGETIGVDDVSSLSPKPEAVSDGVGVVEENKKVKEDVEDIKDDGESKIENGSVDVDVKQASTDGESESKVKDVEEEDVGTKKDDEGESELGGKVDVDDKSDNVIEEEGVELTDKGDVIVNSSPVESVHVDVAKPGVVVVGDAEGSEELKINADAETLEVANKFDQIGDDDSGEFEPVSDKAIEEVEEKFTSESDSIADSSKLESVDTSAVEPEVVAAESGSEPKDVEKANGLEKGMTYAEVIKAASAVADNGTKEEESVLGGIVDDAEEGVKLNNKGDFVVDSSAIEAVNVDVAKPGVVVVGDVEVSEVLETDGNIPDVHNKFDPIGQGEGGEVELESDKATEEGGGKLVSEGDSMVDSSVVDSVDADINVAEPGVVVVGAAKEAVIKEDDKDDEVDKTISNIEEPDDLTAAYDGNFELAVKEISEAAKVEPDEPKVGVEVEELPVSESLKVGSVDAEEDSIPAAESQFEVRKVVEGDSAEEDENKLPVEDIVSSREFSFGGKEVDQEPSGEGVTRVDGSESEEETEEMIFGSSEAAKQFLAELEKASSGIEAHSDEANISNNMSDRIDGQIVTDSDEDVDTEDEGEEKMFDTAALAALLKAATGGGSSEGGNFTITSQDGTKLFSMDRPAGLSSSLRPLKPAAAPRANRSNIFSNSNVTMADETEINLSEEEKQKLEKLQSLRVKFLRLLQRLGHSAEDSIAAQVLYRLALLAGRQAGQLFSLDAAKKKAVESEAEGNEELIFSLNILVLGKAGVGKSATINSILGNQIASIDAFGLSTTSVREISGTVNGVKITFIDTPGLKSAAMDQSTNAKMLSSVKKVMKKCPPDIVLYVDRLDTQTRDLNNLPLLRTITASLGTSIWKNAIVTLTHAASAPPDGPSGTPLSYDVFVAQCSHIVQQSIGQAVGDLRLMNPSLMNPVSLVENHPLCRKNREGVKVLPNGQTWRSQLLLLCYSLKVLSETNSLLRPQEPLDHRKVFGFRVRSPPLPYLLSWLLQSRAHPKLPGDQGGDSVDSDIEIDDVSDSEQEDGEDDEYDQLPPFKPLRKTQLAKLSNEQRKAYFEEYDYRVKLLQKKQWREELKRMKEMKKNGKKLGESEFGYPGEEDDPENGAPAAVPVPLPDMVLPPSFDSDNSAYRYRYLEPTSQLLTRPVLDTHGWDHDCGYDGVNAEHSLALASRFPATATVQVTKDKKEFNIHLDSSVSAKHGENGSTMAGFDIQNVGKQLAYVVRGETKFKNLRKNKTTVGGSVTFLGENIATGVKLEDQIALGKRLVLVGSTGTMRSQGDSAYGANLEVRLREADFPIGQDQSSFGLSLVKWRGDLALGANLQSQVSVGRNSKIALRAGLNNKMSGQITVRTSSSDQLQIALTAILPIAMSIYKSIRPEATNDKYSMY toc75_pea Protein TOC75, chloroplastic 75 kDa translocon at the outer-envelope membrane of chloroplasts Import intermediate-associated protein of 75 kDa 75 kDa chloroplast outer envelope protein TOC75 IAP75 OEP75 protein protein pea Pisum sativum (Garden pea) Garden pea MRTSVIPNRLTPTLTTHPSRRRNDHITTRTSSLKCHLSPSSGDNNDSFNSSLLKTISTTVAVSSAAASAFFLTGSLHSPFPNFSGLNAAAGGGAGGGGGGSSSSGGGGGGWFNGDEGSFWSRILSPARAIADEPKSEDWDSHELPADITVLLGRLSGFKKYKISDILFFDRNKKSKVETQDSFLDMVSLKPGGVYTKAQLQKELESLATCGMFEKVDMEGKTNADGSLGLTISFAESMWERADRFRCINVGLMGQSKPVEMDPDMSEKEKIEFFRRQEREYKRRISSARPCLLPTSVHEEIKDMLAEQGRVSARLLQKIRDRVQSWYHEEGYACAQVVNFGNLNTREVVCEVVEGDITKLSIQYLDKLGNVVEGNTEGPVVQRELPKQLLPGHTFNIEAGKQALRNINSLALFSNIEVNPRPDEMNEGSIIVEIKLKELEQKSAEVSTEWSIVPGRGGRPTLASLQPGGTITFEHRNLQGLNRSLTGSVTTSNFLNPQDDLAFKMEYAHPYLDGVDNPRNRTLRVSCFNSRKLSPVFTGGPGVDEVPSIWVDRAGVKANITENFSRQSKFTYGLVMEEIITRDESNHICSNGQRVLPNGAISADGPPTTLSGTGIDRMAFLQANITRDNTRFVNGTIVGSRNMFQVDQGLGVGSNFPFFNRHQLTVTKFLQLMSVEEGAGKSPPPVLVLHGHYGGCVGDLPSYDAFTLGGPYSVRGYNMGEIGAARNILELAAEIRIPIKGTHVYAFAEHGTDLGSSKDVKGNPTVVYRRMGQGSSYGAGMKLGLVRAEYAVDHNSGTGAVFFRFGERF toc34_pea Translocase of chloroplast 34 34 kDa chloroplast outer envelope protein GTP-binding protein OEP34 GTP-binding protein IAP34 TOC34 IAP34 OEP34 protein protein pea Pisum sativum (Garden pea) Garden pea MASQQQTVREWSGINTFAPATQTKLLELLGNLKQEDVNSLTILVMGKGGVGKSSTVNSIIGERVVSISPFQSEGPRPVMVSRSRAGFTLNIIDTPGLIEGGYINDMALNIIKSFLLDKTIDVLLYVDRLDAYRVDNLDKLVAKAITDSFGKGIWNKAIVALTHAQFSPPDGLPYDEFFSKRSEALLQVVRSGASLKKDAQASDIPVVLIENSGRCNKNDSDEKVLPNGIAWIPHLVQTITEVALNKSESIFVDKNLIDGPNPNQRGKLWIPLIFALQYLFLAKPIEALIRRDIATETKPAWETRDVGDRK tic55-tic110 BN PAGE and Tricine SDS-PAGE identify components of the Tic translcon. 209738 N/A 209740 unspecified role unspecified role neutral component neutral component pea Pisum sativum (Garden pea) Garden pea N/A 209741 unspecified role unspecified role neutral component neutral component pea Pisum sativum (Garden pea) Garden pea N/A 209742 unspecified role unspecified role neutral component neutral component pea Pisum sativum (Garden pea) Garden pea physical association physical association false false false tic55-tic110-1 Identification of components of the chloroplast import complex 209739 N/A 209743 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea N/A 209744 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea N/A 209745 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea N/A 209741 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea N/A 209742 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea association association false false false This complex was isolated by incubation S35 labelled Rubisco small subunit protein with pea chloroplasts. The identity (e.g. accession numner)of the protein is not specified in the text. Therefore no pSSU protein is entered into this interaction.