IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ baldwin-1994-1 Antiproliferative gastrin/cholecystokinin receptor antagonists target the 78-kDa gastrin-binding protein. in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Cancer - Interactions investigated in the context of cancer Disuccinimidyl-suberate was used as a bifunctional crosslinker. Interaction was performed in the presence of protease inhibitors. Interaction was detected by phosphorimager scanning of PAGE gels. Fig 1 and 2 Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1994 p01350-pro_0000010636 Gastrin GAST GAS protein protein human Homo sapiens Human QGPWLEEEEEAYGWMDF 76 92 echa_pig Trifunctional enzyme subunit alpha, mitochondrial TP-alpha 78 kDa gastrin-binding protein HADHA LCHYD-HAD Monolysocardiolipin acyltransferase protein protein pig Sus scrofa (Pig) Pig MVASRAIGSLRRFTASQTLRCPGYICRNFTRSSALLTRTHINYGVKGDVAVIRINSPNSKVNTLGQELHSEFIEVMNEVWSSSQIRSAVLISSKPGCFIAGADINMLSACTTSQEVTQISQEAQRTFEKLEKSTKPIVAAINGTCLGGGLELAISCQYRIATKDKKTVLGAPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRGIRADKAKKMGLVDQLVEPLGPGLKPPEERTIEYLEEVAVTFAKGLADKKISPKRDKGLVEKLTSYAMSIPFVRQQIYKKVEEKVRKQTKGLYPAPLKIIDVVKTGIEQGSDAGYLSESQKFGELAMTKESKALMGLYRGQTLCKKNKFGAPQKEVKHLAILGAGLMGAGIAQVSVDKHLKTILKDASLPALGRGQQQVFKGLNDKVRKKALTSFERDSLFSNLTGQLDYQGFEKADMVIEAVFEELSLKHKVLKEVEAVIPDHCVFASNTSALPISEIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTEKTSKDSTASAVEVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVLRILQEGVGPKKLDSLTTSFGFPVGAATLMDEVGMDVAKHVAENLGKIFGERFAGGNLDVLKQMISKGFLGRKSGKGFYVYQEGVKNRNVNSDTESILASLKIPSRPDISSDEDIQYRLVTRFVNEAVLCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKVVDRLRKYEAIYGKQFTPCQLLIDHASSPNKKFFQ hadha-gast-1 Interaction between gastrin and GBP studied in the presence of antagonist and analogues 240516 N/A 240517 unspecified role unspecified role neutral component neutral component i125 isotope label isotope label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 240518 unspecified role unspecified role neutral component neutral component pig-gastric mucosa Sus scrofa gastric mucosa gastric_mucosa gastric mucosa direct interaction direct interaction false false false Partially purified HADHA was used. IC50 in the presence of fatty-acid derivatives acetyl-CoA is 0.000045M; and in the presence of crotonyl-CoA is 0.000180M IC50 in the presence of following analogues is 0.0000056 M for CCK8; 0.0000035M for CCK8-Sulphate; 0.0002 M for pentagastrin; 0.000320 M for gastrin4, 0.000250 M for benzotrip.