MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint lee-2005f-1 The FAXWXXT motif in the carboxyl terminus of Vibrio mimicus metalloprotease is involved in binding to collagen. in vitro In vitro experimental interac experimental interaction detection experimental interac predetermined predetermined participant predetermined Lee JH., Ahn SH., Lee EM., Jeong SH., Kim YO., Lee SJ., Kong IS. FEBS Lett. (0014-5793) 2005 2009-01-08 Only protein-protein interactions imex curation lee-2005f-2 The FAXWXXT motif in the carboxyl terminus of Vibrio mimicus metalloprotease is involved in binding to collagen. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom predetermined predetermined participant predetermined Lee JH., Ahn SH., Lee EM., Jeong SH., Kim YO., Lee SJ., Kong IS. FEBS Lett. (0014-5793) 2005 2009-01-08 Only protein-protein interactions imex curation o67990_vibmi Microbial collagenase vmc protein protein vibmi Vibrio mimicus VYSQPLFRRHRIAIACLLAGLSHTTYAQAACDIQDLQQTRDLPAQIAAADESCYSSWFYAPAGTLETVYSEATLSHLQTVLDAEITRYTGEAEQAQRLENYGEFIRAAYYVRYNAESEPYSQALSQRFAQSINRFLLHPHAFDQGREQVAAMKSLSLMVDNIKQLPLTMDAMMSALRLFNQETAKNTQWVDGLNNLFRSMSGHVGNAEFYRYLAANTQHIDTLHQFAIENEWALNTDAAFLVYNALRETGRLLVSPDAVTKQKALRVMEQTLVRYPLGSQHDKIWLAAVDMMRYYAPEALQAQGIDFEVAKQELAARILPNRYECQGPAIIRSQDLSDRQAARACDVLNAKEEDFHQVVNSGYVPVADDNTERVEVAVFANNDSYVNYSAFLFNNTTDNGGQYLEGNPAEANNQARFVAYRYANGDDLSILNLEHEYTHYLDARFNQYGTFSDNLAHGHIVWWLEGFAEYMHYKQGYQAAIELIPQGKMSLSDVMATTYSHDSNRIYRWGYLAVRFMMEKHPQDVESLLVLSRPGQFAQWAQTVKNLGEQYNAEFAVWLDTFKAETPENPDPSEPETKPEEAVTALTANQSVTLSGQAYSEHLFYIDVPEHSREFQVTISGDATPIFT fragmentation type: fragment mint co1a2_bovin Collagen alpha-2(I) chain COL1A2 Alpha-2 type I collagen protein protein bovin Bos taurus (Bovine) Bovine MLSFVDTRTLLLLAVTSCLATCQSLQEATARKGPSGDRGPRGERGPPGPPGRDGDDGIPGPPGPPGPPGPPGLGGNFAAQFDAKGGGPGPMGLMGPRGPPGASGAPGPQGFQGPPGEPGEPGQTGPAGARGPPGPPGKAGEDGHPGKPGRPGERGVVGPQGARGFPGTPGLPGFKGIRGHNGLDGLKGQPGAPGVKGEPGAPGENGTPGQTGARGLPGERGRVGAPGPAGARGSDGSVGPVGPAGPIGSAGPPGFPGAPGPKGELGPVGNPGPAGPAGPRGEVGLPGLSGPVGPPGNPGANGLPGAKGAAGLPGVAGAPGLPGPRGIPGPVGAAGATGARGLVGEPGPAGSKGESGNKGEPGAVGQPGPPGPSGEEGKRGSTGEIGPAGPPGPPGLRGNPGSRGLPGADGRAGVMGPAGSRGATGPAGVRGPNGDSGRPGEPGLMGPRGFPGSPGNIGPAGKEGPVGLPGIDGRPGPIGPAGARGEPGNIGFPGPKGPSGDPGKAGEKGHAGLAGARGAPGPDGNNGAQGPPGLQGVQGGKGEQGPAGPPGFQGLPGPAGTAGEAGKPGERGIPGEFGLPGPAGARGERGPPGESGAAGPTGPIGSRGPSGPPGPDGNKGEPGVVGAPGTAGPSGPSGLPGERGAAGIPGGKGEKGETGLRGDIGSPGRDGARGAPGAIGAPGPAGANGDRGEAGPAGPAGPAGPRGSPGERGEVGPAGPNGFAGPAGAAGQPGAKGERGTKGPKGENGPVGPTGPVGAAGPSGPNGPPGPAGSRGDGGPPGATGFPGAAGRTGPPGPSGISGPPGPPGPAGKEGLRGPRGDQGPVGRSGETGASGPPGFVGEKGPSGEPGTAGPPGTPGPQGLLGAPGFLGLPGSRGERGLPGVAGSVGEPGPLGIAGPPGARGPPGNVGNPGVNGAPGEAGRDGNPGNDGPPGRDGQPGHKGERGYPGNAGPVGAAGAPGPQGPVGPVGKHGNRGEPGPAGAVGPAGAVGPRGPSGPQGIRGDKGEPGDKGPRGLPGLKGHNGLQGLPGLAGHHGDQGAPGAVGPAGPRGPAGPSGPAGKDGRIGQPGAVGPAGIRGSQGSQGPAGPPGPPGPPGPPGPSGGGYEFGFDGDFYRADQPRSPTSLRPKDYEVDATLKSLNNQIETLLTPEGSRKNPARTCRDLRLSHPEWSSGYYWIDPNQGCTMDAIKVYCDFSTGETCIRAQPEDIPVKNWYRNSKAKKHVWVGETINGGTQFEYNVEGVTTKEMATQLAFMRLLANHASQNITYHCKNSIAYMDEETGNLKKAVILQGSNDVELVAEGNSRFTYTVLVDGCSKKTNEWQKTIIEYKTNKPSRLPILDIAPLDIGGADQEIRLNIGPVCFK vmc-col1a2 185142 o67990_vibmi 185144 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 co1a2_bovin 185145 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey purified purified Stoichiometry: 1.0 association association false false false F4B mint col1a2-vmc 185142 o67990_vibmi 185144 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified binding site binding region binding-associated region binding region exp feature detect experimental feature detection exp feature detect certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 co1a2_bovin 185145 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified Stoichiometry: 1.0 association association false false false F6 1) experimental= Scatchard analysis 2.1 picomolar mint col1a2-vmc-1 185143 o67990_vibmi 185144 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey purified purified binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 co1a2_bovin 185145 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified Stoichiometry: 1.0 direct interaction direct interaction false false false F2 mint